BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0197.p (665 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspiri... 1333 0.0 gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspiri... 451 e-127 gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspiri... 419 e-118 gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum... 412 e-116 >gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 681 Score = 1333 bits (3449), Expect = 0.0, Method: Compositional matrix adjust. Identities = 657/665 (98%), Positives = 658/665 (98%) Query: 1 MQVSKPESAQGKPLSYRPDIDGLRAIAIVSVVVFHAFPDAMPGGFIGVDIFFVISGFLIS 60 MQVSKPESAQGKPLSYRPDIDGLRAIAIVSVVVFHAFPDAMPGGFIGVDIFFVISGFLIS Sbjct: 17 MQVSKPESAQGKPLSYRPDIDGLRAIAIVSVVVFHAFPDAMPGGFIGVDIFFVISGFLIS 76 Query: 61 SIIFRQLDQGRFSFTDFYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGA 120 SIIFRQLDQGRFSFTDFYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGA Sbjct: 77 SIIFRQLDQGRFSFTDFYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGA 136 Query: 121 GFIQNLVLWKESGYFDVESDMKPLLHLWSLSVEEQFYLVYPLLVCGLWRARMPLRKCLVL 180 GFIQNLVLWKESGYFDVESDMKPLLHLWSLSVEEQFYLVYPLLVCGLWRARMPLR LVL Sbjct: 137 GFIQNLVLWKESGYFDVESDMKPLLHLWSLSVEEQFYLVYPLLVCGLWRARMPLRNSLVL 196 Query: 181 LMLASFALNLWWVAQDPVKTFYLPFTRVWELLAGALCAFGVMRKGVAFSTSSFRLPHLPS 240 LMLASFALNLWWVAQDPVKTFYLPFTRVWELLAGALCAFGVMRKGVAFSTSSFRLPHLPS Sbjct: 197 LMLASFALNLWWVAQDPVKTFYLPFTRVWELLAGALCAFGVMRKGVAFSTSSFRLPHLPS 256 Query: 241 IHLRSVLGLALLLCGLVLIDRHRYFPGAWALLPVTGAVLLVSSGPQAWVNRHLLSRPLLV 300 IHLRSVLGLALLLCGLVLIDRHRYFPGAWALLPVTGAVLLVSSGPQAWVNRHLLSRPLLV Sbjct: 257 IHLRSVLGLALLLCGLVLIDRHRYFPGAWALLPVTGAVLLVSSGPQAWVNRHLLSRPLLV 316 Query: 301 WIGLVSYPLYLWHWPILSFMHILTPTRPGAPLRLAAVALSVLLAWLTYRLLERPVRFGAK 360 WIGLVSY LYLWHWPILSFMHILTPTRPGAPL LAAVALSVLLAWLTYRLLERPVRFGAK Sbjct: 317 WIGLVSYLLYLWHWPILSFMHILTPTRPGAPLPLAAVALSVLLAWLTYRLLERPVRFGAK 376 Query: 361 SWMVPALLTLLLSVTATAGYNDFQRNGLSFRLATHYAEQPLFVGTTDATCTADGGAGLEF 420 SWMVPALL LLLSVTATAGYNDFQRNGLSFRLATHYAEQPLFVGTTDATCTADGGAGLEF Sbjct: 377 SWMVPALLALLLSVTATAGYNDFQRNGLSFRLATHYAEQPLFVGTTDATCTADGGAGLEF 436 Query: 421 CRQSRPGAPTIVVIGDSHAWHLYDGLASYAAQSGDIIGMRAVGACLGFSGVSADTKRHDC 