BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0050.p (620 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 1258 0.0 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 240 2e-64 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 226 3e-60 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 188 1e-48 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 173 4e-44 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 1258 bits (3256), Expect = 0.0, Method: Compositional matrix adjust. Identities = 613/620 (98%), Positives = 613/620 (98%) Query: 1 MPTYRSYTTTQGRNMAGARALWRATGMKDGDFTKPIIAVVNSFTQFVPGHVHLKDLGQLV 60 MPTYRSYTTTQGRNMAGARALWRATGMKDGDFTKPIIAVVNSFTQFVPGHVHLKDLGQLV Sbjct: 1 MPTYRSYTTTQGRNMAGARALWRATGMKDGDFTKPIIAVVNSFTQFVPGHVHLKDLGQLV 60 Query: 61 AREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDLIADSVEYMVNAHCADAMVCIS 120 AREIEAAGGVAKEFNTIAVDD G MLYSLPSRDLIADSVEYMVNAHCADAMVCIS Sbjct: 61 AREIEAAGGVAKEFNTIAVDDSGTHGDARMLYSLPSRDLIADSVEYMVNAHCADAMVCIS 120 Query: 121 NCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVIKTVNTDKKVIKLDLVDAMIQAGDKNI 180 NCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVIKTVNTDKKVIKLDLVDAMIQAGDKNI Sbjct: 121 NCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVIKTVNTDKKVIKLDLVDAMIQAGDKNI 180 Query: 181 SDADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAG 240 SDADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAG Sbjct: 181 SDADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAG 240 Query: 241 RLIVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEF 300 RLIVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEF Sbjct: 241 RLIVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEF 300 Query: 301 TMADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKT 360 TMADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKT Sbjct: 301 TMADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKT 360 Query: 361 LGEAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDREHAY 420 LGEAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDREHAY Sbjct: 361 LGEAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDREHAY 420 Query: 421 SQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGDKVKAGDVV 480 SQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGDKVKAGDVV Sbjct: 421 SQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGDKVKAGDVV 480 Query: 481 IVRYEGPKGGPGMQEMLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGG 540 IVRYEGPKGGPGMQEMLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGG Sbjct: 481 IVRYEGPKGGPGMQEMLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGG 540 Query: 541 AIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRTAMEARGDAAWQPGNRQRVVSQALQAY 600 AIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRTAMEARGDAAWQPGNRQRVVSQALQAY Sbjct: 541 AIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRTAMEARGDAAWQPGNRQRVVSQALQAY 600 Query: 601 AALTTSADRGAVRDLSQLKR 620 AALTTSADRGAVRDLSQLKR Sbjct: 601 AALTTSADRGAVRDLSQLKR 620 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 240 bits (613), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 182/622 (29%), Positives = 313/622 (50%), Gaps = 81/622 (13%) Query: 5 RSYTTTQGRNMAGARALWRATGMKDGDFT-KPIIAVVNSFTQFVPGHVHLKDLGQLVARE 63 +++ Q R+ R+ + G+ F +P+I + N++++ P + H + L + V Sbjct: 13 QAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSHFRALAEQVKIG 72 Query: 64 IEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCD 123 I AGG EF +++ + + + MLY R+L + VE + A+ D +V + CD Sbjct: 73 IWEAGGFPLEFPVMSLGETL-LRPTAMLY----RNLASMDVEESIRANPLDGVVLLMGCD 127 Query: 124 KITPGMLMAAMRLNIPVVFVSGGPMEAGKVI-KTVNTDKKVIKLDLVDAMIQAGDKNISD 182 K TP +LM A +++P + VSGGPM +GK + + + V ++ ++AG ++ Sbjct: 128 KTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMS---EDVRAG--KMTQ 182 Query: 183 ADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAGRL 242 + E E + G C M TA++M + E LG+ LP N I A A R L AGR Sbjct: 183 EEFFEAESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRR 242 Query: 243 IVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEFTM 302 IV++ E+D ++ I T++ +ENA+ ++ ++GGSTN V+HLLA A ++ + Sbjct: 243 