BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0087.p (550 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309903|ref|YP_003773995.1| N-acyl-D-glutamate deacylase [H... 1122 0.0 gi|300311079|ref|YP_003775171.1| N-acyl-D-glutamate deacylase [H... 168 1e-42 gi|300309905|ref|YP_003773997.1| N-acyl-D-glutamate deacylase [H... 148 1e-36 >gi|300309903|ref|YP_003773995.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 1122 bits (2903), Expect = 0.0, Method: Compositional matrix adjust. Identities = 548/550 (99%), Positives = 549/550 (99%) Query: 1 MPGRNHSPASRTALPGKAGQQHLRKMAIATVAACFATSGVAIAQPVQLDLIIQSDRIYDG 60 MPGRNHSPASRTALPGKAGQQHLRKMAIATVAACFATSGVAIAQPVQLDLIIQSDRIYDG Sbjct: 1 MPGRNHSPASRTALPGKAGQQHLRKMAIATVAACFATSGVAIAQPVQLDLIIQSDRIYDG 60 Query: 61 EHDEPLSGELGIAGDRIVYVGKADRSRFTALRVIDARGAIVAPGFIDAHTHADRDMFTDD 120 EHDEPLSGELGIAGDRIVYVGKADRSRFTALRVIDARGAIVAPGFIDAHTHADRDMFTDD Sbjct: 61 EHDEPLSGELGIAGDRIVYVGKADRSRFTALRVIDARGAIVAPGFIDAHTHADRDMFTDD 120 Query: 121 IKKRQVDYFLTQGVTTAVIGNDGGGSYEVAQQAAWLSRHGIGPNAAMYVGFGPIRRSQLG 180 IKKRQVDYFLTQGVTTAVIGNDGGGSYEVAQQAAWLSRHGIGPNAAMYVGFGPIRRSQLG Sbjct: 121 IKKRQVDYFLTQGVTTAVIGNDGGGSYEVAQQAAWLSRHGIGPNAAMYVGFGPIRRSQLG 180 Query: 181 EADQQPDQKQLEAMKALVAKGMCEGAIGFSTGLFYAPQNFASTGEVIALAKVAAQYGGIY 240 EADQQPDQKQLEAMKALVAKGMCEGAIGFSTGLFYAPQNFASTGEVIALAKVAAQYGGIY Sbjct: 181 EADQQPDQKQLEAMKALVAKGMCEGAIGFSTGLFYAPQNFASTGEVIALAKVAAQYGGIY 240 Query: 241 DSHIRDEASYNVGLAESIREVIRIAREAGIAANVAHIKALGKSVEGQSGAIIRMIEAARA 300 DSHIRDEASYNVGLAESIREVIRIAREAGIAANVAHIKALGKSVEGQSGAIIRMIEAARA Sbjct: 241 DSHIRDEASYNVGLAESIREVIRIAREAGIAANVAHIKALGKSVEGQSGAIIRMIEAARA 300 Query: 301 QGLRITADQYPWLASSTRFSAALIPPWALDGGRPAMLRRFDDPAQQQRLHRDIGENLRLR 360 QGLRITADQYPWLASSTRFSAALIPPWALDGGRPAMLRRFDDPAQQQRLHRDIGENLRLR Sbjct: 301 QGLRITADQYPWLASSTRFSAALIPPWALDGGRPAMLRRFDDPAQQQRLHRDIGENLRLR 360 Query: 361 NGAGAILFSQGNPKYVGKTLEQIAQERNTDPVSAAIEVLRDSEIVIASFNQSNKDVVAFM 420 NGAGAILFSQGNPKYVGKTLEQ+AQERNTDPVSAAIEVLRDSEIVIASFNQSNKDVVAFM Sbjct: 361 NGAGAILFSQGNPKYVGKTLEQLAQERNTDPVSAAIEVLRDSEIVIASFNQSNKDVVAFM 420 Query: 421 KRPWVMTSSDSFHGHPRTVASFAEKYHEYVLNQHTITLAQFIRSSTSYPADALGIARRGR 480 KRPWVMTSSDSFHGHPRTVASFAEKYHEYV NQHTITLAQFIRSSTSYPADALGIARRGR Sbjct: 421 KRPWVMTSSDSFHGHPRTVASFAEKYHEYVRNQHTITLAQFIRSSTSYPADALGIARRGR 480 Query: 481 LQPGHFADVVVFDPIAYAPRATYVNNEERSVGVVATVVNGELVVDEGRLTNAMPGRPLLR 540 LQPGHFADVVVFDPIAYAPRATYVNNEERSVGVVATVVNGELVVDEGRLTNAMPGRPLLR Sbjct: 481 LQPGHFADVVVFDPIAYAPRATYVNNEERSVGVVATVVNGELVVDEGRLTNAMPGRPLLR 540 Query: 541 TPKQGACPQS 550 TPKQGACPQS Sbjct: 541 TPKQGACPQS 550 >gi|300311079|ref|YP_003775171.