BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0027.p (456 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 937 0.0 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 238 5e-64 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 168 7e-43 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 142 8e-35 gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferas... 140 3e-34 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 937 bits (2421), Expect = 0.0, Method: Compositional matrix adjust. Identities = 455/456 (99%), Positives = 455/456 (99%) Query: 1 MIEAELESLNFADAPKMVTPTYPGPKTAAALELSARTESMARGGGRMPIAMDQAFGVTFK 60 MIEAELESLNF DAPKMVTPTYPGPKTAAALELSARTESMARGGGRMPIAMDQAFGVTFK Sbjct: 1 MIEAELESLNFTDAPKMVTPTYPGPKTAAALELSARTESMARGGGRMPIAMDQAFGVTFK 60 Query: 61 DPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSMEVNSSKRTELAAKISEIMP 120 DPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSMEVNSSKRTELAAKISEIMP Sbjct: 61 DPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSMEVNSSKRTELAAKISEIMP 120 Query: 121 DGLRGDCITFFTQGGSDALEAAVKFAKRVTGRHQIIAFHGGYHGIWNASNALTTGTAYRK 180 DGLRGDCITFFTQGGSDALEAAVKFAKRVTGRHQIIAFHGGYHGIWNASNALTTGTAYRK Sbjct: 121 DGLRGDCITFFTQGGSDALEAAVKFAKRVTGRHQIIAFHGGYHGIWNASNALTTGTAYRK 180 Query: 181 GFGPFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGEYVDYLLNTPYTAADDVAAVIVEPVQ 240 GFGPFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGEYVDYLLNTPYTAADDVAAVIVEPVQ Sbjct: 181 GFGPFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGEYVDYLLNTPYTAADDVAAVIVEPVQ 240 Query: 241 GEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAGRTGKMWAVEHSGVKPDMLTFGKG 300 GEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAGRTGKMWAVEHSGVKPDMLTFGKG Sbjct: 241 GEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAGRTGKMWAVEHSGVKPDMLTFGKG 300 Query: 301 IGGDMPMAGLVMRSDLAAKIPDGSQPNTFAANSISAAVALTNISILQDPRLDLVNRAHTL 360 IGGDMPMAGLVMRSDLAAKIPDGSQPNTFAANSISAAVALTNISILQDPRLDLVNRAHTL Sbjct: 301 IGGDMPMAGLVMRSDLAAKIPDGSQPNTFAANSISAAVALTNISILQDPRLDLVNRAHTL 360 Query: 361 GLEAQERIRSFNSPWVGEVRGRGLMIGIELVENRETREPLSREKLGKLMDYVVGHGVLMI 420 GLEAQERIRSFNSPWVGEVRGRGLMIGIELVENRETREPLSREKLGKLMDYVVGHGVLMI Sbjct: 361 GLEAQERIRSFNSPWVGEVRGRGLMIGIELVENRETREPLSREKLGKLMDYVVGHGVLMI 420 Query: 421 PCGRYTNVMRVMPSLTIPRSLMFKGLDIFGAGLASL 456 PCGRYTNVMRVMPSLTIPRSLMFKGLDIFGAGLASL Sbjct: 421 PCGRYTNVMRVMPSLTIPRSLMFKGLDIFGAGLASL 456 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 238 bits (608), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 155/429 (36%), Positives = 233/429 (54%), Gaps = 21/429 (4%) Query: 32 ELSARTESMA-RGGGRM-PIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEA 89 EL R + RG G M ++A D +G FID +AG+ V + G +P++++A Sbjct: 9 ELQQRKNAATPRGVGVMCDFYAERAANAELWDVEGRRFIDFAAGIAVLNTGHRHPKLLDA 68 Query: 90 IRKQSESLMHSME--VNSSKRTELAAKISEIMPDGLRGDCITFFTQGGSDALEAAVKFAK 147 +R Q + H+ V + ELA +I+ + P F T G++A+E A+K A+ Sbjct: 69 MRAQMDKFTHTAYQIVPYASYVELAERINRLTPGNYPKKTAFFST--GAEAVENAIKIAR 126 Query: 148 RVTGRHQIIAFHGGYHGIWNASNALTTGTA-YRKGFGPFMGGVIHAPYPYAYRFPFDTSH 206 TGR +IAF GG+HG ALT A Y+ GFGPF G V HAPYP A H Sbjct: 