BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0156.p (408 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309974|ref|YP_003774066.1| poly-beta-hydroxyalkanoate depo... 842 0.0 gi|300310951|ref|YP_003775043.1| poly[D(-)-3-hydroxyalkanoate] d... 366 e-102 gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthet... 77 2e-15 >gi|300309974|ref|YP_003774066.1| poly-beta-hydroxyalkanoate depolymerase [Herbaspirillum seropedicae SmR1] Length = 408 Score = 842 bits (2174), Expect = 0.0, Method: Compositional matrix adjust. Identities = 406/408 (99%), Positives = 407/408 (99%) Query: 1 MIPTYQLYQNYADATDPLRACARMMAQALGATWPGIPVHPYWRKMASACEVFARTQLTHA 60 MIPTYQLYQNYADATDPLRACARMMAQALGATWPGIPVHPYWRKMASACEVFARTQLTHA Sbjct: 1 MIPTYQLYQNYADATDPLRACARMMAQALGATWPGIPVHPYWRKMASACEVFARTQLTHA 60 Query: 61 RPPFGIATVEEEGRTISVHEEEIHATPFCGLLHFRKNSATVQPKVLVVAPMSGHFATLLR 120 RPPFGIATVEEEG TISVHEEEIHATPFCGLLHFRK+SATVQPKVLVVAPMSGHFATLLR Sbjct: 61 RPPFGIATVEEEGCTISVHEEEIHATPFCGLLHFRKDSATVQPKVLVVAPMSGHFATLLR 120 Query: 121 GTVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGVLGPGAHLLAVCQPTV 180 GTVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGVLGPGAHLLAVCQPTV Sbjct: 121 GTVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGVLGPGAHLLAVCQPTV 180 Query: 181 AALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKPIAWFEKNMISTVPAR 240 AALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKPIAWFEKNMISTVPAR Sbjct: 181 AALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKPIAWFEKNMISTVPAR 240 Query: 241 HAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARADQIKDFYEEYFAMAD 300 HAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARADQIKDFYEEYFAMAD Sbjct: 241 HAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARADQIKDFYEEYFAMAD 300 Query: 301 LPAEFYLETVRTVFQEHALPLGKLSYAGRPVEPRAIRRTALFTIEGEKDDICAVGQTLAA 360 LPAEFYLETVRTVFQEHALPLGKLSYAGRPVEPRAIRRTALFTIEGEKDDICAVGQTLAA Sbjct: 301 LPAEFYLETVRTVFQEHALPLGKLSYAGRPVEPRAIRRTALFTIEGEKDDICAVGQTLAA 360 Query: 361 QELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHHR 408 QELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHHR Sbjct: 361 QELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHHR 408 >gi|300310951|ref|YP_003775043.1| poly[D(-)-3-hydroxyalkanoate] depolymerase [Herbaspirillum seropedicae SmR1] Length = 411 Score = 366 bits (940), Expect = e-102, Method: Compositional matrix adjust. Identities = 185/405 (45%), Positives = 255/405 (62%), Gaps = 6/405 (1%) Query: 5 YQLYQNYADATDPLRACARMMAQALGATWPGIPVHPYWRKMASACEVFARTQLTHARPPF 64 YQL++ +P+ A A+ + P+ +++A+ E+ R + +P F Sbjct: 3 YQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKPQF 62 Query: 65 GIATVEEEGRTISVHEEEIHATPFCGLLHFRKN------SATVQPKVLVVAPMSGHFATL 118 I V +G+++ + EE + PFC L+HFRK+ +A QP VLVVAP+SGH +TL Sbjct: 63 NIDAVPVDGKSVGIVEEVVEEKPFCRLIHFRKDLSARQATALKQPTVLVVAPLSGHHSTL 122 Query: 119 LRGTVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGVLGPGAHLLAVCQP 