BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0027.p (249 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 489 e-140 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 410 e-116 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 153 2e-38 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 141 5e-35 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 137 6e-34 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 489 bits (1260), Expect = e-140, Method: Compositional matrix adjust. Identities = 245/249 (98%), Positives = 247/249 (99%) Query: 1 MLLKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGVQHLGLVA 60 MLLKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLG QHLGLVA Sbjct: 1 MLLKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLGLVA 60 Query: 61 DVTDKASCEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMS 120 DVTDKA+CEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMS Sbjct: 61 DVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMS 120 Query: 121 QAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGIRV 180 QAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGIRV Sbjct: 121 QAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGIRV 180 Query: 181 NCVTPGLISTDINKDILSAEKKTEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGVTL 240 NC+TPGLISTDINKDILSAEKK EIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGVTL Sbjct: 181 NCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGVTL 240 Query: 241 DVNGGMLIH 249 DVNGGMLIH Sbjct: 241 DVNGGMLIH 249 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 410 bits (1054), Expect = e-116, Method: Compositional matrix adjust. Identities = 208/249 (83%), Positives = 228/249 (91%) Query: 1 MLLKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGVQHLGLVA 60 MLLKDKVA+ITGGAG NGLG++TARLMA QGAR+V+LDL +A+PA AAAR+G QHLGLVA Sbjct: 1 MLLKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVA 60 Query: 61 DVTDKASCEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMS 120 DVTDKASCE AAAA+L+QFGRIDILVNNAG+TQP K LEITG DYDRILDVSLRGTLYMS Sbjct: 61 DVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLYMS 120 Query: 121 QAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGIRV 180 QAVLPAM+AQQ+GSI+CISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGP+GIRV Sbjct: 121 QAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEGIRV 180 Query: 181 NCVTPGLISTDINKDILSAEKKTEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGVTL 240 N +TPGLI TDI K L+ EKK EIA+TIPL RLG DD+GG CVFL SDLSQYCTG+TL Sbjct: 181 NSITPGLIGTDIIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTGITL 240 Query: 241 DVNGGMLIH 249 DVNGGMLIH Sbjct: 241 DVNGGMLIH 249 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 153 bits (386), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 98/252 (38%), Positives = 147/252 (58%), Gaps = 13/252 (5%) Query: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAA-----ARLGVQH-L 56 L+ +VA++TG + G+G + A +A QGA+++ +E AA A LG + Sbjct: 6 LQGQVALVTGAS--RGIGRAIATELARQGAKVI--GTATSESGAAAITEYLAALGPEAGR 61 Query: 57 GLVADVTDKASCEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGT 116 G V +V D + A V K FG + ILVNNAG+TQ + + +++D ++ +L Sbjct: 62 GAVLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAV 121 Query: 117 LYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQ 176 +S+AVL M ++G I+ I+SV + G G +Y+AAKAGV G++RA+ARE G + Sbjct: 122 GRLSRAVLRGMMKARTGRIINITSVVGEAGNP--GQMNYAAAKAGVAGMSRALAREIGSR 179 Query: 177 GIRVNCVTPGLISTDINKDILSAEKKTEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCT 236 I VNCV PG I TD+ + L+ ++ I Q IPLGRLG +++ FLAS + Y T Sbjct: 180 NITVNCVAPGFIDTDMTR-ALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYIT 238 Query: 237 GVTLDVNGGMLI 248 G TL+VNGGM + Sbjct: 239 GTTLNVNGGMYM 250 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 141 bits (356), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 79/199 (39%), Positives = 124/199 (62%), Gaps = 3/199 (1%) Query: 52 GVQHLGLVADVTDKASCEAAAAAVLKQFGRIDILVNNAG-VTQPAKLLEITGKDYDRILD 110 G + + + ADV D ++ AA A + Q GRID+L+NNAG + + A L +T + +D I++ Sbjct: 55 GSKSITIGADVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIIN 114 Query: 111 VSLRGTLYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMA 170 ++ R + ++AV+P M+AQ G+I+ ++SV+A+ GGG G Y+A+K V L R MA Sbjct: 115 INARSVVAFTRAVIPVMRAQGGGNIINVTSVAARHGGG-PGALIYAASKGFVSTLTRGMA 173 Query: 171 REFGPQGIRVNCVTPGLISTDINKDILSAEKKTEIAQTIPLGRLGGPDDVGGVCVFLASD 230 +E IRVN V PG+I T + + E+ +TIP+GR+G PD+ G ++LAS+ Sbjct: 174 KELLADKIRVNAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASE 233 Query: 231 -LSQYCTGVTLDVNGGMLI 248 +S Y TG +DVNGG + Sbjct: 234 QMSGYVTGQIIDVNGGQYM 252 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 137 bits (346), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 95/248 (38%), Positives = 138/248 (55%), Gaps = 12/248 (4%) Query: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAAR-LGVQHLGLVAD 61 +DKV ++TGG+ G+G +TA+L AA+GAR+ + RA+ A R +G +G+ D Sbjct: 7 FEDKVVVVTGGS--EGIGLATAKLFAAEGARVYVSG-RRADKLEQAVREIGNGAVGVPGD 63 Query: 62 VTDKASCEAAAAAVLKQFGRIDILVNNAGV-TQPAKLLEITGK-DYDRILDVSLRGTLYM 119 + A V GR+D+L NAG + AK L + G+ D+D + ++++RG L+ Sbjct: 64 AAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLFS 123 Query: 120 SQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGIR 179 Q LP M Q G+IV SV+ +G G Y+A+KA V AR+ + +GIR Sbjct: 124 VQKALPLMS--QGGAIVLNGSVAGVKG--FPGQSLYNASKAAVRSFARSWTADLKERGIR 179 Query: 180 VNCVTPGLISTDINKDILSAEKKTE--IAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTG 237 VN V PG T + +D L A E + Q +PLGRLG PD++G +FLAS S Y G Sbjct: 180 VNVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLASAESSYIAG 239 Query: 238 VTLDVNGG 245 V L V+GG Sbjct: 240 VELFVDGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 817,321 Number of extensions: 35803 Number of successful extensions: 318 Number of sequences better than 1.0e-08: 51 Number of HSP's gapped: 203 Number of HSP's successfully gapped: 51 Length of query: 249 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 166 Effective length of database: 1,226,865 Effective search space: 203659590 Effective search space used: 203659590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)