BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0049.p (488 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309866|ref|YP_003773958.1| major facilitator superfamily p... 896 0.0 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 424 e-120 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 154 1e-38 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 115 9e-27 gi|300313658|ref|YP_003777750.1| transport transmembrane protein... 103 4e-23 >gi|300309866|ref|YP_003773958.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 484 Score = 896 bits (2315), Expect = 0.0, Method: Compositional matrix adjust. Identities = 477/490 (97%), Positives = 477/490 (97%), Gaps = 8/490 (1%) Query: 1 MHHVDAADPVGRGSSAASVAPAASAASAVSAASNAPAAAQIGQKPTRYRWVVMGLIFLIW 60 MHHVDAADPVGRGSSAASVAPAASAAS AA AQIGQKPTRYRWVVMGLIFLIW Sbjct: 1 MHHVDAADPVGRGSSAASVAPAASAASNAPAA------AQIGQKPTRYRWVVMGLIFLIW 54 Query: 61 AIACADRANFGIALPYMKKEYGITNTEAGLIVSLFSFAYGLVQIPVGLMYKRLSEKTTGI 120 AIACADRANFGIALPYMKKEYGITNTEAGLIVSLFSFAYGLVQIPVGLMYKRLSEKTTGI Sbjct: 55 AIACADRANFGIALPYMKKEYGITNTEAGLIVSLFSFAYGLVQIPVGLMYKRLSEKTTGI 114 Query: 121 LFSVFMLLTSLFTGLMGTTSSVLLLQLYRVGLGLSEGPLGIGCTNVINRWFPPEEKGTAT 180 LFSVFMLLTSLFTGLMGTTSSVLLLQLYRVGLGLSEGPLGIGCTNVINRWFPPEEKGTAT Sbjct: 115 LFSVFMLLTSLFTGLMGTTSSVLLLQLYRVGLGLSEGPLGIGCTNVINRWFPPEEKGTAT 174 Query: 181 GLWIAASKLGPLIVPTVCIIVIQLWGWREIFYVFAVPGIFLAILWFFLVTNSPSENRFCN 240 GLWIAASKLGPLIVPTVCIIVIQLWGWREIFYVFAVPGIFLAILWFFLVTNSPSENRFCN Sbjct: 175 GLWIAASKLGPLIVPTVCIIVIQLWGWREIFYVFAVPGIFLAILWFFLVTNSPSENRFCN 234 Query: 241 EAERRYITQDVAAGP--ATASSRAHVGAQVTPMPWLDGINRTRRVPRLETLRQVFTSWNV 298 EAERRYITQDVAAGP ATASSRAHVGAQVTPMPWLDGINRTRRVPRLETLRQVFTSWNV Sbjct: 235 EAERRYITQDVAAGPATATASSRAHVGAQVTPMPWLDGINRTRRVPRLETLRQVFTSWNV 294 Query: 299 IGVAIGYGCMIGISNIFMSWIPTYLVTVKGFASVKMGFLASAPFIGAVAGNMLGGVISDR 358 IGVAIGYGCMIGISNIFMSWIPTYLVTVKGFASVKMGFLASAPFIGAVAGNMLGGVISDR Sbjct: 295 IGVAIGYGCMIGISNIFMSWIPTYLVTVKGFASVKMGFLASAPFIGAVAGNMLGGVISDR 354 Query: 359 LLGGRRKPMMLLGALGTALMTLLLVEAPDSVLYLGAMLMFSGLMLGIGFAGYSAYPMGLA 418 LLGGRRKPMMLLGALGTALMTLLLVEAPDSVLYLGAMLM SGLMLGIGFAGYSAYPMGLA Sbjct: 355 LLGGRRKPMMLLGALGTALMTLLLVEAPDSVLYLGAMLMLSGLMLGIGFAGYSAYPMGLA 414 Query: 419 TKATYPAAFGIVNSLGQIGGACAPLAVGMLLDRYDWSSVFLYMVGTSLLCLVLLLSVVEP 478 TKATYPAAFGIVNSLGQIGGACAPLAVGMLLDRYDWSSVFLYMVGTSLLCL LLLSVVEP Sbjct: 415 TKATYPAAFGIVNSLGQIGGACAPLAVGMLLDRYDWSSVFLYMVGTSLLCLALLLSVVEP 474 Query: 479 