BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0090.p (404 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309906|ref|YP_003773998.1| major facilitator superfamily p... 802 0.0 gi|300313658|ref|YP_003777750.1| transport transmembrane protein... 276 2e-75 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 124 1e-29 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 112 6e-26 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 88 2e-18 >gi|300309906|ref|YP_003773998.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 802 bits (2071), Expect = 0.0, Method: Compositional matrix adjust. Identities = 401/404 (99%), Positives = 402/404 (99%) Query: 1 MICLSALFLAWAVGYADRIVMSTAIIPISKEFALDAQQAGMVLSAFYVSYALMQLMGGWL 60 MICLSALFLAWAVGYADRIVMSTAIIPISKEFALDAQQAGMVLSAFYVSYALMQLMGGWL Sbjct: 11 MICLSALFLAWAVGYADRIVMSTAIIPISKEFALDAQQAGMVLSAFYVSYALMQLMGGWL 70 Query: 61 SDRYGSRIVVVMCVVAWSLFTGLTSMAWSFASLLLIRFLFGAGEGCFSPASSVTVAEVFP 120 SDRYGSRIVVVMCVVAWSLFTGLTSMAWSFASLLLIRFLFGAGEGCFSPASSVTVAEVFP Sbjct: 71 SDRYGSRIVVVMCVVAWSLFTGLTSMAWSFASLLLIRFLFGAGEGCFSPASSVTVAEVFP 130 Query: 121 QQERARAKSFLISTVFLGNAVGSGMVALTVVYLGWRGTFHILAFVGMIVAAILWLALRSG 180 QQERARAKSFLISTVFLGNAVGSGMVALTVVYLGWRGTFHILAFVGMIVAAILWLALRSG Sbjct: 131 QQERARAKSFLISTVFLGNAVGSGMVALTVVYLGWRGTFHILAFVGMIVAAILWLALRSG 190 Query: 181 MAAQQARSAARPKNQLRELVKNPTALRLTAIWFCTSIVYIGMISWMPSFLLKVYQIDLLH 240 MAAQQARSAARPKNQLRELVKNPTALRLTAIWFCTSIVYIGMISWMPSFLLKVYQIDLLH Sbjct: 191 MAAQQARSAARPKNQLRELVKNPTALRLTAIWFCTSIVYIGMISWMPSFLLKVYQIDLLH 250 Query: 241 IGIASAIPYLLAFLGTNVVGWLLDKHGKGREKYLMMGGALACAVFLALMISTTVIELLVV 300 IGIASAIPYLLAFLGTNVVGWLLDKHGKGREKY MMGGALACAVFLALMISTTVIELLVV Sbjct: 251 IGIASAIPYLLAFLGTNVVGWLLDKHGKGREKYFMMGGALACAVFLALMISTTVIELLVV 310 Query: 301 YWTLCLLSFNFVYATVFSVPLKHFPSHLIGSATGLMNFGGQLAGSIAPVVMGSLIVAFNG 360 YWTLCLLSFNFVYATVFSVPLKHFPSHLIGSATGLMNFGGQLAGSIAPVVMGSLIVAFNG Sbjct: 311 YWTLCLLSFNFVYATVFSVPLKHFPSHLIGSATGLMNFGGQLAGSIAPVVMGSLIVAFNG 370 Query: 361 AYLAAFWFLVGAAMMSFLIAMTWRLAPPAAPATTLPETSSSHTS 404 AYLAAFWFLVG+AMMSFLIAMTWRLAPPAAPA TLPETSSSHTS Sbjct: 371 AYLAAFWFLVGSAMMSFLIAMTWRLAPPAAPAATLPETSSSHTS 414 >gi|300313658|ref|YP_003777750.1| transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 276 bits (706), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 148/375 (39%), Positives = 231/375 (61%), Gaps = 2/375 (0%) Query: 10 AWAVGYADRIVMSTAIIPISKEFALDAQQAGMVLSAFYVSYALMQLMGGWLSDRYGSRIV 69 W V Y DR ++ A I+ EF L G++LS+F++ Y+L+QL GGWL+DRYGSR V Sbjct: 21 GWCVSYIDRAAITFAATQIASEFHLKPSDLGILLSSFFLGYSLLQLPGGWLADRYGSRPV 80 Query: 70 VVMCVVAWSLFTGLTSMAWSFASLLLIRFLFGAGEGCFSPASSVTVAEVFPQQERARAKS 129 +V+ ++ WSLFTG+T MAWS SL++IRF+FG GEG F AS VAE F + ER + S Sbjct: 81 IVISILLWSLFTGVTGMAWSVTSLVMIRFIFGVGEGAFPAASVKGVAENFSRDERPKMSS 140 Query: 130 FLISTVFLGNAVGSGMVALTVVYLGWRGTFHILAFVGMIVAAILWLALRSGMAAQQARSA 189 L+S+ ++G+ + ++A +++ GWR FH + G++ A + W +R + + Sbjct: 141 