BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0139.p (725 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 1440 0.0 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 325 1e-89 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 249 4e-67 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 244 1e-65 gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Her... 181 2e-46 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 1440 bits (3728), Expect = 0.0, Method: Compositional matrix adjust. Identities = 722/725 (99%), Positives = 722/725 (99%) Query: 1 MSKDSARHAMVQEARELLVAMEAALLQTEMEGPSRDSIHAIFRAAHTIKGSAGLFAFDSI 60 MSKDSARHAMVQEARELLVAMEAALLQTEMEGPSRDSIHAIFRAAHTIKGSAGLFAFDSI Sbjct: 1 MSKDSARHAMVQEARELLVAMEAALLQTEMEGPSRDSIHAIFRAAHTIKGSAGLFAFDSI 60 Query: 61 VQFTHHLEHVLERVREERLALDSHLMSLLLQCSDYIGELVDAIERGQEAEEPNAERRAAL 120 VQFTHHLEHVLERVREERLALDSHLMSLLLQCSDYIGELVDAIERGQEAEEPNAERRAAL Sbjct: 61 VQFTHHLEHVLERVREERLALDSHLMSLLLQCSDYIGELVDAIERGQEAEEPNAERRAAL 120 Query: 121 LAELDAVAAFSVDHAPASVPVPLAALRGAAGSESVTADQAATRVPYWHLSLQLNENVLRD 180 LAELDAVAAFSVDH PASVPVPLAALRGAAGSES TADQAATRVPYWHLSLQLNENVLRD Sbjct: 121 LAELDAVAAFSVDHGPASVPVPLAALRGAAGSESATADQAATRVPYWHLSLQLNENVLRD 180 Query: 181 GLDPLSFLHYLRSLGRIVALVTVESAIPEAAAMDAESCYLGMEVCLASDSTLQTLENVFE 240 GLDPLSFLHYLRSLGRIVALVTVESAIPEAAAMDAESCYLGMEVCLASDSTLQTLENVFE Sbjct: 181 GLDPLSFLHYLRSLGRIVALVTVESAIPEAAAMDAESCYLGMEVCLASDSTLQTLENVFE 240 Query: 241 FVRDDSRIDILPPRAPLSAYAERLARLAPQDAARLAAAWRGLALFDQAQWQSLEAASATP 300 FVRDDSRIDILPPRAPLSAYAERLARLAPQDAARLAAAWRGLALFDQ QWQSLEAASATP Sbjct: 241 FVRDDSRIDILPPRAPLSAYAERLARLAPQDAARLAAAWRGLALFDQTQWQSLEAASATP 300 Query: 301 SDALAAAVEPMAAEGVLQRPRALDERRAQEQKFIKIEVSKLDQLIDLVGELVIAGASARL 360 SDALAAAVEPMAAEGVLQRPRALDERRAQEQKFIKIEVSKLDQLIDLVGELVIAGASARL Sbjct: 301 SDALAAAVEPMAAEGVLQRPRALDERRAQEQKFIKIEVSKLDQLIDLVGELVIAGASARL 360 Query: 361 IARRRKDSQFEEATQAIDTLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDLGKDIDL 420 IARRRKDSQFEEATQAIDTLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDLGKDIDL Sbjct: 361 IARRRKDSQFEEATQAIDTLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDLGKDIDL 420 Query: 421 VMTGSDTELDKSMVERLADPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGS 480 VMTGSDTELDKSMVERLADPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGS Sbjct: 421 VMTGSDTELDKSMVERLADPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGS 480 Query: 481 VVIEVMDDGRGMDREAILAKAVAQGLASPDAELSDAEIYRFVFEPGFSTARQVTELSGRG 540 VVIEVMDDGRGMDREAILAKAVAQGLASPDAELSDAEIYRFVFEPGFSTARQVTELSGRG Sbjct: 481 VVIEVMDDGRGMDREAILAKAVAQGLASPDAELSDAEIYRFVFEPGFSTARQVTELSGRG 540 Query: 541 VGMDVVRRNIEALHGEVSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVV 600 VGMDVVRRNIEALHGEVSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVV Sbjct: 541 VGMDVVRRNIEALHGEVSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVV 600 Query: 601 ECIDMPPQQPGSHIVNVRDEPLPFVPLRELFDLPARERGRNSLVIVQYGPLRAGLLVDGL 660 ECIDMPPQQPGSHIVNVRDEPLPFVPLRELFDLPARERGRNSLVIVQYGPLRAGLLVDGL Sbjct: 601 ECIDMPPQQPGSHIVNVRDEPLPFVPLRELFDLPARERGRNSLVIVQYGPLRAGLLVDGL 660 Query: 661 LGECQAVIKPLGRLFGKVKGLSGSTILGDGRVALILDIAHLVHHTQQQEQGSAQAYAERG 720 LGECQAVIKPLGRLFGKVKGLSGSTILGDGRVALILDIAHLVHHTQQQEQGSAQAYAERG Sbjct: 661 LGECQAVIKPLGRLFGKVKGLSGSTILGDGRVALILDIAHLVHHTQQQEQGSAQAYAERG 720 Query: 721 VASAP 725 VASAP Sbjct: 721 VASAP 725 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 325 bits (832), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 175/390 (44%), Positives = 254/390 (65%), Gaps = 11/390 (2%) Query: 328 AQEQKFIKIEVSKLDQLIDLVGELVIAGASARLIARRRKDSQFEEATQAIDTLMEQIRD- 386 E I++ V K+DQLI+LVGELVI A + E ++ L RD Sbjct: 301 GHESSSIRVSVEKVDQLINLVGELVITQAMIEQRVGKLDPMTHEHLLNSVSQLSRNTRDL 360 Query: 387 --AALTLRMVQINEVFQRFPRIVRDIARDLGKDIDLVMTGSDTELDKSMVERLADPLMHI 444 + +++RM+ ++ VF RFPR+VRD+A LGK ++ V G+ TELDK ++ER+ DPL H+ Sbjct: 361 QESVMSIRMMPMDYVFSRFPRMVRDLAHKLGKQVEFVTHGAATELDKGLIERIVDPLTHL 420 Query: 445 VRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGRGMDREAILAKAVAQ 504 VRN+IDHGIE R A+GK +L L+A H+ G+++IEV DDG G++RE ILAKA Q Sbjct: 421 VRNSIDHGIELPDARRASGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAKAKQQ 480 Query: 505 GLASPDAELSDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNIEALHGEVSIDTGRG 564 G+ D LSDAE+++ +FEPGFSTA QVT++SGRGVGMDVV+RNI A+ G V I + +G Sbjct: 481 GMPVTD-NLSDAEVWQLIFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVDIRSSKG 539 Query: 565 RGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECI-----DMPPQQPGSHIVNVRD 619 GT + + LPLTLAI+ G + +G V+++PL VVE + D+ ++ VR Sbjct: 540 FGTTISISLPLTLAILDGMSIRIGEEVYILPLGYVVESLQPLAQDIKEISGQGQVLRVRG 599 Query: 620 EPLPFVPLRELFDL-PA-RERGRNSLVIVQYGPLRAGLLVDGLLGECQAVIKPLGRLFGK 677 E LP +PL ++F++ P+ + + +VI++ +A L VD L+G+ Q V+K L + K Sbjct: 600 EYLPLIPLYQIFNIEPSFTDPSQGLVVILESDGKKAALFVDDLIGQQQIVVKNLESNYRK 659 Query: 678 VKGLSGSTILGDGRVALILDIAHLVHHTQQ 707 V G+SG+TILGDG VALI+D+A L+ ++Q Sbjct: 660 VAGISGATILGDGGVALIVDVAALLRSSRQ 689 Score = 63.9 bits (154), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 30/91 (32%), Positives = 52/91 (57%) Query: 21 MEAALLQTEMEGPSRDSIHAIFRAAHTIKGSAGLFAFDSIVQFTHHLEHVLERVREERLA 80 ME LL + P + ++A+FRAAH+IKG + F I + TH LE +L+R+R+ ++A Sbjct: 1 MEKLLLAVNVASPDPEDLNAVFRAAHSIKGGSSTFGLTDIAEVTHVLESLLDRIRQGQMA 60 Query: 81 LDSHLMSLLLQCSDYIGELVDAIERGQEAEE 111 L S + L D + ++D + G + ++ Sbjct: 61 LTSEHVDAFLAAKDVLKSMLDGHKHGADVDQ 91 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 249 