BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0022.p (289 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil... 592 e-170 gi|300311290|ref|YP_003775382.1| hydrolase or acyltransferase [H... 108 6e-25 gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum serop... 100 3e-22 gi|300310656|ref|YP_003774748.1| alpha/beta hydrolase superfamil... 76 3e-15 >gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 289 Score = 592 bits (1525), Expect = e-170, Method: Compositional matrix adjust. Identities = 286/289 (98%), Positives = 287/289 (99%) Query: 1 MQRRDYLLTLSALNFHITEWGDPQGFPLLLLHGIRGYGETFGDLAQRLPPGYRCIALDQR 60 MQRRDYLLTLSALNFHITEWGDPQGFPLLLLHGIRGYGETFGDLAQRLPPGYRCIALDQR Sbjct: 1 MQRRDYLLTLSALNFHITEWGDPQGFPLLLLHGIRGYGETFGDLAQRLPPGYRCIALDQR 60 Query: 61 GRGQSDWDAGANYYTDAYVADMEALVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRL 120 GRGQSDWDAGANYYTDAYVAD+EALVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRL Sbjct: 61 GRGQSDWDAGANYYTDAYVADVEALVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRL 120 Query: 121 IIEDAGPGAFENSPGATRIRRELATTPSHFASWDEASAFMRALRPSVTEEARQQRLHNML 180 IIEDAGPGAFENSPGATRIRRELATTPSHFASWDEAS FMRALRPSVTEEARQQRLHNML Sbjct: 121 IIEDAGPGAFENSPGATRIRRELATTPSHFASWDEASTFMRALRPSVTEEARQQRLHNML 180 Query: 181 KPAVQGGYTWRYDHVGISSTRLSPDLSRVVDLRPHIMALMCPTLVVRGERSDYLSAEMAA 240 KPA QGGYTWRYDHVGISSTRLSPDLSRVVDLRPHIMALMCPTLVVRGERSDYLSAEMAA Sbjct: 181 KPAAQGGYTWRYDHVGISSTRLSPDLSRVVDLRPHIMALMCPTLVVRGERSDYLSAEMAA 240 Query: 241 EMRQLNARILTSTIADAGHYIHDDQPEAFAEVVRQFLAQEQHETVEENP 289 EMRQLNARILTSTIADAGHYIHDDQPEAFAEVVRQFLAQEQHETVEENP Sbjct: 241 EMRQLNARILTSTIADAGHYIHDDQPEAFAEVVRQFLAQEQHETVEENP 289 >gi|300311290|ref|YP_003775382.1| hydrolase or acyltransferase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 108 bits (269), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 85/270 (31%), Positives = 135/270 (50%), Gaps = 18/270 (6%) Query: 19 EWGDPQGFPLLL-LHGIRGYGETFGDLAQRLPPGYRCIALDQRGRGQSDWDAGANYYT-D 76 EWGD + +L+ +HG+ + F +A+ L YR I D GRG+S A +YT Sbjct: 24 EWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRVICPDVVGRGRSGRLANPQFYTVP 83 Query: 77 AYVADMEALVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRLIIEDAGPGAFENSPGA 136 YV+DM L+ARL+ + D G SMGG+ + A+ +RRL++ D GP N Sbjct: 84 QYVSDMVTLLARLDAQIVDWFGTSMGGLIGMALASLPGNPIRRLVLNDIGPSI--NGAAL 141 Query: 137 TRIRRELATTPSHFASWDEASAFMRALRPSV---TEEARQQRLHNMLKPAVQGGYTWRYD 193 RI + F S++EA+ ++R + S ++E + ++L+ QG + YD Sbjct: 142 ARIGDYIGQD-VRFDSFEEAAQYIRTISASFGQHSDEEWHKLAKDVLRQNEQGQWVRHYD 200 Query: 194 -HVGISSTRLSPDLSRVVD--LRPHIMALMCPTLVVRGERSDYLSAEMAAEMRQLNARIL 250 + + + +P+ + + L A+ CPTL+VRG +SD L E+A EM + + Sbjct: 201 LALALPFKQTTPEAASAAEQMLWAAYDAIRCPTLLVRGAQSDLLLPEVAQEMTRRGPKAK 260 Query: 251 TSTIADAGH---YIHDDQPEAFAEVVRQFL 