127 AHTGRPGVIAFAGGFHGRTMMGMALTGKVAPYKLGFGPFPGDVFHAPYPSAL-------H 179 Query: 207 KSAEQIAGEYVDYLLNTPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGAL 266 + A E V L + A VAA+I+EPVQGEGG+ +F++ LR CD G L Sbjct: 180 GITSEDALEAVKGLFKSD-IEAKRVAAIILEPVQGEGGFYAAPADFMRGLRALCDEHGIL 238 Query: 267 LIVDEVQAGAGRTGKMWAVEHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLAAKIPDGSQP 326 LI DEVQ+G GRTGK++A+EH V PD++T K + G MP++ + R+++ G Sbjct: 239 LIADEVQSGYGRTGKLFAMEHYDVLPDLMTMAKSLAGGMPLSAVNGRAEIMDAPAPGGLG 298 Query: 327 NTFAANSISAAVALTNISILQDPRLDLVNRAHTLGLEAQERIRSFNS--PWVGEVRGRGL 384 T+A N ++ A AL + ++++ + LV R LG + QE ++ S P + EVRG G Sbjct: 299 GTYAGNPLAIASALAVLDVMEEEQ--LVTRGQRLGDKLQEHLKELRSSVPQIAEVRGVGA 356 Query: 385 MIGIELVENRETREPLSREKLGKLMDYVVGHGVLMIPCGRYTNVMRVMPSLTIPRSLMFK 444 M+ +E + T +P E K+ + + +G+L++ CG Y NV+R + LTIP ++M + Sbjct: 357 MVAVEFADP-ATGKP-DAEYTKKVQQHALNNGLLLLTCGSYGNVIRFLFPLTIPDTVMDE 414 Query: 445 GLDIFGAGL 453 L I + Sbjct: 415 ALGILAKAI 423 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 168 bits (426), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 127/374 (33%), Positives = 193/374 (51%), Gaps = 27/374 (7%) Query: 46 RMPIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLM--HSMEV 103 ++P+A+ G+ D DG +ID S G VS +G +PRV+EAIRKQ L H+ Sbjct: 12 KLPMAV-AGEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYAHTSFF 70 Query: 104 NSSKRTELAAKISEIMPDGLRGDCITFFTQGGSDALEAAVKFAKRVT------GRHQIIA 157 ++ ELAA +++ P L +F GGS+A+EAA+K A++ R IIA Sbjct: 71 TTAPAEELAAMLADAAPGSLNH---VYFLSGGSEAVEAALKLARQYYVEVGQPQRRHIIA 127 Query: 158 FHGGYHGIWNASNALTTG-TAYRKG-FGPFMGGVIHAPYPYAYRFPFD--TSHKSAEQIA 213 YHG N AL G A+R+ F P + H YAYR D + + +++A Sbjct: 128 RRQSYHG--NTLGALAIGGNAWRREMFMPMLIEAHHVSPCYAYRNRADGESDVQYVQRLA 185 Query: 214 GEYVDYLLNTPYTAADDVAAVIVEPVQG-EGGYVPPSPEFLQILRKACDRSGALLIVDEV 272 E +L+ AD V A + E V G G VPP ++ + +R CD+ G LLI+DEV Sbjct: 186 DELEQKILSL---GADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLILDEV 242 Query: 273 QAGAGRTGKMWAVEHSGVKPDMLTFGKGIG-GDMPMAGLVMRSDLAAKIPDGS----QPN 327 +G GRTG ++A E GV PD++ KG+G G P+ ++ + + GS + Sbjct: 243 MSGMGRTGYLFACEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSGFFQHGH 302 Query: 328 TFAANSISAAVALTNISILQDPRLDLVNRAHTLGLEAQERIRSFNSPWVGEVRGRGLMIG 387 T+ ++ + A A+ +Q+ RL R L ++ R + VG++RGRGL +G Sbjct: 303 TYIGHATACAAAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGRGLFVG 362 Query: 388 IELVENRETREPLS 401 +ELV R ++ PLS Sbjct: 363 VELVAERSSKLPLS 376 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 142 bits (357), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 121/425 (28%), Positives = 195/425 (45%), Gaps = 39/425 (9%) Query: 48 PIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHS---MEVN 104 P+ + G D +D +G+ + G V+ A +Q + L ++ Sbjct: 29 PLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGYGQESVIRAATEQMQRLPYATGYFHFA 88 Query: 105 SSKRTELAAKISEIMPDGLRGDCITFFTQGGSDALEAAVKFAKRVT------GRHQIIAF 158 S LA K+ +I P L+ +FT GGSDA++AA+++ G+ I+ Sbjct: 89 SEPAILLAKKLVDISPASLQH---VYFTLGGSDAVDAAIRYITHYHHSIGKPGKQHFISL 145 Query: 159 