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 496 Score = 168 bits (426), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 145/531 (27%), Positives = 241/531 (45%), Gaps = 83/531 (15%) Query: 47 QLDLIIQSDRIYDGEHDEPLSGELGIAGDRIVYVGKADRSRFTALRVIDARGAIVAPGFI 106 + D++I+ + DG EP++ ++ + R+ +GK D + A + IDA G +++PGFI Sbjct: 13 RCDILIRHALVIDGSGAEPVAADVAVHKGRVAAIGKLDD--WQAAQEIDASGRVLSPGFI 70 Query: 107 DAHTHADRDMFTDDIKKRQVDYFLTQGVTTAVIGNDGGG--------------------- 145 D HTH D T+ I+ ++ L+QGVTT V+GN G Sbjct: 71 DVHTHDD----TNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGEPPDPMNLLGKRE 126 Query: 146 -----SYEVAQQAAWLSRHGIGPNAAMYVGFGPIRRSQLGEADQQPDQKQLEAMKALVAK 200 S+ +A +R I N A VG +R + + D+ + ++ M+A + + Sbjct: 127 AFHYPSFASYVEAVNAARPAI--NVAALVGHTALRNNHMDRLDRAATESEIAGMRAQLRE 184 Query: 201 GMCEGAIGFSTGLFYAPQNFASTGEVIALAKVAAQYGGIYDSHIRDEASYNVGLAESIRE 260 + GA+G STGL Y A T EV+ALA+ A++GG+Y +H+R E + + +++ E Sbjct: 185 ALEHGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFA---DILDAMEE 241 Query: 261 VIRIAREAGIAANVAHIKALGKSVEGQSGAIIRMIE-AARAQGLRITADQYPWLASSTRF 319 RI + A + ++H+K G G+S ++ +E AAR Q + D YP+ ASS+ Sbjct: 242 SFRIGKHARVPVVISHLKCAGVENWGRSSEVLHALERAARHQ--HVGCDCYPYTASSSTL 299 Query: 320 SAALIPPWALDGGRPAMLRRFDDPAQQQRLHRDIGENLRLRNGAGAILFSQGNPKYVGKT 379 +Q DI + +S+ +P+ GK Sbjct: 300 D-----------------------LKQVTGDYDI-----------QVTWSEPHPEQGGKM 325 Query: 380 LEQIAQERNTDPVSAAIEVLRDSEIVIASFNQSNKDVVAFMKRPWVMTSSDSFHG----H 435 L+QIA E + D +AA ++ + S+ DV + P + SD H Sbjct: 326 LKQIAAEWDVDLHAAAARLMPAGAVY---HCMSDDDVNRILSHPATVVGSDGLPNDPLPH 382 Query: 436 PRTVASFAEKYHEYVLNQHTITLAQFIRSSTSYPADALGIARRGRLQPGHFADVVVFDPI 495 PR +F Y Q +LA + T A G+ RG ++ G+ AD+V+FD Sbjct: 383 PRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRFGLDLRGFVRVGYHADLVLFDAE 442 Query: 496 AYAPRATYVNNEERSVGVVATVVNGELVV--DEGRLTNAMPGRPLLRTPKQ 544 A++ + + + G+ A VNGEL + + T A GR L RT ++ Sbjct: 443 TVRDAASFTDPMQPAHGIEAVWVNGELAYRGQDKKATAARAGRFLARTVQK 493 >gi|300309905|ref|YP_003773997.