BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0034.p (525 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309852|ref|YP_003773944.1| SAM-dependent methyltransferase... 1068 0.0 gi|300310113|ref|YP_003774205.1| SAM-dependent methyltransferase... 193 2e-50 >gi|300309852|ref|YP_003773944.1| SAM-dependent methyltransferase [Herbaspirillum seropedicae SmR1] Length = 525 Score = 1068 bits (2761), Expect = 0.0, Method: Compositional matrix adjust. Identities = 521/525 (99%), Positives = 523/525 (99%) Query: 1 LNNWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPTVQTACELGFGQGLSSNIHAA 60 +NNWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAP VQTACELGFGQGLSSNIHAA Sbjct: 1 MNNWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPAVQTACELGFGQGLSSNIHAA 60 Query: 61 ASTTRWWGNDFNPRQAAFAQELAASSGSGAQLEDSSFSRFCRRDDLPDFDFIALHGIWSW 120 ASTTRWWGNDFNPRQAAFAQELAA+SGSGAQLEDSSFSRFCRRDDLPDFDFIALHGIWSW Sbjct: 61 ASTTRWWGNDFNPRQAAFAQELAAASGSGAQLEDSSFSRFCRRDDLPDFDFIALHGIWSW 120 Query: 121 VSQENRDIIFDFIDRKLKPGGVLYISYNTQPGWSAFMPLRELLVQHTQQSGNEEQGLPTA 180 VSQENRDIIFDFIDRKLKPGGVLYISYNTQPGWSAFMPLRELLVQHTQQSGNEEQGLPTA Sbjct: 121 VSQENRDIIFDFIDRKLKPGGVLYISYNTQPGWSAFMPLRELLVQHTQQSGNEEQGLPTA 180 Query: 181 ARIDAALAFAARLFEADPVYAQANPFMRDRLKVLQQQSRSYLAHEYFNRDWHVESFADMH 240 ARIDAALAFAARLFEADPVYAQANPFMRDRLKVLQQQSRSYLAHEYFNRDWHVESFADMH Sbjct: 181 ARIDAALAFAARLFEADPVYAQANPFMRDRLKVLQQQSRSYLAHEYFNRDWHVESFADMH 240 Query: 241 RRFSGAGLEFACPAHYVDHLDMANLTPAQRQQLYEITDPVLQQSVRDFMVNQQFRRDYWV 300 RRFSGAGLEFACPAHYVDHLDMANLTPAQRQQLYEITDPVLQQSVRDFMVNQQFRRDYWV Sbjct: 241 RRFSGAGLEFACPAHYVDHLDMANLTPAQRQQLYEITDPVLQQSVRDFMVNQQFRRDYWV 300 Query: 301 RGAQALDDRQRVQALALQSVILLTEPDKVPLTIQNVASEITLNRLIYEPILQALSDYQPH 360 RGAQALDDRQRVQALALQSVILLTEPDKVPLTIQNVASEITLNRLIYEPILQALSDYQPH Sbjct: 301 RGAQALDDRQRVQALALQSVILLTEPDKVPLTIQNVASEITLNRLIYEPILQALSDYQPH 360 Query: 361 TLVDLAASLAHRNLNLAQVLDSVLMLIGTGHAAPLPAEPDAAARAQIERRTGDLNAWLLQ 420 TLVDLAASLAHRNLNLAQVLDSVLMLIGTGHAAPLPAEPDAA RAQIERRTGDLNAWLLQ Sbjct: 361 TLVDLAASLAHRNLNLAQVLDSVLMLIGTGHAAPLPAEPDAATRAQIERRTGDLNAWLLQ 420 Query: 421 RAALASPAGEQGEEGEITHLSSPVIGGGVRVDRVEQLFLLARQSCGDDVDAWPAQVWRYI 480 RAALASPAGEQGEEGEITHLSSPVIGGGVRVDRVEQLFLLARQSCGDDVDAWPAQVWRYI Sbjct: 421 RAALASPAGEQGEEGEITHLSSPVIGGGVRVDRVEQLFLLARQSCGDDVDAWPAQVWRYI 480 Query: 481 AAQGKRLLREGVRLEGEAENLAELATQARLFARTRLPLLKALGVV 525 AAQGKRLLREGVRLEGEAENLAELATQARLFARTRLPLLKALGVV Sbjct: 481 AAQGKRLLREGVRLEGEAENLAELATQARLFARTRLPLLKALGVV 