BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0274.p (1286 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310093|ref|YP_003774185.1| transmembrane protein [Herbaspi... 2553 0.0 gi|300313314|ref|YP_003777406.1| transmembrane protein [Herbaspi... 2156 0.0 >gi|300310093|ref|YP_003774185.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1291 Score = 2553 bits (6617), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1275/1291 (98%), Positives = 1277/1291 (98%), Gaps = 5/1291 (0%) Query: 1 MQRLWHFLSDTRTLVIIGFAALAAFLFIGASTLELALTWAAVILAIALLCWLGYWLYRRW 60 MQRLWHFLSDTRTLVIIGFAALAAFLFIGASTLELALTWAAVILAIALLCWLGYWLYRRW Sbjct: 1 MQRLWHFLSDTRTLVIIGFAALAAFLFIGASTLELALTWAAVILAIALLCWLGYWLYRRW 60 Query: 61 KVRRAAGKLGEVLEQQATQSVAASSDGAQREEVAALRTRMLEAINTIKTSKLGQKSGSAA 120 KVRRAAGKLGEVLEQQATQSVAASSDGAQREEVAALRTRMLEAINTIKTSKLGQKSGSAA Sbjct: 61 KVRRAAGKLGEVLEQQATQSVAASSDGAQREEVAALRTRMLEAINTIKTSKLGQKSGSAA 120 Query: 121 LYELPWYMVIGNPAAGKSTAIANSGLQFPFADKGGKIVHGVGGTRNCDWFFTTDGILLDT 180 LYELPWYMVIGNPAAGKSTAIANSGLQFPFADKGGKIVHGVGGTRNCDWFFTTDGILLDT Sbjct: 121 LYELPWYMVIGNPAAGKSTAIANSGLQFPFADKGGKIVHGVGGTRNCDWFFTTDGILLDT 180 Query: 181 AGRYSVYEEDRNEWFSFLGLLKRYRKQAPINGIIIAVSIAELTGNRPEFAINLAKNLRQR 240 AGRYSVYEEDRNEWFSFLGLLKRYRKQAPINGIIIAVSIAELTGNRPEFAINLAKNLRQR Sbjct: 181 AGRYSVYEEDRNEWFSFLGLLKRYRKQAPINGIIIAVSIAELTGNRPEFAINLAKNLRQR 240 Query: 241 VQELTEKLEVFAPVYVVFTKADLITGFNEFFLDTERSERDRVWGATLAYDRKRSGQEVVS 300 VQELTEKLEVFAPVYVVFTKADLITGFNEFFLDTERSERDRVWGATLAYDRKRSGQEVVS Sbjct: 241 VQELTEKLEVFAPVYVVFTKADLITGFNEFFLDTERSERDRVWGATLAYDRKRSGQEVVS 300 Query: 301 FFDERFDELYAGLKEMSLASMSVKRGENMAPGVLTFPLEFSSIKGVLRSFVATLFEENPF 360 FFDERFDELYAGLKEMSLASMSVKRGENMAPGVLTFPLEFSSIKGVLRSFVATLFEENPF Sbjct: 301 FFDERFDELYAGLKEMSLASMSVKRGENMAPGVLTFPLEFSSIKGVLRSFVATLFEENPF 360 Query: 361 QFKPVFRGFYFTSAVQEGATVSASSQRIADRFGLGLEHKPQREIFSQHGFFLHNLFKQVI 420 QFKPVFRGFYFTSAVQEGATVSASSQRIADRFGL LEHKPQREIFSQHGFFLHNLFKQVI Sbjct: 361 QFKPVFRGFYFTSAVQEGATVSASSQRIADRFGLALEHKPQREIFSQHGFFLHNLFKQVI 420 Query: 421 FADKQLVAQYTSRTKIRMRYATFFAAIAILGVLLGGWSWSYMGNRQLLANVQADMDKAVK 