ITVRKAGEEA 488 ITVRKAGEEA Sbjct: 475 ITVRKAGEEA 484 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 424 bits (1090), Expect = e-120, Method: Compositional matrix adjust. Identities = 213/445 (47%), Positives = 307/445 (68%), Gaps = 11/445 (2%) Query: 37 AAAQIGQKPTRYRWVVMGLIFLIWAIACADRANFGIALPYMKKEYGITNTEAGLIVSLFS 96 AA Q +K T +RWVV LIF+++ +A ADRAN G+ALP+++KE+ ++NTEAG + S+F Sbjct: 4 AAIQHTKKKTHFRWVVGALIFIVYTVAAADRANLGVALPFLRKEFVMSNTEAGALASIFL 63 Query: 97 FAYGLVQIPVGLMYKRLSEKTTGI--LFSVFMLLTSLFTGLMGTTSSVLLLQLYRVGLGL 154 FAY L Q+P GL Y R GI +FS+ ML TS+FTGL+GT SS+L L+++R GLGL Sbjct: 64 FAYSLAQLPSGLAYTRF-----GIPRVFSLAMLATSVFTGLIGTASSLLALKIFRFGLGL 118 Query: 155 SEGPLGIGCTNVINRWFPPEEKGTATGLWIAASKLGPLIVPTVCIIVIQLWGWREIFYVF 214 +EGPL IG T+ IN WFP EKGT TG+++AA+K GP++VP VC +++ WGWR IFY F Sbjct: 119 AEGPLPIGITSTINNWFPAREKGTMTGIFLAAAKFGPVLVPPVCAVIMYYWGWRHIFYFF 178 Query: 215 AVPGIFLAILWFFLVTNSPSENRFCNEAERRYITQDVAAGPATASSRAHVGAQVTPMPWL 274 AVPG+ L+++W +V N PSE+ + E +I A A+ + A L Sbjct: 179 AVPGVILSVVWLMVVKNRPSESPHVSAEELEHINDSQGA----AAGQDADPAVTRDFALL 234 Query: 275 DGINRTRRVPRLETLRQVFTSWNVIGVAIGYGCMIGISNIFMSWIPTYLVTVKGFASVKM 334 D I R +V +ET + VF SWN++G A+GY M I N+ ++WIPTYL++VKG++ M Sbjct: 235 DRIIRRSKVATIETTKGVFCSWNIMGAALGYFFMTAIVNVLLAWIPTYLISVKGYSIANM 294 Query: 335 GFLASAPFIGAVAGNMLGGVISDRLLGGRRKPMMLLGALGTALMTLLLVEAPDSVLYLGA 394 G +A+AP++GAV GN+LGG ++DR L RRKP M++ A T +M ++L+ +P L GA Sbjct: 295 GMVAAAPWVGAVIGNLLGGAMTDRWLEKRRKPAMMMSAFFTTIMMVILINSPADPLLYGA 354 Query: 395 MLMFSGLMLGIGFAGYSAYPMGLATKATYPAAFGIVNSLGQIGGACAPLAVGMLLDRYDW 454 +L+ +G++L IG++ Y YPMG+ +K YP A +VN+LGQ+GGACAPLA G LLD Y W Sbjct: 355 LLLLTGILLNIGYSTYMVYPMGMTSKKVYPMACAMVNTLGQLGGACAPLAAGFLLDSYSW 414 Query: 455 SSVFLYMVGTSLLCLVLLLSVVEPI 479 + V+++M S+LC + +L++ EP+ Sbjct: 415 NHVWIFMAVCSILCFITILTIREPV 439 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 154 bits (390), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 135/460 (29%), Positives = 218/460 (47%), Gaps = 59/460 (12%) Query: 44 KPTRYRWVVMGLIFLIWAIACADRANFGIALPYMKKEYGITNTEAGLIVSLFSFAYGLVQ 103 KPT R++++ L+F++ + ADRA IA MK E + G I S FS+AY L Q Sbjct: 7 KPTHVRYLILFLLFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQ 66 Query: 104 IPVGLMYKRLSEKTTGI----LFSVFMLLTSLFTGLMGTTSSVLLLQLYRVGLGLSEGPL 159 +P G + R K + L+SVF L + T M T ++V L L R +GL+E P Sbjct: 67 LPGGWLLDRFGSKKIYMWSIALWSVFTFLQAGVT-WMSTAAAVASLFLLRFMVGLAEAPS 125 Query: 160 