BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0142.p (277 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransf... 568 e-163 gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransf... 137 7e-34 gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransf... 136 2e-33 gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis... 85 7e-18 gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransfe... 68 9e-13 >gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 277 Score = 568 bits (1464), Expect = e-163, Method: Compositional matrix adjust. Identities = 277/277 (100%), Positives = 277/277 (100%) Query: 1 MTGPHGIALDEEVSDHDMLLFQRLFKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQY 60 MTGPHGIALDEEVSDHDMLLFQRLFKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQY Sbjct: 1 MTGPHGIALDEEVSDHDMLLFQRLFKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQY 60 Query: 61 HALISSEAGAQERQRAIDLITTNETYFFREPGHFDFVVGQWLPQLAQQPQLRIWSAASAT 120 HALISSEAGAQERQRAIDLITTNETYFFREPGHFDFVVGQWLPQLAQQPQLRIWSAASAT Sbjct: 61 HALISSEAGAQERQRAIDLITTNETYFFREPGHFDFVVGQWLPQLAQQPQLRIWSAASAT 120 Query: 121 GEEAYTLAMLLDHHRPGASWSVFASDISTRVLRFARRALYPMVRGQNIPRAYLRRYCLMG 180 GEEAYTLAMLLDHHRPGASWSVFASDISTRVLRFARRALYPMVRGQNIPRAYLRRYCLMG Sbjct: 121 GEEAYTLAMLLDHHRPGASWSVFASDISTRVLRFARRALYPMVRGQNIPRAYLRRYCLMG 180 Query: 181 VGEYEGHFLVQADLRAKVQFAQRNLTRLDEHDDGQYDLIFLRNVIIYFDCETKLQVLRDV 240 VGEYEGHFLVQADLRAKVQFAQRNLTRLDEHDDGQYDLIFLRNVIIYFDCETKLQVLRDV Sbjct: 181 VGEYEGHFLVQADLRAKVQFAQRNLTRLDEHDDGQYDLIFLRNVIIYFDCETKLQVLRDV 240 Query: 241 IARLRPGGYLVVGHSESLHGMDLGLDMQSPSIYRKPA 277 IARLRPGGYLVVGHSESLHGMDLGLDMQSPSIYRKPA Sbjct: 241 IARLRPGGYLVVGHSESLHGMDLGLDMQSPSIYRKPA 277 >gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 290 Score = 137 bits (346), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 85/252 (33%), Positives = 136/252 (53%), Gaps = 5/252 (1%) Query: 24 LFKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQYHALISSEAGAQERQRAIDLITTN 83 L Q+ G+ L SK+ ++ SRLA RL G+ QY ++ ++E + + +TTN Sbjct: 29 LIYQRAGIALADSKQEMVYSRLARRLRATGITSFQQYLDMLERTHDSEEWEAFTNALTTN 88 Query: 84 ETYFFREPGHFDFVVGQWLPQLAQQPQLRIWSAASATGEEAYTLAMLLDHHRPGASWSV- 142 T FFRE HF ++ +L L ++P IW +A++TGEE YT+AM + V Sbjct: 89 LTSFFREAHHFP-ILADFLKTL-KEPAT-IWCSAASTGEEPYTIAMTACEAYGSLTPPVQ 145 Query: 143 -FASDISTRVLRFARRALYPMVRGQNIPRAYLRRYCLMGVGEYEGHFLVQADLRAKVQFA 201 A+DI T VL +YPM R + + ++++ L G G +EG+ V+ +LR + F Sbjct: 146 IVATDIDTNVLATGSNGVYPMERIEKMSPERVKKFFLRGKGSHEGYVRVRPELRKLITFK 205 Query: 202 QRNLTRLDEHDDGQYDLIFLRNVIIYFDCETKLQVLRDVIARLRPGGYLVVGHSESLHGM 261 Q NL GQ+D+IF RNV+IYFD T+ ++L + ++P L GHSE+ + Sbjct: 206 QLNLLADGWPLSGQFDVIFCRNVMIYFDKPTQSKILSRFVPLMKPHALLFAGHSENFLYV 265 Query: 262 DLGLDMQSPSIY 273 L ++ ++Y Sbjct: 266 SDALKLKGKTVY 277 >gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 136 bits (343), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 85/254 (33%), Positives = 131/254 (51%), Gaps = 12/254 (4%) Query: 13 VSDHDMLLFQRLFKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQYHALISSEAGAQE 72 +S+ D L F+ F ++ G+ +K+ + RL R+ G Y +L+ EA +E Sbjct: 6 ISEADFLKFREYFYRKTGISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFEADGKE 65 Query: 73 RQRAIDLITTNETYFFREPGHFDFVVGQWLPQLAQQPQ----LRIWSAASATGEEAYTLA 128 Q +L+T NETYF RE F +V LP++ + + +RIW ++GEEAYTLA Sbjct: 66 LQLLTNLMTVNETYFLREEYQFQCLVNSMLPEITRNRRSTAPIRIWCIPCSSGEEAYTLA 125 Query: 129 MLLDHHRPGAS-WSV--FASDISTRVLRFARRALYPMVRGQNIPRAYLRRYCLMGVGEYE 185 M L PG W V +SDI T VLR A+ Y Q++P ++ +Y L EY+ Sbjct: 126 MYLMERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLRQGDEYQ 185 Query: 186 GHFLVQADLRAKVQFAQRNLTRL-DEHDDGQYDLIFLRNVIIYFDCETKLQVLRDVIARL 244 + LR V+F + NL + D +D+IF RN++IYFD ++ + L Sbjct: 186 ----ICDALRGAVEFTRVNLAKSQDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEAL 241 Query: 245 RPGGYLVVGHSESL 258 G++ +GHSES+ Sbjct: 242 NLNGFICLGHSESM 255 >gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis proteins protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 84.7 bits (208), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 73/205 (35%), Positives = 106/205 (51%), Gaps = 14/205 (6%) Query: 74 QRAIDLITTNETYFFREPGHFDFVVGQWLPQLAQQPQ--LRIWSAASATGEEAYTLAM-L 130 Q I+L ET+FFR+ V +LA QP +RI S +TGEE Y+LAM L Sbjct: 58 QALIELAVVPETWFFRDREAILAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAMAL 117 Query: 131 LDHHRPGASWSVFASDISTRVLRFARRALY--PMVRGQNIPRAYLRRYCLMGVGEYEGHF 188 LD + + A DI TR L A LY RGQ + A+ R+ + G++ H Sbjct: 118 LDAGLTPGQFVIDAYDIRTRSLEIAAAGLYGRNSFRGQEL--AFRDRHFDLEQGQWRLH- 174 Query: 189 LVQADLRAKVQFAQRNLTRLDEHDDGQ-YDLIFLRNVIIYFDCETKLQVLRDVIARLRPG 247 +R++V+FA NL D Q YD IF RNV+IYF+ + + QV++ + + ++P Sbjct: 175 ---ERIRSQVRFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQRQVVQLLASVMKPD 231 Query: 248 GYLVVGHSESLHGMDLGLDMQSPSI 272 + VG +E G+ LG M+S I Sbjct: 232 ALIFVGPAEG--GILLGPTMESAGI 254 >gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransferase chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 67.8 bits (164), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 51/189 (26%), Positives = 89/189 (47%), Gaps = 12/189 (6%) Query: 77 IDLITTNETYFFREPGHFDFVVGQWLPQLAQQPQLRIWSAASATGEEAYTLAMLLDHHRP 136 ++ +T + FR+P +F + + +P L P L+IW A ++GEE Y+LA+LL Sbjct: 75 MEYLTVQVSEMFRDPSYFLALREKVVPLLRTYPSLKIWVAGCSSGEEVYSLAILLREEGL 134 Query: 137 GASWSVFASDISTRVLRFARRALYPMVRGQNIPRAYLRRYCLMGVGEY----EGHFLVQA 192 ++A+DI+ L+ A +Y + R + + + +Y G + Sbjct: 135 LERSIIYATDINQSALKKAEAGVYDLERIAGFTNNHQKSGARTSLSDYYTAAYGAVVFDK 194 Query: 193 DLRAKVQFAQRNLTRLDEHDDGQYDLIFLRNVIIYFD---CETKLQVLRDVIARLRPGGY 249 LR V F+ +L + + L+ RNV+IYF+ E L + R+ + R G+ Sbjct: 195 SLRKNVVFSDHSLA--TDSVFAEMHLVSCRNVLIYFNRQLQERALGLFRESLVR---KGF 249 Query: 250 LVVGHSESL 258 L +G ESL Sbjct: 250 LGLGSKESL 258 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 839,132 Number of extensions: 31910 Number of successful extensions: 122 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 112 Number of HSP's successfully gapped: 5 Length of query: 277 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 193 Effective length of database: 1,222,130 Effective search space: 235871090 Effective search space used: 235871090 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)