BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0270.p (689 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310085|ref|YP_003774177.1| hypothetical protein Hsero_0750... 1311 0.0 gi|300312698|ref|YP_003776790.1| hypothetical protein Hsero_3401... 64 3e-11 gi|300313107|ref|YP_003777199.1| transmembrane protein [Herbaspi... 64 5e-11 gi|300312704|ref|YP_003776796.1| transmembrane protein [Herbaspi... 64 6e-11 >gi|300310085|ref|YP_003774177.1| hypothetical protein Hsero_0750 [Herbaspirillum seropedicae SmR1] Length = 1070 Score = 1311 bits (3394), Expect = 0.0, Method: Compositional matrix adjust. Identities = 628/636 (98%), Positives = 630/636 (99%) Query: 1 MGTEVTQVKSTTGAATMRANANSAQKCALGSNGQCSFCQRTGYPILPLRYAVKPSYVTAI 60 MGTEVTQVKSTTGAATMRANANSAQKCALGSNGQCSFCQRTGYPILPLRYAVKPSYVTAI Sbjct: 1 MGTEVTQVKSTTGAATMRANANSAQKCALGSNGQCSFCQRTGYPILPLRYAVKPSYVTAI 60 Query: 61 GAGPQSLPQMDKFTAQPLKGNRYTLRVLRKGYVHVYMGVKGHWQTYAVTDDGYLRLLKNP 120 GAGPQSLPQMDKF AQPLKGNRYTLRVLRKGYVHVYMGVKGHWQTYAVTDDGYLRLLKNP Sbjct: 61 GAGPQSLPQMDKFAAQPLKGNRYTLRVLRKGYVHVYMGVKGHWQTYAVTDDGYLRLLKNP 120 Query: 121 DDPDAKGNRPLTEACKRDGHNIPASFINIPEKYKKVWIGFSELPWRKEVRSAFEAKPDAR 180 DDPDAKGNRPLTEACKRDGHNIPASFINIPEKYKKVWIGFSELPWRKEVRSAFEAKPDAR Sbjct: 121 DDPDAKGNRPLTEACKRDGHNIPASFINIPEKYKKVWIGFSELPWRKEVRSAFEAKPDAR 180 Query: 181 MQQVDLTQMANAPAQVPNALEITPDGTSLHAYVEEYARDAGDYAKRLQYPAPVVSAEKVS 240 MQQVDLTQMANAPAQVPNALEITPDGTSLHAYVEEYARDAGDYAKRLQYPAPVVSAEKVS Sbjct: 181 MQQVDLTQMANAPAQVPNALEITPDGTSLHAYVEEYARDAGDYAKRLQYPAPVVSAEKVS 240 Query: 241 KGDFQWESLHGNYPRSGQLAAVGEHATAYQKKTKRKMAALVLHDPVGMVQELNGAVSRVV 300 KGDFQWESLHGNYPRSGQLAAVGEHATAYQKKTKRKMAALVLHDPVGMVQELNGAVSRVV Sbjct: 241 KGDFQWESLHGNYPRSGQLAAVGEHATAYQKKTKRKMAALVLHDPVGMVQELNGAVSRVV 300 Query: 301 KARQDFGAAVMRPLIVSQSILGLKKFFEQAASANREAQEKKDKKPDKQYELDTESIPMSG 360 KARQDFGAAVMRPLIVSQSILGLKKFFEQAASANREAQEKKDKKPDKQYELDTESIPMSG Sbjct: 301 KARQDFGAAVMRPLIVSQSILGLKKFFEQAASANREAQEKKDKKPDKQYELDTESIPMSG 360 Query: 361 TAGISVYNMKTTTRAERAQRDADDAWIRIARRYDEGARAAFDQRYTEAMKGFEEGIKNQG 420 TAGISVYNMKTTTRAERAQRDADDAWIRIARRYDEGARAAFDQRYTEAMKGFEEGIKNQG Sbjct: 361 TAGISVYNMKTTTRAERAQRDADDAWIRIARRYDEGARAAFDQRYTEAMKGFEEGIKNQG 420 Query: 421 HDWALWAGHDAWRLHFKDYVTNYGSDYKDFVEMSAVCLSGGTGEDEYSQALWMKWFSAKP 480 HDWALWAGHDAWRLHFKDYVTNYGSDYKDFVEMSAVCLSGGTGEDEYSQALWMKWFSAKP Sbjct: 421 HDWALWAGHDAWRLHFKDYVTNYGSDYKDFVEMSAVCLSGGTGEDEYSQALWMKWFSAKP 480 Query: 481 DDVTNPVFRGLFGGRKELLAFLLPEKPSLWSEDNELNKTDKLYETAKGITASEEVDGPKK 540 DDVTNPVF+GLFGGRKELLAFLLPEKPSLWSEDNELNKTDKLYETAKGITASEEVDGPKK