480 FADKQLVAQYTSRTKIRMRYATFFAAIAILGVLLGGWSWSYMGNRQLLANVQADMDKAVK Sbjct: 421 FADKQLVAQYTSRTKIRMRYATFFAAIAILGVLLGGWSWSYMGNRQLLANVQADMDKAVK 480 Query: 481 LQEKRLDLQSRFEALEILQDRIEQLDRYRASRPLSVGMGLYQGDLLERKLREEYFSGIKE 540 LQEKRLDLQSRFEALEILQDRIEQLDRYRASRP+SVGMGLYQGDLLERKLREEYFSGIKE Sbjct: 481 LQEKRLDLQSRFEALEILQDRIEQLDRYRASRPISVGMGLYQGDLLERKLREEYFSGIKE 540 Query: 541 IMLKPVASNIESYLMEVNANAARLEPMSRPPQSGGITPVADNGAQAASALRTYKDSSATS 600 IMLKPVASNIESYLMEVNANAARLEPMSRPPQSGGITPVADNGAQAASALRTYKDSSATS Sbjct: 541 IMLKPVASNIESYLMEVNANAARLEPMSRPPQSGGITPVADNGAQAASALRTYKDSSATS 600 Query: 601 VEDAYNALKTYLMLADKSRAESSHLNDQITRFWRGWLETNRGTMPREQMIRSAERLISFS 660 VEDAYNALKTYLMLADKSRAESSHLNDQITRFWRGWLETNRGTMPREQMIRSAERLISFS Sbjct: 601 VEDAYNALKTYLMLADKSRAESSHLNDQITRFWRGWLETNRGTMPREQMIRSAERLISFS 660 Query: 661 LEQINDPSWPTIDNKLTLVDQARENLRRVVRGMPARERVYADVKARAATRFASMTVARIV 720 LEQINDPSWPTIDNKLTLVDQARENLRRVVRGMPARERVYADVKARAATRFASMTVARIV Sbjct: 661 LEQINDPSWPTIDNKLTLVDQARENLRRVVRGMPARERVYADVKARAATRFASMTVARIV 720 Query: 721 GDKDRELVMGSYAIPGTFTRDAWEKFVQEAFKEAANKELQSADWVLKTSAKDDLTLEGSP 780 GDKDRELVMGSYAIPGTFTRDAWEKFVQEAFKEAANKELQSADWVLKTSAKDDLTLEGSP Sbjct: 721 GDKDRELVMGSYAIPGTFTRDAWEKFVQEAFKEAANKELQSADWVLKTSAKDDLTLEGSP 780 Query: 781 EQIQKALVTQYKTEYAREWQKFLQGVSIVELRNLDDATNAMNRLGDPITSPMKKVINTVY 840 EQIQKALVTQYKTEYAREWQKFLQGVSIVELRNLDDATNAMNRLGDPITSPMKKVINTVY Sbjct: 781 EQIQKALVTQYKTEYAREWQKFLQGVSIVELRNLDDATNAMNRLGDPITSPMKKVINTVY 840 Query: 841 DETSWDNPSLVDAGLANAKSGFMGWFKETILRRSPAPAPTQFNADGTTAIPMGPVGREFS 900 DETSWDNPSLVDAGLANAKSGFMGWFKETILRRSPAPAPTQFNADGTTAIPMGPVGREFS Sbjct: 841 DETSWDNPSLVDAGLANAKSGFMGWFKETILRRSPAPAPTQFNADGTTAIPMGPVGREFS 900 Query: 901 GVARLVVSKDKNGSLLQTYLDNLSKLRTRLNTIKNQGDTGPGAKQLMQQTLEGNGSELSD 960 GVARLVVSKDKNGSLLQTYLDNLSKLRTRLNTIKNQGDTGPGAKQLMQQTLEGNGSELSD Sbjct: 901 GVARLVVSKDKNGSLLQTYLDNLSKLRTRLNTIKNQGDTGPGAKQLMQQTLEGNGSELSD 960 Query: 961 SLKFVDEEMLPGLNDQQKTVLRPLLVRPLVQGFNALVRPTEGEVNKIWRAQVYEPFQSNL 1020 SLKFVDEEMLPGLNDQQKTVLRPLLVRPLVQGFNALVRPTEGEVNKIWRAQVYEPFQSNL Sbjct: 961 SLKFVDEEMLPGLNDQQKTVLRPLLVRPLVQGFNALVRPTEGEVNKIWRAQVYEPFQSNL 