BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0025.p (480 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 964 0.0 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 528 e-151 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 362 e-101 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 320 2e-88 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 291 1e-79 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 964 bits (2493), Expect = 0.0, Method: Compositional matrix adjust. Identities = 477/480 (99%), Positives = 477/480 (99%) Query: 1 MYPDLQLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQ 60 MYPDLQLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQ Sbjct: 1 MYPDLQLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQ 60 Query: 61 VSPLERSDILRRAATLARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRL 120 VSPLERSDILRRAA LARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRL Sbjct: 61 VSPLERSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRL 120 Query: 121 YGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 YGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP Sbjct: 121 YGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 Query: 181 AAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAA 240 AAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAA Sbjct: 181 AAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAA 240 Query: 241 LHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFV 300 LHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFV Sbjct: 241 LHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFV 300 Query: 301 TAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSERVTGGARLGQRGH 360 TAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSE VTGGARLGQRGH Sbjct: 301 TAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQRGH 360 Query: 361 FYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQPEEAIAMANALPLGLASYVFTQSLDTA 420 FYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQ EEAIAMANALPLGLASYVFTQSLDTA Sbjct: 361 FYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDTA 420 Query: 421 HRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMISQITHVPA 480 HRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMISQITHVPA Sbjct: 421 HRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMISQITHVPA 480 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 528 bits (1359), Expect = e-151, Method: Compositional matrix adjust. Identities = 246/475 (51%), Positives = 347/475 (73%) Query: 1 MYPDLQLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQ 60 MY D+QL+I+G + R +V +PAT +V+G++ A+RAD++ A++AA + F+ WR Sbjct: 1 MYKDVQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRN 60 Query: 61 VSPLERSDILRRAATLARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRL 120 +S ERS I+RRAA L RERA+ +A +TM+ GKPLA+A E + AA+ +DW AEE RR Sbjct: 61 MSAFERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRT 120 Query: 121 YGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 YGR+V AR P Q+V++EPVG AA +PWNFP Q ++K++AA+AAGC++++K P E+P Sbjct: 121 YGRLVPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETP 180 Query: 181 AAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAA 240 A+ + + + DAGVPAGV N+V GV +EIS++LI P+++ ISFTGS PVG +LAA A Sbjct: 181 ASPAELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAG 240 Query: 241 LHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFV 300 HMKR +MELGGHAP +VFDDA+V+ AA++LA K RNAGQVCVSP+RF VQKG++ FV Sbjct: 241 QHMKRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFV 300 Query: 301 TAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSERVTGGARLGQRGH 360 F Q+KVG G E G+ MGP+ + RR A++ LIDDA++QG++ TGG R+G +G+ Sbjct: 301 DKFVTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGY 360 Query: 361 FYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQPEEAIAMANALPLGLASYVFTQSLDTA 420 F+ PT++T +P AR M EEPFGP+A + F +E I AN LP GLA+Y F +S T Sbjct: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 Query: 421 HRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMISQI 475 H ++ +++GM+ INH G+A PELPFGGI +SG+GSEGG E E+++ TK+++Q+ Sbjct: 421 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVTQL 475 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 362 bits (930), Expect = e-101, Method: Compositional matrix adjust. Identities = 189/468 (40%), Positives = 282/468 (60%) Query: 6 QLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQVSPLE 65 Q +++G++ + V +PA G++LG +P A+ AI+AA+ A+ W++ + E Sbjct: 13 QAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKE 72 Query: 66 RSDILRRAATLARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRLYGRVV 125 RS ILRR L +A +T + GKPLA+A EI AA ++W AEEG+R YG + Sbjct: 73 RSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTI 132 Query: 126 GARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESPAAIVL 185 + P R +V++E +GVCAAI+PWNFP +K A+AAGC +VLK +P + + Sbjct: 133 PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALA 192 Query: 186 MAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAALHMKR 245 +A L AG+PAGVF++V+G