BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0170.p (328 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 659 0.0 gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 145 4e-36 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 139 3e-34 gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 109 3e-25 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 107 2e-24 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 659 bits (1700), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/328 (99%), Positives = 328/328 (100%) Query: 1 MHRRDFLRLAPAALGVTALGGIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK 60 MHRRDFLRLAPAALGVTALGGIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK Sbjct: 1 MHRRDFLRLAPAALGVTALGGIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK 60 Query: 61 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYSRPEWTAL 120 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYSRPEWTAL Sbjct: 61 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYSRPEWTAL 120 Query: 121 AVGKESPVKSISELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALE 180 AVGKESPVKSI+ELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALE Sbjct: 121 AVGKESPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALE 180 Query: 181 QGRVAAWAGLDPHLAASELEAGSRLIYRNVNFNTYGFLNVSDSFAARHPDQVKRVIAAYE 240 QGRVAAWAGLDPHLAASELEAGSRLIYRNVNFNTYGFLNVSDSFAARHPDQVKRVIAAYE Sbjct: 181 QGRVAAWAGLDPHLAASELEAGSRLIYRNVNFNTYGFLNVSDSFAARHPDQVKRVIAAYE 240 Query: 241 RARSWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSQPQIGREHIEALRAAAPILLEE 300 RARSWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSQPQIGREHIEALRAAAPILLEE Sbjct: 241 RARSWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSQPQIGREHIEALRAAAPILLEE 300 Query: 301 DLVKSGTDLPRTINALIDPSYAQGVVRA 328 DLVKSGTDLPRTINALIDPSYAQGVVRA Sbjct: 301 DLVKSGTDLPRTINALIDPSYAQGVVRA 328 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 145 bits (366), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 102/276 (36%), Positives = 145/276 (52%), Gaps = 7/276 (2%) Query: 3 RRDFLRLAPAALG--VTALGGIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK 60 RR RL+ ALG ALG P+ AQ K++R+ Y + ++L+ G LE+ L+ Sbjct: 14 RRAIQRLSAIALGALTIALGASPV---QAQETKLLRIGYQKAANTLVLLKAHGTLEKRLQ 70 Query: 61 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANG-NPVKAVYIYSRPEWTA 119 G EVKW + LE LN SVDFG + A+A G + V Y P+ Sbjct: 71 PLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPDAEG 130 Query: 120 LAVGKESPVKSISELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAAL 179 L V K SP+KS++ELKGKKIA KG+D + ++ L + GL+ SD++ V+L AD RAA Sbjct: 131 LLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPADARAAF 190 Query: 180 EQGRVAAWAGLDPHLAASELEAGSRLIYRNVN-FNTYGFLNVSDSFAARHPDQVKRVIAA 238 E+G V AWA DP AA+E + G+R + V N + F + FAA++P K ++ Sbjct: 191 ERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKNPQVEKILLEE 250 Query: 239 YERARSWIIAHPDETVALLSEESKLSAPVARLQLKR 274 R W+ H + A L+ L A + LKR Sbjct: 251 ISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKR 286 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 139 bits (350), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 102/310 (32%), Positives = 160/310 (51%), Gaps = 14/310 (4%) Query: 2 HRRDFLRLAPAALGVTALG-GIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK 60 H+R RL L AL G PL+ +AA KV+R+ Y ++ + +L+ G LE+ L+ Sbjct: 8 HKR---RLLLQGLAAGALASGTPLVRAAAD--KVLRIGYQKFNTLN-ILKGSGKLEQALQ 61 Query: 61 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYSR---PEW 117 QG VKW + E