BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0021.p (664 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum s... 1355 0.0 gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum s... 555 e-159 gi|300309645|ref|YP_003773737.1| transketolase N-terminal subuni... 121 2e-28 gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [He... 105 1e-23 gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subun... 60 4e-10 >gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 1355 bits (3508), Expect = 0.0, Method: Compositional matrix adjust. Identities = 661/664 (99%), Positives = 662/664 (99%) Query: 1 MNEHPLQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRFNPAAPTWWDRD 60 MNEHPLQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRFNPAAPTWWDRD Sbjct: 1 MNEHPLQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRFNPAAPTWWDRD 60 Query: 61 RVVLSNGHGSMLLYCLLYLTGYEHITLEQVRSFREFGSHCAGHPEYAPELGIEVTTGPLG 120 RVVLSNGHGSMLLYCLLYLTGYEHITLEQVRSFREFGSHCAGHPEYAPELGIEVTTGPLG Sbjct: 61 RVVLSNGHGSMLLYCLLYLTGYEHITLEQVRSFREFGSHCAGHPEYAPELGIEVTTGPLG 120 Query: 121 QGIANAFGMAVAEAYLNARFGDDIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTL 180 QGIANAFGMAVAEAYLNARFGDDIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTL Sbjct: 121 QGIANAFGMAVAEAYLNARFGDDIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTL 180 Query: 181 LWDDNQITDDGKTELSISENVAERFRVAGWHVVEVDGHDIQAVSRALDAAQASDRPSMIA 240 LWDDNQITDDGKTELSISENVAERFRVAGWHVVEVDGHDIQAVSRALDAAQ SDRPSMIA Sbjct: 181 LWDDNQITDDGKTELSISENVAERFRVAGWHVVEVDGHDIQAVSRALDAAQGSDRPSMIA 240 Query: 241 CRTVIGRGIPRVQGQRGGHSARLYEDDAQAARELLQWPHAPFDVPQDILAQWRSFGQRND 300 CRTVIGRGIPRVQGQRGGHSARLYEDDAQAARELLQWPHAPFDVPQDILAQWRSFGQRND Sbjct: 241 CRTVIGRGIPRVQGQRGGHSARLYEDDAQAARELLQWPHAPFDVPQDILAQWRSFGQRND 300 Query: 301 AQHQQWQARVQALPAAQRAEFERVMRGELPSGWQEVLLPFKRRAHEPEQAQGGILISAAI 360 AQHQQWQARVQALPAAQRAEFERVMRGELPSGWQEVLLPFKR AHEPEQAQGGILISAAI Sbjct: 301 AQHQQWQARVQALPAAQRAEFERVMRGELPSGWQEVLLPFKRSAHEPEQAQGGILISAAI 360 Query: 361 NKLLAEVMPERMVGCADLEAPTAHKGGLHAFTADDRSGAYVHCGVREHLMGAMANGMAAH 420 NKLLAEVMPERMVGCADLEAPTAHKGGLHAFTADDRSGAYVHCGVREHLMGAMANGMAAH Sbjct: 361 NKLLAEVMPERMVGCADLEAPTAHKGGLHAFTADDRSGAYVHCGVREHLMGAMANGMAAH 420 Query: 421 GGVIALSVTYLAFSDYERPAMRMSALMGLPVKYVFSHDSIGIGKNGPTHQPVEILASLRA 480 GGVIALSVTYLAFSDYERPAMRMSALMGLPVKYVFSHDSIGIGKNGPTHQPVEILASLRA