277 + D GH ++H Q EV RQFL Sbjct: 261 LVELPDVGHAPTFMHAPQ----IEVARQFL 286 >gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum seropedicae SmR1] Length = 300 Score = 99.8 bits (247), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 80/298 (26%), Positives = 125/298 (41%), Gaps = 32/298 (10%) Query: 4 RDYLLTLSALNFHITEWGDPQGFPLLLLHGIRGYGETFGDLAQRLPPGYRCIALDQRGRG 63 R LT+ L +H+ WGDP+ + +LHG +F + L + IA D RG G Sbjct: 11 RSEFLTIRGLRYHVRHWGDPEAPKIFMLHGWMDMSASFQFMVDALQGQWHVIAPDWRGFG 70 Query: 64 QSDWDAGANYYTDAYVADMEALVARL-NLRQFDLLGHSMGGINAIVYAARHPAKVRRLII 122 ++ +Y+ Y+AD++A++ +LLGHSMGG +YA P +++RLI Sbjct: 71 LTERSQADSYWFPDYLADLDAIIDHYAGSEAINLLGHSMGGNVVTLYAGVRPQRIKRLI- 129 Query: 123 EDAGPGAFENSPGATRIRRELATTPSHFASWDEASAFMRALRPSVTEEARQQRLH----- 177 N G + P FA W + LR T E QRL Sbjct: 130 ---------NLEGLGLPATQPEQAPGRFAQWMDEVRNRPVLRGYATLEEVAQRLQRNNAR 180 Query: 178 -NMLKPAVQGGYTWRYDHVGI-------SSTRLSPDLSRVVDLRPHIMALMCPTLVVRGE 229 + + A + + D G+ + ++SP L R ++ + P L + Sbjct: 181 LSDERAAFLAQHWAQQDDSGLWQILGDPAHKQVSPILYRADEMMACWRRITAPVLWIEAI 240 Query: 230 RSDYLSAEMAAEMRQ--------LNARILTSTIADAGHYIHDDQPEAFAEVVRQFLAQ 279 SD A +++ R+ IADAGH +H DQPE A+++ FL Q Sbjct: 241 DSDIWRFFGPASLKREEIDRRIACIPRVQVEMIADAGHMLHHDQPEQLAQLIEAFLQQ 298 >gi|300310656|ref|YP_003774748.1| alpha/beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 277 Score = 75.9 bits (185), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 75/261 (28%), Positives = 110/261 (42%), Gaps = 37/261 (14%) Query: 28 LLLLHGIRGYGETFGDLAQRLPPGYRCIALDQRGRGQSDWDAGANYYTDAYVADMEALVA 87 +++L GI T+G + +R + LD RGRG S +Y DA AD+ AL Sbjct: 39 VIILPGITSPAVTWGFVGERFGQQFDTYVLDVRGRGLSSASDTLDYSVDAQAADVIALAQ 98 Query: 88 RLNLRQFDLLGHSMGGINAIVYAARHPAKVRRLIIED---AGPGAFENSPGATRIRRELA 144 L L ++ ++GHSMG A + P + RL++ D +GPG R + Sbjct: 99 ALGLSRYAIVGHSMGARIGARAARQTPQGLTRLVMVDPPVSGPGR----------RAYPS 148 Query: 145 TTPSHFASWDEASAF-----MRALRPSVTEEARQQR---LHNMLKPAVQGGYTWRYDHVG 196 P + S A A MRA + TEE Q R LH + AV + H G Sbjct: 149 KLPWYVDSMRLARAGCSAEQMRAFCATWTEEQLQLRAEWLHTCDERAVIASF--EEFHTG 206 Query: 197 ISSTRLSPDLSRVVDLRPHIMALMCPTLVVRGERSDYLSAEMAAEMRQLNARILTSTIAD 256 D + AL P ++++ R D + E AE+R L +++ S + + Sbjct: 207 --------------DFHADLPALRLPVMLMQASRGDVILPEDVAEIRSLLPQVVVSRVEE 252 Query: 257 AGHYIHDDQPEAFAEVVRQFL 277 AGH I D F R FL Sbjct: 253 AGHMIPWDNEAGFYAAFRDFL 273 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 973,020 Number of extensions: 42377 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 193 Number of HSP's successfully gapped: 8 Length of query: 289 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 205 Effective length of database: 1,222,130 Effective search space: 250536650 Effective search space used: 250536650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)