BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0101.p (428 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 860 0.0 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 199 5e-52 gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 166 5e-42 gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 124 2e-29 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 123 2e-29 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 860 bits (2221), Expect = 0.0, Method: Compositional matrix adjust. Identities = 428/428 (100%), Positives = 428/428 (100%) Query: 1 MEVQKEKSPRLKRIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSA 60 MEVQKEKSPRLKRIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSA Sbjct: 1 MEVQKEKSPRLKRIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSA 60 Query: 61 FSLAYAFSQLPIGAMLDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEA 120 FSLAYAFSQLPIGAMLDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEA Sbjct: 61 FSLAYAFSQLPIGAMLDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEA 120 Query: 121 PQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVL 180 PQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVL Sbjct: 121 PQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVL 180 Query: 181 GIAVSVGWYLFYRNRNEVQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGML 240 GIAVSVGWYLFYRNRNEVQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGML Sbjct: 181 GIAVSVGWYLFYRNRNEVQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGML 240 Query: 241 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR 300 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR Sbjct: 241 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR 300 Query: 301 GMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVA 360 GMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVA Sbjct: 301 GMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVA 360 Query: 361 IPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMFVV 420 IPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMFVV Sbjct: 361 IPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMFVV 420 Query: 421 DKPIESHA 428 DKPIESHA Sbjct: 421 DKPIESHA 428 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 199 bits (505), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 128/424 (30%), Positives = 216/424 (50%), Gaps = 17/424 (4%) Query: 13 RIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQLPI 72 ++ + L L + VNY DR+TLSIA ++ EL+L MG + SAFS AY +QLP Sbjct: 10 HVRYLILFLLFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQLPG 69 Query: 73 GAMLDKFGARVMLGAGMFIWSVAQLAGGFVN------NLQQFLVARVFLGLGEAPQFPAG 126 G +LD+FG++ + + +WSV V + + R +GL EAP FPA Sbjct: 70 GWLLDRFGSKKIYMWSIALWSVFTFLQAGVTWMSTAAAVASLFLLRFMVGLAEAPSFPAN 129 Query: 127 AKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVSV 186 ++V+ WF +ERG + IF ++ L P++ ++ ++ W +F +MGV+GIA+++ Sbjct: 130 GRIVAAWFPTKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGIALAL 189 Query: 187 GW-YLFYRNRNEVQLTPSEVLYLAD-----DHDNDPNANAKLSWSEW-KSLFTYRTTWGM 239 W + Y ++ ++ +E+ ++ D DN A + + L + R G+ Sbjct: 190 IWGRIIYSPKDHPRINQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRMLLGV 249 Query: 240 LFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMK 299 G + + + +LTW P YL ER +SI + G+V IP + G +G + G I+D L+K Sbjct: 250 YIGQYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISDRLIK 309 Query: 300 RGMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGG-AWALVS 358 RG+ +RK PI G++ I Y + M + +A+AF G WA+V+ Sbjct: 310 RGLSVTWARKIPIVGGML-LSMTMILCNYVEAQWMVVGI--MALAFFGKGVGALGWAVVA 366 Query: 359 VAIPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMF 418 P+++V G + N G + G P++ G +V +T SF AL+ A + Y+ Sbjct: 367 DTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKETGSFSGALVFVGINALVTVISYLV 426 Query: 419 VVDK 422 +V + Sbjct: 427 IVKE 430 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 166 bits (419), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 112/388 (28%), Positives = 188/388 (48%), Gaps = 10/388 (2%) Query: 11 LKRIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQL 70 ++ I+ ++ + LA IVNYL R+TLS+A ++ +EL++S Q ++ A+ + YA Q Sbjct: 4 VRGIRWYMVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQP 