BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0082.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 780 0.0 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 87 3e-18 gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 84 2e-17 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 72 8e-14 gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 69 8e-13 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 780 bits (2015), Expect = 0.0, Method: Compositional matrix adjust. Identities = 382/386 (98%), Positives = 386/386 (100%) Query: 1 MRALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYRGKIPATKAGDILGH 60 MRALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYRGKIPATKAGDILGH Sbjct: 1 MRALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYRGKIPATKAGDILGH 60 Query: 61 EFMGVVVEAGPAVKRLKKGDRVVVPFVIACGQCFFCDRHLYAACETTNPDRGALMNAKGI 120 EFMGVVVEAGPA+KRLKKGDRVVVPFVIACGQCFFCD+HLYAACETTNPDRGALMNAKGI Sbjct: 61 EFMGVVVEAGPAIKRLKKGDRVVVPFVIACGQCFFCDQHLYAACETTNPDRGALMNAKGI 120 Query: 121 RSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVK 180 RSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVK Sbjct: 121 RSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVK 180 Query: 181 NAGVREGASVAIYGAGPVGQMAAASARMLGAEKIFMVDHHAYRLRFAQEQYDVIPVNFDQ 240 NAGVREG+SVAIYGAGPVGQMAAASARMLGAE+IFMVDHHAYRLRFAQEQYDVIPVNFDQ Sbjct: 181 NAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVDHHAYRLRFAQEQYDVIPVNFDQ 240 Query: 241 VDPAEYIIANTAGRGVDGVIDAVGFEAKGSVLETAMTTLKLEGSSGQALRHCIASVRRGG 300 VDPAEYIIANTAGRGVDGVIDAVGFEAKGSVLETAMTTLKLEGSSGQALRHCIASVRRGG Sbjct: 241 VDPAEYIIANTAGRGVDGVIDAVGFEAKGSVLETAMTTLKLEGSSGQALRHCIASVRRGG 300 Query: 301 VISVPGVYAGFIHGFLFGDIFEKGVTVKTGQTHVQHYMPELLASIEEGRLRPDAIITHRL 360 VISVPGVYAGFIHGFLFGDIFEKGVTVKTGQTHVQHYMPELLASIEEGRLRPDAIITHRL Sbjct: 301 VISVPGVYAGFIHGFLFGDIFEKGVTVKTGQTHVQHYMPELLASIEEGRLRPDAIITHRL 360 Query: 361 PLEDAARGYQIFNDKLEDCRKVVLTA 386 PLEDAARGYQIFNDKLEDCRKVVLTA Sbjct: 361 PLEDAARGYQIFNDKLEDCRKVVLTA 386 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 86.7 bits (213), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 102/354 (28%), Positives = 142/354 (40%), Gaps = 42/354 (11%) Query: 27 DVILKVTATAICGSDLHLYRGKIPATKAGDILGHEFMGVVVEAGPAVKRLKKGDRVVVPF 86 +V++++ A +C SDL + G P LGHE VVE G V L+ GD VV+ F Sbjct: 37 EVLVRMKAAGLCHSDLSVITGVRP-RPVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVF 95 Query: 87 VIACGQCFFCDRHLYAACE---TTNPDRGALMNAKGIRSGAA----MFGYSHLYGGMPGG 139 V +CG C C A CE TN L + +R G S Sbjct: 96 VPSCGHCLPCMEGRPALCEPGAATNTVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVS 155 Query: 140 QAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVKNAGVREGASVAIYGAGPVG 199 + V++ +AD+ P+ + LF +L AV A V+ G + A+ G G VG Sbjct: 156 RRSCVKI-EADIDPV--------QAALFGCAVLTGVGAAVNTAEVKAGTTAAVLGLGGVG 206 Query: 200 QMAAASARMLGAEKIFMVDHHAYRLRFAQEQYDVIPVNFDQVDPAEYIIANTAGRGVDGV 259 A A GA ++ VD H +L A+ VN D E + A T G GVD Sbjct: 207 LCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDAVEKVRALTRG-GVD-- 263 Query: 260 IDAVGFEAKGSVLETAMTTLKLEGSSGQALRHCIASVRRGGVISVPGVYAGFIHGFLFG- 318 FE GSV QA+ RRGG+ G+ A H L Sbjct: 264 ---YAFEMAGSV---------------QAMEAAYRMTRRGGMTVTAGLPAPGQHWALQQV 305 Query: 319 DIFEKGVTVK---TGQTHVQHYMPELLASIEEGRLRPDAIITHRLPLEDAARGY 369 + + TVK G +P + + G+L D ++ RL L D RG+ Sbjct: 306 SLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGF 359 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 83.6 bits (205), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 109/369 (29%), Positives = 162/369 (43%), Gaps = 37/369 (10%) Query: 24 EADDVILKVTATAICGSDLHLYRGKIPATKAGDILGHEFMGVVVEAGPAVKRLKKGDRVV 83 +A +V++++ AT IC +D + G P ILGHE GVVV+ GP VK LKK D V+ Sbjct: 26 KAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVKSLKKDDHVI 85 Query: 84 