Sbjct: 421 GGVIALSVTYLAFSDYERPAMRMSALMGLPVKYVFSHDSIGIGKNGPTHQPVEILASLRA 480 Query: 481 MPNMLVMRPADATEAVECWEIAMQHRSGPVSMVFARQALPPVRRQYEAENRCARGGYVLH 540 MPNMLVMRPADATEAVECWEIAMQHRSGPVSMVFARQALPPVRRQYEAENRCARGGYVLH Sbjct: 481 MPNMLVMRPADATEAVECWEIAMQHRSGPVSMVFARQALPPVRRQYEAENRCARGGYVLH 540 Query: 541 EAQCGARQVTLLATGSEVALACAARAQLEAAGIATAVVSLPCWELFDQQDTAYRQQVLGE 600 EAQCGARQVTLLATGSEVALACAARAQLEAAGIATAVVSLPCWELFDQQDTAYRQQVLGE Sbjct: 541 EAQCGARQVTLLATGSEVALACAARAQLEAAGIATAVVSLPCWELFDQQDTAYRQQVLGE 600 Query: 601 QTVRVGVEAALRFGWDHYLGARGGFVGMPGFGAPGPADVLYRHFNITAEAVVAEARRLLL 660 QTVRVGVEAALRFGWDHYLGARGGFVGMPGFGAPGPADVLYRHFNITAEAVVAEARRLL+ Sbjct: 601 QTVRVGVEAALRFGWDHYLGARGGFVGMPGFGAPGPADVLYRHFNITAEAVVAEARRLLV 660 Query: 661 QGTH 664 QGTH Sbjct: 661 QGTH 664 >gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 555 bits (1429), Expect = e-159, Method: Compositional matrix adjust. Identities = 294/661 (44%), Positives = 405/661 (61%), Gaps = 9/661 (1%) Query: 5 PLQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRFNPAAPTWWDRDRVVL 64 P +A+AIR L++DA+ +A GH G+P+GMAEIA AL+A+H R NPA P W +RDR VL Sbjct: 6 PTDKMANAIRALAMDAVQKANSGHPGMPMGMAEIAVALWAKHYRHNPANPHWANRDRFVL 65 Query: 65 SNGHGSMLLYCLLYLTGYEHITLEQVRSFREFGSHCAGHPEYAPELGIEVTTGPLGQGIA 124 SNGHGSML Y LL+LTGY+ +++E ++SFR+ S AGHPE G+E TTGPLGQGI Sbjct: 66 SNGHGSMLQYALLHLTGYD-LSMEDIKSFRQLHSKTAGHPEVHITPGVETTTGPLGQGIT 124 Query: 125 NAFGMAVAEAYLNARF---GDDIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTLL 181 NA GMA+AE L A F G +V+H TYAFVGDGCL EGI E SLAG L L KL +L Sbjct: 125 NAVGMALAEKLLAAEFNKPGLAVVDHYTYAFVGDGCLMEGISHEACSLAGVLNLSKLIVL 184 Query: 182 WDDNQITDDGKTELSISENVAERFRVAGWHVV-EVDGHDIQAVSRALDAAQASDRPSMIA 240 +DDN I+ DG E ++ +RF GW+V+ VDGH ++AVS A+ A+ +D+P++I Sbjct: 185 YDDNGISIDGHVEGWFKDDTPKRFEAYGWNVIPAVDGHSVEAVSAAIHQAKLADKPTLIC 244 Query: 241 CRTVIGRGIPRVQGQRGGHSARLYEDDAQAARELLQWPHAPFDVPQDILAQW--RSFGQR 298 CRTVIG+G P + G H A L + + A RE L W APF++P D+ + W ++ G Sbjct: 245 CRTVIGKGSPNLAGTDKVHGAALGDKEIAAVREALGWTSAPFEIPADVYSAWDAKATGAD 304 Query: 299 NDAQHQQWQARVQALPAAQRAEFERVMRGELPSGWQEVLLPFKRRAHEPEQAQGGILISA 358 +A +Q A ++ AE R ++GELP+ ++ + + E ++ S Sbjct: 305 FEASWKQTFDTYAAQYPSEAAELLRRLKGELPAAFESTVTAYINSTLEKKETIATRKASQ 364 Query: 359 AINKLLAEVMPERMVGCADLEAPTAHKGGLHAFTADDRSGAYVHCGVREHLMGAMANGMA 418 + A+ +PE + G ADL D+ G +++ GVRE M A+ NG+A Sbjct: 365 NAIQAYAQALPEFLGGSADLTGSNLTNWKESVAVRADQRGNHINYGVREFGMSAIMNGIA 424 Query: 419 AHGGVIALSVTYLAFSDYERPAMRMSALMGLPVKYVFSHDSIGIGKNGPTHQPVEILASL 478 HGG + T+L FSDY R A+RM+ALM + +VF+HDSIG+G++GPTHQ VE ++SL Sbjct: 425 LHGGYLPFGATFLTFSDYSRNALRMAALMKIRALFVFTHDSIGLGEDGPTHQSVEHVSSL 484 Query: 479 RAMPNMLVMRPADATEAVECWEIAMQHRSGPVSMVFARQALPPVRRQYEAENRCARGGYV 538 R +PN+ RP D TE W +++ GP +++F+RQ LP R ARGGYV Sbjct: 485 RLIPNLDNWRPCDTTETAVAWAESIKRHDGPSTLIFSRQNLPFQERTDAQVKDIARGGYV 544 Query: 539 LHEAQCGARQVTLLATGSEVALACAARAQLEAAGIATAVVSLPCWELFDQQDTAYRQQVL 598 L +A +V L+ATGSE+ LA A L A GIA VVS+P ++FD+QD AY+ VL Sbjct: 545 LRDAPNA--KVILMATGSEIELATKAADALTAEGIAVRVVSMPSTDVFDRQDAAYKASVL 602 Query: 599 GEQTVRVGVEAALRFGWDHYLGARGGFVGMPGFGAPGPADVLYRHFNITAEAVVAEARRL 658 RV +EA + W Y+G G VG+ FG PA VL++HF T E VVA+A+ + Sbjct: 603 TRGVPRVAIEAGVTAFWHKYVGLEGAVVGIDTFGESAPAGVLFKHFGFTVENVVAKAKAV 662 Query: 659 L 659 L Sbjct: 663 L 663 >gi|300309645|ref|YP_003773737.1| transketolase N-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 272 Score = 121 bits (304), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 91/236 (38%), Positives = 119/236 (50%), Gaps = 16/236 (6%) Query: 23 RATEGHQGVPLGMAEIATALY-ARHLRFNPAAPTWWDRDRVVLSNGHGSMLLYCLLYLTG 81 RA E L +I ALY LRFNPA P RDR V+S GHGS+ +Y +L G Sbjct: 25 RAPETRLASSLSSIDIFVALYYGGVLRFNPADPRDAGRDRCVISKGHGSICMYPILADLG 84 Query: 82 YEHITLEQVRSFREFGSHCAGHPEYAPEL-GIEVTTGPLGQGIANAFGMAVAEAYLNARF 140 Y +E+++ E GS G P+ P + G E G LG G+ A GMA+ R Sbjct: 85 Y--FPMEELQHVCEAGSFLGGIPD--PVIPGYETVNGSLGHGLGVATGMALGL----KRS 136 Query: 141 GDDIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTLLWDDNQITDDGKTELSISE- 199 G D + GDG L EG E I A +L L L+ DDN+I+ G TE +S Sbjct: 137 GSD---RSVFVVAGDGELHEGANWEAIMFASQHKLDNLHLIVDDNRISMLGYTEDIVSHG 193 Query: 200 NVAERFRVAGWHVVEVDGHDIQAVSRAL--DAAQASDRPSMIACRTVIGRGIPRVQ 253 +A R GW +EVDGHD+ AV AL A A+ +P I RT+ G G+P ++ Sbjct: 194 ELATRLSAFGWDCMEVDGHDVLAVQAALLQQKAHAAGKPKAIIARTLKGHGVPGLE 249 >gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [Herbaspirillum seropedicae SmR1] Length = 281 Score = 105 bits (261), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 80/255 (31%), Positives = 125/255 (49%), Gaps = 16/255 (6%) Query: 26 EGHQGVPLGMAEIATALYARHLRFNPAAPTWWDRDRVVLSNGHGSMLLYCLLYLTGYEHI 