480 CRQSRPGAPTIVVIGDSHAWHLYDGLASYAAQ+GDIIGMRAVGACLGFSGVSADTKRHDC Sbjct: 437 CRQSRPGAPTIVVIGDSHAWHLYDGLASYAAQNGDIIGMRAVGACLGFSGVSADTKRHDC 496 Query: 481 APARDSIASAARQTSVRTVVLSARLDIYDRELPGEVFEGALRDMLATLTAAGKKVIFVMD 540 APARDSIASAARQ SVRTVVLSARLDIYDRELPGEVFEGALRDMLATLTAAGKKVIFVMD Sbjct: 497 APARDSIASAARQASVRTVVLSARLDIYDRELPGEVFEGALRDMLATLTAAGKKVIFVMD 556 Query: 541 IPILDFHPKYCLDISMPLRPKTTSLPNCAIQQAVFAQHTQHYRETVNALLRQFPQVQVFD 600 IPILDFHPKYCLDISMPLRPKTTSLPNCAIQQAVFAQHTQHYRETVNALLRQFPQVQ FD Sbjct: 557 IPILDFHPKYCLDISMPLRPKTTSLPNCAIQQAVFAQHTQHYRETVNALLRQFPQVQAFD 616 Query: 601 AAAPLCDHQQCWAMKDGQLLYRDYDHLSTIGSTLVAQTLWPLIHPIPQPGLRAAATKASP 660 AAAPLCDHQQCWAMKDGQLLYRDYDHLSTIGSTLVAQTLWPLIHPIPQPGLRAAATKASP Sbjct: 617 AAAPLCDHQQCWAMKDGQLLYRDYDHLSTIGSTLVAQTLWPLIHPIPQPGLRAAATKASP 676 Query: 661 ARKRS 665 ARKRS Sbjct: 677 ARKRS 681 >gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 680 Score = 451 bits (1159), Expect = e-127, Method: Compositional matrix adjust. Identities = 253/653 (38%), Positives = 370/653 (56%), Gaps = 44/653 (6%) Query: 15 SYRPDIDGLRAIAIVSVVVFHAFPDAMPGGFIGVDIFFVISGFLISSIIFRQLDQGRFSF 74 YR DIDGLRAIA+++VV FH FP+ + GGFIGVD+FFVISG+LIS+IIF LD+G F+F Sbjct: 13 KYRSDIDGLRAIAVLAVVSFHGFPNFVRGGFIGVDVFFVISGYLISTIIFENLDRGTFTF 72 Query: 75 TDFYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGAGFIQNLVLWKESGY 134 T+FY R+RRIFPAL+LVL++CFV+GWF LL +EY+QLGKH+A GAGF+ N +L ESGY Sbjct: 73 TEFYARRVRRIFPALLLVLSTCFVVGWFVLLTDEYKQLGKHIAGGAGFVSNFLLRDESGY 132 Query: 135 FDVESDMKPLLHLWSLSVEEQFYLVYPLLVCGLWRARMPLRKCLVLLMLASFALNLWWVA 194 FD ++ KPLLHLWSL +EEQFY+++P+L+ WR + L +++ LASFA N+ + Sbjct: 133 FDNSAETKPLLHLWSLGIEEQFYILWPMLLFFSWRRKFNLLTITIIVALASFAANIKGIN 192 Query: 195 QDPVKTFYLPFTRVWELLAGALCAFGVMRKGVAFSTSSFRLPHLPS-------------- 240 +D FYLP +R WELL+G L A+ + K +T R P L + Sbjct: 193 KDATAAFYLPQSRFWELLSGTLLAWFTLYK--QHTTIELR-PRLGTWILQLIYSEKRKSD 249 Query: 241 ----IHLRSVLGLALLLCGLVLIDRHRYFPGAWALLPVTGAVLLVSSGPQAWVNRHLLSR 296 I + S+ GL ++L G I++ FPG WAL+P+ G +L++++GP AWVNR LLS Sbjct: 250 GKFLIDVSSITGLCIVLYGFWRINKELAFPGVWALVPILGTILIIAAGPAAWVNRTLLSN 309 Query: 297 PLLVWIGLVSYPLYLWHWPILSFMHILTPTRPGAPLRLAAVALSVLLAWLTYRLLERPVR 