BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0012.p (444 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 569 e-163 gi|300313242|ref|YP_003777334.1| alanine racemase [Herbaspirillu... 329 3e-91 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 226 4e-60 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 211 1e-55 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 198 9e-52 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 569 bits (1467), Expect = e-163, Method: Compositional matrix adjust. Identities = 280/280 (100%), Positives = 280/280 (100%) Query: 165 VINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAGEQFKRVTLELGGKSPQIV 224 VINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAGEQFKRVTLELGGKSPQIV Sbjct: 205 VINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAGEQFKRVTLELGGKSPQIV 264 Query: 225 FDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAAAGITVGDPSQP 284 FDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAAAGITVGDPSQP Sbjct: 265 FDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAAAGITVGDPSQP 324 Query: 285 GVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPDKGWFVQPTIFADANNGMSIAR 344 GVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPDKGWFVQPTIFADANNGMSIAR Sbjct: 325 GVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPDKGWFVQPTIFADANNGMSIAR 384 Query: 345 EEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVGAVGVNCWS 404 EEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVGAVGVNCWS Sbjct: 385 EEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVGAVGVNCWS 444 Query: 405 PLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVTVLLAS 444 PLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVTVLLAS Sbjct: 445 PLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVTVLLAS 484 >gi|300313242|ref|YP_003777334.1| alanine racemase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 329 bits (843), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 159/164 (96%), Positives = 162/164 (98%) Query: 1 VALVSEGVSLKQTLAERWPKGARLHILNGTLPGDELLCLHYGLTPVLNSLEQVRSWQGYA 60 VALVSEGVSLKQTLA+RWPKGARLHILNGTLPGDELLCLHYGLTPVLNSLEQVRSWQGYA Sbjct: 62 VALVSEGVSLKQTLADRWPKGARLHILNGTLPGDELLCLHYGLTPVLNSLEQVRSWQGYA 121 Query: 61 RQLDFPLAAALQVDTGMARLGLAPAEVAQLVGSRSGLAGIAPTFLMSHLVSAEESANPLN 120 RQLDFPLAAALQVDTGMARLGLAPAEVAQL SR+GLAGIAPTFLMSHLVSAEE+ANPLN Sbjct: 122 RQLDFPLAAALQVDTGMARLGLAPAEVAQLTESRAGLAGIAPTFLMSHLVSAEETANPLN 181 Query: 121 QHQLERFVAIRRHWPEIAGCLANSSGIFLGADFHFGCVRPGAAL 164 QHQLERFVAIRRHWPEIAGCLANSSGIFLGADFHFGCVRPGAAL Sbjct: 182 QHQLERFVAIRRHWPEIAGCLANSSGIFLGADFHFGCVRPGAAL 225 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 226 bits (575), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 119/276 (43%), Positives = 163/276 (59%) Query: 165 VINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAGEQFKRVTLELGGKSPQIV 224 ++NV+ G G ++ V K+ F GSP G I + A + LELGGKS I+ Sbjct: 215 LVNVLAGLGQTMVPQAMATWTVKKVIFVGSPATGALIAKAAAARVMPCVLELGGKSANII 274 Query: 225 FDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAAAGITVGDPSQP 284 F+DA A FG +F GQ C AGSR+LVQR + RF + +A A I +G P Sbjct: 275 FEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQRKVYDRFVETVAAGAEKIRLGAPLDD 334 Query: 285 GVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPDKGWFVQPTIFADANNGMSIAR 344 +GP+ + Q+E + R + +GL+ GATL AG ++G+FV+PT+ A A+N M +AR Sbjct: 335 STEVGPINNRKQYEHIQRMVARGLEAGATLAAGHTRYGEEGYFVRPTLLAHASNAMEVAR 394 Query: 345 EEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVGAVGVNCWS 404 EIFGPV I F+ EEEAIA+ANDS++GLA VWT ++ARAHRVAA V G VN + Sbjct: 395 SEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTRDVARAHRVAASVNAGTFWVNSYK 454 Query: 405 PLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVTV 440 ++ P+GG SG GR G+ YT+ K+V V Sbjct: 455 TINVASPFGGFNRSGYGRSSGMEALYDYTQTKSVWV 490 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 211 bits (537), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 122/277 (44%), Positives = 161/277 (58%), Gaps = 4/277 (1%) Query: 165 VINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAGEQFKRVTLELGGKSPQIV 224 V N+V G G VG AL HP V IS TGS AG + + A KRV ELGGKSP +V Sbjct: 206 VFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVKRVAQELGGKSPNLV 265 Query: 225 FDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAAAGITVGDPSQP 284 DA E A+ N GQ C+A +R++V R+ R + A + VGDP+ Sbjct: 266 LPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELALATLAQMKVGDPNDA 325 Query: 285 GVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPD---KGWFVQPTIFADANNGMS 341 GP+A +AQF RV + I+ GL +GA LLAGG RPD +G++ +PTIF++ + M Sbjct: 326 ATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYYARPTIFSEVHTDMV 385 Query: 342 IAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVGAVGVN 401 IA+EEIFGPV I+ +DT EEAIA+AND+ YGL A V + + VAA ++ G V +N Sbjct: 386 IAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRAVAARIQSGQVHLN 445 Query: 402 CWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTV 438 + D P+GG K SG GRE G+ G Y E K + Sbjct: 446 -YPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKAI 481 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 198 bits (503), Expect = 9e-52, Method: Compositional matrix adjust. Identities = 115/279 (41%), Positives = 159/279 (56%), Gaps = 5/279 (1%) Query: 166 INVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAGEQFKRVTLELGGKSPQIVF 225 +N+VTGYG+ G L +HPDV ISFTGSP G+ + R A E VTLELGGKSPQIVF Sbjct: 197 LNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHHTPVTLELGGKSPQIVF 256 Query: 226 DDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAAAGITVGDPSQPG 285 +DA E L + N GQ C+AGSR+LVQR ++ + L+ + + VG SQ Sbjct: 257 EDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERLSGLFSQLQVGS-SQMD 315 Query: 286 VRMGPVAKKAQFERVNRYIQQGLDEGATLLAGG---VSRPDKGWFVQPTIFADANNGMSI 342 + GP+ +K Q ERV ++ +G L A G P G++ P + D + Sbjct: 316 LDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPTGGFYQAPALLRDVPPRHRL 375 Query: 343 AREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVGAVGVNC 402 A+EE+FGPV + F+ E AIALAN + YGL A+VWT + R R+A ++ G V +N Sbjct: 376 AQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQMRLARAIRSGQVFINN 435 Query: 403 W-SPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVTV 440 + + LP+GG+K SG GRE G +T KT+ + Sbjct: 436 YGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAI 474 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,455,385 Number of extensions: 64364 Number of successful extensions: 270 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 249 Number of HSP's successfully gapped: 20 Length of query: 444 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 356 Effective length of database: 1,203,190 Effective search space: 428335640 Effective search space used: 428335640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)