525 >gi|300310113|ref|YP_003774205.1| SAM-dependent methyltransferase [Herbaspirillum seropedicae SmR1] Length = 523 Score = 193 bits (491), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 153/530 (28%), Positives = 248/530 (46%), Gaps = 25/530 (4%) Query: 3 NWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPTVQ---TACELGFGQGLSSNIHA 59 W+ GYV I Y G++P +P L A G++ + T ELG GQGL+S + A Sbjct: 2 QWSSGYVHGIDYPVGFFPEQSPVHLSFACLAQGVEPVPLDREFTYMELGCGQGLTSTVLA 61 Query: 60 AASTT-RWWGNDFNPRQAAFAQELAASSG-SGAQLEDSSFSRFCRRD-DLPDFDFIALHG 116 AA+ R++ DF P Q AQ++A+++G + + SF + R + DLP FDF+ ++G Sbjct: 62 AANPQGRFYAVDFLPTQVRTAQDMASAAGIDNLTVLECSFEQMARGEIDLPAFDFVTMYG 121 Query: 117 IWSWVSQENRDIIFDFIDRKLKPGGVLYISYNTQPGWSAFMPLRELLVQHTQQSGNEEQG 176 ++SWV + NR I DF+ R LKPGG++Y++YN PGWS+ + L+ +L + + + Q Sbjct: 122 VYSWVDRTNRGHILDFLRRYLKPGGIVYLNYNALPGWSSALSLQRMLREFGRHDMCDRQ- 180 Query: 177 LPTAARIDAALAFAARLFEADPVYAQANPFMRDRLKVLQQQSRSYLAHEYFNRDWHVESF 236 AL A A A +R RL+ L+ YLAHEY N+ W Sbjct: 181 --AQLHQGGALVRALSQAGAAAFGAAEATGLRRRLRALESDEPGYLAHEYLNQGWDALFS 238 Query: 237 ADMHRRFSGAGLEFACPAHYVDHLDMANLTPAQRQQLYEITDPVLQQSVRDFMVNQQFRR 296 D+ + + A L++ A L+ AQ+ L + + +++ V D + N FR Sbjct: 239 IDVIQDLAQAKLDYVGSAELAYGFPELYLSHAQQSLLAQQPNAGMRELVWDQICNSSFRE 298 Query: 297 DYWVRGAQALDDRQRVQALALQSVILLTEPDKVPLTIQNVASEITLNRLIYEPILQALSD 356 D +VRGA+ + QR L + L P ++ L + + + Y+P++ AL Sbjct: 299 DIYVRGARRMSPHQRDAWLERCGLALTEAPSRITLELPLAIGNVRADAHTYQPVMDALCQ 358 Query: 357 YQPHTLVDLAA--SLAHRNLNLAQVLDSVLMLIGTGHAAPLPAEPDAAARAQIERRTGDL 414 +PH+ +LAA +L + L ++ ++L + AAP +A AQ + L Sbjct: 359 -RPHSFAELAALPALRKQGRGLREIASMAILLSASRQAAPF----FLSAAAQPRQAAQRL 413 Query: 415 NAWLLQRAALASPAGEQGEEGEITHLSSPVIGGGVRVDRVEQLFLLARQSCGDDVDAWPA 474 N + AG E L+SP++G G+ ++++ + W Sbjct: 414 NRLI---------AGWSAESDCFQALASPLLGNGLPSGLLQRVAFDQLSQAQSQPEQWVL 464 Query: 475 QVWRYIAAQGKRLLREGVRLEGEAENLAELATQARLFARTRLPLLKALGV 524 + + I QG L R G L E AEL A+ RLP+ L + Sbjct: 465 PMAQAIHRQGTHLQRGGAPLSTREEIHAELLALAQAICTQRLPVWANLNM 514 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,608,733 Number of extensions: 66383 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 191 Number of HSP's successfully gapped: 2 Length of query: 525 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 435 Effective length of database: 1,193,720 Effective search space: 519268200 Effective search space used: 519268200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)