356 +LVW GL+S+PLYLWHWP+LSF I+ P PLR+ A+AL+++LAWLTY ++ERP+R Sbjct: 310 KILVWFGLISFPLYLWHWPVLSFARIVENELPSYPLRVGAIALAIILAWLTYEIVERPIR 369 Query: 357 FG--AKSWMVPALLTLLLSVTATAGYNDFQRNGLSFRLATHYAEQPLFVGTTD--ATCTA 412 G A + + L L+++ GY F NG R++ G TA Sbjct: 370 TGNNANTKITVTALIGLMALVGAVGYRTFAANGWESRMSPDITRISRAAGEWQYPGKLTA 429 Query: 413 DGGAGLEFCRQSRPGAPTIVVIGDSHAWHLY---DGLASYAAQSGDIIGMRAVGACLGFS 469 F RQ+ + IGDS+ Y D L S + + + G CL Sbjct: 430 FNFENRTFYRQNSNMDEITLFIGDSNIEQYYVRTDELISTKPNNSNSVVFATGGGCLAIP 489 Query: 470 GVSADTKRHDCAPARDSIASAAR-QTSVRTVVLSARLDIYDRE---------LPGEVFEG 519 G D CA ++ + A+ SV +VV+ A+ + Y + G+ + Sbjct: 490 GSPYDEAHMSCANLMETALNYAKTNASVASVVIGAQWNSYLSDGYGLRGSFGTAGKDYSA 549 Query: 520 ALRDM---LATLTAAGKKVIFVMDIPILD-FHPKYCLDISMPLRPKTTSLPNCAIQQAVF 575 AL + + L GKKV F+++IPI D PK+ + ++ P +L + + Sbjct: 550 ALNRLARYIRELKYLGKKVYFILNIPIGDELDPKFMITRNLKHFPHVINLKERGLNRKDI 609 Query: 576 AQHTQHYRETVNALLRQFPQVQVFDAAAPLCDHQQCWAMKD-GQLLYRDYDHL 627 + ++ + + R V + LC+ +C ++ D G+ +Y+D HL Sbjct: 610 DERYGKIQKDLEQIARA-AGADVVNPMDFLCNATKCSSLDDEGEPIYKDRSHL 661 >gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 662 Score = 419 bits (1078), Expect = e-118, Method: Compositional matrix adjust. Identities = 261/652 (40%), Positives = 359/652 (55%), Gaps = 53/652 (8%) Query: 15 SYRPDIDGLRAIAIVSVVVFHAFPDAMPGGFIGVDIFFVISGFLISSIIFRQLDQGRFSF 74 +YRPDIDGLRA+A+ SVV+FHAFP+ GGFIGVDIFFVISGFLIS I+F+ L+ GRFS Sbjct: 18 AYRPDIDGLRAVAVGSVVIFHAFPEWFAGGFIGVDIFFVISGFLISLILFKNLEHGRFSI 77 Query: 75 TDFYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGAGFIQNLVLWKESGY 134 DFYV R+RRIFPAL+ V++ C V GWF L +EY+QLGKH+ G+ FI N + W+ESGY Sbjct: 78 VDFYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKHLFGGSTFISNFLFWRESGY 137 Query: 135 FDVESDMKPLLHLWSLSVEEQFYLVYPLLVCGLWRARMPLRKCLVLLMLASFALNLWWVA 194 FD ++ KPLLHLWSL++EEQFYL +PLL+ +W+ R + ++ L SF +NL+++ Sbjct: 138 FDNSAETKPLLHLWSLAIEEQFYLFWPLLLAFVWKRRWGFLGIMGVIGLLSFGVNLYFIG 197 Query: 195 QDPVKTFYLPFTRVWELL-AGALCAFGVMRKGVAFSTSSFRLPHLPSIHLRSVLGLALLL 253 D FY P R WEL+ GAL + R+ + T ++ +L++ LG ALL+ Sbjct: 198 HDQSAAFYSPLARFWELMIGGALAYINLHRQDL---TRGYK-------NLQAWLGAALLV 