BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0040.p (543 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313221|ref|YP_003777313.1| periplasmic glucans biosynthesi... 1098 0.0 gi|300312745|ref|YP_003776837.1| periplasmic glucans biosynthesi... 347 1e-96 gi|300314061|ref|YP_003778153.1| periplasmic glucan biosynthesis... 300 1e-82 >gi|300313221|ref|YP_003777313.1| periplasmic glucans biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 544 Score = 1098 bits (2841), Expect = 0.0, Method: Compositional matrix adjust. Identities = 541/543 (99%), Positives = 541/543 (99%) Query: 1 MTSRRRFLCATGATATAACLSALGLPSGALAAQGIETSAPQPFSFAQLIERARQMASTPY 60 MTSRRRFLCATGATATAA LSALGLPSGALAAQGIETSAPQPFSFAQLIERARQMASTPY Sbjct: 2 MTSRRRFLCATGATATAAALSALGLPSGALAAQGIETSAPQPFSFAQLIERARQMASTPY 61 Query: 61 QPAAAAPAAILDRIDYDAHGKIRFKPDRAAFATGPGPFPLTFFHLGKFFRVPVRMFLLEG 120 QPAAAAPAAILDRIDYDAHGKIRFKPDRAAFATGPGPFPLTFFHLGKFFRVPVRMFLLEG Sbjct: 62 QPAAAAPAAILDRIDYDAHGKIRFKPDRAAFATGPGPFPLTFFHLGKFFRVPVRMFLLEG 121 Query: 121 AGDATAPTQARELRYRDDYFDMPADSPAHALPPGSGFAGFRFQESRLGDQQVKDWRKNDW 180 AGDATAPTQARELRYRDDYFDMPADSPAHALPPGSGFAGFRFQESRLGDQQVKDWRKNDW Sbjct: 122 AGDATAPTQARELRYRDDYFDMPADSPAHALPPGSGFAGFRFQESRLGDQQVKDWRKNDW 181 Query: 181 VAFLGASYFRAIGDLYQYGLSARGVAIDVAVAGKSEEFPDFTQFFFEPGAPGSDTVVVYA 240 VAFLGASYFRAIGDLYQYGLSARGVAIDVAVAGKSEEFPDFTQFFFEPGAPGSDTVVVYA Sbjct: 182 VAFLGASYFRAIGDLYQYGLSARGVAIDVAVAGKSEEFPDFTQFFFEPGAPGSDTVVVYA 241 Query: 241 LLDGPSITGAYRFAMQRGQGVTMEIDKQLFLRRDVERLGLAPATSMYWYSETVKGTGVDW 300 LLDGPSITGAYRFAMQRGQGVTMEIDKQLFLRRDVERLGLAPATSMYWYSETVKGTGVDW Sbjct: 242 LLDGPSITGAYRFAMQRGQGVTMEIDKQLFLRRDVERLGLAPATSMYWYSETVKGTGVDW 301 Query: 301 RPEVHDSDGLAIWNGAGEHLWRPLNNPPRTIASSFADRQPRGFGLMQRDRDFDHYLDGVH 360 RPEVHDSDGLAIWNGAGEHLWRPLNNPPRTIASSFADRQPRGFGLMQRDRDFDHYLDGVH Sbjct: 302 RPEVHDSDGLAIWNGAGEHLWRPLNNPPRTIASSFADRQPRGFGLMQRDRDFDHYLDGVH 361 Query: 361 YERRPSLWVEPLGDWGEGQVQLIELRTDDEIHDNIVAMWVPKAAARAGDRYHLRYRLHWQ 420 YERRPSLWVEPLGDWGEGQVQLIELRTDDEIHDNIVAMWVPKAAARAGDRY LRYRLHWQ Sbjct: 362 YERRPSLWVEPLGDWGEGQVQLIELRTDDEIHDNIVAMWVPKAAARAGDRYQLRYRLHWQ 421 Query: 421 SETPFPSALARCVATRLGRGGQPGQPRPPQVRKFMVEFKGPPLQALPFGVKPEAVISASR 480 SETPFPSALARCVATRLGRGGQPGQPRPPQVRKFMVEFKGPPLQALPFGVKPEAVISASR Sbjct: 422 SETPFPSALARCVATRLGRGGQPGQPRPPQVRKFMVEFKGPPLQALPFGVKPEAVISASR 481 Query: 481 