1020 Query: 1021 AAKYPFSPNSKIEANSGEIGTVFGENGAISKFVTTAMGPLVVRRGDTLSPRKWADMGINL 1080 AAKYPFSPNSKIEANSGEIGTVFGENGAISKFVTTAMGPLVVRRGDTLSPRKWADMGINL Sbjct: 1021 AAKYPFSPNSKIEANSGEIGTVFGENGAISKFVTTAMGPLVVRRGDTLSPRKWADMGINL 1080 Query: 1081 SPAITSNFASWISAP-GGGEANGGANSAQTVFQIQPQPAPGALEYTIEIDGQQLRYRNTQ 1139 SPAITSNFASWISAP GGG+ANGGANSAQTVFQIQPQPAPGALEYTIEIDGQQLRYRNTQ Sbjct: 1081 SPAITSNFASWISAPGGGGDANGGANSAQTVFQIQPQPAPGALEYTIEIDGQQLRYRNTQ 1140 Query: 1140 AQWTNFIWPNPQGTPGARVTAQTYDGRTIEVASQPGRFGLERLINSAQRKRKDNGVFELS 1199 AQWTNFIWPNPQGTPGARVTAQTYDGRTIEVASQPGRFGLERLINSAQRKRKDNGVFELS Sbjct: 1141 AQWTNFIWPNPQGTPGARVTAQTYDGRTIEVASQPGRFGLERLINSAQRKRKDNGVFELS 1200 Query: 1200 WTNEGVTVVVNLKIISSAEVSGTANGPARGTASLRGLKLPETIAG----GAPAPTPAGAA 1255 WTNEGVTVVVNLKIISSAEVSGTANGPARGTASLRGLKLPETIAG AP PAGAA Sbjct: 1201 WTNEGVTVVVNLKIISSAEVSGTANGPARGTASLRGLKLPETIAGGAPAPVLAPAPAGAA 1260 Query: 1256 PMQPMQPAQPMATDAAAPQSNNQPTITGVKQ 1286 PMQP QPMATDAAAPQSNNQPTITGVKQ Sbjct: 1261 GTAPMQPGQPMATDAAAPQSNNQPTITGVKQ 1291 >gi|300313314|ref|YP_003777406.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1266 Score = 2156 bits (5587), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1065/1250 (85%), Positives = 1145/1250 (91%), Gaps = 2/1250 (0%) Query: 1 MQRLWHFLSDTRTLVIIGFAALAAFLFIGASTLELALTWAAVILAIALLCWLGYWLYRRW 60 MQRLW LSDTR L ++GF ALAA LFIGA LELA W AV+ LL WL Y+RW Sbjct: 1 MQRLWKILSDTRVLTLLGFMALAAVLFIGAEQLELAPIWPAVLFCALLLAWLALRRYQRW 60 Query: 61 KVRRAAGKLGEVLEQQATQSVAASSDGAQREEVAALRTRMLEAINTIKTSKLGQKSGSAA 120 K +RAA KL VLEQQA Q + S+ A+ ++VAALR RMLEAINTIKTSKLGQKSG+AA Sbjct: 61 KAQRAATKLENVLEQQAAQPLPVDSEAARHDDVAALRQRMLEAINTIKTSKLGQKSGAAA 120 Query: 121 LYELPWYMVIGNPAAGKSTAIANSGLQFPFADKGGKIVHGVGGTRNCDWFFTTDGILLDT 180 LYELPWYMVIGNPAAGKSTAIANSGLQFPFADKGGKIVHGVGGTRNCDWFFTTDGILLDT Sbjct: 121 LYELPWYMVIGNPAAGKSTAIANSGLQFPFADKGGKIVHGVGGTRNCDWFFTTDGILLDT 180 Query: 181 AGRYSVYEEDRNEWFSFLGLLKRYRKQAPINGIIIAVSIAELTGNRPEFAINLAKNLRQR 240 AGRYSVYEEDR EWFSFLGLLKRYRKQAPINGIIIAVSIAELTGNRPEFAINLAKNLRQR Sbjct: 181 AGRYSVYEEDRREWFSFLGLLKRYRKQAPINGIIIAVSIAELTGNRPEFAINLAKNLRQR 