BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0013.p (518 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 853 0.0 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 290 2e-79 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 280 2e-76 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 278 7e-76 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 274 1e-74 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 853 bits (2203), Expect = 0.0, Method: Compositional matrix adjust. Identities = 422/423 (99%), Positives = 423/423 (100%) Query: 3 MSLHLQHFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAW 62 MSLHLQHFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAW Sbjct: 1 MSLHLQHFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAW 60 Query: 63 SKLSGLQRGQLLHKLAALVERDTELLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWA 122 SKLSGLQRGQLLHKLAALVERDT+LLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWA Sbjct: 61 SKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWA 120 Query: 123 NQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIK 182 NQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIK Sbjct: 121 NQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIK 180 Query: 183 PAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRA 242 PAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRA Sbjct: 181 PAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRA 240 Query: 243 IQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRS 302 IQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRS Sbjct: 241 IQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRS 300 Query: 303 LAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVS 362 LAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVS Sbjct: 301 LAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVS 360 Query: 363 RPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWT 422 RPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWT Sbjct: 361 RPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWT 420 Query: 423 TNL 425 TNL Sbjct: 421 TNL 423 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 290 bits (743), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 174/421 (41%), Positives = 242/421 (57%), Gaps = 12/421 (2%) Query: 8 QHFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKLSG 67 QHFIA + G+ + +++P + + + + A+G A D+ +AV AA+ EG AW +LS Sbjct: 3 QHFIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEG-AWGRLSA 61 Query: 68 LQRGQLLHKLAALVERDTELLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQMEG 127 +R +LL KLA ++ E LA ++ R G+ PM+ D + + AG A+++ G Sbjct: 62 AERSRLLMKLAYKLQDHQEELAQLEGRDCGK-PMKQARADAAAIVRYFEFYAGAADKLHG 120 Query: 128 RTIP-TAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEE 186 TIP AGY T+REP GV G I+PWN P+ I V LAAG T V+KPAE+ Sbjct: 121 DTIPYQAGYT-----VLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAED 175 Query: 187 TPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRI 246 S L +A LA E GFP+G +N+VTGYG+ G L +HPDV ISFTGSP G+ + R Sbjct: 176 ACLSILRVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRA 235 Query: 247 AGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQR 306 A E VTLELGGKSPQIVF+DA E L + N GQ C+AGSR+LVQR ++ Sbjct: 236 AAEHHTPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQ 295 Query: 307 FAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGG---VSR 363 + L+ + + VG SQ + GP+ +K Q ERV ++ +G L A G Sbjct: 296 VLERLSGLFSQLQVGS-SQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGV 354 Query: 364 PDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTT 423 P G++ P + D +A+EE+FGPV + F+ E AIALAN + YGL A+VWT Sbjct: 355 PTGGFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTR 414 Query: 424 N 424 + Sbjct: 415 D 415 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 280 bits (716), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 177/424 (41%), Positives = 233/424 (54%), Gaps = 14/424 (3%) Query: 1 MSMSLHLQHF-IAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEG 59 ++ + L HF I G+ +R +V P EE A G AADV +AV AA+ + Sbjct: 9 LTRNARLDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIP- 67 Query: 60 GAWSKLSGLQRGQLLHKLAALVERDTELLADMDARAIGRSPMEPRMMDVPNAISHLRAAA 119 AW+ S L+R LL ++ AL+ +EL A +G R VP A HLR A Sbjct: 68 -AWAATSPLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLRVAR 126 Query: 120 GWANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTV 179 + ++ + VREPIGV I PWN P+ + KV LAAGCTV Sbjct: 127 D--------NLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTV 178 Query: 180 VIKPAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEA 239 V+KP+E +P SAL A + EAG P GV N+V G G VG AL HP V IS TGS A Sbjct: 179 VLKPSELSPLSALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRA 238 Query: 240 GRAIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILV 299 G + + A KRV ELGGKSP +V DA E A+ N GQ C+A +R++V Sbjct: 239 GVLVAQAAAVTVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIV 298 Query: 300 QRSLAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAG 359 