BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0246.p (202 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310060|ref|YP_003774152.1| nicotinamidase-like protein [He... 395 e-111 gi|300310740|ref|YP_003774832.1| isochorismatase family protein ... 60 1e-10 gi|300310658|ref|YP_003774750.1| 2,3-dihydro-2,3-dihydroxybenzoa... 59 3e-10 gi|300310742|ref|YP_003774834.1| cysteine hydrolase falmily prot... 58 5e-10 gi|300313872|ref|YP_003777964.1| 2,3-dihydro-2,3-dihydroxybenzoa... 56 2e-09 >gi|300310060|ref|YP_003774152.1| nicotinamidase-like protein [Herbaspirillum seropedicae SmR1] Length = 202 Score = 395 bits (1015), Expect = e-111, Method: Compositional matrix adjust. Identities = 200/201 (99%), Positives = 200/201 (99%) Query: 1 MSTPQTLIQIAGGSPRAATWQEAALVIIDHQTEYTTGRAPLAGIGAAVAEVALLLEHARA 60 MSTPQTLIQIAGGSPRAATWQEAALVIIDHQTEYTTGRAPLAGIGAAVAEVALLLEHARA Sbjct: 1 MSTPQTLIQIAGGSPRAATWQEAALVIIDHQTEYTTGRAPLAGIGAAVAEVALLLEHARA 60 Query: 61 AAAPVIHVVHHGKPGGAMFDPQGPHVAIIDGVQPQPGEAVITKGLPNSFAGTTLDETLKK 120 AAAPVIHVVHHGKPGGAMFDPQGPHVAIIDGVQPQPGEAVITKGLPNSFAGTTLDETLKK Sbjct: 61 AAAPVIHVVHHGKPGGAMFDPQGPHVAIIDGVQPQPGEAVITKGLPNSFAGTTLDETLKK 120 Query: 121 TGRKSLIIAGFATHMCVSATVRSALDHGYACTVVAAACATRDLPDPLGGTALSAAEIQRV 180 TGRKSLIIAGFATHMCVSATVRSALDHGYACTVVAAACATRDLPDPLGG ALSAAEIQRV Sbjct: 121 TGRKSLIIAGFATHMCVSATVRSALDHGYACTVVAAACATRDLPDPLGGPALSAAEIQRV 180 Query: 181 ALAELADRYATVVAHAGLLPA 201 ALAELADRYATVVAHAGLLPA Sbjct: 181 ALAELADRYATVVAHAGLLPA 201 >gi|300310740|ref|YP_003774832.1| isochorismatase family protein [Herbaspirillum seropedicae SmR1] Length = 227 Score = 60.1 bits (144), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 54/171 (31%), Positives = 72/171 (42%), Gaps = 31/171 (18%) Query: 21 QEAALVIIDHQTEYTT--GRAPLAG-----IGAAVAEVALLLEHARAAAAPVIHVVH--- 70 + ALV+ID Q ++ G G +GA V VA LL AR V+H Sbjct: 16 RSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLALARQTQMLVVHTRESHL 75 Query: 71 ------------HGKPGGAMFD--PQG-------PHVAIIDGVQPQPGEAVITKGLPNSF 109 G P + D P G P I+ + P GE VI K +F Sbjct: 76 PDLSDCPRTKRLRGNPTLGIGDVGPMGRILVRGEPGNQILPQLTPMAGEIVIDKPGKGAF 135 Query: 110 AGTTLDETLKKTGRKSLIIAGFATHMCVSATVRSALDHGYACTVVAAACAT 160 T L L++ G L++AG T +CV ++R A D GY C VV ACA+ Sbjct: 136 YATDLHAQLQERGITHLLVAGVTTEVCVQTSMREANDRGYECLVVEDACAS 186 >gi|300310658|ref|YP_003774750.1| 2,3-dihydro-2,3-dihydroxybenzoate synthetase [Herbaspirillum seropedicae SmR1] Length = 207 Score = 58.9 bits (141), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 53/183 (28%), Positives = 80/183 (43%), Gaps = 26/183 (14%) Query: 25 LVIIDHQTEYTTGRAPLAG-IGAAVAEVALLLEHARAAAAPVIH-------------VVH 70 L+I+D + + G I A+ LL HAR PV H + Sbjct: 25 LLIVDFVNSFADPQQFGGGNIPQAIERTRSLLAHARQQGWPVAHSRIVFADDDADRNIFG 84 Query: 71 HGKPGGAMFDPQGPHVAIIDGVQPQPGEAVITKGLPNSFAGTTLDETLKKTGRKSLIIAG 130 PG P AI+ + P GE V+ K +P++F GT+L L + G ++L++AG Sbjct: 85 MKVPGMVTLKEDLPGSAIVPQLAPANGELVVRKTVPSAFFGTSLAPWLTQHGVQTLLVAG 144 Query: 131 FATHMCVSATVRSALDHGYACTVVAAACATRDLPDPLGGTALSAAEIQRVALAELADRYA 190 T CV A+V A+ G+ VV+ D +G AL E L ++A +YA Sbjct: 145 AVTSGCVRASVVDAMSWGFRPLVVS---------DCVGDRALGPHEAN---LFDMAQKYA 192 Query: 191 TVV 193 V+ Sbjct: 193 AVM 195 >gi|300310742|ref|YP_003774834.1| cysteine hydrolase falmily protein [Herbaspirillum seropedicae SmR1] Length = 231 Score = 58.2 bits (139), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 53/213 (24%), Positives = 81/213 (38%), Gaps = 51/213 (23%) Query: 22 EAALVIIDHQTEYTTGRAPLAGIGAAVAEVALLLEHARAAAAPVIHVVHHGKPGGAMF-- 79 AL++ID QT++ GIG V ++ L RA P+ V+ + GG Sbjct: 23 NTALIVIDMQTDF-------CGIGGYVDKMGYDLSLTRAPIEPIRKVLAAMRAGGYTIIH 75 Query: 80 ---------------------------------DPQG-------PHVAIIDGVQPQPGEA 99 P G P II + P GE Sbjct: 76 TREGHRPDLSDLPANKRWRSRQIGANGVGIGDAGPCGRILVRGEPGWEIIPELAPIAGEI 135 Query: 100 VITKGLPNSFAGTTLDETLKKTGRKSLIIAGFATHMCVSATVRSALDHGYACTVVAAACA 159 +I K SF T L+ L G +++++ G T +CV T+R A D G+ C +++ C Sbjct: 136 IIDKPGKGSFCATDLEMILHTRGIRNIVLTGITTDVCVHTTMREANDRGFECVMLSDCCG 195 Query: 160 TRDLPDPLGGTALSAAEIQRVALAELADRYATV 192 D + L ALS ++Q +AD A + Sbjct: 196 ATDYNNHL--AALSMIKMQGGVFGAVADSAALI 226 >gi|300313872|ref|YP_003777964.1| 2,3-dihydro-2,3-dihydroxybenzoate synthetase [Herbaspirillum seropedicae SmR1] Length = 176 Score = 56.2 bits (134), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 45/137 (32%), Positives = 61/137 (44%), Gaps = 3/137 (2%) Query: 24 ALVIIDHQTEYTTGRAPLAGIGAAVAEVALLLEHARAAAAPVIHVVHHGKPGGAMFDPQG 83 AL+IID Q G + A + V L + ARAA APVI V H + Sbjct: 4 ALLIIDIQHALCHGPDAVENSAAVIDRVNQLADQARAAGAPVIFVQHEDEDA---LQRGS 60 Query: 84 PHVAIIDGVQPQPGEAVITKGLPNSFAGTTLDETLKKTGRKSLIIAGFATHMCVSATVRS 143 ++ G+ QP + + K ++F T L LK +L+I G T CV +TVR Sbjct: 61 EGWQLMPGLHQQPEDLHLPKRGSDAFHHTELQAMLKMRHISALVICGMQTEYCVESTVRR 120 Query: 144 ALDHGYACTVVAAACAT 160 AL GY + A A T Sbjct: 121 ALALGYPVMLAADAHTT 137 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 570,772 Number of extensions: 18018 Number of successful extensions: 87 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 87 Number of HSP's successfully gapped: 5 Length of query: 202 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 121 Effective length of database: 1,236,335 Effective search space: 149596535 Effective search space used: 149596535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)