HGGYHGIWNASNALTTGTAYRKGFGPFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGEYVD 218 GYHG + LT + F + + P PY YR + + E I V Sbjct: 146 QRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLPSPYPYR----SELQGDEAIIAASVQ 201 Query: 219 YLLN-TPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAG 277 L + AD+VAA EP+QG GG V P +L+ ++ AC+ L +VDEV G G Sbjct: 202 ALRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAMQLACNELDILFVVDEVITGFG 261 Query: 278 RTGKMWAVEHSGVKPDMLTFGKGI-GGDMPMAGLVMRSDLAAKIPDGS-------QPNTF 329 RTG M+A V+PD++T KG+ G PM ++M + I DG+ T+ Sbjct: 262 RTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDRVYNGIADGAPMSTVVGHGQTY 321 Query: 330 AANSISAAVALTNISILQDPRLDLVNRAHTLGLEAQERIRS-FNSPWVGEVRGRGLMIGI 388 +A+ +SAAVAL + + ++ L++ L +R+ P VG+ R RGL+ + Sbjct: 322 SAHPVSAAVALEVLRLYEEG--GLLDNGRRLEPVFAGGLRALLAHPLVGDARSRGLLGAL 379 Query: 389 ELVENRETREPLSREKLGKLMDYVVG----HGVLMIPCGRYTNVMRVMPSLTIPR---SL 441 ELV +++T+ + E KL D + HG++ G N++ P+L + +L Sbjct: 380 ELVADKQTKARFAPEL--KLHDRIFQAAYRHGLVFRAFG--DNILGFAPALCYTQEDFAL 435 Query: 442 MFKGL 446 MF L Sbjct: 436 MFARL 440 >gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 140 bits (352), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 115/411 (27%), Positives = 191/411 (46%), Gaps = 63/411 (15%) Query: 46 RMPIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSMEVNS 105 R + + G+ D +G ++D G V+++G + +A+ QS+ L++ + Sbjct: 17 RPELVFTEGHGMWLTDHNGKRYLDYLQGWAVNTLGHAPQCIADALAAQSKKLINP---SP 73 Query: 106 SKRTELAAKISEIMPDGLRGDCITFFTQGGSDALEAAVKFAKRV---------TGRHQII 156 + E + ++++++ D + FF G +A E A+K A++ + R +II Sbjct: 74 AFYNEPSIELAKLLTANSVFDRV-FFANSGGEANEGAIKLARKWGKKNPAADGSARFEII 132 Query: 157 AFHGGYHGIWNASNALTTGTAYRKGFGPFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGEY 216 F +HG A+ + + + F P + G +P A +S + + GE+ Sbjct: 133 TFKHSFHGRTLATMSASGKDGWDTMFAPQVPG-----FPKAVL----NDLESVKALIGEH 183 Query: 217 VDYLLNTPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGA 276 AV++EPVQGEGG +P S EF+Q LR LLIVDEVQ+G Sbjct: 184 T--------------VAVMLEPVQGEGGVIPASKEFMQGLRSLTKEKNLLLIVDEVQSGM 229 Query: 277 GRTGKMWAVEHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLAAKIPDGSQPNTFAANSISA 336 GRTG+++A +HSG++PD++T KGIGG +P+A L+ R ++A G Q T+ N + Sbjct: 230 GRTGQLFAYQHSGIEPDIMTLAKGIGGGVPLAALLAREEIAC-FEAGEQGGTYNGNPLMT 288 Query: 337 AVALTNISILQDPRLDLVNRAHTLGLEAQERIRSFNSPWVGEVRGRGLM--------IGI 388 AV + I L P R L + S + GE RG GL+ IG Sbjct: 289 AVGVAVIKELLKPGFMESVRERGQYLRQRSLEISEKYGFEGE-RGEGLLRALQLGRDIGP 347 Query: 389 ELVENRETREPLSREKLGKLMDYVVGHGVLMIPCGRYTNVMRVMPSLTIPR 439 ++VE EP+ L++ R N++R MP+L + + Sbjct: 348 QIVEAARNLEPVG----------------LLLNSPR-PNLLRFMPALNVTK 381 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,530,915 Number of extensions: 68929 Number of successful extensions: 252 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 225 Number of HSP's successfully gapped: 10 Length of query: 456 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 368 Effective length of database: 1,203,190 Effective search space: 442773920 Effective search space used: 442773920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)