BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0312.p (482 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311604|ref|YP_003775696.1| formiminoglutamate deiminase [H... 965 0.0 gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylam... 67 5e-12 gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hy... 66 8e-12 >gi|300311604|ref|YP_003775696.1| formiminoglutamate deiminase [Herbaspirillum seropedicae SmR1] Length = 482 Score = 965 bits (2494), Expect = 0.0, Method: Compositional matrix adjust. Identities = 478/482 (99%), Positives = 481/482 (99%) Query: 1 MASHGDQDLHHLDQAPDNACANGALFCPLALLPQGWRSDVLLRWDGTGRLTHVTPDSARG 60 MASHGDQDLHHLDQAP+NACANGALFCPLALLPQGWRSDVLLRWDG GRLTHVTPDSARG Sbjct: 1 MASHGDQDLHHLDQAPENACANGALFCPLALLPQGWRSDVLLRWDGAGRLTHVTPDSARG 60 Query: 61 SAALASGPVLPGMPNLHSHAFQRAMAGLAETMGSPADSFWSWRELMYRFAQRLQPRQLEA 120 SAALASGPVLPGMPNLHSHAFQRAMAGLAETMGSPADSFWSWRELMYRFAQRLQPRQLEA Sbjct: 61 SAALASGPVLPGMPNLHSHAFQRAMAGLAETMGSPADSFWSWRELMYRFAQRLQPRQLEA 120 Query: 121 IARQTYIEMLKAGYTSVCEFHYLHHAPDGAPYADPAEMAWPLLQAAGQSGIGMTLLPVLY 180 IARQTYIEMLKAGYTSVCEFHYLHHAPDGAPYADPAEMAWPLLQAAGQSGIGMTLLPVLY Sbjct: 121 IARQTYIEMLKAGYTSVCEFHYLHHAPDGAPYADPAEMAWPLLQAAGQSGIGMTLLPVLY 180 Query: 181 QQGGLGGRPVSAEQRRFVAAPSWLLGLRERLLQHMPENDMRRYGVAPHSLRAVAPTGLEE 240 QQGGLGGRPVSAEQRRFVAAPSWLLGLRERLLQHMPENDMRRYGVAPHSLRAVAPTGLEE Sbjct: 181 QQGGLGGRPVSAEQRRFVAAPSWLLGLRERLLQHMPENDMRRYGVAPHSLRAVAPTGLEE 240 Query: 241 LLSGLAGQPGGSDAPIHIHVAEQVREVEDCLAWSGQRPVQWLLSSQPVDARWCLVHATHM 300 LLSGLAGQPGGSDAPIHIHVAEQ+REVEDCLAWSGQRPVQWLLSSQPVDARWCLVHATHM Sbjct: 241 LLSGLAGQPGGSDAPIHIHVAEQLREVEDCLAWSGQRPVQWLLSSQPVDARWCLVHATHM 300 Query: 301 DDEEYRAVAGSGAVVGLCPTTEANLGDGVIDAPRLIDSAARWGIGSDSNIAISLRAELRL 360 DDEEYRAVAGSGAVVGLCPTTEANLGDGVIDAPRLIDSAARWGIGSDSNIAISLRAELRL Sbjct: 301 DDEEYRAVAGSGAVVGLCPTTEANLGDGVIDAPRLIDSAARWGIGSDSNIAISLRAELRL 360 Query: 361 LEYAQRLHHRRRNVLAVVDAPAVADRLFCQALAGGAQAAGRAVAGLMPGQRADLVVLDPD 420 LEYAQRLHHRRRNVLAV+DAPAVADRLFCQALAGGAQAAGRAVAGLMPGQRADLVVLDPD Sbjct: 361 LEYAQRLHHRRRNVLAVIDAPAVADRLFCQALAGGAQAAGRAVAGLMPGQRADLVVLDPD 420 Query: 421 HVNLAQRSSTQLLSALVFCEHDGPMIGDVYVGGRQVVKEGRHALDDRAREDYRHALAALL 480 HVNLAQRSSTQLLSALVFCEHDGPMIGDVYVGGRQVVKEGRHALDDRAREDYRHALAALL Sbjct: 421 HVNLAQRSSTQLLSALVFCEHDGPMIGDVYVGGRQVVKEGRHALDDRAREDYRHALAALL 480 Query: 481 KD 482 KD Sbjct: 481 KD 482 >gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylaminohydrolase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 66.6 bits (161), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 108/432 (25%), Positives = 165/432 (38%), Gaps = 88/432 (20%) Query: 71 PGMPNLHSHAFQRAMAGLAETMGSPADSFWSWRELM-YRFAQRLQPRQLEAIARQTYIEM 129 P N H H FQ + G + +G A + W YR+ AR +E+ Sbjct: 59 PAWVNTHHHLFQSLLKG--DPLGINA-TLTPWLAATPYRYRASFDADLFRLAARIGMVEL 115 Query: 130 LKAGYTSVCEFHYLHHAPDGAPYADPAEMAWPLLQAAGQSGIGM---------------- 173 +++G ++ + +YL++ G P+ D +E+ L + A Q G+ Sbjct: 116 MRSGCGTIADHNYLYYP--GMPF-DTSEI---LFEEAQQLGLRFVLCRGTATRTRQLEAE 169 Query: 174 --------TLLPVLYQQGGLGGR---PVSAEQRRFVAAPSWLLGLRERLLQHMPENDMRR 222 TL L GL GR P A RR V AP+ L Sbjct: 170 LSSALRPETLDGFLADMQGLAGRFHDPAPASMRRVVMAPTTPL----------------- 212 Query: 223 YGVAPHSLRAVAPTGLEELLSGLAGQPGGSDAPIHIHVAEQVREVEDCLAWSGQRPVQWL 282 Y AP LR +A E L +H H++E V + A G RPV++ Sbjct: 213 YSAAPDELREMAVAAREMGLR------------LHSHLSETVGYQDSAHAMHGCRPVEFC 260 Query: 283 LSSQPVDARWCLVHATHMDDEEYRAVAGSGAVVGLCPTTEANLGDGVIDAPRLIDSAARW 342 + H +D EE + + G+G + CP + LG G+ L + Sbjct: 261 ERVGWLGRDVWFAHLVKLDAEEIQLLGGTGTGIAHCPQSNGRLGSGIAPIRALEAAGVPV 320 Query: 343 GIGSD---SNIAISLRAELR---LLEYAQRLHH-----RRRNVLAVVDAPAVADRLFCQA 391 IG D SN A + +E L++ A+R R + DA V D + Sbjct: 321 SIGVDGAASNEAADMISETHAAWLMQRARRGQEATPSFRGGHFEGGADAATVED-VVRWG 379 Query: 392 LAGGAQAAG-RAVAGLMPGQRADLVVL---DPDHVNLAQRSSTQLLSALVFCEHDGPMIG 447 GGA+ G A+ GL GQ+AD+ + DP + L + + S P + Sbjct: 380 TTGGARVLGLDALDGLQVGQQADIALYRLDDPRYFGLHDPAIGPVASG------GRPFLK 433 Query: 448 DVYVGGRQVVKE 459 ++VGGR VV+E Sbjct: 434 AMWVGGRAVVRE 445 >gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hydrolase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 65.9 bits (159), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 95/402 (23%), Positives = 160/402 (39%), Gaps = 41/402 (10%) Query: 69 VLPGMPNLHSHAFQRAMAGLAETMGSPADSFWSWRELMYRFAQRLQPRQLEAIARQTYIE 128 VLPG+ N H H +Q + ++W +Y L P ++ E Sbjct: 64 VLPGLINTHHHMYQSLTRAIPSAQNG---ELFNWLTKLYPIWAGLTPEMIQVSTLTAMAE 120 Query: 129 MLKAGYTSVCEFHYLHHAPDGAPYADPAEMAWPL-LQAAGQSGIGMTLLPVLYQQGGLGG 187 ++ +G T+ + Y++ P+ D E A + ++ G G + V +GGL Sbjct: 121 LILSGCTTSSDHLYIY--PNQTRLDDSLEAAARIGMRFHGARGA----MSVGQSKGGL-- 172 Query: 188 RPVSAEQRRFVAAPSWLLGLRERLLQHMPEND---MRRYGVAPHSLRAVAPTGLEELLSG 244 R V + +L +RL++ ++ M+R VAP S +V+ + E + Sbjct: 173 -----PPDRVVQDEAAILKDTQRLIETYHDHQRHAMQRIVVAPCSPFSVSRELMREA-AD 226 Query: 245 LAGQPGGSDAPIHIHVAEQVREVEDCLAWSGQRPVQWLLSSQPVDARWCLVHATHMDDEE 304 LA G S +H H+AE V ++ P ++ V H +D Sbjct: 227 LARHYGVS---LHTHLAENVNDIAYSREKFNMSPAEYAEDCGWVGHDVWHAHCVQLDAHG 283 Query: 305 YRAVAGSGAVVGLCPTTEANLGDGVIDAPRLIDSAARWGIGSD---SNIAISLRAELRLL 361 A +G V CP + L G+ +++D+ G+G D SN + + E+R Sbjct: 284 IDLFARTGTGVAHCPCSNMRLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHMLGEVRQA 343 Query: 362 EYAQRLHHRRRNVLAVVDAPAVADRLFCQALAGGAQAAGR-AVAGLMPGQRADLVVLDPD 420 QR + A + A GGA+ R + L PG AD+V+ D Sbjct: 344 MLLQR--------VGFGPDAMTARQALEVATLGGAKVLNRDDIGALKPGMSADVVMFDTR 395 Query: 421 HVNLAQRSSTQLLSALVFCEHDGPMIGDV-YVGGRQVVKEGR 461 + A + ++ALVFC P D + GR +V+EG+ Sbjct: 396 QIAFAG-ALHDPVAALVFCT---PANVDCSIINGRVIVREGQ 433 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,709,178 Number of extensions: 81216 Number of successful extensions: 230 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 228 Number of HSP's successfully gapped: 3 Length of query: 482 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 393 Effective length of database: 1,198,455 Effective search space: 470992815 Effective search space used: 470992815 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)