Sbjct: 481 DDVTNPVFQGLFGGRKELLAFLLPEKPSLWSEDNELNKTDKLYETAKGITASEEVDGPKK 540 Query: 541 LEHFIDDRVKTWAASLMTTISAVSTRVDAKLSQVASLAVNRAIQAGQYLYSGISTTFIKV 600 LEHFID RVKTWAASLMTTISAVSTRVDAKLSQVA L VNRAIQAGQYLYSGISTTFIKV Sbjct: 541 LEHFIDGRVKTWAASLMTTISAVSTRVDAKLSQVALLTVNRAIQAGQYLYSGISTTFIKV 600 Query: 601 RMTVGDYLKLLNELGTKVRDGMRKRVNSLLFAGSLT 636 RMTVGDYLKLLNELG KVRDGMRKRVNSLLFAG L+ Sbjct: 601 RMTVGDYLKLLNELGMKVRDGMRKRVNSLLFAGFLS 636 >gi|300312698|ref|YP_003776790.1| hypothetical protein Hsero_3401 [Herbaspirillum seropedicae SmR1] Length = 829 Score = 64.3 bits (155), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 131/574 (22%), Positives = 210/574 (36%), Gaps = 92/574 (16%) Query: 32 NGQCSFCQRTGYPILPLRYAVKPSYVTAIGAGPQSLPQMD---KFTAQPLKGN--RYTLR 86 NG C+FC + G I P+RYAV Y G +P + K P +Y+LR Sbjct: 2 NG-CNFCDKNGLLIYPVRYAVASPY------GATGVPGLSGNFKIEGAPQSAGAAKYSLR 54 Query: 87 VLRKGYVHVYMGVKGHWQTYAVTDDGYL---RLLKNPDDPDAKGNRPLTEACKRDGHNIP 143 LR GY++ Y + + Y V G+L L NP P L AC G + Sbjct: 55 ALRPGYLYTYDEKRRRLKAYVVMPTGHLWHYPLEYNPPHP-----AKLRFACTDPGELVR 109 Query: 144 ASFINIPEKYK----KVWIGFSELPWRKEVRSAFEAKPDA-----RMQQVDLTQMANAPA 194 A ++IP WIG+S + W K + + DA MQ +D+ M A Sbjct: 110 AYCVDIPHTASDPAGNFWIGWSNVQWTKAL---LKKVSDAGWRKKHMQCIDIPAMLAGSA 166 Query: 195 QVPNALEITPDGTSLHAYVEEYARDAGDYAKRLQYP-APVVSAEKVSKGDFQWESLHGNY 253 + D V + D K + P+ S + +++++ Sbjct: 167 VHTGEFNASADK------VAHFVADEAAMKKAYAFSNTPIESETRKLATAVRFKAI---- 216 Query: 254 PRSGQLAAVGEHATAYQKKTKRKMAALVLHDPVGMVQELNGAVSRVVKARQD--FGAAVM 311 +A G H Y +AA L+DPVGM + + ++A D M Sbjct: 217 -----MAEHGPHHKGY-------IAA--LNDPVGMTNDWSELTMPDLQAGFDEKLHQRKM 262 Query: 312 RPLIVSQSILGLKKFFEQAASANREAQEKKDKKPDKQYELDTESI-PMSGTAGISVYNMK 370 +++ + +++ Q + E PD +++ M GI + K Sbjct: 263 IADLLALTEHHVRQEARQEVVFSDAVAEASAHHPDGDIYNGLKTLGAMFKLGGIDRHEKK 322 Query: 371 TTTR--------AERAQRDADDAWIRIAR-----RYDEGARAAFDQRYTEAMKGFEEGIK 417 + A R Q ADDAW + DE A AF Y A+K FE + Sbjct: 323 LQEQRKKYGEDLAGRQQAAADDAWHELTHDNGKPTLDEAAMKAFPAIYDAALKQFEPQLL 382 Query: 418 NQGHDWALWAGHDAWRLHFKDYVTNYGSDYKDFVEMSAVCLSGGTGEDEYSQALWMKWFS 477 L H W + +++ + V A SG + SQ + K Sbjct: 383 Q------LLMAHLGW------LKSEQLANWMEGVHDDADIRSGYAYSESMSQCIG-KAVC 429 Query: 478 AKP--DDVTNPVFRGLFGGRKELL--AFLLPEKPSLWSEDNELNKTDKLYETAKGI--TA 531 +KP D + + + G ++ L A LL + + + + + +D E + A Sbjct: 430 SKPCIDQLASWISSGNLQDKRNLYVRALLLNQADIIAATETQFKGSDFQLENILHVYKGA 489 Query: 532 SEEVDGPKKLEHFIDDRVKTWAASLMTTISAVST 565 +D PK+ E ID T ++ IS S+ Sbjct: 490 VMRLDDPKQAEQLIDRLALTTGNVIVKAISGSSS 523 >gi|300313107|ref|YP_003777199.