85 +G+ G LG A++ Y + P P W RDR +LS+GH ++ LY L G + Sbjct: 28 QGYIGQALGWADVLAVAYKHAMSLRPDDPQWSGRDRFLLSHGHYAIALYAALIEAGI--V 85 Query: 86 TLEQVRSFREFGSHC--AGHPEYAPELGIEVTTGPLGQGIANAFGMAVAEAYLNARFGDD 143 E++ S+ S +G Y P G+E++ G LGQG+A GMA+ Y N Sbjct: 86 PDEELDSYGSDDSRLPMSGMATYTP--GMEISGGSLGQGLAIGVGMALGLRYNNNP---- 139 Query: 144 IVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTLLWD-DNQITDDGKTELSISENVA 202 Y + DG L EG E A H +L L L D +NQ D ++ E +A Sbjct: 140 ---AFVYNSMSDGELDEGSTWEAAMGAAHHQLGNLICLVDINNQQADGPSGKIMGFEPLA 196 Query: 203 ERFRVAGWHVVEVDGHDIQAVSRALDAAQ--ASDRPSMIACRTVIGRGIPRVQGQRGGHS 260 +++ GW+V VDG+D+ AV +A D A+ +P +I T++G+G+P ++ + H Sbjct: 197 DKWAAFGWYVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFDTLMGKGVPFLEQREKNHF 256 Query: 261 ARLYEDDAQAARELL 275 R+ + Q A +L Sbjct: 257 IRVDPPEWQQALSIL 271 >gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 334 Score = 60.5 bits (145), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 69/243 (28%), Positives = 107/243 (44%), Gaps = 16/243 (6%) Query: 350 AQGGILISAAINKLLAEVMPER--MVGCADLEAPTAHKGGLHAFTADDRSGAYVHCGVRE 407 A+G + +A K L + +R +VG L A A LH F + G+ E Sbjct: 22 AEGQRVQAAPFGKALVDYAADRPEIVG---LTADLAKYTDLHLFQ-KAYPERFFQMGMAE 77 Query: 408 HLMGAMANGMAAHGGVIALSVTYLAFSDYERPAM--RMSALMGLPVKYVFSHDSIGIGKN 465 L+ A A GMA G ++ + TY F+ ++ A L VK + + G Sbjct: 78 QLLMAAAGGMAKEG-LVPFATTYAVFATRRAYDFIHQVIAEENLNVKICCALPGLTTG-Y 135 Query: 466 GPTHQPVEILASLRAMPNMLVMRPADATEAVECWEIAMQHRSGPVSMVFARQALPPVRRQ 525 GP+HQ E LA +RA+P M ++ P DA + + H+ GPV M AR +P V + Sbjct: 136 GPSHQATEDLAMMRAIPGMTIVDPCDALDLEQAVPQIAAHQ-GPVYMRLARGNVPLVLDE 194 Query: 526 YEAENRCARGGYVLHEAQCGARQVTLLATGSEVALACAARAQLEAAGIATAVVSLPCWEL 585 Y+ R E + G V ++++G A LEA ++ AV+ P + Sbjct: 195 YDYRFELGRA----KEIRDG-NDVLIISSGFMTMRALEVAKALEADKVSVAVLHCPTIKP 249 Query: 586 FDQ 588 FD+ Sbjct: 250 FDE 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,158,371 Number of extensions: 94310 Number of successful extensions: 329 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 319 Number of HSP's successfully gapped: 5 Length of query: 664 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 573 Effective length of database: 1,188,985 Effective search space: 681288405 Effective search space used: 681288405 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)