BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0062.p (586 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hy... 1140 0.0 gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hy... 599 e-172 gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ... 106 4e-24 >gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 589 Score = 1140 bits (2950), Expect = 0.0, Method: Compositional matrix adjust. Identities = 570/572 (99%), Positives = 571/572 (99%) Query: 1 MNQNPAIDYQQQRFAWTPCAEQQSPDHAPVHPVVIIGAGPVGLACAIDLARQGIAVVLVD 60 MNQNPAIDYQQQRFAWTPCAEQQSP HAPVHPVVIIGAGPVGLACAIDLARQGIAVVLVD Sbjct: 1 MNQNPAIDYQQQRFAWTPCAEQQSPGHAPVHPVVIIGAGPVGLACAIDLARQGIAVVLVD 60 Query: 61 DDDTLSTGSRAICFAKRTLDIFDRIGCGQRMVDKGVGWNRGKVFLRDQAVYEFDLLPESG 120 DDDTLSTGSRAICFAKRTLDIFDRIGCGQRMVDKGVGWNRGKVFLRDQAVYEFDLLPESG Sbjct: 61 DDDTLSTGSRAICFAKRTLDIFDRIGCGQRMVDKGVGWNRGKVFLRDQAVYEFDLLPESG 120 Query: 121 HARPAFINLQQYYVEGYLLEQAQQWPHIEIRWRNKVSAIAHHEDHEALTVDTPQGSYTLK 180 HARPAFINLQQYYVEGYLLEQAQQWPHIEIRW+NKVSAIAHHEDHEALTVDTPQGSYTLK Sbjct: 121 HARPAFINLQQYYVEGYLLEQAQQWPHIEIRWKNKVSAIAHHEDHEALTVDTPQGSYTLK 180 Query: 181 ARYVVAADGSRSPLRKMMGLESQGHTFRDRFLIADVKMDAPFPAERWFWFDPPFHPQQSV 240 ARYVVAADGSRSPLRKMMGLESQGHTFRDRFLIADVKMDAPFPAERWFWFDPPFHPQQSV Sbjct: 181 ARYVVAADGSRSPLRKMMGLESQGHTFRDRFLIADVKMDAPFPAERWFWFDPPFHPQQSV 240 Query: 241 LLHRQPDDVWRIDFQLGWDADPVLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRM 300 LLHRQPDDVWRIDFQLGWDADPVLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRM Sbjct: 241 LLHRQPDDVWRIDFQLGWDADPVLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRM 300 Query: 301 ERFRHGRVLFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASER 360 ERFRHGRVLFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASER Sbjct: 301 ERFRHGRVLFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASER 360 Query: 361 EYAADENLLNSTRSTDFITPKSAVSRAFRDAVLGLAREHAFARRLVNSGRLSLPATLDQS 420 EYAADENLLNSTRSTDFITPKSAVSRAFRDAVLGLAREHAFARRLVNSGRLSLPATLDQS Sbjct: 361 EYAADENLLNSTRSTDFITPKSAVSRAFRDAVLGLAREHAFARRLVNSGRLSLPATLDQS 420 Query: 421 PLNTPDQAAAPEAVAFTGPMRPGAPCCDAPLLQAGQPAWLLPQLGDRFVLMIFAAPAQLP 480 PLNTPDQAAAPEAVAFTGPMRPGAPCCDAPLLQAGQPAWLLPQLGDRFVLMIFAAPAQLP Sbjct: 421 PLNTPDQAAAPEAVAFTGPMRPGAPCCDAPLLQAGQPAWLLPQLGDRFVLMIFAAPAQLP 480 Query: 481 AATRQALAALAQPDALRLLFISAQAATQEDERVLQTLAGQAIQVQDIDTLAAQRYDAHPG 540 AATRQALAALAQPDALRLLFISAQAATQEDERVLQTLAGQAIQVQDIDTLAAQRYDAHPG Sbjct: 481 AATRQALAALAQPDALRLLFISAQAATQEDERVLQTLAGQAIQVQDIDTLAAQRYDAHPG 540 Query: 541 SCYLIRPDQHVSARWRSADAAAVAQALLRATG 572 SCYLIRPDQHVSARWRSADAAAVAQALLRATG Sbjct: 541 SCYLIRPDQHVSARWRSADAAAVAQALLRATG 572 >gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 547 Score = 599 bits (1544), Expect = e-172, Method: Compositional matrix adjust. Identities = 307/565 (54%), Positives = 381/565 (67%), Gaps = 28/565 (4%) Query: 9 YQQQRFAWTPCAEQQSPDHAPVHPVVIIGAGPVGLACAIDLARQGIAVVLVDDDDTLSTG 68 Y RFA+ EQ+ HPVV+IGAGP+GL A++ A +G+ VV++DD+DT+S G Sbjct: 5 YTYPRFAYRQSDEQREA-RPSRHPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTVSIG 63 Query: 69 SRAICFAKRTLDIFDRIGCGQRMVDKGVGWNRGKVFLRDQAVYEFDLLPESGHARPAFIN 128 SRA+C+AKR L+I+DR+G RMV+KGV W GKVF ++ VY FDLLPE H PA IN Sbjct: 64 SRAVCYAKRPLEIWDRLGVAPRMVEKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPAMIN 123 Query: 129 LQQYYVEGYLLEQAQQWPHIEIRWRNKVSAIAHHEDHEALTVDTPQGSYTLKARYVVAAD 188 LQQYY+E YL+E Q P I++RW++K+ ++ + H L V+TP G + ++A +V+A D Sbjct: 124 