GSISYVYTEALPNGVPGHWRAQFDLTAEGEEPVELRLFLRHQGQALSETWLFQYHPFIAR 540 GSISYVYTEALPNGVPGHWRAQFDLTAEGEEPVELRLFLRHQGQALSETWLFQYHPFIAR Sbjct: 482 GSISYVYTEALPNGVPGHWRAQFDLTAEGEEPVELRLFLRHQGQALSETWLFQYHPFIAR 541 Query: 541 EVY 543 EVY Sbjct: 542 EVY 544 >gi|300312745|ref|YP_003776837.1| periplasmic glucans biosynthesis G protein [Herbaspirillum seropedicae SmR1] Length = 541 Score = 347 bits (890), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 210/505 (41%), Positives = 290/505 (57%), Gaps = 30/505 (5%) Query: 43 FSFAQLIERARQMASTPYQ-PAAAAPAAILDRIDYDAHGKIRFKPDRAAFATGPGPFPLT 101 F F + RA+ +A Y+ PA P ++ D + Y+ +I F+ D++ + PF L+ Sbjct: 55 FDFNTVAARAKALAGKSYKKPADELPKSLKD-LSYEQASQIYFRDDKSYWRAQKLPFELS 113 Query: 102 FFHLGKFFRVPVRMFLLEGAGDATAPTQARELRYRDDYFDMPADSPAHALPPGS----GF 157 F HLG + P+++ E GDA RE+ + FD A ++ P S GF Sbjct: 114 FIHLGASYTEPIKVN--EVVGDAV-----REIHFNPALFDYSA---TRSVDPRSLRNLGF 163 Query: 158 AGFRFQESRLGDQQVKDWRKNDWVAFLGASYFRAIGDLYQYGLSARGVAIDVAVAGKSEE 217 AGFR + + +VKD D + F GASYFRA+G YGLSARG+AID A+ EE Sbjct: 164 AGFRVRFP-VNSAKVKD----DVLTFHGASYFRALGKGQSYGLSARGLAIDTALYS-GEE 217 Query: 218 FPDFTQFFFEPGAPGSDTVVVYALLDGPSITGAYRFAMQRGQGVTMEIDKQLFLRRDVER 277 FP FT+F+ E + S +V+YALLD P TGAYRF ++ G M++ QL+LR +V + Sbjct: 218 FPRFTEFWLERPSAESKELVIYALLDSPRATGAYRFVLKPGADTVMDVKAQLYLRSNVTK 277 Query: 278 LGLAPATSMYWYSETVKGTGVDWRPEVHDSDGLAIWNGAGEHLWRPLNNPPRTIASSFAD 337 LG+AP TSM+ + E G D+RPEVHDSDGLA+ +G GE LWRPL NP R + +SF+ Sbjct: 278 LGIAPLTSMFLFGENQPGPADDFRPEVHDSDGLAMQSGTGEWLWRPLVNPKRLLVTSFSF 337 Query: 338 RQPRGFGLMQRDRDFDHYLD-GVHYERRPSLWVEPLGDWGEGQVQLIELRTDDEIHDNIV 396 P GFGLMQRDR F HY D +Y+ RPS WVEP G WG G+V+L+++ T DE +DNIV Sbjct: 338 DNPLGFGLMQRDRSFSHYEDLDYNYQARPSAWVEPKGKWGSGRVELVQIPTPDETNDNIV 397 Query: 397 AMWVPKAAARAGDRYHLRYRLHWQSETPFPSALARCVATRLGRGGQPGQP-RPPQVRKFM 455 A W+P + G +++ YRL WQ + LA T G G +P Q + V++ Sbjct: 398 AYWIPNTLPKPGQPFNVEYRLSWQKDNEKRPPLAYVTQTLFGHGYRPKQEGKTDDVQQLS 457 Query: 456 VEFKGPPLQALPFGVKPEAVISA-SRGSISYVYTEALPNGVPGHWRAQFDL-TAEGEEPV 513 ++F G L+ LP + E V+ A + G ++ V T N G WR + L E +PV Sbjct: 458 IDFDGGNLKKLPANARVEGVVEADANGKLASVTTRK--NEATGGWRVEVKLRRTEENKPV 515 Query: 514 ELRLFLRHQ--GQALSETWLFQYHP 536 ELR FLR+ G LSETW + P Sbjct: 516 ELRGFLRNANGGTTLSETWSYILPP 540 >gi|300314061|ref|YP_003778153.1| periplasmic glucan biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 517 Score = 300 bits (769), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 190/525 (36%), Positives = 273/525 (52%), Gaps = 37/525 (7%) Query: 27 SGALAAQGIETSAPQPFSFAQLIERARQMASTPYQPAAAAPAAILDRIDYDAHGKIRFKP 86 S L A + Q F Q + RA+ +A+ PYQ + + + + + KI+ K Sbjct: 7 SACLLAASAQAQTSQGFDLNQAVARAKDLAAKPYQKPVSNLSPTFAAMQFADYMKIQPKS 66 Query: 87 DRAAFATGPGPFPLTFFHLGKFFRVPVRMF-LLEGAGDATAPTQARELRYRDDYFD---- 141 ++ + PF L F+H G F PV + +++G + +++ Y D F+ Sbjct: 67 EKFEWRDQKTPFKLAFYHQGMQFNAPVAIHEIIDG--------RVQDVGYSTDRFNFGDL 118 Query: 142 -MPADSPAHALPPGSGFAGFRFQESRLGDQQVKDWRKNDWVAFLGASYFRAIGDLYQYGL 200 + DS AH G+AGFR +D ++++ ++ LGASYFR IG YGL Sbjct: 119 NLAKDSTAHL-----GYAGFRVVYPI-----NRDDKEDEVMSVLGASYFRVIGKGQVYGL 168 Query: 201 SARGVAIDVAVAGKSEEFPDFTQFFFEPGAPGSDTVVVYALLDGPSITGAYRFAMQRGQG 260 SARG+AID A+ EEFP FT+F+ + APG + YALLD P TGAY+F + G+ Sbjct: 169 SARGLAIDTAMM-SGEEFPHFTEFWIQRPAPGEKQLTFYALLDSPRATGAYKFTLTPGKD 227 Query: 261 VTMEIDKQLFLRRDVERLGLAPATSMYWYSETVKGTGVDWRPEVHDSDGLAIWNGAGEHL 320 +++ ++FLR +V RLG+AP TSM+ + + + ++RP +HDS+GLAI G GE L Sbjct: 228 ALLDVQARVFLRGEVGRLGIAPLTSMFLFGPNQQSSRRNFRPAIHDSNGLAIHTGNGEWL 287 Query: 321 WRPLNNPPRTIASSFADRQPRGFGLMQRDRDFDHYLD-GVHYERRPSLWVEPLGDWGEGQ 379 WRPLN+P SSF PRGFGL+QR R+F Y D Y+ RPS W+EP GDWG+G Sbjct: 288 WRPLNDPQNVEVSSFEVSNPRGFGLLQRGREFSAYEDLKDRYDLRPSAWIEPKGDWGKGA 347 Query: 380 VQLIELRTDDEIHDNIVAMWVPKAAARAGDRYHLRYRLHWQSETP-FPSALARCVATRLG 438 VQL+E+ T DE +DNIVA W+P G YR+HW + P + V Sbjct: 348 VQLVEIPTADETNDNIVAYWLPAQLPARGTPLAYDYRIHWTMDEPAILNNEVGWVKQTFH 407 Query: 439 RGGQPGQPRPPQVRKF------MVEFKGPPLQALPFGVKPEAVISASRGSISYVYTEALP 492 G+ Q +R F +V+F G PL ALP G + +S S + + P Sbjct: 408 TDGELTQAN--LIRAFDGTTALLVDFTGGPLGALPAGTPVQPQVSVSNNG-ELIDVQLQP 464 Query: 493 NGVPGHWRAQFDLTAEG-EEPVELRLFLRHQGQALSETWLFQYHP 536 N WR + + VELR L G+ L+ETW +Q P Sbjct: 465 NPAINGWRLTLRVKIRNVAQAVELRAALSANGKPLTETWSYQLPP 509 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,834,470 Number of extensions: 85147 Number of successful extensions: 231 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 222 Number of HSP's successfully gapped: 3 Length of query: 543 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 453 Effective length of database: 1,193,720 Effective search space: 540755160 Effective search space used: 540755160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)