BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0056.p (501 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 949 0.0 gi|300314020|ref|YP_003778112.1| major facilitator superfamily p... 314 1e-86 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 139 5e-34 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 132 7e-32 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 121 1e-28 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 949 bits (2453), Expect = 0.0, Method: Compositional matrix adjust. Identities = 490/493 (99%), Positives = 491/493 (99%) Query: 9 LSRFLPIPAMELFSDHPNDEGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQ 68 +SRFLPIPAMELFSDHPNDEGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQ Sbjct: 1 MSRFLPIPAMELFSDHPNDEGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQ 60 Query: 69 TSAAVSIWVSNAYLLATAMTLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLL 128 TSAAVSIWVSNAYLLATAMTLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLL Sbjct: 61 TSAAVSIWVSNAYLLATAMTLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLL 120 Query: 129 ILMRILQGLGGAAMLSIAPAIHRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLATL 188 ILMRILQGLGGAAMLSIAPAIHRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLA L Sbjct: 121 ILMRILQGLGGAAMLSIAPAIHRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLAML 180 Query: 189 GWEWIFAINVPLGVLAVWLAWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARF 248 GWEWIFAINVPLGVLAVWLAWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARF Sbjct: 181 GWEWIFAINVPLGVLAVWLAWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARF 240 Query: 249 AEPGQGSEAAFTAAAYAVTALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASF 308 AEPGQGS AAFTAAAYAVTALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASF Sbjct: 241 AEPGQGSGAAFTAAAYAVTALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASF 300 Query: 309 VGQGIAFVALPFLFQNAYGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGL 368 VGQGIAFVALPFLFQNAYGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGL Sbjct: 301 VGQGIAFVALPFLFQNAYGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGL 360 Query: 369 ALFALGLVLLALLPDQAQPWDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVL 428 ALFALGLVLLALLPDQAQPWDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVL Sbjct: 361 ALFALGLVLLALLPDQAQPWDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVL 420 Query: 429 AIMRTFGQCLGAALLGVILAVRTAAAVAQGELQMNPVQDGEAIRIALWLAAAATALATAV 488 AIMRTFGQCLGAALLGVILAVRTAAAVAQGELQMNPVQDGEAIRIALWLAAAATALATAV Sbjct: 421 AIMRTFGQCLGAALLGVILAVRTAAAVAQGELQMNPVQDGEAIRIALWLAAAATALATAV 480 Query: 489 SASRLRRKAESPA 501 SASRLRRKAESPA Sbjct: 481 SASRLRRKAESPA 493 >gi|300314020|ref|YP_003778112.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 475 Score = 314 bits (804), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 188/416 (45%), Positives = 262/416 (62%), Gaps = 8/416 (1%) Query: 28 EGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWVSNAYLLATAM 87 EGLP +QR RA+LA+ A +A D I N ALP ++ LQT+ A S+W+ AY LA + Sbjct: 22 EGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALDLQTTPAASVWIVTAYQLAMMV 81 Query: 88 TLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGLGGAAMLSIAP 147 L AAL IG+R + GL +FT ASL CA++ S+ L++ R LQGLGGA ++S+ Sbjct: 82 ALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLPTLVMARFLQGLGGAGIMSVNT 141 Query: 148 AIHRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLATLGWEWIFAINVPLGVLAVWL 207 A+ R I+P R LGR +GLN ++VA + VGP + +LA W W+FA+NVPLGVLAV L Sbjct: 142 ALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILAIAPWPWLFAVNVPLGVLAVVL 201 Query: 208 AWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARFAEPGQGSEAAFTAAAYAVT 267 A ++P T A+ AFDL AL +A A G LI+A A A Q S AA Sbjct: 202 AVPSLPHTSL-ARHAFDLRSALLNAAAFGLLILAIGEGAHQA---QASVVGLELAA---- 253 Query: 268 ALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASFVGQGIAFVALPFLFQNAYG 327 A+L GL ++ + P+LP+D+F +P FSL+A+T++ SF QG+AFV+LPF F + G Sbjct: 254 AILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLAFVSLPFFFHHDLG 313 Query: 328 YTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFALGLVLLALLPDQAQP 387 + E L TPWP+ + ++AP AGR++DR+ +L +GLAL GL+ + +P Sbjct: 314 RSQVEIGLLMTPWPVAVAIMAPIAGRMSDRYPVGMLGGVGLALLCAGLLSMTFMPASPSV 373 Query: 388 WDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFGQCLGAALL 443 W+I WR L+CGMGFGFFQSPN + ++ + RSG ASG++A R GQ +GAAL+ Sbjct: 374 WEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLLGQSVGAALV 429 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 139 bits (350), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 139/454 (30%), Positives = 215/454 (47%), Gaps = 29/454 (6%) Query: 18 MELFSD--HPNDEGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSI 75 M L +D H + + LP ++ A L + + D TI+ ALP I L+ + Sbjct: 1 MALHTDSAHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELK-GFELYA 59 Query: 76 WVSNAYLLATAMTLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQ 135 WV+ AYLL + +T+ F L G + A + VF AS C ++ S+ L+L R LQ Sbjct: 60 WVATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQ 119 Query: 136 GLGGAAMLSIAPAIHRTIFPN-RLLGRILGLNAVLVAASTAVGPVLGGTLLATLGWEWIF 194 G+GG ++ A +FP+ R+ R + +V +TAVGP LGG L LGW W+F Sbjct: 120 GIGGGMLVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVF 179 Query: 195 AINVPLGVLAVWLAWNAVPDTRKPAQAA---FDLAGALWSALAMGALIMAADTC-ARFAE 250 N+P+GV++++ W VP R A D GAL +L++G+L + + R Sbjct: 180 YCNLPIGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVT 239 Query: 251 PGQGSEAAFTAAAYAVTALLAGLAFVRGQRRAADPLLPLDIFANPRF-SLAALTSLASFV 309 G A + AA+ A + ++RAA P+LP ++ + SL L+ LA F Sbjct: 240 SGMLVLLAVSVAAF--------YALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFA 291 Query: 310 GQGIAFVALPFLFQNAYGYTAFESAALFTPWPLGIVLVAPHAGRLADR-HSPALLSSLGL 368 + A P LFQ +G + E+ L TP I + + GR+ R +P L+ +LG Sbjct: 292 MFSMLMYA-PLLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGF 350 Query: 369 ALFA---LGLVLLALLPDQAQPWDIGWRALVCGMGFGFFQSPNNREMLGN--VSRERSGN 423 AL A LG+VL D+ ++ G GFG N + +RE G Sbjct: 351 ALIAASCLGIVLC----DRVTGKGFLLAVMLAG-GFGLGLVLPNLTVFAQQAAAREHLGI 405 Query: 424 ASGVLAIMRTFGQCLGAALLGVILAVRTAAAVAQ 457 A+ +L +R G LG A+ G +++ A V + Sbjct: 406 ATALLQSLRMIGGMLGTAITGTLVSQMYGAGVEK 439 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 132 bits (332), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 140/428 (32%), Positives = 197/428 (46%), Gaps = 37/428 (8%) Query: 37 RAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWVSNAYLLATAMTLAAFAALS 96 R +L M V D ++ + LP +++ L S + WV ++Y+L A L A + Sbjct: 36 RILLTGSFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATA 95 Query: 97 SRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGLGGAAMLS-----IAPAIHR 151 R G R G+A+F L SL CAL+ S L R LQG+G A +L+ I A H Sbjct: 96 DRYGRRRVLLGGIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHD 155 Query: 152 TIFPNR---LLGRILGLNAVLVAASTAVGPVLGGTLLATLGWEWIFAINVPLGVLAVWLA 208 R L G I+GL VL P++GG + LGW W FA+N+P +L Sbjct: 156 EDQRERAWALWGGIMGLTMVLA-------PLIGGVVNTLLGWRWAFAVNLPACLLLAVAV 208 Query: 209 WNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARFAEPGQGSEAAFTA---AAYA 265 W VP++R P D G L S+ A+ L A T G E +T+ A Sbjct: 209 WRHVPESRNPLPRPLDPLGILSSSSAVFLLTWALIT---------GPEHGWTSVACVARG 259 Query: 266 VTALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASFVGQGIAFVALPFLFQNA 325 + L + F+ QRRAA P+L L +F + F A A + LP QNA Sbjct: 260 LGGALLLVLFIAWQRRAAHPMLELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNA 319 Query: 326 YGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFALG--LVLLALLPD 383 G +A+++ P+ L ++L+ RLA S + +LGL + ALG L++ A L Sbjct: 320 RGLSAWQTGLAMLPFALAMLLLPQVGSRLARWLSSPQMLALGLGVTALGNLLMIQAALSG 379 Query: 384 QAQPWDIGWRAL---VCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFGQCLGA 440 A W AL V G G G + ++GNV RER+G ASG+ R G G Sbjct: 380 AA-----AWTALGMAVLGSGGGLLNGQTQKAIMGNVPRERAGMASGISTTARFTGVLAGF 434 Query: 441 ALLGVILA 448 A LG +LA Sbjct: 435 AGLGAVLA 442 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 121 bits (304), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 108/409 (26%), Positives = 187/409 (45%), Gaps = 14/409 (3%) Query: 30 LPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWVSNAYLLATAMTL 89 L G Q A + + A +AV D TI NV++ I+ AL S + +V +Y +A A+T+ Sbjct: 26 LSGGQLVGAGILLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITV 85 Query: 90 AAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGLGGAAMLSIAPAI 149 L++R G F + +F L S C ++ S+ +LI R+LQGL G ++ ++ + Sbjct: 86 PLTGWLANRFGTVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTL 145 Query: 150 HRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLATLGWEWIFAINVPLGVLAVWLAW 209 IFP +GL A+ + GP+LGG L W +IF INVP+ ++ ++ W Sbjct: 146 LLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGW 205 Query: 210 NAVPDTR-KPAQAAFDLAGALWSALAMGALIMAADTCARFAEPGQGSEAAFTAAA----Y 264 + K ++ D G + + + AL + D +G E + A+ Sbjct: 206 RMLKRYETKMSKLPIDRVGLVLMVVWVAALQLMLD---------EGKEKDWFASTEIVLL 256 Query: 265 AVTALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASFVGQGIAFVALPFLFQN 324 AVTA++ +AF+ + +P++ L +F + F+ + T +F + V P Q Sbjct: 257 AVTAVVFFIAFLIWELTERNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQG 316 Query: 325 AYGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFALGLVLLALLPDQ 384 GYTA S + + V+ AP +++ L +G+ L A Sbjct: 317 YMGYTATWSGCISALTGILAVMTAPMVAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTD 376 Query: 385 AQPWDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRT 433 + IGW + G+G F P L +V+ E + +A+G+++ RT Sbjct: 377 MSFFQIGWPLFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLMSFCRT 425 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,330,031 Number of extensions: 50333 Number of successful extensions: 405 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 379 Number of HSP's successfully gapped: 17 Length of query: 501 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 412 Effective length of database: 1,198,455 Effective search space: 493763460 Effective search space used: 493763460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)