GIGCTNVINRWFPPEEKGTATGLWIAASKLGPLIVPTVCIIVIQLWGWREIFYVFAVPGI 219 ++ WFP +E+GTA+ ++ +A ++ + ++ + W +F V V GI Sbjct: 126 FPANGRIVAAWFPTKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGI 185 Query: 220 FLAILWFFLVTNSPSENRFCNEAERRYITQDVAAGPATASSRAHVGAQVTPMPWLDGINR 279 LA++W ++ SP ++ N+AE +I G + M NR Sbjct: 186 ALALIWGRII-YSPKDHPRINQAELAHIES---------------GGGLVDMD-----NR 224 Query: 280 TRRVPRLE---TLRQVFTSWNVIGVAIGYGCMIGISNIFMSWIPTYLVTVKGFASVKMGF 336 P + +RQ+ ++ ++GV IG C+ I+ F++W P YLV +G + +K GF Sbjct: 225 KVAAPSTKGRGYIRQLLSNRMLLGVYIGQYCINVITYFFLTWFPVYLVQERGMSILKAGF 284 Query: 337 LASAPFIGAVAGNMLGGVISDRLLG-------GRRKPM---MLLGALGTALMTLLLVEAP 386 +AS P + G ++GG+ISDRL+ R+ P+ MLL MT++L Sbjct: 285 VASIPAVCGFIGGVVGGLISDRLIKRGLSVTWARKIPIVGGMLLS------MTMILCN-- 336 Query: 387 DSVLYLGAMLMFSGLMLGIGFAGYSAYPMGLATKA-TYPAAF-----GIVNSLGQIGGAC 440 Y+ A M G+M + F G +G A A T P G+ N G + G Sbjct: 337 ----YVEAQWMVVGIM-ALAFFGKGVGALGWAVVADTAPKQIVGLSGGLFNMFGNVAGIT 391 Query: 441 APLAVGMLLDRYDWSSVFLYMVG-TSLLCLVLLLSVVEPI 479 P+ +G ++ S L VG +L+ ++ L +V+ I Sbjct: 392 TPIVIGYIVKETGSFSGALVFVGINALVTVISYLVIVKEI 431 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 115 bits (288), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 102/424 (24%), Positives = 189/424 (44%), Gaps = 45/424 (10%) Query: 40 QIGQKPTRYRWVVMGLIFLIWA--IACADRANFGIALPYMKKEYGITNTEAGLIVSLFSF 97 Q + P R V+ L FL+ A + DR+ IA + +E ++ ++ GL++S FS Sbjct: 4 QKEKSPRLKRIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSL 63 Query: 98 AYGLVQIPVGLMYKRLSEKTTGILFSVFMLLTSLFTGLMGTTSSVLLLQLYRVGLGLSEG 157 AY Q+P+G M + + ++ M + S+ G +++ + RV LGL E Sbjct: 64 AYAFSQLPIGAMLDKFGAR---VMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEA 120 Query: 158 PLGIGCTNVINRWFPPEEKGTATGLWIAASKLGPLIVPTVCIIVIQLWGWREIFYVFAVP 217 P V++ WF E+G TG+++A+S +GP + P + I++ +GWR +F + V Sbjct: 121 PQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVL 180 Query: 218 GIFLAILWFFLVTNSPSENRFCNEAERRYITQDVAAGPATASSRAHVGAQVTPMPWLDGI 277 GI +++ W+ N +E Y+ D P + A+++ W Sbjct: 181 GIAVSVGWYLFYRNRNEVQ--LTPSEVLYLADDHDNDP-------NANAKLSWSEW---- 227 Query: 278 NRTRRVPRLETLRQVFTSWNVIGVAIGYGCMIGISNIFMSWIPTYLVTVKGFASVKMGFL 337 + +FT G+ G+ +I + ++++W+P YL + + + G++ Sbjct: 228 ------------KSLFTYRTTWGMLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWV 275 Query: 338 ASAPFIGAVAGNMLGGVISDRLLG------GRRKPMMLLGALGTALMTLLLVEAPDS--- 388 P+I G + G I+D L+ RK + G +G T+ P + Sbjct: 276 