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 833 Score = 63.5 bits (153), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 53/206 (25%), Positives = 83/206 (40%), Gaps = 25/206 (12%) Query: 35 CSFCQRTGYPILPLRYAVKPSYVTAIGAGPQSLPQMD-----KFTAQPLKGNRYTLRVLR 89 C++C + G I P+RYAV Y G Q++P + + Q + RYTLR LR Sbjct: 4 CNYCDKRGLLIYPVRYAVASPY------GAQAVPALSGNFRIEGAPQQIGEARYTLRALR 57 Query: 90 KGYVHVYMGVKGHWQTYAVTDDGYLRLLKNPDDPDAKGNRPLTEACKRDGHNIPASFINI 149 GY++ Y + + Y V G+L P G P + C G + A ++I Sbjct: 58 PGYLYTYDERRARLKAYVVMPTGHLWNFPLEYTPPHPGRIPFS--CIDPGEIVRAYCVDI 115 Query: 150 ----PEKYKKVWIGFSELPWRKEVRSAFEAKP--DARMQQVDLTQMANAPAQVPNALEIT 203 + + WIG+S W K ++ MQ +D+ M A P+ E Sbjct: 116 AHTPSDPATRFWIGWSNTTWTKALQKKVSETNWRKKHMQCIDVAAMLAGTA--PHTGEFN 173 Query: 204 PDGTSLHAYVEEYARDAGDYAKRLQY 229 +V +A D + AK + Sbjct: 174 AS----KQHVAHFAADVSEMAKAFSF 195 >gi|300312704|ref|YP_003776796.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 837 Score = 63.5 bits (153), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 73/276 (26%), Positives = 109/276 (39%), Gaps = 49/276 (17%) Query: 32 NGQCSFCQRTGYPILPLRYAVKPSYVTAIGAGPQSLPQMDKFTAQPLKGN--RYTLRVLR 89 NG C+FC + G I P+RYAV Y AG L K P +Y+LR LR Sbjct: 2 NG-CNFCDKKGLLIYPVRYAVASPYG---AAGVPGLSGNFKIEGAPQSAGAAKYSLRALR 57 Query: 90 KGYVHVYMGVKGHWQTYAVTDDGYL---RLLKNPDDPDAKGNRPLTEACKRDGHNIPASF 146 GY++ Y +G + Y V G+L L NP P L AC G + A Sbjct: 58 PGYLYTYDEKRGRLKAYVVMPTGHLWHYPLEYNPPHP-----AKLRFACTDPGELVRAYC 112 Query: 147 INIPEKYK----KVWIGFSELPWRKEVRSAFEAKPDA-----RMQQVDLTQMANAPAQVP 197 ++IP WIG+S + W K + + DA MQ +D+ M A Sbjct: 113 VDIPHTASDPAGNFWIGWSNVQWTKAL---LKKVSDAGWRKKHMQCIDIPAMLAGSAVHT 169 Query: 198 NALEITPDGTSLHAYVEEYARDAGDYAKRLQYPAPVVSAEKVSKGDFQWESLHGNYPRSG 257 + D + E + A ++ P+ S + +++++ Sbjct: 170 GEFNASADKVAHFVADEAAMKKAYAFSN-----TPIESETRKLATAVRFKAI-------- 216 Query: 258 QLAAVGEHATAYQKKTKRKMAALVLHDPVGMVQELN 293 +A G H Y +AA L+DPVGM +L+ Sbjct: 217 -MAEHGPHHKGY-------IAA--LNDPVGMTNDLS 242 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,204,106 Number of extensions: 94634 Number of successful extensions: 238 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 235 Number of HSP's successfully gapped: 4 Length of query: 689 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 597 Effective length of database: 1,184,250 Effective search space: 706997250 Effective search space used: 706997250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)