BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0003.p (377 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC t... 750 0.0 gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 162 3e-41 gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 132 7e-32 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 94 1e-20 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 67 3e-12 >gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 750 bits (1937), Expect = 0.0, Method: Compositional matrix adjust. Identities = 369/377 (97%), Positives = 372/377 (98%) Query: 1 VLAGVLLLWAFMQAGWAQERKTIALGFTGRLSEPIAQSALQGAQLAIDEANALAAKRRSP 60 VLAGVLLLWAFMQAGWAQERKTIALGFTGRLSEPIAQSALQGAQLAIDEANALAAKRRSP Sbjct: 9 VLAGVLLLWAFMQAGWAQERKTIALGFTGRLSEPIAQSALQGAQLAIDEANALAAKRRSP 68 Query: 61 LRFALLPQDDHGNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQIPQIGFTA 120 LRF LLPQDDHGNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQIPQIGFTA Sbjct: 69 LRFELLPQDDHGNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQIPQIGFTA 128 Query: 121 TSSQWTGMGNRMPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRALADTLVEN 180 TSSQWTGMGNRMPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRALADTLVEN Sbjct: 129 TSSQWTGMGNRMPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRALADTLVEN 188 Query: 181 LGGSAQQIALARYQVGRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGANRSRTTA 240 LGGSAQQIALARYQVGRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGANR+RTTA Sbjct: 189 LGGSAQQIALARYQVGRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGANRTRTTA 248 Query: 241 RLLLTGGASNLSLPAPLELRLHVLEYEIARPHCPQWKAVTQAYQRRFGAAPTTYSFYAYD 300 RLLLTGGASNLSLPAPLELRLHVLEYEIARPHCPQWK VTQ YQRRFGAAPTTYSFYAYD Sbjct: 249 RLLLTGGASNLSLPAPLELRLHVLEYEIARPHCPQWKVVTQTYQRRFGAAPTTYSFYAYD 308 Query: 301 AASVLTAAIAQAGSDNGDRIATALHRMRHAGLHGTLSFDATGANAHPRYTLLRYQERWQA 360 AASVLTAAI QAGSDNGD+IATALHRMRHAGLHGTLSFDATGANAHPRYTLLRYQERWQA Sbjct: 309 AASVLTAAIVQAGSDNGDKIATALHRMRHAGLHGTLSFDATGANAHPRYTLLRYQERWQA 368 Query: 361 VEFFAPGNANARQEACS 377 VE FAPG+ANARQEACS Sbjct: 369 VEVFAPGSANARQEACS 385 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 162 bits (411), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 119/363 (32%), Positives = 172/363 (47%), Gaps = 25/363 (6%) Query: 18 QERKTIALGFTGRLSEP---IAQSALQGAQLAIDEANALAAKRRSPLRFA-------LLP 67 QE T+A+GF G LS P + +S G QLA+ EAN R LR L+ Sbjct: 35 QETATLAIGFAGPLSGPSGGVGKSMQNGVQLALAEAN------RRGLRIGGKSWQLRLIA 88 Query: 68 QDDHGNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQIPQIGFTATSSQWTG 127 QDD + A +AR V+++V V+G W+S +LA A +Y A + Q+ +A S + T Sbjct: 89 QDDRADPATAEFVARSLVQQQVIAVVGHWTSGTSLAAAPVYHRAGVIQVTPSAMSRRLTA 148 Query: 128 MGNRMPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRALADTLVENLGGSAQQ 187 M FR + L A A L+ R I I + + LA+ Q Sbjct: 149 MSYPRIFRTIPNNESLGRLAAGYAVDKLETRTIVTIDDRTPFGQGLAEQFARTASERGAQ 208 Query: 188 IALARYQVGRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGANRSRTTARLLLTGG 247 + + RY V +++DF+AAL A Q D+I F GL QA R + ARL+ + G Sbjct: 209 V-VGRYSVSDKTSDFNAALLEARKLQPDLIFFGGLDWQAGVLAKSIRRLKLHARLMASPG 267 Query: 248 ASNLSLP-----APLELRLHVLEYEIARPHCPQWKAVTQAYQRRFGAAPTTYSFYAYDAA 302 L LP P VLE + P WK+ Q Y F + Y+ +AY+AA Sbjct: 268 --TLGLPFLMRAGPDANGALVLEPGPPQDKMPGWKSFRQRYSENFDSDMDLYAVFAYEAA 325 Query: 303 SVLTAAIAQAGSDNGDRIATALHRMRHAGLHGTLSFDATGANAHPRYTLLRYQE-RWQAV 361 + I QAGS + +RIA A+HR+R G+ G ++F+ G P +T+ ++ RWQ V Sbjct: 326 QAIIQGIRQAGSADPERIAEAMHRLRFEGVSGPVAFNEEGDLLQPSFTMYEVKDQRWQPV 385 Query: 362 EFF 364 F Sbjct: 386 ARF 388 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 132 bits (331), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 102/360 (28%), Positives = 169/360 (46%), Gaps = 19/360 (5%) Query: 9 WAFMQAGWAQERKTIALGFTGRLSEP---IAQSALQGAQLAIDEANA----LAAKRRSPL 61 AF A ++QE + + G LS P + + GA++A+DE NA + K+ + Sbjct: 16 LAFAGAAYSQE--VVKIAHVGPLSGPNAHMGKDNENGARMAVDELNAKGFTIGGKK---V 70 Query: 62 RFALLPQDDHGNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQIPQIGFTAT 121 +F L+ +DD + A +A V +KVA VIG +S + + IY A IPQ+ +AT Sbjct: 71 KFELVGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDAGIPQVSPSAT 130 Query: 122 SSQWTGMGNRMPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRALADTLVENL 181 + ++T G + FRVV +L A L + A + L ++I VI AY + +AD + Sbjct: 131 NPKYTQQGFKTAFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQGVADEFAKGA 190 Query: 182 GGSAQQIALARYQVGRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGANRSRTTAR 241 + I +Y ++TDF+A L + + + DV+ F G+ + + +A+ Sbjct: 191 KAAGGTIVGTQY-TNDKATDFNAILTSLKGKKPDVVFFGGMDAVGGPMLRQMKQLGISAK 249 Query: 242 LLLTGGASNLSLPAPLELRL---HVLEYEIARPHCPQWKAVTQ---AYQRRFGAAPTTYS 295 + G SLP L V+ E K + AY+++FG + Sbjct: 250 FMGGDGICTGSLPGLAGDGLGDDQVICAEAGGVDEAGKKGMDDFRAAYKKKFGIDVVYNA 309 Query: 296 FYAYDAASVLTAAIAQAGSDNGDRIATALHRMRHAGLHGTLSFDATGANAHPRYTLLRYQ 355 YAYDA + A+A+AGS + + L ++ H G+ G ++FDA G TL Y+ Sbjct: 310 AYAYDATMTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGDIKDGSLTLYTYK 369 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 94.4 bits (233), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 82/373 (21%), Positives = 157/373 (42%), Gaps = 31/373 (8%) Query: 1 VLAGVLLLWAFMQAGWAQERKTIALGFTGRLSEPIAQSALQ---GAQLAIDEANA----L 53 + A + L+ AF AQE + + +GF+ L+ P A + G +AI+ NA + Sbjct: 11 IAASLALIPAF---AMAQETQVVKIGFSSPLTGPQASAGKDNQGGLMMAIERLNAQPITV 67 Query: 54 AAKRRSPLRFALLPQDDHGNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQI 113 K+ ++F ++ +DD + V +A+ + V ++GP++S + + +Y A I Sbjct: 68 GGKK---IKFDVIAEDDQADPKSGVAVAQKLADQGVKAIVGPYNSGVTIPASRVYNDAGI 124 Query: 114 PQIGFTATSSQWTGMGNRMPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRAL 173 + A++ + T G FRV +L +A A + L+ +R+ VI AY + L Sbjct: 125 -VVATVASNPKITQQGFATLFRVAASDSQLGGKMALYAAKELKFKRVAVIDDRTAYGQGL 183 Query: 174 ADTLVENLGGSAQQIALARYQVGRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGA 233 A ++ + + + ++TDF A L + + + D + G PQ Sbjct: 184 AQEFIKVAKANGIDVVSTDF-TNDKATDFTAILTSIKGKKPDAVFLGGYAPQGGPIKRQM 242 Query: 234 NRSRTTARLLLTGGASNLSLPAPLELRLHVLEYEIARPHCPQ----------WKAVTQAY 283 + L+ G + P L + + +C Q K + Y Sbjct: 243 KQLGVDVPLM---GGDGICSPEMGRLGGDAIGESV---YCTQGGTMLDKAKEGKVFSDEY 296 Query: 284 QRRFGAAPTTYSFYAYDAASVLTAAIAQAGSDNGDRIATALHRMRHAGLHGTLSFDATGA 343 Q+++ TY+ YD ++ A+ QA S + + AL ++ + G+ G FDA Sbjct: 297 QKKYNRPAETYAVSFYDGMMLIAQAMKQANSVDPKQFGPALAKISYKGVAGQYDFDANHD 356 Query: 344 NAHPRYTLLRYQE 356 T+ R+++ Sbjct: 357 LKQSPVTVYRFKD 369 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 66.6 bits (161), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 90/353 (25%), Positives = 149/353 (42%), Gaps = 29/353 (8%) Query: 23 IALGFTGRLSEPIAQSAL---QGAQLAIDEANALAAKRRSPLRFALLPQDDHGNENFAVN 79 I LG L+ P A+ + G LA +E NA + AL+ +D+ G + A+N Sbjct: 28 IKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVNGD--KLALVIEDEQGKKEEAIN 85 Query: 80 IARYYV-KEKVAGVIGPWSSDAALATAAIYESAQIPQIGFTATSSQWTGMGNRMPFRVVG 138 + + + ++KV V GP S++A A I +A++ G + T+ T MG PF Sbjct: 86 VFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTSNTADGITAMG---PFTFRN 142 Query: 139 GTRE--LAATLAEVATRSLQGRRIFVIQ-SDAAYSRALADTLVENLGGSAQQIALARYQV 195 E + + A + +++ VI +D A++++ D L Q+I + + Sbjct: 143 SVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFKATL--EHQKIPVTTTEA 200 Query: 196 -GRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGANRSRTTARLLLTGGASNLSLP 254 + DF A L +A D IV L +A I ++R G + L+ P Sbjct: 201 YAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANII---LQTRALGMKQPFIGGNGLNSP 257 Query: 255 APLELRLHVLEYEI------ARPHCPQWKAVTQAYQRRFGAAPTTYSFYAYDAASVLTAA 308 E+ + + A P KA AY+ +FGA P ++ AYDA ++ A Sbjct: 258 KLFEIAKDAGDNTLMGSPWSAENQTPANKAFITAYKAKFGADPDQFAAQAYDAMYIVADA 317 Query: 309 IAQ---AGSDNGDRIA--TALHRMRHAGLHGTLSFDATGANAHPRYTLLRYQE 356 + +G+ DR A AL ++ G G +F A A A + QE Sbjct: 318 LKNVKLSGNLAKDRDALRAALPAVKIDGATGKFAFRAAPAVAGKQVGFDADQE 370 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.131 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,172,445 Number of extensions: 47335 Number of successful extensions: 211 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 205 Number of HSP's successfully gapped: 9 Length of query: 377 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 290 Effective length of database: 1,207,925 Effective search space: 350298250 Effective search space used: 350298250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)