BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0089.p (500 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309905|ref|YP_003773997.1| N-acyl-D-glutamate deacylase [H... 1018 0.0 gi|300311079|ref|YP_003775171.1| N-acyl-D-glutamate deacylase [H... 449 e-127 gi|300309903|ref|YP_003773995.1| N-acyl-D-glutamate deacylase [H... 150 2e-37 >gi|300309905|ref|YP_003773997.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 498 Score = 1018 bits (2633), Expect = 0.0, Method: Compositional matrix adjust. Identities = 494/498 (99%), Positives = 495/498 (99%) Query: 3 MQNSPAFDLLITGGTIIDGSKAPRFRADIGVRHGRIAEIGNLSHRQAAQRIDASGKIVAP 62 MQNSPAFDLLITGGTIIDGSKAPRFRADIGVRHGRIAEIGNLSHRQAAQRIDASGKIVAP Sbjct: 1 MQNSPAFDLLITGGTIIDGSKAPRFRADIGVRHGRIAEIGNLSHRQAAQRIDASGKIVAP 60 Query: 63 GFIDSHTHDDQAVLSQPDMTFKISQGVTTVITGNCGISIAPLRAGTPLPAPLSLLDDADG 122 GFIDSHTHDDQAVLSQPDMTFKISQGVTTVITGNCGISIAPLRAGTPLPAPLSLLDDADG Sbjct: 61 GFIDSHTHDDQAVLSQPDMTFKISQGVTTVITGNCGISIAPLRAGTPLPAPLSLLDDADG 120 Query: 123 TRFETFSSYLDALRQTPSAVNVAAMVGHSTLRVMTMDALEREARPDEISAMNALLDEALE 182 TRFETFSSYLDALRQTPSAVNVAAMVGHSTLRVMTMDALEREARPDEISAMNALLDEALE Sbjct: 121 TRFETFSSYLDALRQTPSAVNVAAMVGHSTLRVMTMDALEREARPDEISAMNALLDEALE 180 Query: 183 AGAIGISTGTYYPPAAHASTQEVIDVCRPIAGTGALYVTHMRDEADKSMEALEESFLIGR 242 AGAIGISTGTYYPPAAHASTQEVIDVCRPIAGTGALYVTHMRDEADKSMEALEESFLIGR Sbjct: 181 AGAIGISTGTYYPPAAHASTQEVIDVCRPIAGTGALYVTHMRDEADKSMEALEESFLIGR 240 Query: 243 ELNAPVVISHHKLQRAHNFGKSSQTLARIRQAMQCQCVALDCYPYNASSTMLHRDPKRLV 302 ELNAPVVISHHKLQRAHNFGKSSQTLARIRQAMQCQCVALDCYPYNASSTMLHRDPKRLV Sbjct: 241 ELNAPVVISHHKLQRAHNFGKSSQTLARIRQAMQCQCVALDCYPYNASSTMLHRDPKRLV 300 Query: 303 GRILISSSKPHPEQAGRELDAIAAEWGVSKEEAAARLQPASAVYFGMDESDVRNILAFDE 362 GRILISSSKPHPEQAGRELDAIAAEWGVSKEEAAARLQPASAVYFGMDESDVRNILAFDE Sbjct: 301 GRILISSSKPHPEQAGRELDAIAAEWGVSKEEAAARLQPASAVYFGMDESDVRNILAFDE 360 Query: 363 TMIGSDGIPTGEKPHPRLWGTFPRILGHYSRDLGLFDLEVAVWKMTGLTARNFKLHERGT 422 TMIGSDGIPTGEKPHPRLWGTFPRILGHYSRDLGLFDLEVAVWKMTGLTARNFKLHERGT Sbjct: 361 TMIGSDGIPTGEKPHPRLWGTFPRILGHYSRDLGLFDLEVAVWKMTGLTARNFKLHERGT 420 Query: 423 LEVGHYADIVVFDAATIRDTASYETPAEPAQGIDAVIVNGVVTWRLGQHMGARAGHVITR 482 LEVGHYADIVVFDAATIRDTASYETPAEPAQGIDAVIVNGVVTWRLGQHMGARAGHVI R Sbjct: 421 LEVGHYADIVVFDAATIRDTASYETPAEPAQGIDAVIVNGVVTWRLGQHMGARAGHVIAR 480 Query: 483 HAAGKRSDQAPCRSEASS 500 HAA KR DQAPCRS+ASS Sbjct: 481 HAASKRRDQAPCRSKASS 498 >gi|300311079|ref|YP_003775171.