178 LR TVR LL++HDVYITDW +AR V G F L +YV ++ DF+ LGP H+++VCQP Sbjct: 123 LRETVRALLQEHDVYITDWTDARMVPVEQGAFHLHDYVYYVQDFIRTLGPDVHVISVCQP 182 Query: 179 TVAALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKPIAWFEKNMISTVP 238 TV L A ++MA+ P P+SMT+M GPID R +PTAVN LA KP +WFE +I +VP Sbjct: 183 TVPVLAAISLMASANDPMLPKSMTMMGGPIDARKSPTAVNDLATEKPYSWFENTVIYSVP 242 Query: 239 ARHAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARADQIKDFYEEYFAM 298 A + G GR+VYPGF+Q A F+ MN RH ++ Y HL DG+ A A++ + FY+EY A+ Sbjct: 243 ANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYNAV 302 Query: 299 ADLPAEFYLETVRTVFQEHALPLGKLSYAGRPVEPRAIRRTALFTIEGEKDDICAVGQTL 358 D+PAEFYLET++ VFQ+ L G G+ V P+ I+ ALFTIEGE DDI GQT Sbjct: 303 LDMPAEFYLETIKVVFQDFNLARGTWEIEGQLVRPQDIKSVALFTIEGELDDISGSGQTQ 362 Query: 359 AAQELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFI 403 AAQELCS I + H GHYG+F+GRRW + P++ +FI Sbjct: 363 AAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFI 407 >gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 77.4 bits (189), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 92/384 (23%), Positives = 161/384 (41%), Gaps = 49/384 (12%) Query: 40 PYWRKMASACEVFARTQLTHARPPFGIATVEEEGRTISVHEEEIHATP---------FCG 90 P + MA A + F + Q + + +EEE + +VH + ATP Sbjct: 18 PLFWPMAFAAQAFKQGQDMMDKQ---LRFLEEEAKLHAVHHPRM-ATPHRERLRLRTLTL 73 Query: 91 LLHFRKNSATVQPKVLVVAPMSGHFATLL-----RGTVRTLLR---DHDVYITDWRNARD 142 + ++ L+VAP +GH + + + V TLL DH V + DW +A Sbjct: 74 REYGSADAGHATVPTLIVAPYAGHTSVIADYDQGQSLVETLLGNGVDH-VLLIDWHSA-- 130 Query: 143 VATAHGRFGLDEYVSHIIDFLGVLGPGAHLLAVCQPT-VAALTAAAVMAADGHPAQPRSM 201 + +D Y++ + + LG L+ +CQ ++A+ AA P + + Sbjct: 131 -SADMKDLEIDNYLADLCVVIDELGGRVRLVGLCQGGWISAMIAARF------PEKVARL 183 Query: 202 TLMAGPIDTRVNPTAVNALAKSKPIAWFEKNMISTVPARHAGAGRRVYPGFVQLAAFMNM 261 L PID + + + P+ ++E + R G R G L + NM Sbjct: 184 VLAGSPIDAGAGEGPLKQMVQRLPLRFYEDLV------RSGGGLMR---GSFMLQGWKNM 234 Query: 262 NLSRH-VEAFGKLYHHLVDGEH-ARADQIKDFYEEYFAMADLPAEFYLETVRTVFQEHAL 319 + H +L H+ D + A+ + +YE DLP +YL+ + +F+++ L Sbjct: 235 HPDEHYFNEHLELLQHIDDPAYLAKRETFAAWYE---TTVDLPGRWYLQAIGHIFKDNLL 291 Query: 320 PLGKLSYAGRPVEPRAIRRTALFTIEGEKDDICAVGQTLAAQELCSGIRPYMRLHHVQTA 379 GK G+ ++ R I L+ + GE+DDI Q L A L P ++ Sbjct: 292 ARGKYVALGQQLDLRQI-TCPLYLLAGERDDITTPEQVLNAAALVG--TPAAQITQKIVP 348 Query: 380 VGHYGVFNGRRWDNEIYPRLRDFI 403 GH G+F G R E++P++ ++ Sbjct: 349 GGHIGLFMGSRTLAEVWPQIATWL 372 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.137 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,291,689 Number of extensions: 51917 Number of successful extensions: 208 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 205 Number of HSP's successfully gapped: 3 Length of query: 408 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 321 Effective length of database: 1,207,925 Effective search space: 387743925 Effective search space used: 387743925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)