240 Query: 241 VQELTEKLEVFAPVYVVFTKADLITGFNEFFLDTERSERDRVWGATLAYDRKRSGQEVVS 300 VQELTEKLEVFAPVYVVFTK DLITGFNEFFLD+ER ERDRVWGATLAYDRK SGQEV + Sbjct: 241 VQELTEKLEVFAPVYVVFTKTDLITGFNEFFLDSERGERDRVWGATLAYDRKHSGQEVSN 300 Query: 301 FFDERFDELYAGLKEMSLASMSVKRGENMAPGVLTFPLEFSSIKGVLRSFVATLFEENPF 360 FFDERFDELYAGLKEMSLA+M+VKRGEN+APGVLTFPLEFS+ KGVLRSFV TLFEENPF Sbjct: 301 FFDERFDELYAGLKEMSLANMAVKRGENLAPGVLTFPLEFSAFKGVLRSFVTTLFEENPF 360 Query: 361 QFKPVFRGFYFTSAVQEGATVSASSQRIADRFGLGLEHKPQREIFSQHGFFLHNLFKQVI 420 QFKPVFRGFYFTSA+QEGAT+ AS+QRIADRFGL LE +PQRE+ S+ GFFLHNLFKQVI Sbjct: 361 QFKPVFRGFYFTSALQEGATLGASAQRIADRFGLTLEDQPQREVLSKQGFFLHNLFKQVI 420 Query: 421 FADKQLVAQYTSRTKIRMRYATFFAAIAILGVLLGGWSWSYMGNRQLLANVQADMDKAVK 480 FADKQLVAQYTSR KIRMRY TFFAA+A+LG LLGGWSWSYMGNRQL+ANVQADMDKA+K Sbjct: 421 FADKQLVAQYTSRNKIRMRYLTFFAALAMLGALLGGWSWSYMGNRQLVANVQADMDKAIK 480 Query: 481 LQEKRLDLQSRFEALEILQDRIEQLDRYRASRPLSVGMGLYQGDLLERKLREEYFSGIKE 540 LQEKRLDLQSRFEALEILQDRIEQLDRYR+ RP+S+ +GLYQGDLLERKLREEYF+GIKE Sbjct: 481 LQEKRLDLQSRFEALEILQDRIEQLDRYRSHRPVSIDLGLYQGDLLERKLREEYFAGIKE 540 Query: 541 IMLKPVASNIESYLMEVNANAARLEPMSRPPQSGGITPVADNGAQAASALRTYKDSSATS 600 IMLKPVAS+IE+YL+EVNAN+++LEPMSRPPQ+ + DN A +ALRTYKDSSAT+ Sbjct: 541 IMLKPVASSIENYLIEVNANSSKLEPMSRPPQADSLATTGDNNIHAGNALRTYKDSSATN 600 Query: 601 VEDAYNALKTYLMLADKSRAESSHLNDQITRFWRGWLETNRGTMPREQMIRSAERLISFS 660 VEDAYNALKTYLML+DKSRAE+SHLNDQITRFWRGWLETNRGTMPREQMIRSAERLISFS Sbjct: 601 VEDAYNALKTYLMLSDKSRAEASHLNDQITRFWRGWLETNRGTMPREQMIRSAERLISFS 660 Query: 661 LEQINDPSWPTIDNKLTLVDQARENLRRVVRGMPARERVYADVKARAATRFASMTVARIV 720 LEQI+DPSWPT +NKLTLVDQ RENLR VVRGMPARERVYADVKARAATRFASMTVARIV Sbjct: 661 LEQIDDPSWPTTENKLTLVDQTRENLRHVVRGMPARERVYADVKARAATRFASMTVARIV 720 Query: 721 GDKDRELVMGSYAIPGTFTRDAWEKFVQEAFKEAANKELQSADWVLKTSAKDDLTLEGSP 780 GDKDRELVMGSYAIPGTFTR+AWEKFV++AFKEAANKELQSADWVLKTS+K+DLTLEGSP Sbjct: 721 GDKDRELVMGSYAIPGTFTRNAWEKFVEDAFKEAANKELQSADWVLKTSSKEDLTLEGSP 780 Query: 781 EQIQKALVTQYKTEYAREWQKFLQGVSIVELRNLDDATNAMNRLGDPITSPMKKVINTVY 840 EQIQKALV+QYK+EYAREWQKFLQGVSIVEL NLDDATNAMNRLGDP+TSP+ KVI+TVY