247 Query: 254 CGLVLIDRHRYFPGAWALLPVTGAVLLVSSGPQAWVNRHLLSRPLLVWIGLVSYPLYLWH 313 G L+D R FPG WALLP GA LL+S+GP AW NR +L+ +V +GLVSYPLYLWH Sbjct: 248 AGFALLDGARAFPGWWALLPSLGAFLLLSAGPGAWFNRRVLASKGMVALGLVSYPLYLWH 307 Query: 314 WPILSFMHILTPTRPGAPLRLAAVALSVLLAWLTYRLLERPVRFGAKSWMVPALLTLLLS 373 WP+L+F I+ A R AAV ++LL+WLTYR +ERP+R +S V LL + ++ Sbjct: 308 WPLLAFATIIEGESAPAEWRAAAVLGALLLSWLTYRYIERPLRGSTRSRTVLVLLAINVA 367 Query: 374 VTATAGYNDFQRNGLSFRLAT----HYAEQPLFVGT--TDATCTA-DGGAGL--EFCRQS 424 V G + + G R + + +F G+ +D +C +G G+ E C S Sbjct: 368 V-GLGGLAIYLKGGFDERAINGKLDYLTQSDVFEGSRNSDGSCARFNGMEGVDEEVC-LS 425 Query: 425 RPGAPTIVVIGDSHAWHLYDGLASYAAQSGDI---IGMRAVGACLGFSGVSADTKR---- 477 AP + +GDSH L+ A SGD+ M A AC + + Sbjct: 426 NSAAPRTLFVGDSHVMALHS-----AIFSGDVKMPAMMVAGHACRVYPNLPYTPTHLLRW 480 Query: 478 -HDC-APARDSIASAARQTSVRTVVLSARLDIYDRELPG------------EVFEGALRD 523 ++C A A ++IA A + S+ TVV+S D P E F Sbjct: 481 ANNCTAIANEAIAVAGKLPSIDTVVISGAYPRGDLSQPSEYQQDGRALSKREAFLSGYGA 540 Query: 524 MLATLTAAGKKVIFVMDIPILDFHPKYCLDISMPLRPKTTSLPNCAIQQAVFAQHTQHYR 583 ++ GKKV+F+ D+P L P C++ R +CA +A + Y Sbjct: 541 LIEAFRKQGKKVVFMPDVPYLKLDPHACVN-----RFSDGQARDCAYTEAEHQALRRDYD 595 Query: 584 ETVNALLRQFPQVQVFDAAAPLCDHQQCWAMKDGQLLYRDYDHLSTIGSTLV 635 V AL R P FD C +C A LY D H+S S L+ Sbjct: 596 AAVAALQRAHPDAVFFDPQGLFCPDGKCQAKLGEAYLYNDNQHISLAASRLL 647 >gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum seropedicae SmR1] Length = 656 Score = 412 bits (1058), Expect = e-116, Method: Compositional matrix adjust. Identities = 279/640 (43%), Positives = 376/640 (58%), Gaps = 37/640 (5%) Query: 15 SYRPDIDGLRAIAIVSVVVFHAFPDAMPGGFIGVDIFFVISGFLISSIIFRQLDQGRFSF 74 YRPDIDGLRAIA++SVVVFHAFP+ +PGGF+GVDIFFVISGFLIS I+F L + RFS Sbjct: 21 GYRPDIDGLRAIAVLSVVVFHAFPEWLPGGFVGVDIFFVISGFLISGILFDALQRDRFSI 80 Query: 75 TDFYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGAGFIQNLVLWKESGY 134 DFY+ RIRRIFPAL+LV+ +C + GWF LLPEEYRQLGKH+ GAGF+ N+VLW E+GY Sbjct: 81 VDFYIRRIRRIFPALLLVMLACLLAGWFILLPEEYRQLGKHLLGGAGFVSNIVLWNEAGY 140 Query: 135 FDVESDMKPLLHLWSLSVEEQFYLVYPLLVCGLWRARMPLRKCLVLLMLASFALNLWWVA 194 FD ++ KPLLHLWSL VEEQFYL +PL++ WR R + L+ +M