VPFVIACGQCFFCDRHLYAACETTNPDRG-ALMNAKGIR---SGAAMFGYSHLYGGMPGG 139 + C +C FC C+ +G LM R G +F Y Sbjct: 86 PLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPDATSRFSLDGKPLFHYMGT-----ST 140 Query: 140 QAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVK-NAGVREGASVAIYGAGPV 198 + Y+ VP+ + IR D+ ++V ++ + TG AV A V GA+V ++G G + Sbjct: 141 FSNYIVVPEIALAKIRSDAPF--DKVCYIGCGVTTGVGAVLFTAKVEAGANVVVFGLGGI 198 Query: 199 GQMAAASARMLGAEKIFMVDHHAYRLRFAQEQYDVIPVNFDQV-DPAEYIIANTAGRGVD 257 G +A+M+GA KI VD + R A++ +N V + + II T G G D Sbjct: 199 GLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKDVPNVVDAIIGMTDG-GAD 257 Query: 258 GVIDAVGFEAKGSVLETAMTTLKLEGSSGQALRHCIASVRRGGVISVPG---VYAGFIHG 314 FE G+ + GQ++ +A+ G IS V G Sbjct: 258 -----YSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAA--GQEISTRPFQLVTGRVWKG 310 Query: 315 FLFGDIFEKGVTVKTGQTHVQHYMPELLASIEEGRLRPDAIITHRLPLEDAARGYQIFND 374 FG G+T V P+++ EG+L D +ITH+LPLE G+ + Sbjct: 311 SAFGG--------ARGRTDV----PKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKS 358 Query: 375 KLEDCRKVV 383 E R VV Sbjct: 359 G-ESIRSVV 366 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 72.0 bits (175), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 97/386 (25%), Positives = 151/386 (39%), Gaps = 66/386 (17%) Query: 2 RALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYRGK---IPATKAGDIL 58 + L+ H+ HD+R++ + +Q + +KV A ICGSDLH Y + K +L Sbjct: 3 QGLVIHAPHDLRIQALDTGALQP-QQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVL 61 Query: 59 GHEFMGVVVEAGPAVKRLKKGDRVVVPFVIACGQCFFC---------DRHLYAACETTNP 109 GHE G + E G AV + G R+ + CG C +C D Y + T Sbjct: 62 GHEVSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPH 121 Query: 110 DRGALMNAKGIRSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLS 169 +GA I A H+ G ++A+A+ Sbjct: 122 VQGAFRQEIIIERSQAHVVADHVSDG---------------------EAAMAEP------ 154 Query: 170 DILPTGYQAVKNAGVREGASVAIYGAGPVGQMAAASARMLGAEKIFMVDHHAYRLRFAQE 229 L AV+ AG G V + G GP+G + +AR GA ++ + D + L+ A + Sbjct: 155 --LSVALHAVRRAGPLVGKRVLVTGCGPIGALVVVAARRAGALEVVVTDVASMPLQAALQ 212 Query: 230 QYDVIPVNFDQVDPAEYIIANTAGRGVDGVIDAVGFEAKGSVLETAMTTLKLEGSSGQAL 289 +N + A + A A +G V+ FEA G+ E A+ LE QA+ Sbjct: 213 VGADSAINMAETPAA--LKAYAADKGTFDVL----FEASGN--ERALVG-ALEALRPQAI 263 Query: 290 RHCIASVRRGGVISVPGVYAGFIHGFLFGDIFEKGVTVKTGQTHVQHYMPELLASIEEGR 349 I V GG I P L G E + H + M L + + Sbjct: 264 ---IVQVGLGGDIQFPA-------NLLVGKELEWRGAFR---FHEEFAMAVELMNRKLVD 310 Query: 350 LRPDAIITHRLPLEDAARGYQIFNDK 375 ++P +IT P+ A ++I D+ Sbjct: 311 VKP--LITATYPIAQAVEAFEIAGDR 334 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 68.6 bits (166), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 74/267 (27%), Positives = 115/267 (43%), Gaps = 27/267 (10%) Query: 1 MRALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLY-RGKIPATK--AGDI 57 M+AL+ + ++++ + P A DV +++ ICGSDLH Y G I K A + Sbjct: 1 MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV 60 Query: 58 LGHEFMGVVVEAGPAVKRLKKGDRVVVPFVIACGQCFFCDRHLYAACETTNPDRGALMNA 117 LGHE G V+E G AV LK GDRV C ++ RG Sbjct: 61 LGHEASGTVIEVGSAVSHLKVGDRV-------------CMEPGIPRLDSPATLRGMYNLD 107 Query: 118 KGIRSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQ 177 +R A + L G + A R+P + + A+ ++ + L G Q Sbjct: 108 PAVRFWATPPIHGCLTGSVVHPAAFTYRLPD--------NVSFAEGAIV---EPLSIGLQ 156 Query: 178 AVKNAGVREGASVAIYGAGPVGQMAAASARMLGAEKIFMVDHHAYRLRFAQEQYDVIPVN 237 A A ++ G + + GAG +G M A +A GA ++ + D A +L + VI V+ Sbjct: 157 AATKARMKPGDTAVVIGAGTIGAMTALAALAGGAARVILADVVAEKLAHFADNPAVITVD 216 Query: 238 FDQVDPAEYIIANTAGRGVDGVIDAVG 264 + + + T G G D V +A G Sbjct: 217 VTRETLTDVVRQATDGWGADVVFEASG 243 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,277,578 Number of extensions: 57062 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 7 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)