BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0060.p (450 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 881 0.0 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 360 e-100 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 353 2e-98 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 334 8e-93 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 298 8e-82 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 881 bits (2276), Expect = 0.0, Method: Compositional matrix adjust. Identities = 445/449 (99%), Positives = 446/449 (99%) Query: 2 MTPSPGGATLSATPSLASQAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQD 61 MTPS GGATLS TPSLASQAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQD Sbjct: 1 MTPSSGGATLSTTPSLASQAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQD 60 Query: 62 LGFSDAMYGLGAAIFYVGYVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAA 121 LGFSDAMYGLGAAIFYVGYVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAA Sbjct: 61 LGFSDAMYGLGAAIFYVGYVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAA 120 Query: 122 HFYTLRFLLGVFEAGFFPGIVLYLSYWFLARRRAAVMAIFFAGVAVAGVLGGLVSGWIMR 181 HFYTLRFLLGVFEAGFFPGIVLYLSYWF ARRRAAVMAIFFAGVAVAGVLGGLVSGWIMR Sbjct: 121 HFYTLRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMR 180 Query: 182 DMAGVMGLYGWQWMFAIEGAPAVLLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEAL 241 DMAGVMGLYGWQWMFAIEGAPAV+LGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEAL Sbjct: 181 DMAGVMGLYGWQWMFAIEGAPAVVLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEAL 240 Query: 242 RTHHVSGHSLAALRQALSHRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSL 301 RTHHVSGHSLAALRQALSHRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSL Sbjct: 241 RTHHVSGHSLAALRQALSHRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSL 300 Query: 302 YSVIPNAIGAVGLILIARRSDRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLSV 361 YSVIPNAIGAVGLILIARRSDRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLSV Sbjct: 301 YSVIPNAIGAVGLILIARRSDRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLSV 360 Query: 362 AAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNMDIA 421 AAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNMDIA Sbjct: 361 AAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNMDIA 420 Query: 422 LYILAGLLLLSGLAMWSLIPRKAATAATA 450 LYILAGLLLLSGLAMWSLIPRKAATAATA Sbjct: 421 LYILAGLLLLSGLAMWSLIPRKAATAATA 449 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 360 bits (924), Expect = e-100, Method: Compositional matrix adjust. Identities = 186/413 (45%), Positives = 261/413 (63%), Gaps = 6/413 (1%) Query: 22 EDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAIFYVGYV 81 EDA Y KV R++P LF+CYV S+LDR+N+GFA+LQM QDL FSD +YGLGA IF++GY Sbjct: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 Query: 82 LFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAAHFYTLRFLLGVFEAGFFPGI 141 +FEVPSNM L + GAR +RIM+ WG+ S M VT FY +RFLLGV EAGFFPG+ Sbjct: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 Query: 142 VLYLSYWFLARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGA 201 +LYL+YW+ A RR + A+F G+A++GV+GG +SGWIM M GV G GWQWMF +E Sbjct: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 Query: 202 PAVLLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEALRTHHVSGHSLAALRQALSHR 261 P+++LG++ I + D A WLS EE+ L+A E + H+S L ++ Sbjct: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKEHLS------LGAMFANG 250 Query: 262 TTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSLYSVIPNAIGAVGLILIARRS 321 +L A IYF G ++FW+P +I+ G+ D + V L + IP A A ++LI R + Sbjct: 251 RVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSA 310 Query: 322 DRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLL 381 D R ER H A+ G + L T + + +++ L++A+V I LP+FWSLP L Sbjct: 311 DARRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFL 370 Query: 382 PAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNMDIALYILAGLLLLSGL 434 A GIA I+S+G +G VSP+L+G +K T + + +Y+LA L++ GL Sbjct: 371 SGTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGL 423 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 353 bits (906), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 190/441 (43%), Positives = 267/441 (60%), Gaps = 9/441 (2%) Query: 1 MMTPSPGGATLSATPSLASQAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQ 60 M T S G L +++D +Y+KV+ RI+PFL +CYV+++LDR+N+GFA+LQM Q Sbjct: 1 MTTASTTGVGLDVN----DKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQ 56 Query: 61 DLGFSDAMYGLGAAIFYVGYVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDA 120 DLGFS+ ++GLGA +F++GY LFEVPSN+ + + GAR +RIM+ WG+ S M V +A Sbjct: 57 DLGFSETVFGLGAGLFFIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNA 116 Query: 121 AHFYTLRFLLGVFEAGFFPGIVLYLSYWFLARRRAAVMAIFFAGVAVAGVLGGLVSGWIM 180 FY LRFLLG+ EAGF+PGI+LYL+YWF + RRA V+A+F +G+ VAG+LG +SGWIM Sbjct: 117 TQFYILRFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIM 176 Query: 181 RDMAGVMGLYGWQWMFAIEGAPAVLLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEA 240 GL GWQWMF IE PAVL+G+ + L + AKWL+ EE++ LQA +A Sbjct: 177 DAFHQNGGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDA 236 Query: 241 LRTHHVSGHSLAALRQALSHRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVS 300 S H A+ + +L IYF+ G L WMP +++ G+K +E+ Sbjct: 237 KGKE--SKHGFGAI---VKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIG 291 Query: 301 LYSVIPNAIGAVGLILIARRSDRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLS 360 L S IP + + LI R +DR ER H + G + L + AVS+ LS Sbjct: 292 LLSAIPFGCAIIAMNLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLS 351 Query: 361 VAAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNMDI 420 +AA + P+FWSLP L A GIA I+SVG +G VSP+LIG +K T N Sbjct: 352 LAAAGVLTCAPLFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNAT 411 Query: 421 ALYILAGLLLLSGLAMWSLIP 441 +Y+LA +L++ +A + P Sbjct: 412 GMYMLAVMLVVGSIATLATKP 432 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 334 bits (857), Expect = 8e-93, Method: Compositional matrix adjust. Identities = 178/411 (43%), Positives = 262/411 (63%), Gaps = 7/411 (1%) Query: 23 DAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAIFYVGYVL 82 + YRK+ R+IPFL +V++++DR+N+GFA+LQM QDL FS+A+YGLGA IF++GY + Sbjct: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 Query: 83 FEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAAHFYTLRFLLGVFEAGFFPGIV 142 FEVPSN+ L + GAR+T +RI +LWGLAS+ M VT FY LRFLLG+FEAGFFPG+V Sbjct: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVV 141 Query: 143 LYLSYWFLARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGAP 202 LYL+YWF A+RRA V +F A+AG +GG ++G IM M GV L WQW+F +EG P Sbjct: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIP 201 Query: 203 AVLLGLLAIFLLADGPAQAKWLSPEERSFL-QARHQQEALRTHHVSGHSLAALRQALSHR 261 ++L G + L + PA AKWLS E++ + Q + + H S + A ++ Sbjct: 202 SILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGKHFS------FKDACTNY 255 Query: 262 TTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSLYSVIPNAIGAVGLILIARRS 321 ++ A +YF + G+ T+ FW P +I++ G+K +++ L S IP G + +I S Sbjct: 256 RVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHS 315 Query: 322 DRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLL 381 D+ ER H A+ T + L A + + ++L L++AA+ I AA P+FWS+P L Sbjct: 316 DKTGERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFL 375 Query: 382 PAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNMDIALYILAGLLLLS 432 A GIA I+S+G +G V+P++IG +KT TG++ LY +A L L++ Sbjct: 376 AGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVA 426 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 298 bits (762), Expect = 8e-82, Method: Compositional matrix adjust. Identities = 173/436 (39%), Positives = 257/436 (58%), Gaps = 16/436 (3%) Query: 23 DAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAIFYVGYVL 82 D +Y+KV +I+P+L + YV+++LDR+N+GFA+L M DLG S+A YGLGA IF++GY + Sbjct: 13 DGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAGIFFLGYFI 72 Query: 83 FEVPSNMALARFGARRTFSRIMVLWGLASVGM---------MAVTDAAHFYTLRFLLGVF 133 FEVPSN+ L R GA+ +RIM+ WG+ S M + V++ FYTLRFLLG Sbjct: 73 FEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFYTLRFLLGAS 132 Query: 134 EAGFFPGIVLYLSYWFLARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQ 193 EAGFFPG++LY++YW+ A + VMA+ G + +LGG +SG +M+ M L GWQ Sbjct: 133 EAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGSGDSLAGWQ 192 Query: 194 WMFAIEGAPAVLLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEALRTHHVSGHSLAA 253 WMF IE PA LLG++ +F+L++ A AKWL+PEE++ L R E +V SL A Sbjct: 193 WMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEKVNVPLKSLYA 252 Query: 254 LRQALSHRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSLYSVIPNAIGAVG 313 L + A +Y + GS +NFW P +IRD G+K V+++ + S IP +GA Sbjct: 253 L------PILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGATA 306 Query: 314 LILIARRSDRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIF 373 ++ I R +++ + + + LAL TL P + ++L +++A ++ +F Sbjct: 307 MVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIALF 366 Query: 374 WSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNMDIALYILAGLLLLSG 433 W P T A GIA I+SVG G V P+L+G + T G+ L +L G L L+G Sbjct: 367 WGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGGALFLAG 426 Query: 434 LAMWSLIPRKAATAAT 449 + + +L RK AA Sbjct: 427 VLI-ALGGRKRGPAAV 441 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.141 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,142,952 Number of extensions: 40076 Number of successful extensions: 261 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 233 Number of HSP's successfully gapped: 20 Length of query: 450 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 362 Effective length of database: 1,203,190 Effective search space: 435554780 Effective search space used: 435554780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)