VVIPYIFGTLGMLSSGYIADGLMKRGMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMA 335 Query: 389 VLYLGAMLMFSGLMLGIGFAGYS-AYPMGLATKATYPAAFGIVNSLGQIGGACAPLAVGM 447 ++Y+ + F + G +A S A P L FG G +GG+ AP+ G+ Sbjct: 336 IVYISLAMAFVNMASGGAWALVSVAIPRRLVASLGSMQNFG-----GYLGGSLAPVITGV 390 Query: 448 LLDR 451 ++D+ Sbjct: 391 VVDQ 394 >gi|300313658|ref|YP_003777750.1| transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 103 bits (256), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 98/417 (23%), Positives = 182/417 (43%), Gaps = 47/417 (11%) Query: 60 WAIACADRANFGIALPYMKKEYGITNTEAGLIVSLFSFAYGLVQIPVGLMYKRLSEKTTG 119 W ++ DRA A + E+ + ++ G+++S F Y L+Q+P G + R + Sbjct: 22 WCVSYIDRAAITFAATQIASEFHLKPSDLGILLSSFFLGYSLLQLPGGWLADRYGSRPV- 80 Query: 120 ILFSVFMLLTSLFTGLMGTTSSVLLLQLYRVGLGLSEGPLGIGCTNVINRWFPPEEKGTA 179 I+ S+ LL SLFTG+ G SV L + R G+ EG + F +E+ Sbjct: 81 IVISI--LLWSLFTGVTGMAWSVTSLVMIRFIFGVGEGAFPAASVKGVAENFSRDERPKM 138 Query: 180 TGLWIAASKLGPLIVPTVCIIVIQLWGWREIFYVFAVPGIFLAILWFFLVTNSPSENRFC 239 + L ++++ +G ++ P + +I +GWR +F+ + G+ A++++ FC Sbjct: 139 SSLLMSSNYIGSMLAPLLIAPLILHYGWRAVFHYIGIAGVVFALVYW-----------FC 187 Query: 240 NEAERRYITQDVAAGPATASSRAHVGAQVTPMPWLDGINRTRRVPRLETLRQVFTSWNVI 299 R + G + +A + P+ W Q+ W Sbjct: 188 VRPVRPGV-----GGAGAINKQAFMALMKMPLMW-----------------QIVAVW--F 223 Query: 300 GVAIGYGCMIGISNIFMSWIPTYLVTVKGFASVKMGFLASAPFIGAVAGNMLGGVISDRL 359 G++I I+ +W+PTYL+TV+G +G L P+I A +GG + R Sbjct: 224 GLSI-------INKGLDTWMPTYLMTVRGLNLKAVGLLLPLPYIMAGLSTAIGGWVMVRF 276 Query: 360 LGGRRKPMMLLGALGTALMTLLLVEAPDSVLYLGAMLMFSGLMLGIGFAGYSAYPMGLAT 419 GR K +++ +L TA+ + A SV + A + A A P + Sbjct: 277 FDGREKLLLIGSSLLTAVFVYCMYSAA-SVEGVIAWQCLAYFFKSFVLATCIALPTKMLQ 335 Query: 420 KATYPAAFGIVNSLGQIGGACAPLAVGMLLDRY-DWSSVFLYMVGTSLLCLVLLLSV 475 + G+VN GQ+ G +PL +G L+ + ++ F +++G + +++ L + Sbjct: 336 AQLVGSGVGMVNLGGQLAGFISPLVIGFLVSAFANYDYAFSFLIGAAFFSVLVSLFI 392 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.139 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,399,894 Number of extensions: 55086 Number of successful extensions: 424 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 398 Number of HSP's successfully gapped: 23 Length of query: 488 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 399 Effective length of database: 1,198,455 Effective search space: 478183545 Effective search space used: 478183545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)