IVQMV----EED---LVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDL 295 Query: 303 ADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKTLG 362 D D I +++PCL + P + K+ +ED + AGG+ +++ +L ++D + T + KTL Sbjct: 296 KDWDDIGQQLPCLLDLQP-SGKFLMEDFYYAGGLPAVIRQL--ESVIDKTALTANGKTLW 352 Query: 363 EAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDREHAYSQ 422 E + AP +++E IR + + + Sbjct: 353 ENCQD-----------------APN-------WNEE-------------VIRSFDKPFKE 375 Query: 423 DGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGDKVKAGD--VV 480 G+A+L GN+A G ++K + ++LK G A VFE+ D + I + + + V+ Sbjct: 376 AAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVL 435 Query: 481 IVRYEGPKGGPGMQE---MLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAA 537 +++ GPKG PGM E M P ++ KG+ + +D R SG + G V+ H SPEAA Sbjct: 436 VLKNCGPKGYPGMAEAGNMPLPPKILR-KGITDMVRV-SDARMSGTAYGTVVLHVSPEAA 493 Query: 538 EGGAIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRTAMEARGDAAWQ----PGNRQRVV 593 GG + +V+ GD I++D+ R ++L +S ++LA+RR A WQ P QR Sbjct: 494 AGGPLAVVQNGDFIELDVEARKLHLDVSDEELARRR--------AQWQKPELPPQMQR-- 543 Query: 594 SQALQAYAALTTSADRGAVRDL 615 ++ Y A++GA D Sbjct: 544 -GWVKLYVDHVQQANQGADLDF 564 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 226 bits (577), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 186/611 (30%), Positives = 300/611 (49%), Gaps = 72/611 (11%) Query: 3 TYRS--YTTTQGRNMAGARALWRATGMKDGDFT-KPIIAVVNSFTQFVPGHVHLKDLGQL 59 T RS + T +N R+ + G+ D +F KP+I + N++++ P + H + + + Sbjct: 11 TLRSAGWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRKIAEH 70 Query: 60 VAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDLIADSVEYMVNAHCADAMVCI 119 V R I AGG EF + + + ML +R+L + VE + + DA+V + Sbjct: 71 VRRGIIEAGGFPVEFPVFSNGES-NLRPTAML----TRNLASMDVEESIRGNPIDAVVLL 125 Query: 120 SNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVI-KTVNTDKKVIKLDLVDAMIQAGDK 178 + CDK TP +LM A ++P + V+GGPM GK + + + V +L ++AG+ Sbjct: 126 TGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQL---SEQVKAGEI 182 Query: 179 NISDADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLR 238 I D AE S + G+C+ M TA++M C+ E LG+SLP N I A A R L Sbjct: 183 TIHDFMAAEAGMSR--SAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHL 240 Query: 239 AGRLIVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKV 298 +G IV++ +E S I T++ +ENA+ + ++GGSTN V+HL A A V Sbjct: 241 SGMRIVDMV---WEGLTLS----KILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGV 293 Query: 299 EFTMADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRP-TVH 357 + + D RI R P + + P + +Y +E+ + AGG+ ++L L A LL TV+ Sbjct: 294 DLELEDWTRIGRGTPTIVDLQP-SGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVN 352 Query: 358 SKTLGEAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDRE 417 +T+ + ++ D + +D V L + Sbjct: 353 GQTMWDNVK--DAPIYNDEVVRPLAK---------------------------------- 376 Query: 418 HAYSQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGD--KVK 475 +DGG+ +L GN+A +G ++K + ++K G A VFE + E I V Sbjct: 377 -PLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVD 435 Query: 476 AGDVVIVRYEGPKGGPGMQE---MLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHA 532 A V++++ GPKG PGM E M P + + G+ K +D R SG + G VI H Sbjct: 436 ASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLAT-GV-KDMVRISDARMSGTAYGTVILHV 493 Query: 533 SPEAAEGGAIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRTAMEARGDAAWQP----GN 588 +PEAA GG +G+V++GD I++D + L IS +++ KRR A+ A +P G Sbjct: 494 APEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEM-KRRLEARAKVLAERKPEMVGGY 552 Query: 589 RQRVVSQALQA 599 + V + LQA Sbjct: 553 QSLYVDRVLQA 563 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 188 bits (477), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 163/558 (29%), Positives = 266/558 (47%), Gaps = 75/558 (13%) Query: 34 KPIIAVVNSFTQFVP-GHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLY 92 +PII + S + P +HL +L + V I AGG+ EF + + + Sbjct: 47 RPIIGIAQSGSDISPCNRIHL-ELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAI-- 103 Query: 93 SLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGK 152 R+L + +++ + DA+V + CDK TP +MAA ++IP + +SGGPM G Sbjct: 