bits (637), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 145/397 (36%), Positives = 243/397 (61%), Gaps = 28/397 (7%) Query: 326 RRAQE----QKFIKIEVSKLDQLIDLVGELVIAGASARLIARRRKDS--------QFEEA 373 RRA+E K +K+E K+D+L++L+GE+V++ + +A+R +D + + Sbjct: 439 RRAEESLSGNKSVKVEQVKIDRLMNLIGEMVVSKNALPYLAQRAEDQFGVRELAREIKAQ 498 Query: 374 TQAIDTLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDLGKDIDLVMTGSDTELDKSM 433 I+ + E+++DA + +RM+ ++ VFQRFPR+VRDI+R LGK++ LV+ G +TE DK++ Sbjct: 499 YAVINRIAEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNI 558 Query: 434 VERLADPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGRGMD 493 +E LADPL+HIVRN++DHG+E +R A+GKPA SL ++A HE VVI + DDG+G+D Sbjct: 559 IEALADPLIHIVRNSLDHGLEGPEQRRASGKPALGSLTISAAHEGDQVVIVIEDDGKGID 618 Query: 494 REAILAKAVAQGLASPDAEL---SDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNI 550 I KA +GL +A+L SD + VF GFSTA V++LSGRGVGMDVVR + Sbjct: 619 PALIKRKAYEKGLID-EAQLERISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAV 677 Query: 551 EALHGEVSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPQQP 610 E ++G +++ + G+GT +++ LPL++A+ + +F +P++ V+E + +P + Sbjct: 678 EKVNGSLALHSVSGKGTRLQISLPLSMAVTKVMIIESDGQLFGVPMEHVMETVRVP--RS 735 Query: 611 GSHIVN------VRDEPLPFVPLRELFDLPARER----GRNSLVIVQYGPLRAGLLVDGL 660 H N +R L + ++ + A ++ G ++++V+ G GL++D Sbjct: 736 SLHFANSSAKMVLRGHILGLKGINQILGIAAEQKTNADGEVAVLVVRVGGQSVGLVIDDF 795 Query: 661 LGECQAVIKPLGRLFGKVKGLSGSTILGDGRVALILD 697 + KPL + G + SGS ++GDG V ++L+ Sbjct: 796 RETVDVIQKPLLGILGGLSLYSGSALMGDGSVLMVLN 832 Score = 58.5 bits (140), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 32/109 (29%), Positives = 56/109 (51%) Query: 7 RHAMVQEARELLVAMEAALLQTEMEGPSRDSIHAIFRAAHTIKGSAGLFAFDSIVQFTHH 66 R + E R+LL + L+Q E + + ++FR HT+KG++GLF + + H Sbjct: 5 RDQFILETRDLLQGISEKLMQLEDAPEHVELLESLFRMVHTLKGNSGLFDVPEMTRVLHA 64 Query: 67 LEHVLERVREERLALDSHLMSLLLQCSDYIGELVDAIERGQEAEEPNAE 115 E +++ VR ++A L LL D++ L D +E E +P+A+ Sbjct: 65 CEDLMDAVRRRQVAYSEVLADRLLDAMDFVAMLCDGLEMEAEQADPHAQ 113 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 244 bits (624), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 151/415 (36%), Positives = 241/415 (58%), Gaps = 19/415 (4%) Query: 309 EPMAAEGVLQRPRALDERRAQEQKFIKIEVSKLDQLIDLVGELVIAGASARLIARRRKDS 368 EP AA RA D +++ I+I+ KLD L+++ GE V A A ++ + + Sbjct: 226 EPKAAAPAQHNRRASDSPGGAKEESIRIDAGKLDALLEVAGESVQAANQAAVLLEKLQQF 285 Query: 369 QFEEATQAI-DTLME----------QIRDAALTLRMVQINEVFQRFPRIVRDIARDLGKD 417 +FE A+ TL E +++ A L+ RM + +FQ+FPR+VR++A+DLGKD Sbjct: 286 KFEGTAAALMSTLAETLGRASRYSSELQRATLSTRMQPVGRLFQKFPRLVRELAKDLGKD 345 Query: 418 IDLVMTGSDTELDKSMVERLADPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHE 477 +DLV+TG++TE+D+ +V+ L