BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0024.p (544 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ... 1105 0.0 gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hy... 108 1e-24 gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hy... 81 2e-16 gi|300313270|ref|YP_003777362.1| 4-hydroxybenzoate 3-monooxygena... 56 6e-09 >gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate hydroxylase [Herbaspirillum seropedicae SmR1] Length = 544 Score = 1105 bits (2858), Expect = 0.0, Method: Compositional matrix adjust. Identities = 541/544 (99%), Positives = 542/544 (99%) Query: 1 MSAVGQQQAVPAQVMQAQVLIVGAGPVGVTLGNLLGTYGVSTLLIERNLDVLDYPRAVGI 60 MSAVGQQ AVPAQVMQAQVLIVGAGPVGVTLGNLLGTYG+STLLIERNLDVLDYPRAVGI Sbjct: 1 MSAVGQQPAVPAQVMQAQVLIVGAGPVGVTLGNLLGTYGISTLLIERNLDVLDYPRAVGI 60 Query: 61 DDEALRTFQSAGMADAIVRDVIQNVPMRMYTADRRCFAEIQPTTREFGWYRRNLFSQPLG 120 DDEALRTFQSAGMADAIVRDVIQNVPMRMYTADRRCFAEIQPTTREFGWYRRNLFSQPLG Sbjct: 61 DDEALRTFQSAGMADAIVRDVIQNVPMRMYTADRRCFAEIQPTTREFGWYRRNLFSQPLG 120 Query: 121 EATLRLGLRRFEHVSLRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGGRST 180 EATLRLGLRRFEHVSLRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGGRST Sbjct: 121 EATLRLGLRRFEHVSLRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGGRST 180 Query: 181 VREKILKLPYEGKTHPAKWVVIECDGDPLDAPYTALHCEPQRPYVCLALPYGVRRWEFML 240 VREKILKLPYEGKTHPAKWVVIECDGDPLDAPYTALHCEPQRPYVCLALPYGVRRWEFML Sbjct: 181 VREKILKLPYEGKTHPAKWVVIECDGDPLDAPYTALHCEPQRPYVCLALPYGVRRWEFML 240 Query: 241 FPGEDGEQMLAPEKVRELLREHVRDVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAH 300 FPGEDGEQMLAPEKVRELLREHVRDVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAH Sbjct: 241 FPGEDGEQMLAPEKVRELLREHVRDVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAH 300 Query: 301 ITPPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEERHAHVKAMIDLADLFGA 360 ITPPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEERHAHVKAMIDLADLFGA Sbjct: 301 ITPPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEERHAHVKAMIDLADLFGA 360 Query: 361 VLSQKNRLLAWCRDKFFLAIKDIPRVRDYVLQMKFKPMPSFSSQAVMQDDARNRSDLVGR 420 VLSQKNRLLAWCRDKFFLAIKDIPRVRDYVLQMKFKPMPSFSSQAVMQDD RNRSDLVGR Sbjct: 361 VLSQKNRLLAWCRDKFFLAIKDIPRVRDYVLQMKFKPMPSFSSQAVMQDDVRNRSDLVGR 420 Query: 421 MFLQSLVENGAGEVMRLDDLCGGRFVLLSWRCDVLAAAEPGLRSRMQAFGCDYLQGVRSR 480 MFLQSLVENGAGEVMRLDDLCGGRFVLLSWRCDVLAAAEPGLRSRMQAFGCDYLQGVRSR Sbjct: 421 MFLQSLVENGAGEVMRLDDLCGGRFVLLSWRCDVLAAAEPGLRSRMQAFGCDYLQGVRSR 480 Query: 481 SGPRGQVLAGAGVVIQDYENKLNAWFKEKGVDWVLLRPDRFIAAAGRAGDAPEVVARYLD 540 SGPRGQVLAGAGVVIQDYENKLNAWFKEKGVDWVLLRPDRFIAAAGRAGDAPEVVARYLD Sbjct: 481 SGPRGQVLAGAGVVIQDYENKLNAWFKEKGVDWVLLRPDRFIAAAGRAGDAPEVVARYLD 540 Query: 541 QVAA 544 QVAA Sbjct: 541 QVAA 544 >gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 589 Score = 108 bits (269), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 98/350 (28%), Positives = 163/350 (46%), Gaps = 22/350 (6%) Query: 7 QQAVPAQVMQAQVLIVGAGPVGVTLGNLLGTYGVSTLLIERNLDVLDYPRAVGIDDEALR 66 +Q P V+I+GAGPVG+ L G++ +L++ + + RA+ L Sbjct: 21 EQQSPGHAPVHPVVIIGAGPVGLACAIDLARQGIAVVLVDDDDTLSTGSRAICFAKRTLD 80 Query: 67 TFQSAGMADAIVRDVIQNVPMRMYTADRRCFA-EIQPTTREFGWYRRNLFS--QPLGEAT 123 F G +V + +++ D+ + ++ P E G R + Q E Sbjct: 81 IFDRIGCGQRMVDKGVGWNRGKVFLRDQAVYEFDLLP---ESGHARPAFINLQQYYVEGY 137 Query: 124 LRLGLRRFEHVSLRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGGRSTVRE 183 L +++ H+ +R ++ + E +A +D TL+A+YVV DG RS +R Sbjct: 138 LLEQAQQWPHIEIRWKNKVSAIAHHEDH-EALTVDTPQGSYTLKARYVVAADGSRSPLR- 195 Query: 184 KILKLPYEGKTHPAKWVVIECDGDPLDAPYTALH-------CEPQRPYVCLALPYGVRRW 236 K++ L +G T ++++ + +DAP+ A PQ+ + P V R Sbjct: 196 KMMGLESQGHTFRDRFLIADVK---MDAPFPAERWFWFDPPFHPQQSVLLHRQPDDVWRI 