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 496 Score = 449 bits (1156), Expect = e-127, Method: Compositional matrix adjust. Identities = 239/478 (50%), Positives = 313/478 (65%), Gaps = 8/478 (1%) Query: 10 DLLITGGTIIDGSKAPRFRADIGVRHGRIAEIGNLSHRQAAQRIDASGKIVAPGFIDSHT 69 D+LI +IDGS A AD+ V GR+A IG L QAAQ IDASG++++PGFID HT Sbjct: 15 DILIRHALVIDGSGAEPVAADVAVHKGRVAAIGKLDDWQAAQEIDASGRVLSPGFIDVHT 74 Query: 70 HDDQAVLSQPDMTFKISQGVTTVITGNCGISIAPLRAGTPLPAPLSLLDDADGTRFETFS 129 HDD V+ P+M K+SQGVTTV+ GNCGIS AP+ P P++LL + + +F+ Sbjct: 75 HDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGEPPDPMNLLGKREAFHYPSFA 134 Query: 130 SYLDALRQTPSAVNVAAMVGHSTLRVMTMDALEREARPDEISAMNALLDEALEAGAIGIS 189 SY++A+ A+NVAA+VGH+ LR MD L+R A EI+ M A L EALE GA+G+S Sbjct: 135 SYVEAVNAARPAINVAALVGHTALRNNHMDRLDRAATESEIAGMRAQLREALEHGALGLS 194 Query: 190 TGTYYPPAAHASTQEVIDVCRPIAGTGALYVTHMRDEADKSMEALEESFLIGRELNAPVV 249 TG Y A +A T+EV+ + P+A G LY TH+R E ++A+EESF IG+ PVV Sbjct: 195 TGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADILDAMEESFRIGKHARVPVV 254 Query: 250 ISHHKLQRAHNFGKSSQTLARIRQAMQCQCVALDCYPYNASSTMLHRDPKRLVG--RILI 307 ISH K N+G+SS+ L + +A + Q V DCYPY ASS+ L D K++ G I + Sbjct: 255 ISHLKCAGVENWGRSSEVLHALERAARHQHVGCDCYPYTASSSTL--DLKQVTGDYDIQV 312 Query: 308 SSSKPHPEQAGRELDAIAAEWGVSKEEAAARLQPASAVYFGMDESDVRNILAFDETMIGS 367 + S+PHPEQ G+ L IAAEW V AAARL PA AVY M + DV IL+ T++GS Sbjct: 313 TWSEPHPEQGGKMLKQIAAEWDVDLHAAAARLMPAGAVYHCMSDDDVNRILSHPATVVGS 372 Query: 368 DGIPTGEKPHPRLWGTFPRILGHYSRDLGLFDLEVAVWKMTGLTARNFKLHERGTLEVGH 427 DG+P PHPRLWG FPR+LG+YSR+ LF L AV KMTGL+A+ F L RG + VG+ Sbjct: 373 DGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRFGLDLRGFVRVGY 432 Query: 428 YADIVVFDAATIRDTASYETPAEPAQGIDAVIVNGVVTWRLGQH---MGARAGHVITR 482 +AD+V+FDA T+RD AS+ P +PA GI+AV VNG + +R GQ ARAG + R Sbjct: 433 HADLVLFDAETVRDAASFTDPMQPAHGIEAVWVNGELAYR-GQDKKATAARAGRFLAR 489 >gi|300309903|ref|YP_003773995.