DPL+H++RN++DHGIE +R+AAGKP KA++ LNA Sbjct: 346 VDLVITGAETEVDRVVVDSLYDPLVHMLRNSLDHGIE--VDRVAAGKPTKATISLNAWQA 403 Query: 478 SGSVVIEVMDDGRGMDREAILAKAVAQGLASPDAELSDAEIYRFVFEPGFSTARQVTELS 537 SV+IEV DDG+GMD + + +KA+A+GL + L+ E + VF PGFST + +S Sbjct: 404 GSSVMIEVFDDGKGMDPDMLRSKALAKGLINEHQALTADEALQLVFLPGFSTKEVASSVS 463 Query: 538 GRGVGMDVVRRNIEALHGEVSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLD 597 GRGVGMDVV+ +E G + I++ G+GT +RLP+ L+II V A +P+ Sbjct: 464 GRGVGMDVVKTAVEKHRGTIRIESTIGKGTKFSIRLPIELSIIPTMLVRCAGAPLALPMA 523 Query: 598 MVVECIDMPPQ--QPGSHIVNVRDEPLPFVPLRELFDLPARERGRNSLVIVQYGPLRAGL 655 +V +++P + G V +RD+ P P+ L + E G + IV P L Sbjct: 524 VVERVVELPENFDEVGGAPV-LRDQGRPL-PVNSLAGVLGYEPGNERVGIVMAVPHPYIL 581 Query: 656 LVDGLLGECQAVIKPLGRLFGKVKGLSGSTILGDGRVALILDIAHLVHHTQQQEQ 710 V+ + G VIKPL + G++G+ +G + L++ ++ L+ + E+ Sbjct: 582 GVESVEGTADLVIKPLTAI--HTAGITGTARSAEGELVLVVGLSFLLDGCRDMER 634 >gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 181 bits (459), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 113/314 (35%), Positives = 173/314 (55%), Gaps = 9/314 (2%) Query: 392 RMVQINEVFQRFPRIVRDIARDLGKDIDLVMTGSDTELDKSMVERLADPLMHIVRNAIDH 451 RMV+ N + R +VR +A + GK + L ++ ++D++++ERL PL H++RNA H Sbjct: 412 RMVKFNSMEVRMQHLVRQVAAETGKPLALDLSNCRLQIDRAILERLMGPLEHLLRNAAVH 471 Query: 452 GIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGRGMDREAILAKAVAQGLASPDA 511 GIE A +RLA GKPA L + A HE VI V DDGRG+D EAI KA+ G A+ A Sbjct: 472 GIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRVSDDGRGLDLEAIRQKALELG-AAGLA 530 Query: 512 ELSDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNIEALHGEVSIDTGRGRGTVVRL 571 E S A + +F+PG ST+ QV+ L+GRG+GMDVVR + AL G + + + G+G L Sbjct: 531 EASQARLADLIFQPGLSTSPQVSALAGRGIGMDVVRSEVAALGGWMRVHSQPGQGVQFTL 590 Query: 572 RLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPQQPGSHI----VNVRDEPLPFVPL 627 LPL+LA+ + G F IP +V + + Q + + + L P Sbjct: 591 YLPLSLAVQHVCLLRHGQQHFAIPSMLVDSIVQLRGPQARQALAERSLEHQGSRLALHPW 650 Query: 628 RELFDLPARERGRNS----LVIVQYGPLRAGLLVDGLLGECQAVIKPLGRLFGKVKGLSG 683 L D G S +++++ +L D + G + V+KP+G + G+ G Sbjct: 651 HCLLDEAPEPAGEGSDSAYVLLIKRDDGLMAVLADHVSGNREVVVKPVGPQLATLAGVVG 710 Query: 684 STILGDGRVALILD 697 +T+LGDG++ LI++ Sbjct: 711 ATVLGDGQIVLIIN 724 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,803,514 Number of extensions: 69719 Number of successful extensions: 377 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 364 Number of HSP's successfully gapped: 10 Length of query: 725 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 633 Effective length of database: 1,184,250 Effective search space: 749630250 Effective search space used: 749630250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)