LQQYYLEQYLVEACQAQPLIDLRWKHKMISLHQDDTHATLDVETPDGIFRMEADWVIACD 183 Query: 189 GSRSPLRKMMGLESQGHTFRDRFLIADVKMDAPFPAERWFWFDPPFHPQQSVLLHRQPDD 248 G+ S RKM+G E G F+DRFLIADV M A FPAERWFWFDPPFH QSVLLH+Q D+ Sbjct: 184 GANSDTRKMVGAEFSGQFFQDRFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQCDN 243 Query: 249 VWRIDFQLGWDADPVLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRMERFRHGRV 308 VWRIDFQLGW+ADP EK PE+VIPR++A+LG+EV F LEWVSVY F+C R+++ R+ RV Sbjct: 244 VWRIDFQLGWEADPEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRYQRV 303 Query: 309 LFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASEREYAADENL 368 LF GDAAH VSPFGARGAN+G+QD +NL WKL V+ GKAP S +DSY ER +AAD+NL Sbjct: 304 LFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAPPSFIDSYHEERAWAADDNL 363 Query: 369 LNSTRSTDFITPKSAVSRAFRDAVLGLAREHAFARRLVNSGRLSLPATLDQSPLNTPDQA 428 STRSTDFITPKS S+A RDAVLGLA FAR LVNSGRLS P + SPLNT D Sbjct: 364 RQSTRSTDFITPKSRASKALRDAVLGLAETEEFARTLVNSGRLSTPTSYVHSPLNTAD-- 421 Query: 429 AAPEAVAFTGPMRPGAPCCDAPLLQAGQPAWLLPQLGDRFVLMIFAAPAQLPAATRQALA 488 A F G M PG C DAP+L+ GQP WLL LG FV++ F A A P A +A Sbjct: 422 ----AALFGGRMAPGTVCADAPVLREGQPGWLLESLGAGFVVLFFGASA--PQAVIEAAG 475 Query: 489 ALAQPDALRLLFISAQAATQEDERVLQTLAGQAIQVQDIDTLAAQRYDAHPGSCYLIRPD 548 ISA+ T G ++ D + LAA RY A G YL+RPD Sbjct: 476 ------------ISARVVT-------VGAPGSGAELIDREGLAALRYAASEGCVYLVRPD 516 Query: 549 QHVSARWRSADAAAVAQALLRATGH 573 QHV+ARW + +A AL R G Sbjct: 517 QHVAARWNRFHSDDIAAALARCVGQ 541 >gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate hydroxylase [Herbaspirillum seropedicae SmR1] Length = 544 Score = 106 bits (265), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 97/338 (28%), Positives = 160/338 (47%), Gaps = 22/338 (6%) Query: 33 VVIIGAGPVGLACAIDLARQGIAVVLVDDDDTLSTGSRAICFAKRTLDIFDRIGCGQRMV 92 V+I+GAGPVG+ L GI+ +L++ + + RA+ L F G +V Sbjct: 19 VLIVGAGPVGVTLGNLLGTYGISTLLIERNLDVLDYPRAVGIDDEALRTFQSAGMADAIV 78 Query: 93 DKGVGWNRGKVFLRDQAVYEFDLLP---ESGHARPAFINLQQYYVEGYLLEQAQQWPHIE 149 + +++ D+ + ++ P E G R + Q E L +++ H+ Sbjct: 79 RDVIQNVPMRMYTADRRCFA-EIQPTTREFGWYRRNLFS--QPLGEATLRLGLRRFEHVS 135 Query: 150 IRWRNKVSAIAHHE-DHEALTVDTPQGSYTLKARYVVAADGSRSPLR-KMMGLESQGHTF 207 +R ++ + E +A +D TL+A+YVV DG RS +R K++ L +G T Sbjct: 136 LRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGGRSTVREKILKLPYEGKTH 195 Query: 208 RDRFLIADVK---MDAPFPAERWFWFDPPFHPQQSVLLHRQPDDVWRIDFQL--GWDADP 262 ++++ + +DAP+ A PQ+ + P V R +F L G D + Sbjct: 196 PAKWVVIECDGDPLDAPYTALH-------CEPQRPYVCLALPYGVRRWEFMLFPGEDGEQ 248 Query: 263 VLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRMERFRHGRVLFVGDAAHCVSPFG 322 +L PE+V +R + + + ++ VYT + GR+ GDAAH P+ Sbjct: 249 ML--APEKVRELLREHVRDVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHITPPWI 306 Query: 323 ARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASER 360 +G N+G++DA NLAWKLA VLQG+ LL SY ER Sbjct: 307 GQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEER 344 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,799,556 Number of extensions: 77792 Number of successful extensions: 294 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 290 Number of HSP's successfully gapped: 3 Length of query: 586 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 496 Effective length of database: 1,193,720 Effective search space: 592085120 Effective search space used: 592085120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)