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 150 bits (380), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 147/526 (27%), Positives = 224/526 (42%), Gaps = 85/526 (16%) Query: 9 FDLLITGGTIIDGSKAPRFRADIGVRHGRIAEIG--NLSHRQAAQRIDASGKIVAPGFID 66 DL+I I DG ++G+ RI +G + S A + IDA G IVAPGFID Sbjct: 48 LDLIIQSDRIYDGEHDEPLSGELGIAGDRIVYVGKADRSRFTALRVIDARGAIVAPGFID 107 Query: 67 SHTHDDQAVLS----QPDMTFKISQGVTTVITGNCGISIAPLRAGTPLPAPLSLLDDADG 122 +HTH D+ + + + + + ++QGVTT + GN G G Sbjct: 108 AHTHADRDMFTDDIKKRQVDYFLTQGVTTAVIGNDG-----------------------G 144 Query: 123 TRFETFSSYLDALRQTPSAVNVAAMVGHSTLRVMTMDALEREARPDEISAMNALLDEALE 182 +E + L + N A VG +R + +++ ++ AM AL+ + + Sbjct: 145 GSYEV-AQQAAWLSRHGIGPNAAMYVGFGPIRRSQLGEADQQPDQKQLEAMKALVAKGMC 203 Query: 183 AGAIGISTGTYYPPAAHASTQEVIDVCRPIAGTGALYVTHMRDEADKSM---EALEESFL 239 GAIG STG +Y P AST EVI + + A G +Y +H+RDEA ++ E++ E Sbjct: 204 EGAIGFSTGLFYAPQNFASTGEVIALAKVAAQYGGIYDSHIRDEASYNVGLAESIREVIR 263 Query: 240 IGRELNAPVVISHHKLQRAHNFGKS----SQTLARIRQAMQCQCVAL--DCYPYNASST- 292 I RE ++H K GKS S + R+ +A + Q + + D YP+ ASST Sbjct: 264 IAREAGIAANVAHIKA-----LGKSVEGQSGAIIRMIEAARAQGLRITADQYPWLASSTR 318 Query: 293 -----------------MLHR--DPK------RLVGRIL--------ISSSKPHPEQAGR 319 ML R DP R +G L I S+ +P+ G+ Sbjct: 319 FSAALIPPWALDGGRPAMLRRFDDPAQQQRLHRDIGENLRLRNGAGAILFSQGNPKYVGK 378 Query: 320 ELDAIAAEWGVSKEEAAARLQPASAVYFGMDESDVRNILAFDE---TMIGSDGIPTGEKP 376 L+ +A E AA + S + ++++AF + M SD Sbjct: 379 TLEQLAQERNTDPVSAAIEVLRDSEIVIASFNQSNKDVVAFMKRPWVMTSSDSF----HG 434 Query: 377 HPRLWGTFPRILGHYSRDLGLFDLEVAVWKMTGLTARNFKLHERGTLEVGHYADIVVFDA 436 HPR +F Y R+ L + T A + RG L+ GH+AD+VVFD Sbjct: 435 HPRTVASFAEKYHEYVRNQHTITLAQFIRSSTSYPADALGIARRGRLQPGHFADVVVFDP 494 Query: 437 ATIRDTASYETPAEPAQGIDAVIVNGVVTWRLGQHMGARAGHVITR 482 A+Y E + G+ A +VNG + G+ A G + R Sbjct: 495 IAYAPRATYVNNEERSVGVVATVVNGELVVDEGRLTNAMPGRPLLR 540 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,606,889 Number of extensions: 68940 Number of successful extensions: 233 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 230 Number of HSP's successfully gapped: 5 Length of query: 500 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 411 Effective length of database: 1,198,455 Effective search space: 492565005 Effective search space used: 492565005 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)