LN++++D G A ++ A A+G V AVY+ + P+ Sbjct: 62 PQGWSVKWFEFAAGPQLTEALNANAIDLGHAADTPSVFANASG--VNAVYLGAEQPYPKG 119 Query: 118 TALAVGKESPVKSISELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQH-ADGR 176 + V +ESP++S+++LKGKK+A +G + LL+AL E GL D+E V++ + AD R Sbjct: 120 IGIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVR 179 Query: 177 AALEQGRVAAWAGLDPHLAASELEAGSRLIYRNVNF-NTYGFLNVSDSFAARHPDQVKRV 235 AA + G V A DP LA EL R++ N F SF RH D V+ V Sbjct: 180 AAFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTV 239 Query: 236 IAAYERARSWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSQPQIGREHIEALRAAAP 295 A ++ W +HP ET LL+ + + V RL +R +++ + + + A Sbjct: 240 FAELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQIAD 299 Query: 296 ILLEEDLVKS 305 L+ L+K+ Sbjct: 300 AFLKLGLIKN 309 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 109 bits (272), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 86/274 (31%), Positives = 136/274 (49%), Gaps = 14/274 (5%) Query: 48 VLRKFGWLEEDLKAQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVK 107 ++R G LE+ L G VKWV LE L + S+DFG + A+A G P+ Sbjct: 64 LIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGTPL- 122 Query: 108 AVYI---YSRPEWTALAVGKESPVKSISELKGKKIAATKGTDPYLFLLRALHENGLKKSD 164 VY+ RP A+ V +S ++ +LKGK+IA KG++ + FL++ L ++GLK D Sbjct: 123 -VYVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYGD 181 Query: 165 VEIVHLQHADGRAALEQGRVAAWAGLDPHLAASELEAGSRLI--YRNVNFNTYGFLNVSD 222 V V L AD RAA E+G V AWA +P+LAA++ +R++ V N + + D Sbjct: 182 VTPVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTRD 241 Query: 223 SFAARHPDQVKRVIAAYERARSWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSQPQI 282 +A H D ++ + + +W A PDE + L+ L V + ++R + I Sbjct: 242 -YARDHADLLRIALEELGKIDAWAQARPDEYASELAGILGLPRSVLDVVVRRYHYGTLPI 300 Query: 283 GREHIEALRAAAPILLEEDLVKSGTDLPRTINAL 316 R ++ + A L+ L+ PR I+ L Sbjct: 301 TRAILDEQQQIADTFLDLRLI------PRKISLL 328 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 99/309 (32%), Positives = 137/309 (44%), Gaps = 22/309 (7%) Query: 17 TALGGIPLLSSA---AQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLKAQGTEVKWVLSQG 73 TALG L+ A AQ K IR+ Y S +L+ G LE+ L G ++ W Sbjct: 18 TALGLGSALAPASVFAQGNKAIRIGYQKSSTLLTILKANGTLEKLLAPTGAKISWHEFAS 77 Query: 74 SNRALEYLNSDSVDFGS-TAGLAALLARANGNPVKAVYIYS-RPEWTALAVGKESPVKSI 131 LE LN VD + A + A+A G + V S P A+ V +S ++ Sbjct: 78 GLPLLEALNVGGVDLSADVADTVPVFAQAAGAKLAYVAQESPSPAAQAIVVRADSVLRKA 137 Query: 132 SELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALEQGRVAAWAGLD 191 S+LKGKKIA TK + L+ L + GLK SDV+ +L ADGRAA E+G V AW D Sbjct: 138 SDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDAAYLSPADGRAAFERGSVDAWVTWD 197 Query: 192 PHLAASELEAGSRLIYRNVNFNTYG-FLNVSDSFAARHPDQVKRVIAAYERARSWIIAHP 250 P LA + ++ R++ Y + S SFA P+ ++ V ++ W+ HP Sbjct: 198 PFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQARPEVLRVVFDELQKTGHWVKQHP 257 Query: 251 DETVALLSEESKLSAPVARLQLKRNDFSQPQIGREHIEALRAAAPILLEE-----DLVKS 305 E A L+ L A L R + +RA P L E D + Sbjct: 258 KEAAAFLAPLWGLDADTIELANSRRSYE-----------VRAVRPEALGEQQRIADTFFA 306 Query: 306 GTDLPRTIN 314 LPR IN Sbjct: 307 AGLLPRKIN 315 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,020,088 Number of extensions: 40817 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 7 Length of query: 328 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 242 Effective length of database: 1,212,660 Effective search space: 293463720 Effective search space used: 293463720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)