I + +P+VR ISFTGS VG L Q+A +K+ Sbjct: 193 LAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKK 252 Query: 246 MSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFVTAFAE 305 +S+ELGG+AP +VFDDA+++ A K RNAGQ CV +R YVQ G+Y F E Sbjct: 253 LSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVE 312 Query: 306 ALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSERVTGGARLGQRGHFYAPT 365 A+ + KVG G E GV GPLI+ + + V+ + DA+A+G+ + GG R F+ PT Sbjct: 313 AVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPT 372 Query: 366 LITGVPPSARVMQEEPFGPIAAVTPFSQPEEAIAMANALPLGLASYVFTQSLDTAHRVSR 425 ++ V P+ +V +EE FGP+A + F EE +A+AN GLASY +++ + RV+ Sbjct: 373 VLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAE 432 Query: 426 DLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMIS 473 L++GMV IN +++ PFGG+ +SG G EG G + +LV K + Sbjct: 433 GLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 480 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 320 bits (819), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 194/482 (40%), Positives = 282/482 (58%), Gaps = 10/482 (2%) Query: 1 MYPDLQLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQ 60 + P QL I + G R +V DPATG V +P AD A++AA AF WR Sbjct: 11 LMPGAQL-IGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFAAWRA 69 Query: 61 VSPLERSDILRRAATLARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRL 120 +R+ I++R L + I+ + GKPLA+A E+ AA +++W EE R Sbjct: 70 TPAKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRA 129 Query: 121 YGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 G ++ A R + ++EPVGV AAI+PWNFP +K+A A+AAGCTVV K ++P Sbjct: 130 NGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTP 189 Query: 181 AAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPL----VRNISFTGSVPVGSKLA 236 + + +L +AGVP GV NIV+ S E + +++ L VR ISFTGS VG LA Sbjct: 190 LTSLALVRLAQEAGVPVGVINIVTA-SRERTPEVVDVWLADGRVRKISFTGSTAVGKHLA 248 Query: 237 AQAALHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIY 296 +A +K++S+ELGG+AP +VFDDA+V+ A + K RN GQ CVSP+R YVQ+ +Y Sbjct: 249 RHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVY 308 Query: 297 ARFVTAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSERVTGGARLG 356 FV + +KVGP +P Q+GP+I+ R + + DA+A+G+ +TGG RL Sbjct: 309 DAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKRLQ 368 Query: 357 QRG----HFYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQPEEAIAMANALPLGLASYV 412 G ++YAPT++ V + + EE FGP+A +T F+ +E IA ANA P GLA+Y Sbjct: 369 GPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLAAYF 428 Query: 413 FTQSLDTAHRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMI 472 ++ + HRV+ L++G+V +N +A PFGG+ ESG+G EG V G + +L TK + Sbjct: 429 YSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDYLHTKYV 488 Query: 473 SQ 474 Q Sbjct: 489 CQ 490 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 291 bits (744), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 184/476 (38%), Positives = 262/476 (55%), Gaps = 20/476 (4%) Query: 6 QLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQVSPLE 65 YIDG++ +G V PAT + + + AD+++A+QAA RA W SPLE Sbjct: 17 HFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLE 76 Query: 66 RSDILRRAATLARERAQAMAPAITMDNGKPLADA-VAEIINAAEHLDWHAEEGRRLYGRV 124 R+ +L R L ER++ A A+T++ G P++ A A + AAEHL R Sbjct: 77 RAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLR---------VARD 127 Query: 125 VGARHPEVR----QLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 A +P +R +VREP+GVCA I+PWN+P Q KV A+AAGCTVVLK SP Sbjct: 128 NLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSP 187 Query: 181 AAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAA 240 + +L A++ H+AGVPAGVFN+V+G +E+ L P V IS TGS G +A AA Sbjct: 188 LSALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAA 247 Query: 241 LHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFV 300 + +KR++ ELGG +P LV DA++E A RN GQ C +P+R V + AR Sbjct: 248 VTVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVH 307 Query: 301 TAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSERVTGGA----RLG 356 L Q+KVG ++ GPL + +F VQ +I+ L G+ + GG L Sbjct: 308 ELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLA 367 Query: 357 QRGHFYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQPEEAIAMANALPLGLASYVFTQS 416 Q G++ PT+ + V + QEE FGP+ A+ P+ EEAIA+AN GL ++V + Sbjct: 368 Q-GYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRD 426 Query: 417 LDTAHRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMI 472 D V+ +Q+G V++N+ P+ PFGG +SG G E GVEG E ++ K I Sbjct: 427 KDQVRAVAARIQSGQVHLNYPAW-DPQAPFGGYKQSGNGREYGVEGMEEYMEVKAI 481 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,481,040 Number of extensions: 63099 Number of successful extensions: 288 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 230 Number of HSP's successfully gapped: 21 Length of query: 480 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 391 Effective length of database: 1,198,455 Effective search space: 468595905 Effective search space used: 468595905 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)