BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0143.p (389 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 750 0.0 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 274 7e-75 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 181 8e-47 gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 146 2e-36 gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 88 1e-18 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 750 bits (1936), Expect = 0.0, Method: Compositional matrix adjust. Identities = 376/376 (100%), Positives = 376/376 (100%) Query: 1 MIRVMVVDDSSVVRQLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRM 60 MIRVMVVDDSSVVRQLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRM Sbjct: 6 MIRVMVVDDSSVVRQLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRM 65 Query: 61 DGVSFLRQIMATRPTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQQAVEE 120 DGVSFLRQIMATRPTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQQAVEE Sbjct: 66 DGVSFLRQIMATRPTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQQAVEE 125 Query: 121 LASAIRAAMQAREGQRAPKAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVLQPPGP 180 LASAIRAAMQAREGQRAPKAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVLQPPGP Sbjct: 126 LASAIRAAMQAREGQRAPKAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVLQPPGP 185 Query: 181 EDGLRPATARIVAIGASTGGPQALERVLGKLSQRCPGIVVVQHMPQRFTRSFAERLHRIS 240 EDGLRPATARIVAIGASTGGPQALERVLGKLSQRCPGIVVVQHMPQRFTRSFAERLHRIS Sbjct: 186 EDGLRPATARIVAIGASTGGPQALERVLGKLSQRCPGIVVVQHMPQRFTRSFAERLHRIS 245 Query: 241 GAEVKEAEHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSL 300 GAEVKEAEHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSL Sbjct: 246 GAEVKEAEHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSL 305 Query: 301 AKAAGSNAVGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDV 360 AKAAGSNAVGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDV Sbjct: 306 AKAAGSNAVGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDV 365 Query: 361 ITLEHISAVIEQYACR 376 ITLEHISAVIEQYACR Sbjct: 366 ITLEHISAVIEQYACR 381 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 274 bits (701), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 149/363 (41%), Positives = 225/363 (61%), Gaps = 26/363 (7%) Query: 2 IRVMVVDDSSVVRQLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRMD 61 I+V++VDDS+++R +M++++ PD+ V+G APDP+ A ++Q PDV+ LD++MPRMD Sbjct: 3 IKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMD 62 Query: 62 GVSFLRQIMATRPTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQQAVEEL 121 G+ FL ++M RP PV+M S+ + + AL GA+ + +P++ ++ +Q+ + + Sbjct: 63 GLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMI 122 Query: 122 ASAIRAAMQAREGQRAPKAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVLQPPGPE 181 A IRAA +AR V+A AA G ++A+ Q P Sbjct: 123 ADKIRAAAKAR----------------VRARPARSAEQAAGGG------SEALPQIRNP- 159 Query: 182 DGLRPATARIVAIGASTGGPQALERVLGKLSQRCPGIVVVQHMPQRFTRSFAERLHRISG 241 ++ +++ IGASTGG +A++ L ++ CPGI++ QHMP+ FTRSFA+RL+ + Sbjct: 160 ---LTSSEKLIIIGASTGGTEAIKEFLIRMPSDCPGILITQHMPEGFTRSFAQRLNNLCK 216 Query: 242 AEVKEAEHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSLA 301 V E+ +R+LPG IAPG HL + R G Y + GP V+RH+PSVDVLF+S A Sbjct: 217 ISVVESAGNERVLPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAA 276 Query: 302 KAAGSNAVGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDVI 361 AG NAVG+I+TGMG DGA+GM E+ + G +AQDEAS VVFGMP+EAI G +V+ Sbjct: 277 TCAGKNAVGVILTGMGKDGAQGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVL 336 Query: 362 TLE 364 L+ Sbjct: 337 ALQ 339 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 181 bits (460), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 137/379 (36%), Positives = 194/379 (51%), Gaps = 35/379 (9%) Query: 1 MIRVMVVDDSSVVRQLMQQVLAQTP--DIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMP 58 MI+++++DDS+++R+ + QV Q DI + + V R PDV+ LDI MP Sbjct: 1 MIKLLIIDDSALMRRQLMQVFEQAGGFDICLGRNGREAVELNRSFA---PDVVTLDINMP 57 Query: 59 RMDGVSFLRQIMATRPTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQQAV 118 MDG+S L +M RP PV+M S+ EA+ GAV +A+P + L + Sbjct: 58 EMDGLSALSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLHEIE 117 Query: 119 EELASAIRAAMQAREGQRAPKAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVLQPP 178 ++L + +R A +AR RAP +P A ARE L AA A R Sbjct: 118 QDLLAKVRVAAKARIRSRAP--------APELARAREPL--AAPVAEAARRG-------- 159 Query: 179 GPEDGLRPATARIVAIGASTGGPQALERVLGKLSQRCPG-IVVVQHMPQRFTRSFAERLH 237 RPA+ +V IG STGGP LE +L L P I+V QHMP FTRSFAER+ Sbjct: 160 ------RPASDGLVLIGVSTGGPSTLEEILPALPASFPWPILVAQHMPAAFTRSFAERMD 213 Query: 238 RISGAEVKEAEHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRH--KPSVDV 295 ++ V E + G V IA GG ++V + G ++ L P H PSV++ Sbjct: 214 KLCALNVLEVAAPTVVQGGHVYIARGGADMLVAKRG--SHLSVLPKPESPTHLWHPSVEL 271 Query: 296 LFRS-LAKAAGSNAVGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQ 354 L RS L A +G+++TGMG+DGA E+ GG T A+ E+S VVFGMPK+ I + Sbjct: 272 LARSALQSMAARQLIGVMLTGMGNDGAAAFAEICGDGGRTIAESESSCVVFGMPKDLIDR 331 Query: 355 GGVGDVITLEHISAVIEQY 373 GG V+ I+ + + Sbjct: 332 GGASVVLPASKIATQLRVW 350 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 146 bits (369), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 114/377 (30%), Positives = 189/377 (50%), Gaps = 46/377 (12%) Query: 2 IRVMVVDDSSVVRQLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRMD 61 +++ +V+D+ ++ +++++V+A++ +I A D A++ Q PDV+++D+ MP++D Sbjct: 1 MKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVD 60 Query: 62 GVSFLRQIMATRPTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQL----GVRDF--LQ 115 G R+IM P P+++ ++ + EA+ GA+ P L G RD L Sbjct: 61 GAEATRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRDAAALI 120 Query: 116 QAVEELASAIRAAMQAREGQRAPKAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVL 175 + ++ L G+ +P+ + PV+ G E L A Sbjct: 121 EKIDNL------------GRLSPEMVS----PPVRGGRAETLAPAQ-------------- 150 Query: 176 QPPGPEDGLRPATARIVAIGASTGGPQALERVLGKLSQRCPG-IVVVQHMPQRFTRSFAE 234 P D L P +VA+GAS GGP AL +L + Q G +V+VQH+ F A+ Sbjct: 151 --PARADSLPP----LVAVGASAGGPAALATLLKAIPQGFAGAVVIVQHIDAAFAPGMAQ 204 Query: 235 RLHRISGAEVKEAEHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVD 294 L + S +V A+ G VL+A RHLVV+ G YVE G ++PSVD Sbjct: 205 WLQQQSALKVGLAQEGQAPQRGAVLLAASNRHLVVKEGGRLGYVE---GAPNDVYRPSVD 261 Query: 295 VLFRSLAKAAGSNAVGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQ 354 V F+S+ + G+++TGMG DGA G++ L D G T AQD+ S V+GMPK A+ Sbjct: 262 VFFQSIVRHWPGKVAGLLLTGMGRDGAAGLKALRDSGAHTMAQDQKSCAVYGMPKAAVAL 321 Query: 355 GGVGDVITLEHISAVIE 371 +V+ + ++A ++ Sbjct: 322 NAAVEVLPVTGMAARLQ 338 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 87.8 bits (216), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 66/198 (33%), Positives = 100/198 (50%), Gaps = 11/198 (5%) Query: 178 PGPEDGLRPATARIVAIGASTGGPQALERVLGKLSQRCP-GIVVVQHMPQR----FTRSF 232 P PE RP A + IGAS GG +AL +L L + +VV H+P+ + F Sbjct: 5 PLPELAARPIEA--IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIF 62 Query: 233 AERLHRISGAEVKEAEHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPS 292 A+R + V EA+ + G + AP HL+V R + + LV+ +P+ Sbjct: 63 ADRCRQ----PVVEAQDKEPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPA 118 Query: 293 VDVLFRSLAKAAGSNAVGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAI 352 +DVLF S A G +GI++TG DGA GM+ +A GG T Q + V MP +A+ Sbjct: 119 IDVLFESAADVYGPRLLGILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQAL 178 Query: 353 QQGGVGDVITLEHISAVI 370 G + V+TL+ I+ ++ Sbjct: 179 AMGNIDYVLTLQQIADLL 196 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,268,092 Number of extensions: 57232 Number of successful extensions: 269 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 273 Number of HSP's successfully gapped: 11 Length of query: 389 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 302 Effective length of database: 1,207,925 Effective search space: 364793350 Effective search space used: 364793350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)