BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0018.p (264 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum s... 523 e-150 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 116 2e-27 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 108 4e-25 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 107 7e-25 gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 107 7e-25 >gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 523 bits (1348), Expect = e-150, Method: Compositional matrix adjust. Identities = 264/264 (100%), Positives = 264/264 (100%) Query: 1 MELKLQGKAAVVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASALSQSTGTEVI 60 MELKLQGKAAVVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASALSQSTGTEVI Sbjct: 1 MELKLQGKAAVVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASALSQSTGTEVI 60 Query: 61 AVAADVSNTEQVEAAVAQAAQHFGRIDILVNGAAHPGGLVRSEIQHASPEGLLEDINIKV 120 AVAADVSNTEQVEAAVAQAAQHFGRIDILVNGAAHPGGLVRSEIQHASPEGLLEDINIKV Sbjct: 61 AVAADVSNTEQVEAAVAQAAQHFGRIDILVNGAAHPGGLVRSEIQHASPEGLLEDINIKV 120 Query: 121 VGYMRFAKAVAPHMRAGGYGRIINIGGLTGRGSKQLSGMRNVAICHLTKTLSDQLGPDGI 180 VGYMRFAKAVAPHMRAGGYGRIINIGGLTGRGSKQLSGMRNVAICHLTKTLSDQLGPDGI Sbjct: 121 VGYMRFAKAVAPHMRAGGYGRIINIGGLTGRGSKQLSGMRNVAICHLTKTLSDQLGPDGI 180 Query: 181 TVNVIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAKATPIRRVLQVEEIADVVLFLA 240 TVNVIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAKATPIRRVLQVEEIADVVLFLA Sbjct: 181 TVNVIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAKATPIRRVLQVEEIADVVLFLA 240 Query: 241 SPRAAAITGESIAVDGGITRGIYI 264 SPRAAAITGESIAVDGGITRGIYI Sbjct: 241 SPRAAAITGESIAVDGGITRGIYI 264 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 116 bits (290), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 82/258 (31%), Positives = 130/258 (50%), Gaps = 19/258 (7%) Query: 8 KAAVVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASALSQSTGTEVIAVAADVS 67 + A+VTGG++GIG ++++ LA +G V + + + A AL + G + D+ Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALV-AAGHRAAPLQMDLG 67 Query: 68 NTEQVEAAVAQAAQHFGRIDILVNGAAHPGGLVRSEIQHASP-EGLLEDINIKVVGYMRF 126 + E + AA A + +GR D+LVN A G+ R+ P E L+ +N+ V G + Sbjct: 68 SPESIAAAFAHIEKDYGRCDVLVNNA----GVARTYSFLDYPLENWLQTMNVNVTGVLLA 123 Query: 127 AKAVAPHMRAGGYGRIINIGGLTG---RGSKQLSGMRNVAICHLTKTLSDQLGPDGITVN 183 + A M +GRI+NI ++G + G A+ LT+ ++ +L GITVN Sbjct: 124 GQHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVN 183 Query: 184 VIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAKATPIRRVLQVEEIADVVLFLASPR 243 + PG V+TP Q + EQ ++Y + P+RR +EIA V +LA+ Sbjct: 184 SVAPGPVDTP----------MTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEE 233 Query: 244 AAAITGESIAVDGGITRG 261 +A ITG I VDGG G Sbjct: 234 SAYITGHVIPVDGGFVAG 251 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 108 bits (270), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 90/259 (34%), Positives = 126/259 (48%), Gaps = 21/259 (8%) Query: 4 KLQGKAAVVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASALSQSTGTEVIAVA 63 +L GK A++TGG GIGRAVA A AREG R+AIV + + Q+ + + Sbjct: 122 RLAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLP 181 Query: 64 ADVSNTEQVEAAVAQAAQHFGRIDILVNGAA--HPGGLVRSEIQHASPEGLLEDINIKVV 121 DV + E +AV +GR+D+LVN AA +P + EI A E NI + Sbjct: 182 GDVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSIE-EISQAQLEHTFR-TNIFSM 239 Query: 122 GYMRFAKAVAPHMRAGGYGRIINIGGLTG-RGSKQLSGMRNV--AICHLTKTLSDQLGPD 178 YM K P + G RIIN +T RGSK L AI T++LS QL Sbjct: 240 FYM--VKEAMPFLTRG--ARIINTTSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLASA 295 Query: 179 GITVNVIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAKATPIRRVLQVEEIADVVLF 238 GI VN + PG + TP I + E ++ K P+ R Q +E+A +F Sbjct: 296 GIHVNAVAPGPIWTPLIPASFSAEEV----------ASFGKNVPLGRPGQPDEVAPAYVF 345 Query: 239 LASPRAAAITGESIAVDGG 257 LA+ A+ +TG+ + +GG Sbjct: 346 LATEGASYMTGQVLHPNGG 364 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 107 bits (268), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 90/261 (34%), Positives = 134/261 (51%), Gaps = 19/261 (7%) Query: 3 LKLQGKAAVVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASALSQSTGTEVIAV 62 L L GK ++G S GIG A A A A G + + S K E +A ++ G++ I + Sbjct: 2 LDLAGKVVFISGASTGIGAAAARAFAARGSHLVVHYNSSEKEAEAVAAEVRAAGSKSITI 61 Query: 63 AADVSNTEQVEAAVAQAAQHFGRIDILVNGAAHPGGLVRSEIQHASPEGLLED-INIKVV 121 ADV +T + AAVA+A GRID+L+N A G LV+ + L +D INI Sbjct: 62 GADVRDTSAINAAVAKAVAQMGRIDVLINNA---GALVKRAPLETVTDELFDDIININAR 118 Query: 122 GYMRFAKAVAPHMRAGGYGRIINIGGLTGRGSKQLSGMRNVA----ICHLTKTLSDQLGP 177 + F +AV P MRA G G IIN+ + R + A + LT+ ++ +L Sbjct: 119 SVVAFTRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLA 178 Query: 178 DGITVNVIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAKATPIRRVLQVEEIADVVL 237 D I VN + PGV+ TP E + TPEQ+E + K P+ R+ + +E + L Sbjct: 179 DKIRVNAVAPGVIMTP-FQERFT--------TPEQLE-GFRKTIPMGRIGEPDECSGAFL 228 Query: 238 FLASPRAAA-ITGESIAVDGG 257 +LAS + + +TG+ I V+GG Sbjct: 229 YLASEQMSGYVTGQIIDVNGG 249 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 107 bits (268), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 78/262 (29%), Positives = 130/262 (49%), Gaps = 9/262 (3%) Query: 1 MELKLQGKAAVVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASALSQSTGTEVI 60 M+L L+ + +VT G+ GIG A+ A G V I S+ L ASA ++ V Sbjct: 1 MDLNLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVSEDFL---ASARARFGHAPVS 57 Query: 61 AVAADVSNTEQVEAAVAQAAQHF-GRIDILVNGAAHPGGLVRSEIQHASPEGLLEDINIK 119 DVS+ +V+A A AQ + GR+D+L+N A G S ++ + + + + Sbjct: 58 YSRTDVSSEREVDAMFADLAQRWSGRLDVLINNAGIAGP--TSPVEEVALSDWDQTLAVN 115 Query: 120 VVGYMRFAKAVAPHMRAGGYGRIINIGGLTGRGSKQLS---GMRNVAICHLTKTLSDQLG 176 + G + P ++ G G I+NI + GR L + LT+T + +LG Sbjct: 116 LTGPFLCTRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELG 175 Query: 177 PDGITVNVIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAKATPIRRVLQVEEIADVV 236 P I VN + PG+VE + +A G+ E++ Q +R+++ E+IA+ V Sbjct: 176 PSHIRVNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQV 235 Query: 237 LFLASPRAAAITGESIAVDGGI 258 +FL SP A+I+G+S++V G + Sbjct: 236 IFLCSPAGASISGQSLSVCGNV 257 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 812,632 Number of extensions: 33702 Number of successful extensions: 388 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 286 Number of HSP's successfully gapped: 49 Length of query: 264 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 181 Effective length of database: 1,226,865 Effective search space: 222062565 Effective search space used: 222062565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)