LLMSSNYIGSMLAPLLIAPLILHYGWRAVFHYIGIAGVVFALVYWFCVRP-VRPGVGGAG 199 Query: 190 ARPKNQLRELVKNPTALRLTAIWFCTSIVYIGMISWMPSFLLKVYQIDLLHIGIASAIPY 249 A K L+K P ++ A+WF SI+ G+ +WMP++L+ V ++L +G+ +PY Sbjct: 200 AINKQAFMALMKMPLMWQIVAVWFGLSIINKGLDTWMPTYLMTVRGLNLKAVGLLLPLPY 259 Query: 250 LLAFLGTNVVGWLLDKHGKGREKYLMMGGALACAVFLALMISTTVIELLVVYWTLCLLSF 309 ++A L T + GW++ + GREK L++G +L AVF+ M S +E ++ + L Sbjct: 260 IMAGLSTAIGGWVMVRFFDGREKLLLIGSSLLTAVFVYCMYSAASVEGVIAWQCLAYFFK 319 Query: 310 NFVYATVFSVPLKHFPSHLIGSATGLMNFGGQLAGSIAPVVMGSLIVAFNGAYLAAFWFL 369 +FV AT ++P K + L+GS G++N GGQLAG I+P+V+G L+ AF Y AF FL Sbjct: 320 SFVLATCIALPTKMLQAQLVGSGVGMVNLGGQLAGFISPLVIGFLVSAFAN-YDYAFSFL 378 Query: 370 VGAAMMSFLIAMTWR 384 +GAA S L+++ R Sbjct: 379 IGAAFFSVLVSLFIR 393 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 111/400 (27%), Positives = 186/400 (46%), Gaps = 42/400 (10%) Query: 7 LFLAWAVGYADRIVMSTAIIPISKEFALDAQQAGMVLSAFYVSYALMQLMGGWLSDRYGS 66 LF+ V YADR +S A + E +LD G + SAF +Y L QL GGWL DR+GS Sbjct: 19 LFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQLPGGWLLDRFGS 78 Query: 67 RIVVVMCVVAWSLFT----GLT--SMAWSFASLLLIRFLFGAGEGCFSPASSVTVAEVFP 120 + + + + WS+FT G+T S A + ASL L+RF+ G E PA+ VA FP Sbjct: 79 KKIYMWSIALWSVFTFLQAGVTWMSTAAAVASLFLLRFMVGLAEAPSFPANGRIVAAWFP 138 Query: 121 QQERARAKSFLISTVFLGNAVGSGMVALTVVYLGWRGTFHILAFVGMIVAAILW------ 174 +ER A + S + + + ++A V W F ++ VG I A++W Sbjct: 139 TKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVG-IALALIWGRIIYS 197 Query: 175 --------------LALRSGMAAQQARSAARPKNQ----LRELVKNPTALRLTAIWFCTS 216 + G+ R A P + +R+L+ N L + +C + Sbjct: 198 PKDHPRINQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRMLLGVYIGQYCIN 257 Query: 217 IVYIGMISWMPSFLLKVYQIDLLHIGIASAIPYLLAFLGTNVVGWLLDKHGKGREKYLMM 276 ++ ++W P +L++ + +L G ++IP + F+G V G + D+ K + L + Sbjct: 258 VITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISDRLIK---RGLSV 314 Query: 277 GGALACAVFLALMISTTVI--ELLVVYWTLC-LLSFNF----VYATVFSVPLKHFPSHLI 329 A + +++S T+I + W + +++ F V A ++V P ++ Sbjct: 315 TWARKIPIVGGMLLSMTMILCNYVEAQWMVVGIMALAFFGKGVGALGWAVVADTAPKQIV 374 Query: 330 GSATGLMNFGGQLAGSIAPVVMGSLIVAFNGAYLAAFWFL 369 G + GL N G +AG P+V+G IV G++ A F+ Sbjct: 375 GLSGGLFNMFGNVAGITTPIVIG-YIVKETGSFSGALVFV 413 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 112 bits (279), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 97/377 (25%), Positives = 168/377 (44%), Gaps = 22/377 (5%) Query: 1 MICLSALFLAWAVGYADRIVMSTAIIPISKEFALDAQQAGMVLSAFYVSYALMQLMGGWL 60 ++ LS L LA V Y DR +S A +S+E L A Q G++LSAF ++YA QL G + Sbjct: 16 VVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQLPIGAM 75 Query: 61 SDRYGSRIVVVMCVVAWSLFTGLTSMAWSFASLLLIRFLFGAGEGCFSPASSVTVAEVFP 120 D++G+R+++ + WS+ + L+ R G GE PA + V+E F Sbjct: 76 LDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQFPAGAKVVSEWFA 135 Query: 121 QQERARAKSFLISTVFLGNAVGSGMVALTVVYLGWRGTFHILAFVGMIVAAILWLALRSG 180 +ER +++ +G A+ ++ + ++ GWR F I+ +G+ V+ +L R+ Sbjct: 136 MRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVSVGWYLFYRNR 195 Query: 181 MAAQQARSAARPKNQLRELVKNPTALRLTAIW-----FCTS----IVYIGMI-------S 224 Q S + N A + W + T+ ++G+I + Sbjct: 196 NEVQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGMLFGFMGVIYMVWLYLT 255 Query: 225 WMPSFLLKVYQIDLLHIGIASAIPYLLAFLGTNVVGWLLD---KHGK---GREKYLMMGG 278 W+P++L + + G IPY+ LG G++ D K G K+ + G Sbjct: 256 WLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKRGMPAIRSRKWPICAG 315 Query: 279 ALACAVFLALMISTTVIELLVVYWTLCLLSFNFVYATVFSVPLKHFPSHLIGSATGLMNF 338 + F T + +VY +L + N +++ P L+ S + NF Sbjct: 316 LIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIPRRLVASLGSMQNF 375 Query: 339 GGQLAGSIAPVVMGSLI 355 GG L GS+APV+ G ++ Sbjct: 376 GGYLGGSLAPVITGVVV 392 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 87.8 bits (216), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 92/419 (21%), Positives = 181/419 (43%), Gaps = 34/419 (8%) Query: 7 LFLAWAVGYADRIVMSTAIIPISKEFALDAQQAGMVLSAFYVSYALMQLMGGWLSDRYGS 66 + L + V Y DRI + A + ++K+ L Q G+ F++ Y ++ R+G+ Sbjct: 35 ILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYFFFEIPSNLALHRFGA 94 Query: 67 RIVVVMCVVAWSLFTGLTSMAWSFASLLLIRFLFGAGEGCFSPASSVTVAEVFPQQERAR 126 R+ + +++W L + T+ S L RFL G E F+P + + FP R + Sbjct: 95 RMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGIYLYFTQWFPGAWRGK 154 Query: 127 AKSFLISTVFLGNAVGSGMVALTVV------YLGWRGTFHILAFVGMIV----------- 169 A +F + + + N +GS + + + GW+ + A +++ Sbjct: 155 ATAFFLIGIPVANIIGSPLSGALMELHGMWGFKGWQVLLLLEALPAVLLGVMCLFLLPDR 214 Query: 170 -AAILWLA------LRSGMAAQQARSAARPKNQLRELVKNPTALRLTAIWFCTSIVYIGM 222 A WL+ L + ++ +Q AAR N+L++ N L FC I + + Sbjct: 215 PAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVFALAGANFCGIIGSLSI 274 Query: 223 ISWMPSFLLKVYQIDLLHIGIASAIPYLLAFLGTNVVGWLLDKHGKGREKYLMMGGALAC 282 W+P +++ + + +G+ +AIPYL+ + + W + R + + G + Sbjct: 275 GLWLPQ-IIREFGLPSHQVGLVAAIPYLVGAVAMTL--WARLANRSERRLFFVAGAIVLA 331 Query: 283 AVFLAL--MISTTVIELLVVYWTLCLLSFNFVYATVFSVPLKHFPSHLIGSATGLMNFGG 340 A+ L + + T ++++L + T+ + S AT +++P L+ G Sbjct: 332 ALSLGISAFLHTPLLKMLAI--TVAVASILSFQATFWAIPSGFLTGRAAAGGLALIVSIG 389 Query: 341 QLAGSIAPVVMGSLIVAFNGAYLAAFWFLVGAAMMSFLIAMTWRLAPPAAPATTLPETS 399 L G + P V+G + A G + F+ GA ++ +I + L P+ T ET+ Sbjct: 390 NLGGFVGPSVIGFIREATQG-FTYPLIFVAGALLLGAVITLA--LGDPSRVPPTSKETA 445 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.138 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,217,229 Number of extensions: 50041 Number of successful extensions: 409 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 353 Number of HSP's successfully gapped: 46 Length of query: 404 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 317 Effective length of database: 1,207,925 Effective search space: 382912225 Effective search space used: 382912225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)