63 Query: 71 PIGAMLDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQFPAGAKVV 130 G +LD G ++ G WS+ A F Q R LG+ EA P G K Sbjct: 64 VAGYILDAVGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGVKAS 123 Query: 131 SEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVSVGWYL 190 +EWF +ER G F S+IG APP++ +L GW+M FV++G LG+ V W L Sbjct: 124 TEWFPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLWML 183 Query: 191 FYRN-RNEVQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGMLFGFMGVIYM 249 FY++ R++ L+P E Y+ + + P + SW++ + R W + Sbjct: 184 FYKSPRDQKLLSPEERAYILEGQEKSPEKVQRESWTK---IVRSRNFWSIAIPRFLSEPA 240 Query: 250 VWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR-GMPAIRSR 308 + W+P Y+ ERH++I +P++ +G + GY++ K + SR Sbjct: 241 WQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSR 300 Query: 309 KWPICAGLIGAGAFTIPAA--YTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIPRRLV 366 K L+G+ + PA + S +AI +S+ SG +++ S + V Sbjct: 301 K---LVMLVGSLSMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQV 357 Query: 367 ASLGSMQNFGGYLGGSLAPVITGVVVDQ 394 A+ + GYLG +L ++ G++V Q Sbjct: 358 ATATGLTGMSGYLGATLFTLLFGILVTQ 385 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 124 bits (310), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 86/308 (27%), Positives = 143/308 (46%), Gaps = 7/308 (2%) Query: 22 LMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQLPIGAMLDKFGA 81 + L I NYL R++L + ++ EL +S Q +++AF + Y Q G ++D G Sbjct: 15 VCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTIMQPVCGFIVDLIGL 74 Query: 82 RVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQFPAGAKVVSEWFAMRERGG 141 R+ +WSVA + R +GL EA P+G K V+EWF +E+ Sbjct: 75 RIGFALFGVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGMKAVAEWFPDKEKSV 134 Query: 142 PTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVSVGWYLFYRN-RNEVQL 200 G F + +++G LAPP++ L + +GW+ FV+ G+LG + W+ FYR+ R+ L Sbjct: 135 AVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAALWFAFYRSPRDHAAL 194 Query: 201 TPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGMLFGFMGVIYMVWLYLTWLPAY 260 + SE + +A K + R W + + W+P Y Sbjct: 195 SDSERETIISGQLRP--VSAVRGKRPVKDVVRSRRFWAIALARFFAEPAWQTFSFWIPLY 252 Query: 261 LEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR-GMPAIRSRKWPICAGLIGA 319 +ERHL + +P++ LG L+ GY++ L+ R +P + SR +C ++GA Sbjct: 253 FVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRFRIPLVWSR---VCGVVLGA 309 Query: 320 GAFTIPAA 327 PA Sbjct: 310 VMMIGPAC 317 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 123 bits (309), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 97/326 (29%), Positives = 152/326 (46%), Gaps = 26/326 (7%) Query: 3 VQKEKSPRLKRIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFS 62 +Q K R V AL F++ V DR+ L +A + +E +S ++ G L S F Sbjct: 6 IQHTKKKTHFRWVVGALIFIVYT--VAAADRANLGVALPFLRKEFVMSNTEAGALASIFL 63 Query: 63 LAYAFSQLPIGAMLDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQ 122 AY+ +QLP G +FG + M SV G ++L + R LGL E P Sbjct: 64 FAYSLAQLPSGLAYTRFGIPRVFSLAMLATSVFTGLIGTASSLLALKIFRFGLGLAEGP- 122 Query: 123 FPAG-AKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLG 181 P G ++ WF RE+G TGIF+A++ GP L PP+ ++M +GWR +F V G Sbjct: 123 LPIGITSTINNWFPAREKGTMTGIFLAAAKFGPVLVPPVCAVIMYYWGWRHIFYFFAVPG 182 Query: 182 IAVSVGWYLFYRNR--NEVQLTPSEVLYLADD------HDNDPNANAKLSW-------SE 226 + +SV W + +NR ++ E+ ++ D D DP + S+ Sbjct: 183 VILSVVWLMVVKNRPSESPHVSAEELEHINDSQGAAAGQDADPAVTRDFALLDRIIRRSK 242 Query: 227 WKSLFTYR---TTWGMLFGFMGVIYM---VWLYLTWLPAYLEHERHLSIARTGWVVVIPY 280 ++ T + +W ++ +G +M V + L W+P YL + SIA G V P+ Sbjct: 243 VATIETTKGVFCSWNIMGAALGYFFMTAIVNVLLAWIPTYLISVKGYSIANMGMVAAAPW 302 Query: 281 IFGTLGMLSSGYIADG-LMKRGMPAI 305 + +G L G + D L KR PA+ Sbjct: 303 VGAVIGNLLGGAMTDRWLEKRRKPAM 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,254,074 Number of extensions: 51865 Number of successful extensions: 360 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 323 Number of HSP's successfully gapped: 29 Length of query: 428 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 340 Effective length of database: 1,203,190 Effective search space: 409084600 Effective search space used: 409084600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)