SF N+ W+ Sbjct: 141 FDTSAETKPLLHLWSLGVEEQFYLFWPLMLWAAWRRRTGMLAVLLGVMALSFGWNIVWLH 200 Query: 195 QDPVKTFYLPFTRVWELLAGALCAFGVMRKGVAFSTSSFRLPHLPSIHLRSVLGLALLLC 254 +D V FYLP +R WEL AGAL A+ + V S + RL SV GL L++ Sbjct: 201 RDAVAAFYLPMSRFWELGAGALLAWRHRQGAVPTSVQAHRL---------SVAGLLLIVV 251 Query: 255 GLVLIDRHRYFPGAWALLPVTGAVLLVSSGPQAWVNRHLLSRPLLVWIGLVSYPLYLWHW 314 + I + FPG WALLPVTGAVLL+++GPQA +NR LLS +VW+GL+SYPLYLWHW Sbjct: 252 AIATITKDDAFPGWWALLPVTGAVLLLAAGPQARINRSLLSHRTMVWVGLISYPLYLWHW 311 Query: 315 PILSFMHILTPTRPGAPLRLAAVALSVLLAWLTYRLLERPVRFGAKSWMVPALLTLLLSV 374 P+L+F ++ P LRLAAVAL++LLAWLTYRL+E PVR + L LL+++ Sbjct: 312 PLLAFARLVVGATPSVQLRLAAVALALLLAWLTYRLVELPVRLRSAGHRSAVTLALLMAL 371 Query: 375 TATAGYNDFQRNGLSFR----LATHYAEQPLFVGTTD------ATCTADGGA--GLEFCR 422 A G +RNG+ FR +A + +Q + +C A G ++C Sbjct: 372 MAALGGYVVKRNGMDFRQLGAVAMLFNDQVKETAVLNHFELPHPSCAALMGQEHSRDWCA 431 Query: 423 QSRPGA-PTIVVIGDSHAWHLYDGLAS-YAA--QSGDIIGMRAVGACLGFSGVSADTKRH 478 P +++IGDS++ LA Y A Q + A G C D Sbjct: 432 APVSAEPPQVLLIGDSYSGAYAPMLARLYQASPQPQLVFQQFARGGCASL----LDGGGG 487 Query: 479 DCAPARDSIASAARQT-SVRTVVLSARLDIYDRELPGEVFEGALRDMLATLTAAGKKVIF 537 C + +A+ ARQT S++TVVL+A + P V + AL + GK+V+ Sbjct: 488 YCGEISERVAAYARQTPSIKTVVLAANWPGHIAANPKGVLK-ALEHTILYYRQLGKRVVV 546 Query: 538 VMDIPILDFHPKYCLDISMPLRPKTTSLPNCAIQQAVFAQHTQHYRETVNALLRQFPQVQ 597 ++ P +PK C+ + + + C + +A Q HYR+ + LL++ V Sbjct: 547 LLSPPN-GANPKSCVLRGI----RLSDADFCNLPRAKAEQMDDHYRDLMLPLLQRL-DVP 600 Query: 598 VFDAAAPLCDHQQCWAMKDGQLLYRDYDHLSTIGSTLVAQ 637 VFD LCD C + ++LY D HLS G+ +A Sbjct: 601 VFDPYPTLCDEHGCKVIDGPRILYFDPGHLSAYGAQYLAD 640 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.450 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,018,868 Number of extensions: 80652 Number of successful extensions: 352 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 338 Number of HSP's successfully gapped: 6 Length of query: 665 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 574 Effective length of database: 1,188,985 Effective search space: 682477390 Effective search space used: 682477390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 134 (56.2 bits)