R+ R + A + VGDP+ GP+A +AQF RV + I+ GL +GA LLAG Sbjct: 299 PRAALARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAG 358 Query: 360 GVSRPD---KGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGL 416 G RPD +G++ +PTIF++ + M IA+EEIFGPV I+ +DT EEAIA+AND+ YGL Sbjct: 359 GPGRPDGLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGL 418 Query: 417 AATV 420 A V Sbjct: 419 GAHV 422 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 278 bits (711), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 174/420 (41%), Positives = 238/420 (56%), Gaps = 19/420 (4%) Query: 9 HFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKLSGL 68 HFIAG T+ ++ ++P + ++ A +QG+AADV AV AA+A L G W L G Sbjct: 35 HFIAGRFTQPK--ADLDDIDPASGKLLAHLSQGSAADVDAAVQAAQAALPG--WQALGGH 90 Query: 69 QRGQLLHKLAALVERDTELLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQMEGR 128 R + L+ LA V+R LLA ++ G+ E R +DVP H AGWA E Sbjct: 91 GRARHLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAGWAQLQESE 150 Query: 129 TIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEETP 188 + P+GVVG I+PWN PL++ +WK+A LA G TVV+KPAE T Sbjct: 151 --------------FPDHVPVGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAENTS 196 Query: 189 QSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAG 248 +AL A LAQ+AG P GV+N+VTG G + G A+ HP + KI+FTGS E GR I+ Sbjct: 197 LTALLFAELAQQAGLPAGVLNIVTGDG-ATGAAVVAHPGIQKIAFTGSTEVGRLIREQTA 255 Query: 249 EQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFA 308 K +TLELGGKSP IVF+DA + A+ G ++ NQGQVC AGSR+LVQ + F Sbjct: 256 GSGKSLTLELGGKSPFIVFEDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFI 315 Query: 309 KALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPDKGW 368 L + VG P MG + Q ERV ++QG+ EGA + P G Sbjct: 316 ARLKTRMQKLKVGAPLDKCSDMGALISPVQLERVRSLVEQGVREGAQCHQVVLDTPPGGC 375 Query: 369 FVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLEIS 428 F PT+ + ++A EEIFGPV +SF T +EA+ LAN+S+YGLAA++W+ + ++ Sbjct: 376 FYPPTLLTGVHPAATVASEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAASIWSETIGLA 435 Score = 77.0 bits (188), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 61/200 (30%), Positives = 92/200 (46%), Gaps = 9/200 (4%) Query: 62 WSKLSGLQRGQLLHKLAALVERDTELLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGW 121 W+ S +R Q L+ +A + TE A A G + E R +V +I L W Sbjct: 569 WTSASPHRRAQGLYYIAENLSARTEEFARRIASTTGVTADEARA-EVDASIKRLFTYGAW 627 Query: 122 ANQMEG--RTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTV 179 A++ EG P G ++ + E IGVVG + P PL+ VA L+A G V Sbjct: 628 ADKFEGAVHQPPMRG------VALAMPEAIGVVGVVCPEEKPLLALISLVAPLIAMGNRV 681 Query: 180 VIKPAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEA 239 V+ P+E+ P A L ++ + P GVIN+VTG + L EH +V + G E Sbjct: 682 VVVPSEQAPLVATDLYQVLDTSDLPAGVINIVTGKSAELLPVLAEHDEVEALWVRGPAEF 741 Query: 240 GRAIQRIAGEQFKRVTLELG 259 +R++ KR ++ G Sbjct: 742 SATAERLSTGNLKRCFVDHG 761 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 274 bits (700), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 167/428 (39%), Positives = 237/428 (55%), Gaps = 22/428 (5%) Query: 9 HFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKLSGL 68 +FI G+ G+ ++P+ + A+ +A DV A+ AA A + +W K S Sbjct: 21 NFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKK--SWGKTSPT 78 Query: 69 QRGQLLHKLAALVERDTELLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQMEGR 128 +R +L K+A +E + ELLA + G+ E D+P AI H R A EG Sbjct: 79 ERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGS 138 Query: 129 TIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEETP 188 P + T +Y EP+GVVG I+PWN P+++ WK+A LAAG VV+KPAE+TP Sbjct: 139 ICP----IDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTP 194 Query: 189 QSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAG 248 S + L L + P GV+N+V G+G G+ L + +AKI+FTG GR I + A Sbjct: 195 ASIMVLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYAS 253 Query: 249 EQFKRVTLELGGKSPQIVFDDA------PFEDALFGCTLGLFVNQGQVCAAGSRILVQRS 302 + VTLELGGKSP I F D F+ AL G + +NQG+VC SR+LVQ S Sbjct: 254 QNLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAM-FALNQGEVCTCPSRVLVQES 312 Query: 303 LAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVS 362 + +RF + + A I G+P +G A + Q E++ YI G EGA +LAGG Sbjct: 313 IYERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGG-G 371 Query: 363 RPD------KGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGL 416 R + G++V+PT+F NN M I +EEIFGPV ++ +F EEEA+A+AND+ YGL Sbjct: 372 REELGGDLASGYYVKPTVF-QGNNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGL 430 Query: 417 AATVWTTN 424 A +WT + Sbjct: 431 GAGLWTRD 438 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,671,988 Number of extensions: 71973 Number of successful extensions: 324 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 277 Number of HSP's successfully gapped: 21 Length of query: 518 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 429 Effective length of database: 1,198,455 Effective search space: 514137195 Effective search space used: 514137195 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)