BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0001.p (485 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 947 0.0 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 422 e-119 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 407 e-115 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 374 e-104 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 236 3e-63 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 947 bits (2449), Expect = 0.0, Method: Compositional matrix adjust. Identities = 479/485 (98%), Positives = 480/485 (98%), Gaps = 2/485 (0%) Query: 1 MANLSMPRSPRLLHMCAALVVAGVLGGCSLAPTYERPQAPVESSYPADAAGRAELQAVNL 60 MANLSMPRSPRLLHMCAALV+AGVLGGCSLAPTYERPQAPVESSYPADAAGRAELQAVNL Sbjct: 1 MANLSMPRSPRLLHMCAALVMAGVLGGCSLAPTYERPQAPVESSYPADAAGRAELQAVNL 60 Query: 61 GWRQFFPDQRLQQLIAAALENNRDLRTAVLNIELARARAQYQIARADLLPNLNLSGNAAR 120 GWRQFFPDQRLQQLIAAALENNRDLRTAVLNIE ARAQYQIARADLLPNLNLSGNAAR Sbjct: 61 GWRQFFPDQRLQQLIAAALENNRDLRTAVLNIE--EARAQYQIARADLLPNLNLSGNAAR 118 Query: 121 TRTAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQIS 180 TRTAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQIS Sbjct: 119 TRTAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQIS 178 Query: 181 LVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGTSTALDFRLTQTLVESA 240 LVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVG STALDFRLTQTLVESA Sbjct: 179 LVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESA 238 Query: 241 RVAKTQLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIR 300 RVAKTQLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIR Sbjct: 239 RVAKTQLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIR 298 Query: 301 SAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFD 360 SAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFD Sbjct: 299 SAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFD 358 Query: 361 FGRNSANLDLAEVRKNKAVVTYEKTIQTAFREVADALVARGSLDEQVKAQEAFLAAQQER 420 FGRNSANLDLAEVRKNKAVVTYEK IQTAFREVADALVARGSLDEQVKAQEAFLAAQQER Sbjct: 359 FGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQER 418 Query: 421 LKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENTVS 480 LKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENTVS Sbjct: 419 LKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENTVS 478 Query: 481 VAAKQ 485 VAAKQ Sbjct: 479 VAAKQ 483 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 422 bits (1086), Expect = e-119, Method: Compositional matrix adjust. Identities = 246/489 (50%), Positives = 318/489 (65%), Gaps = 19/489 (3%) Query: 8 RSPRLLHMCAALVVAGVLGGCSLAPTYERPQAPVESSYPA----DAAGRAELQAV-NLGW 62 RSP +L M +++A +LGGC+L P Y RP APV + + +AAG + V ++GW Sbjct: 8 RSP-VLPMMLPVMLAMLLGGCTLMPDYHRPDAPVAAHFAGADSKNAAGASSAPVVADIGW 66 Query: 63 RQFFPDQRLQQLIAAALENNRDLRTAVLNIELARARAQYQIARADLLPNLNLSGNAARTR 122 R F D LQQ+IA ALENNRDLR AVLNIE +ARAQY + RA L P++ + + +R Sbjct: 67 RDVFTDPSLQQVIALALENNRDLRVAVLNIE--KARAQYGVQRAALFPSIKAATSETASR 124 Query: 123 TAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLV 182 T S TG S SY L AYELD FGRVR+LK+ AL S+LST EA++S QISL+ Sbjct: 125 TPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLI 184 Query: 183 AQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGTSTALDFRLTQTLVESARV 242 A+VA A+L S ++L+LA +TL+S+ S+ L Q+ ++G+S+AL R QT VESARV Sbjct: 185 AEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARV 244 Query: 243 AKTQLERQRAQAENALTLLVGKKIGELPPAQL---LSDEKI-----VTDIPAGLPSDLLT 294 Q Q NAL LLVGK +LP A L L D ++ + IP GLPSDLL Sbjct: 245 DVESYTAQVDQDRNALVLLVGK---DLPTALLPQGLPDTRLAAASPLATIPPGLPSDLLQ 301 Query: 295 NRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSL 354 R DI AE+ L AANANIGAARAAF+P I+LT N G+AS+ L GLF+ GSG+WSF P + Sbjct: 302 RRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQI 361 Query: 355 SLPIFDFGRNSANLDLAEVRKNKAVVTYEKTIQTAFREVADALVARGSLDEQVKAQEAFL 414 SLPIFD G N ANLD+A V ++ +V YEK IQTAFREV+DAL R +L Q++AQEA + Sbjct: 362 SLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALV 421 Query: 415 AAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGL 474 A E +L+ R+ +G+ S L LD+QR L+SAQ+ LI + R N + YK LGGG Sbjct: 422 QASDEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGGW 481 Query: 475 NENTVSVAA 483 E T + AA Sbjct: 482 VERTGTPAA 490 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 407 bits (1047), Expect = e-115, Method: Compositional matrix adjust. Identities = 229/464 (49%), Positives = 304/464 (65%), Gaps = 14/464 (3%) Query: 27 GCS-LAPTYERPQAPVESSYPADAAGR----------AELQAVNLGWRQFFPDQRLQQLI 75 GC+ LAP Y +P APV +S+ + AA + E+ A +LGWR+FF D RLQQ+I Sbjct: 27 GCANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVI 86 Query: 76 AAALENNRDLRTAVLNIELARARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQPTI 135 AAAL+NNRDLR A LNIE +ARAQY+I A+ P+++ +G ++ +RT A S TG + Sbjct: 87 AAALDNNRDLRVAALNIE--KARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAV 144 Query: 136 SNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAERSY 195 S+ V + V AYELDFFGR++NL DAAL SY E ++S ISLVA+VA A+L S Sbjct: 145 SHQQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASD 204 Query: 196 AEQLKLADETLKSRQSNLDLAQKRLDVGTSTALDFRLTQTLVESARVAKTQLERQRAQAE 255 + LKLA +T S+ L+Q+ D+GT + +D + VESAR + Q Q Sbjct: 205 RDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDL 264 Query: 256 NALTLLVGKKI-GELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIG 314 NAL LLVG I L PA L ++ PAGLPS +L RPD+ +AE +L +ANA+IG Sbjct: 265 NALRLLVGADIDAALLPAGLPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIG 324 Query: 315 AARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVR 374 AARAAFFP ISLT N G+AS TLGGLF++GSG+W+F PS++LPIF+ G A+LD A++ Sbjct: 325 AARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKIT 384 Query: 375 KNKAVVTYEKTIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIAS 434 ++ V YEK IQTAFREVADAL R +LD+++ AQ AA ++ +L+D R++NG+ S Sbjct: 385 RDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDS 444 Query: 435 SLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENT 478 L L AQR L+SAQQ LI R +N I LYK LGGG +E T Sbjct: 445 YLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWSEQT 488 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 374 bits (959), Expect = e-104, Method: Compositional matrix adjust. Identities = 217/453 (47%), Positives = 299/453 (66%), Gaps = 11/453 (2%) Query: 28 C-SLAPTYERPQAPVESSYPADAAGRAELQAVN------LGWRQFFPDQRLQQLIAAALE 80 C SLAP Y+RP APV + YP A Q N L WR +F D LQ LI ALE Sbjct: 25 CQSLAPHYQRPAAPVAAVYPDGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQALE 84 Query: 81 NNRDLRTAVLNIELARARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQPTISNSYQ 140 N+RDLRTA L ++ A+A Y I ADL P + R+RT A +LT +P + ++YQ Sbjct: 85 NSRDLRTAALRVQ--EAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQ 142 Query: 141 VGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAERSYAEQLK 200 GL + ++E+D +GR+R+L DAAL S +++ + + +SL+AQVA A+L E+L+ Sbjct: 143 AGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLE 202 Query: 201 LADETLKSRQSNLDLAQKRLDVGTSTALDFRLTQTLVESARVAKTQLERQRAQAENALTL 260 +A++T+ SRQ + + +R++VG ++ L+ +TL+ A+ QL++QRAQ NALTL Sbjct: 203 IANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTL 262 Query: 261 LVGKKIGELPPAQLLSD-EKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAA 319 LVG + LP Q + + + + GLP+ LLT RPD+ +AE L+AANANIGAARAA Sbjct: 263 LVGSPV-VLPVGQARAGLDAVFAPLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAA 321 Query: 320 FFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAV 379 FFP ISLTGN G+AS+ L GLF GS AW+F PS++LPIF GR NL L+EVR++ AV Sbjct: 322 FFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAV 381 Query: 380 VTYEKTIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQL 439 YEKT+QTAFREVADAL AR L +Q++ ++ + Q+ER +L+ LR+ NG A LD L Sbjct: 382 AAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVL 441 Query: 440 DAQRDLFSAQQALIQARQLRLNNAIDLYKALGG 472 DAQRDL SAQQ L+Q R+ L++++ LY ALGG Sbjct: 442 DAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 236 bits (602), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 173/480 (36%), Positives = 260/480 (54%), Gaps = 13/480 (2%) Query: 15 MCAALVVAGVLGGCSLAP-TYERPQAPVE--SSYP----ADAAGRAELQAVNLGWRQFFP 67 M L + L C+ AP T P + ++ + YP DA A A +GW+ +FP Sbjct: 5 MSRHLRLLPTLALCACAPLTAPLPPSALDLPTHYPRTLAEDAPANAAPTASAIGWQDYFP 64 Query: 68 DQRLQQLIAAALENNRDLRTAVLNIELARARAQYQIARADLLPNLNLSGNAARTRTAASQ 127 LQ LIA AL +N DLR + + L + +AQ++I R+ LP +NL A R+R Sbjct: 65 QAPLQALIAEALRHNPDLRD--IRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEP 122 Query: 128 SLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQ 187 +G + Y+VGL ++ELDF+GR+ +L +AAL ++++ A+++ ISL+ QVA Sbjct: 123 PESGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAA 182 Query: 188 AYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGTSTALDFRLTQTLVESARVAKTQL 247 A+ + R E+L L + + +L L Q R D G + A ++ + R + +L Sbjct: 183 AWTSLRELDERLHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMRAEQAEL 242 Query: 248 ERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTD--IPAGLPSDLLTNRPDIRSAEQS 305 ++ RA + L +L G + +LP A + I+ + GLPS LL RPDI +AE Sbjct: 243 QQMRASQRSTLEMLAG--LTQLPAALEPAPTTILLPFALAPGLPSTLLAARPDIIAAEHR 300 Query: 306 LLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNS 365 L A+ A + AARAAFFPRISLT G AS+ L LF+ S AW+F P +SLP+ D G Sbjct: 301 LQASAAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQ 360 Query: 366 ANLDLAEVRKNKAVVTYEKTIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTD 425 + D+A + + +A++ YE+T+Q AFREVA L L EQ++ + + Q +R Sbjct: 361 SRYDIAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMH 420 Query: 426 LRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENTVSVAAKQ 485 +R + G AS LD L+++RD AQQ L N I LY ALGGG + S ++Q Sbjct: 421 MRLRYGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGGTQALSASSPSQQ 480 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.347 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,238,638 Number of extensions: 45930 Number of successful extensions: 297 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 240 Number of HSP's successfully gapped: 17 Length of query: 485 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 396 Effective length of database: 1,198,455 Effective search space: 474588180 Effective search space used: 474588180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)