BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0100.p (241 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 474 e-135 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 135 2e-33 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 126 1e-30 gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 124 1e-29 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 119 2e-28 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 474 bits (1219), Expect = e-135, Method: Compositional matrix adjust. Identities = 239/241 (99%), Positives = 239/241 (99%) Query: 1 MSFIPDLLKGKTALVTGGTQGIGAAVARQLAALGARVIAAGLPPFAHEEFAGSAVTLVEM 60 MSFIPDLLKGKTALVTGGTQGIGAAVARQL ALGARVIAAGLPPFAHEEFAGS VTLVEM Sbjct: 1 MSFIPDLLKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPPFAHEEFAGSEVTLVEM 60 Query: 61 NVASEESVRAALAGLTQLDIVVNCAGIIKRGAELETEVFEQVIAVNLTGTMRVCAAAREL 120 NVASEESVRAALAGLTQLDIVVNCAGIIKRGAELETEVFEQVIAVNLTGTMRVCAAAREL Sbjct: 61 NVASEESVRAALAGLTQLDIVVNCAGIIKRGAELETEVFEQVIAVNLTGTMRVCAAAREL 120 Query: 121 LRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIA 180 LRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIA Sbjct: 121 LRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIA 180 Query: 181 TPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGGYLV 240 TPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGGYLV Sbjct: 181 TPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGGYLV 240 Query: 241 A 241 A Sbjct: 241 A 241 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 135 bits (341), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 89/252 (35%), Positives = 126/252 (50%), Gaps = 26/252 (10%) Query: 8 LKGKTALVTGGTQGIGAAVARQLAALGARVIAA------------GLPPFAHEEFAGSAV 55 +GK +VTG GIG A AR+ + GARV+ A LPP E A Sbjct: 12 FEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPP---ERTAAR-- 66 Query: 56 TLVEMNVASEESVRA----ALAGLTQLDIVVNCAGIIKRG--AELETEVFEQVIAVNLTG 109 E +V+ E VR A+ QLD++V+ AG+ G E+ E + +V A N+ G Sbjct: 67 ---ETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNG 123 Query: 110 TMRVCAAARELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGI 169 A L ++RGCIVN AS+ + Y+ASKG V LT+++A+ + G+ Sbjct: 124 VFYGAREALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGV 183 Query: 170 RVNAVAPGWIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTG 229 R+NAV P T +T + DDP R LGR P ++ V+ FL SP ASF+ G Sbjct: 184 RINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASFVNG 243 Query: 230 AIVPVDGGYLVA 241 +PVDGG + + Sbjct: 244 VNLPVDGGLMAS 255 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 126 bits (317), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 84/242 (34%), Positives = 136/242 (56%), Gaps = 14/242 (5%) Query: 11 KTALVTGGTQGIGAAVARQLAALGARVIAAGLPPFAHEEFA------GSAVTLVEMNVAS 64 + ALVTGG GIGA++++QLAA G V+ A + A + A G ++M++ S Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 65 EESVRAALAGLTQ----LDIVVNCAGIIKRGAELET--EVFEQVIAVNLTGTMRV-CAAA 117 ES+ AA A + + D++VN AG+ + + L+ E + Q + VN+TG + AA Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAA 128 Query: 118 RELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPG 177 R +++Q G IVN +S+ G Y SK V LT+ +AI A GI VN+VAPG Sbjct: 129 RLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPG 188 Query: 178 WIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGG 237 + TP+TQA+ + R P+ R+G +++ V +L + ++++TG ++PVDGG Sbjct: 189 PVDTPMTQAMHSEQTRE-SYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGG 247 Query: 238 YL 239 ++ Sbjct: 248 FV 249 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 124 bits (310), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 97/248 (39%), Positives = 133/248 (53%), Gaps = 18/248 (7%) Query: 1 MSFIPDLLKGKTALVTGGTQGIGAAVARQLAALGARVIAAGLPPFAHE-----EFAGSAV 55 MSF GKT ++TG +GIG A A LA GA+VIA L A + E G+ V Sbjct: 1 MSF-----AGKTVIITGAGRGIGRACALLLAQRGAQVIA--LARTASDLARLQEEIGARV 53 Query: 56 TLVEMNVASEESVRAALAGLTQLDIVVNCAGI--IKRGAELETEVFEQVIAVNLTGTMRV 113 V+++ + A AG D ++N AGI ++ E+ E +E V+ VNL + Sbjct: 54 IAVDLSDPAAARRAMAEAGTA--DFLINSAGINVLESVLEMSDEGYEAVLGVNLRAALIT 111 Query: 114 CAA-ARELLRQSRG-CIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRV 171 C A AR + Q G +VN S+ G Y+ASK G+ T+ LA GIRV Sbjct: 112 CQAFARMRIAQGGGGAMVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRV 171 Query: 172 NAVAPGWIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAI 231 NAVAP T L +A DP ++GP++AR P R+ PEDV + +A L SP A+ + GA+ Sbjct: 172 NAVAPTITLTELAEAAWSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAV 231 Query: 232 VPVDGGYL 239 VPVDGG+L Sbjct: 232 VPVDGGFL 239 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 119 bits (299), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 82/243 (33%), Positives = 122/243 (50%), Gaps = 13/243 (5%) Query: 8 LKGKTALVTGGTQGIGAAVARQLAALGARVIAAGLPP------FAHEEFAGSAVTLVEMN 61 L+GK AL+TG + GIG A+AR +A GA ++ G A G + + Sbjct: 17 LEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIHESSFD 76 Query: 62 VASEESVRAALAGLTQ----LDIVVNCAGIIKRG--AELETEVFEQVIAVNLTGTMRVC- 114 S E V+AA+ + + I+VN AGI +R E + + +I+ NL Sbjct: 77 ATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFAAQ 136 Query: 115 AAARELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAV 174 A AR ++ + G I+N S+ S G + Y+A+KG V TK +AI + GI+VN + Sbjct: 137 AVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGL 196 Query: 175 APGWIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPV 234 PG+ T + AL +D ++ RTP RWG ED+ FL S A+ F+ G I+ V Sbjct: 197 GPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILYV 256 Query: 235 DGG 237 DGG Sbjct: 257 DGG 259 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 732,160 Number of extensions: 29505 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 48 Number of HSP's gapped: 182 Number of HSP's successfully gapped: 48 Length of query: 241 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 158 Effective length of database: 1,226,865 Effective search space: 193844670 Effective search space used: 193844670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)