252 Query: 237 EFMLFPGEDGEQML--APEKVRELLREHVRDVDALNIIRARVYTHNSRVAGSLVCGRICL 294 +F L G D + +L PE+V +R + + + ++ VYT + GR+ Sbjct: 253 DFQL--GWDADPVLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRMERFRHGRVLF 310 Query: 295 AGDAAHITPPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEER 344 GDAAH P+ +G N+G++DA NLAWKLA VLQG+ LL SY ER Sbjct: 311 VGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASER 360 >gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 547 Score = 80.9 bits (198), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 90/363 (24%), Positives = 148/363 (40%), Gaps = 49/363 (13%) Query: 6 QQQAVPAQVMQAQVLIVGAGPVGVTLGNLLGTYGVSTLLIERNLDVLDYPRAVGIDDEAL 65 Q++A P++ V+++GAGP+G+T G+ ++++ N V RAV L Sbjct: 18 QREARPSR---HPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTVSIGSRAVCYAKRPL 74 Query: 66 RTFQSAGMADAIVRDVIQNVPMRMYTADRRCF--------AEIQPTTREFGWYRRNLFSQ 117 + G+A +V + +++ +R + A P Y Q Sbjct: 75 EIWDRLGVAPRMVEKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPAMINLQQY---YLEQ 131 Query: 118 PLGEATLRLGLRRFEHVSLRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGG 177 L EA L + LR +++ L QD+ + G + A +V+ CDG Sbjct: 132 YLVEACQAQPL-----IDLRWKHKMISLHQDDTHATLDVETPDGI-FRMEADWVIACDGA 185 Query: 178 RSTVREKILKLPYEGKTHPAKW----VVIECDGDP-----LDAPY-----TALH--CEPQ 221 S R K++ + G+ ++ V+++ D D P+ LH C+ Sbjct: 186 NSDTR-KMVGAEFSGQFFQDRFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQCD-- 242 Query: 222 RPYVCLALPYGVRRWEFMLFPGEDGEQMLAPEKVRELLREHVRDVDALNIIRARVYTHNS 281 V R +F L D E+ PEKV ++ + + VY Sbjct: 243 ----------NVWRIDFQLGWEADPEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFAC 292 Query: 282 RVAGSLVCGRICLAGDAAHITPPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYH 341 R + R+ AGDAAH P+ +G N G++D NL WKL V+ G+ P + SYH Sbjct: 293 RRIDKMRYQRVLFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAPPSFIDSYH 352 Query: 342 EER 344 EER Sbjct: 353 EER 355 >gi|300313270|ref|YP_003777362.1| 4-hydroxybenzoate 3-monooxygenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 56.2 bits (134), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 73/319 (22%), Positives = 139/319 (43%), Gaps = 22/319 (6%) Query: 15 MQAQVLIVGAGPVGVTLGNLLGTYGVSTLLIERNL--DVLDYPRAVGIDDEALRTFQSAG 72 M+ QV I+GAGP G+ L +LL G+ ++++E ++ RA ++ + G Sbjct: 1 MRTQVAIIGAGPAGLLLSHLLHLKGIESVVLETRSREEIESTIRAGVLEQGTMDILTETG 60 Query: 73 MADAIVRDVIQNVPMRMYTADRRCFAEI-QPTTREFGWYRRNLFSQPLGEATLRLGLRRF 131 + + + R+ + + + RR ++ + T + Y ++ + L A L + Sbjct: 61 VGERMKREGALHHGIELAFGGRRHRIDLTELTGQAITVYAQHEVIKDLVAARLAAQGQLL 120 Query: 132 EHVSLRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGGRSTVREKI---LKL 188 VS G + + D+ V+ G Q TL A ++ GCDG R I + Sbjct: 121 FSVS---GTSIEGVETDKPRVR---FMHEGEQHTLEADFIAGCDGFHGVSRPAIPDSKRQ 174 Query: 189 PYEGKTHPAKWVVIECDGDPLDAPYTALHCEPQRPYVCLAL-PYGVRRWEFMLFPGEDGE 247 Y+ + +P W + + P + ++ + +R +V ++ V+R F P +D Sbjct: 175 DYQ-RIYPFGWFGVLVEAPP--SSDELIYAQHERGFVLVSTRSPTVQRLYFQCDP-KDSV 230 Query: 248 QMLAPEKVRELLREHVRDVDALNIIRARVYTHN-----SRVAGSLVCGRICLAGDAAHIT 302 + +++ + + D + +++ S V+ + GR+ LAGDAAHI Sbjct: 231 DNWSDDRIWNEFHTRLENGDGWRLKEGKIFQKGIIGMRSFVSTPMQHGRLFLAGDAAHIV 290 Query: 303 PPWIGQGLNAGLRDAFNLA 321 PP +GLN + D LA Sbjct: 291 PPTGAKGLNLAVGDVKRLA 309 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,795,167 Number of extensions: 80333 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 254 Number of HSP's successfully gapped: 4 Length of query: 544 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 454 Effective length of database: 1,193,720 Effective search space: 541948880 Effective search space used: 541948880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)