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 498 Score = 148 bits (373), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 147/526 (27%), Positives = 223/526 (42%), Gaps = 85/526 (16%) Query: 48 LDLIIQSDRIYDGEHDEPLSGELGIAGDRIVYVGKADRSRFTALRVIDARGAIVAPGFID 107 DL+I I DG ++G+ RI +G + S A + IDA G IVAPGFID Sbjct: 7 FDLLITGGTIIDGSKAPRFRADIGVRHGRIAEIG--NLSHRQAAQRIDASGKIVAPGFID 64 Query: 108 AHTHADRDMFTDDIKKRQVDYFLTQGVTTAVIGNDG-----------------------G 144 +HTH D+ + + + + + ++QGVTT + GN G G Sbjct: 65 SHTHDDQAVLS----QPDMTFKISQGVTTVITGNCGISIAPLRAGTPLPAPLSLLDDADG 120 Query: 145 GSYEV-AQQAAWLSRHGIGPNAAMYVGFGPIRRSQLGEADQQPDQKQLEAMKALVAKGMC 203 +E + L + N A VG +R + +++ ++ AM AL+ + + Sbjct: 121 TRFETFSSYLDALRQTPSAVNVAAMVGHSTLRVMTMDALEREARPDEISAMNALLDEALE 180 Query: 204 EGAIGFSTGLFYAPQNFASTGEVIALAKVAAQYGGIYDSHIRDEASYNVGLAESIREVIR 263 GAIG STG +Y P AST EVI + + A G +Y +H+RDEA ++ E++ E Sbjct: 181 AGAIGISTGTYYPPAAHASTQEVIDVCRPIAGTGALYVTHMRDEADKSM---EALEESFL 237 Query: 264 IAREAGIAANVAHIKA-----LGKSVEGQSGAIIRMIEAARAQGLRITADQYPWLASSTR 318 I RE ++H K GKS S + R+ +A + Q + + D YP+ ASST Sbjct: 238 IGRELNAPVVISHHKLQRAHNFGKS----SQTLARIRQAMQCQCVAL--DCYPYNASST- 290 Query: 319 FSAALIPPWALDGGRPAMLRRFDDPAQQQRLHRDIGENLRLRNGAGAILFSQGNPKYVGK 378 ML R DP R +G L I S+ +P+ G+ Sbjct: 291 -----------------MLHR--DP------KRLVGRIL--------ISSSKPHPEQAGR 317 Query: 379 TLEQIAQERNTDPVSAAIEVLRDSEIVIASFNQSNKDVVAFMKRPWVMTSSDSF----HG 434 L+ IA E AA + S + ++++AF + M SD Sbjct: 318 ELDAIAAEWGVSKEEAAARLQPASAVYFGMDESDVRNILAFDE---TMIGSDGIPTGEKP 374 Query: 435 HPRTVASFAEKYHEYVLNQHTITLAQFIRSSTSYPADALGIARRGRLQPGHFADVVVFDP 494 HPR +F Y + L + T A + RG L+ GH+AD+VVFD Sbjct: 375 HPRLWGTFPRILGHYSRDLGLFDLEVAVWKMTGLTARNFKLHERGTLEVGHYADIVVFDA 434 Query: 495 IAYAPRATYVNNEERSVGVVATVVNGELVVDEGRLTNAMPGRPLLR 540 A+Y E + G+ A +VNG + G+ A G + R Sbjct: 435 ATIRDTASYETPAEPAQGIDAVIVNGVVTWRLGQHMGARAGHVIAR 480 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,810,249 Number of extensions: 79175 Number of successful extensions: 301 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 297 Number of HSP's successfully gapped: 7 Length of query: 550 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 460 Effective length of database: 1,193,720 Effective search space: 549111200 Effective search space used: 549111200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)