Sbjct: 781 EQIQKALVSQYKSEYAREWQKFLQGVSIVELHNLDDATNAMNRLGDPLTSPINKVISTVY 840 Query: 841 DETSWDNPSLVDAGLANAKSGFMGWFKETILRRS--PAPAPTQFNADGTTAIPMGPVGRE 898 DETSWDNPSL+ +GLA A G GWFKET+LRRS P PAP Q N DG AIPMGPV RE Sbjct: 841 DETSWDNPSLIGSGLATASRGLSGWFKETVLRRSPAPPPAPPQLNTDGGAAIPMGPVARE 900 Query: 899 FSGVARLVVSKDKNGSLLQTYLDNLSKLRTRLNTIKNQGDTGPGAKQLMQQTLEGNGSEL 958 F+GVARLVVSKDK SL++ Y++NLSKLRTRLNTIKNQGDTGPGAKQLMQQTL+G+GSEL Sbjct: 901 FAGVARLVVSKDKGNSLMRGYMENLSKLRTRLNTIKNQGDTGPGAKQLMQQTLDGSGSEL 960 Query: 959 SDSLKFVDEEMLPGLNDQQKTVLRPLLVRPLVQGFNALVRPTEGEVNKIWRAQVYEPFQS 1018 SD+LKFVDEEMLPGLNDQQKT LRPLLVRPLVQGFNALV PTEGE+NKIWRAQVYEPFQ+ Sbjct: 961 SDALKFVDEEMLPGLNDQQKTTLRPLLVRPLVQGFNALVHPTEGELNKIWRAQVYEPFQN 1020 Query: 1019 NLAAKYPFSPNSKIEANSGEIGTVFGENGAISKFVTTAMGPLVVRRGDTLSPRKWADMGI 1078 NLA KYPFSP+SKIEANSGEI TVFGE+GAISKFVTTAMGPLVVRRGDTLSPRKWADMGI Sbjct: 1021 NLATKYPFSPSSKIEANSGEINTVFGESGAISKFVTTAMGPLVVRRGDTLSPRKWADMGI 1080 Query: 1079 NLSPAITSNFASWISAPGGGEANGGANSAQTVFQIQPQPAPGALEYTIEIDGQQLRYRNT 1138 LSPAIT++FA WIS G A GGA AQTVFQIQPQPAPGALEYTIEIDGQQLRYRNT Sbjct: 1081 TLSPAITTSFADWISTHGSTGAGGGAGDAQTVFQIQPQPAPGALEYTIEIDGQQLRYRNT 1140 Query: 1139 QAQWTNFIWPNPQGTPGARVTAQTYDGRTIEVASQPGRFGLERLINSAQRKRKDNGVFEL 1198 QAQW NF+WPNPQG+PGARVTA TYDGRT+EVASQPGRFGLERLIN+AQRKRKDNG FEL Sbjct: 1141 QAQWVNFVWPNPQGSPGARVTAVTYDGRTVEVASQPGRFGLERLINTAQRKRKDNGAFEL 1200 Query: 1199 SWTNEGVTVVVNLKIISSAEVSGTANGPARGTASLRGLKLPETIAGGAPA 1248 SWTNEGVTV+V+LKIISSAEVSG +G ARGTA LRGLKLPETIAGG A Sbjct: 1201 SWTNEGVTVMVSLKIISSAEVSGNGSGTARGTAGLRGLKLPETIAGGGSA 1250 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,696,276 Number of extensions: 151231 Number of successful extensions: 518 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 515 Number of HSP's successfully gapped: 2 Length of query: 1286 Length of database: 1,619,870 Length adjustment: 97 Effective length of query: 1189 Effective length of database: 1,160,575 Effective search space: 1379923675 Effective search space used: 1379923675 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 136 (57.0 bits)