104 ---DRNLAYLGLVEILHGYPIDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGW 160 Query: 153 VI-KTVNTDKKVIKLDLVDAMIQAGDKNISDADVAEVERSACPTCGSCSGMFTANSMNCL 211 + + V + + K ++ AG +I + E+ ++ P+ G C+ M TA++MN + Sbjct: 161 MDGELVGSGSAIWK---GRKLLSAG--SIDNEKFLEIAAASAPSSGHCNTMGTASTMNAM 215 Query: 212 TEVLGLSLPGNGTIVATHADRKELFLRAGRLIVELAKRHYEQDDYSILPRNICTKETWEN 271 E LG+SL G I A + +R ++ GR IV +A YE + P I T++ + + Sbjct: 216 AEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMA---YED----LRPSAILTRDAFLD 268 Query: 272 AMTLDVSMGGSTNTVLHLLAAAHEAKVEFTMADIDRISRRVPCLCKVAPMTNKYHIEDVH 331 A+ ++ ++GGSTN H++A A A VE D + VP L + P KY E H Sbjct: 269 AIVVNAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYGYDVPLLLNMQP-AGKYLGERFH 327 Query: 332 RAGGIISILGELARAGLLDTSRPTVHSKTLGEAIEKFDIRVSSDPAVHKLFRAAPGGVPT 391 RAGG+ +I+ EL +AG L R T KT+ E ++ R S+D + F AAP Sbjct: 328 RAGGVPAIMWELQQAGKLRAERITATGKTMAENLQG---RASNDREMIYPF-AAP----- 378 Query: 392 QVAFSQEERFEANDLDRANGCIRDREHAYSQDGGLAVLYGNIAEKGCIVKTAGVDESI-- 449 +R+R G VL GN+ + I+KT+ + E+ Sbjct: 379 ---------------------LRER-------AGFLVLKGNLFDF-AIMKTSVISETFRE 409 Query: 450 --LKFSGTARVFESQDAAVEGI------LGD---KVKAGDVVIVRYEGPKGGPGMQEM-- 496 L G +FE + +G + D K+ ++ +R GP G PG E+ Sbjct: 410 RYLSTPGQENIFECRAVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVN 469 Query: 497 LYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDIDIP 556 + P + +G+ L DGR SG S I +ASPE+A GG + + +GD + ID+ Sbjct: 470 MQPPDALIKRGVSTLPTL-GDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLN 528 Query: 557 NRTINLRISSDDLAKRRT 574 N+ +S ++LA+R++ Sbjct: 529 TGECNMLVSEEELARRKS 546 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 173 bits (438), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 157/545 (28%), Positives = 251/545 (46%), Gaps = 45/545 (8%) Query: 35 PIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAK-EFNTIAVDDGIAMGHDGMLYS 93 P +A+V+++ + H + Q++ AG VA+ + A+ DG+ G GM S Sbjct: 68 PSLAIVSAYNDMLSAHQPFERFPQIIKDAAREAGAVAQFAGGSPAMCDGVTQGQAGMELS 127 Query: 94 LPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRL-NIPVVFVSGGPMEAGK 152 L SRD IA + ++ + DA + + CDKI PG+L+ A+ ++P VFV GPM +G Sbjct: 128 LFSRDAIAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMTSG- 186 Query: 153 VIKTVNTDKKVIKLDLVDAMIQAGDKNISDADVAEVERSACPTCGSCSGMFTANSMNCLT 212 N+DK I+ + AG I A++ E E + G+C+ TANS L Sbjct: 187 ---MSNSDKVKIR-----QLYTAG--KIGRAELLEAESKSYHGAGTCTFYGTANSNQMLM 236 Query: 213 EVLGLSLPGNGTIVATHADRKELFLRAGRLIVELAKRHYEQDDYSILPRNICTKETWENA 272 E +GL LPG I R L A + ++ + Y+ + R I K + NA Sbjct: 237 EAMGLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGTQ---YTPVGRMIDEK-SIVNA 292 Query: 273 MTLDVSMGGSTNTVLHLLAAAHEAKVEFTMADIDRISRRVPCLCKVAPMTNKYHIEDVHR 332 + + GGSTN LHL+A A A + D D++S VP + K+ P + H Sbjct: 293 IVALHATGGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYP-NGTADVNHFHA 351 Query: 333 AGGIISILGELARAGLLDTSRPTVHSKTLGEAIEKFDIRVSSDPAVHKLFRAAPG--GVP 390 AGG +L EL AGL+ T+ LG+ + + +P + AA G G P Sbjct: 352 AGGTGFVLRELIDAGLMHDDVTTI----LGQGLRQH----CKEPML-----AAEGENGRP 398 Query: 391 TQVAFSQEERFEANDLDRANGCIRDREHAYSQDGGLAVLYGNIAEKGCIVKTAGVDESIL 450 V + ++ G + ++ DGGL +L GN+ ++K + V Sbjct: 399 GIVTWHDAPAQSGDE-----GVLGTVAKPFAADGGLKLLQGNLGR--AVIKISAVKPEHR 451 Query: 451 KFSGTARVFESQDAAVEGILGDKVKAGDVVIVRYEGPK--GGPGMQEMLYPTSYIKSKGL 508 A VF SQ+A + ++ V ++ ++GP+ G P + + ++ K Sbjct: 452 VVEAPAVVFHSQEAFMAAFKAGQLDRDFVAVITFQGPRANGMPELHALTPALGILQDK-- 509 Query: 509 GKACALFTDGRFSGGSSGLVIG-HASPEAAEGGAIGLVEEGDMIDIDIPNRTINLRISSD 567 G+ AL TDGR SG S + H +PE GG +G V +GD+I +D T+ ++ + Sbjct: 510 GRHVALVTDGRMSGASGKVPAAIHVTPEVLGGGPLGRVRDGDIIRLDAEAGTLEAKVDAT 569 Query: 568 DLAKR 572 + A R Sbjct: 570 EWAAR 574 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,990,359 Number of extensions: 85686 Number of successful extensions: 311 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 288 Number of HSP's successfully gapped: 8 Length of query: 620 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 529 Effective length of database: 1,188,985 Effective search space: 628973065 Effective search space used: 628973065 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)