BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0174.p (265 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 157 8e-40 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 154 1e-38 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 139 2e-34 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 136 2e-33 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 132 2e-32 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 157 bits (397), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 100/258 (38%), Positives = 146/258 (56%), Gaps = 13/258 (5%) Query: 3 RLKDKVAIITGASSGFGRGIGFAFANQGAKIVVSDVHEAPNRGGFEGNPELTTTAAIRET 62 RL+DKVAI+TG SGFG GI A+A +GA IVV+D+ EA GG L I+ Sbjct: 2 RLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEA---GG------LRVVEEIKAA 52 Query: 63 GGQAYYVNCDVTRADDVAKLVAATVERYGRLDIIVNNAGIYRAGKLAHEFAVEELDACFD 122 GG+A + DV++ D+ +L+A +E +G+LDI+VNNAG + E +E D + Sbjct: 53 GGRAVFAKADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYA 112 Query: 123 VNVKGTWFGCQEAIKQFLKQGDGGNIVNIVSTAGLQGHPKQSVYNISKGAAANLTRCLAI 182 VNVK + + + F +Q GG +NI STAG++ P + YN SKGA ++ +A Sbjct: 113 VNVKSIFLSAKTFVPYF-RQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAA 171 Query: 183 EYGQNKIRVNGICPTYAKTSLTRALF---DDKDFDKVFTEAIPLKRWGEVDDIASLAVFL 239 E G +KIRVN + P + T L D + K F IP+ R+ +DIA+ ++L Sbjct: 172 ELGPDKIRVNCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYL 231 Query: 240 ASDESSYIHGDLIRIDGG 257 SDE+ ++ G I +DGG Sbjct: 232 GSDEAEFVTGVCIEVDGG 249 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 154 bits (388), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 89/257 (34%), Positives = 146/257 (56%), Gaps = 14/257 (5%) Query: 1 MTRLKDKVAIITGASSGFGRGIGFAFANQGAKIVVSDVHEAPNRGGFEGNPELTTTAAIR 60 + R + KV I+TGA+SG G F+++GA+++++D A F+ P T A R Sbjct: 9 LQRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAA--R 66 Query: 61 ETGGQAYYVNCDVTRADDVAKLVAATVERYGRLDIIVNNAGIYRAGKLAHEFAVEELDAC 120 ET DV+ + V +LV +ER+G+LD++V++AG++ G + E + E+ Sbjct: 67 ET---------DVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVT-EVSPEDWHRV 116 Query: 121 FDVNVKGTWFGCQEAIKQFLKQGDGGNIVNIVSTAGLQGHPKQSVYNISKGAAANLTRCL 180 NV G ++G +EA+ K G IVN+ S +GL S YN SKGA NLTR + Sbjct: 117 QATNVNGVFYGAREALPHLEKTR--GCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAM 174 Query: 181 AIEYGQNKIRVNGICPTYAKTSLTRALFDDKDFDKVFTEAIPLKRWGEVDDIASLAVFLA 240 A+++G+ +R+N +CP+ T++T + DD F E I L R + +IA++ FLA Sbjct: 175 ALDFGRKGVRINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLA 234 Query: 241 SDESSYIHGDLIRIDGG 257 S ++S+++G + +DGG Sbjct: 235 SPDASFVNGVNLPVDGG 251 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 139 bits (350), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 84/256 (32%), Positives = 128/256 (50%), Gaps = 11/256 (4%) Query: 4 LKDKVAIITGASSGFGRGIGFAFANQGAKIVVSDVHEAPNRGGFEGNPELTTTAAIRETG 63 L+ K+A+ITG+S G G + A GA IV++ G + A +R G Sbjct: 17 LEGKLALITGSSGGIGLALARGMAQAGAAIVLN---------GRDAGKLAAVAAQLRAEG 67 Query: 64 GQAYYVNCDVTRADDVAKLVAATVERYGRLDIIVNNAGIYRAGKLAHEFAVEELDACFDV 123 + + D T +++V V G + I+VNNAGI R L EF + Sbjct: 68 HTIHESSFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPL-EEFKESDWHDLIST 126 Query: 124 NVKGTWFGCQEAIKQFLKQGDGGNIVNIVSTAGLQGHPKQSVYNISKGAAANLTRCLAIE 183 N+ +F Q + + +G G I+NI S G P + Y +KGA T+ +AI+ Sbjct: 127 NLNSVFFAAQAVARHMIPRG-AGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAID 185 Query: 184 YGQNKIRVNGICPTYAKTSLTRALFDDKDFDKVFTEAIPLKRWGEVDDIASLAVFLASDE 243 +G+ I+VNG+ P Y KT + AL +D F P +RWG+V+D+ AVFLASD Sbjct: 186 WGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDA 245 Query: 244 SSYIHGDLIRIDGGET 259 S +++G ++ +DGG T Sbjct: 246 SRFVNGHILYVDGGVT 261 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 136 bits (342), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 91/257 (35%), Positives = 128/257 (49%), Gaps = 17/257 (6%) Query: 3 RLKDKVAIITGASSGFGRGIGFAFANQGAKIVVSDVHEAPNRGGFEGNPELTTTAAIRET 62 R +DKV ++TG S G G FA +GA++ VS G A+RE Sbjct: 6 RFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVS------------GRRADKLEQAVREI 53 Query: 63 GGQAYYVNCDVTRADDVAKLVAATVERYGRLDIIVNNAGIYRA-GKLAHEFAVEELDACF 121 G A V D + DD+ +L A +GRLD++ NAG K E+ D F Sbjct: 54 GNGAVGVPGDAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLF 113 Query: 122 DVNVKGTWFGCQEAIKQFLKQGDGGNIVNIVSTAGLQGHPKQSVYNISKGAAANLTRCLA 181 ++NV+G F Q+A+ + QG G IV S AG++G P QS+YN SK A + R Sbjct: 114 NLNVRGLLFSVQKALP-LMSQG--GAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWT 170 Query: 182 IEYGQNKIRVNGICPTYAKTSLTRALFDDKD-FDKVFTEAIPLKRWGEVDDIASLAVFLA 240 + + IRVN + P +T L R + + + V + +PL R GE D+I +FLA Sbjct: 171 ADLKERGIRVNVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLA 230 Query: 241 SDESSYIHGDLIRIDGG 257 S ESSYI G + +DGG Sbjct: 231 SAESSYIAGVELFVDGG 247 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 132 bits (333), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 81/252 (32%), Positives = 135/252 (53%), Gaps = 14/252 (5%) Query: 7 KVAIITGASSGFGRGIGFAFANQGAKIVVSDVH-EAPNRGGFEGNPELTTTAAIRETGGQ 65 +VA++TG + G G I A +G ++V+D++ +A ++ T A+ G + Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASK----------TADALVAAGHR 58 Query: 66 AYYVNCDVTRADDVAKLVAATVERYGRLDIIVNNAGIYRAGKLAHEFAVEELDACFDVNV 125 A + D+ + +A A + YGR D++VNNAG+ R ++ +E +VNV Sbjct: 59 AAPLQMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFL-DYPLENWLQTMNVNV 117 Query: 126 KGTWFGCQEAIKQFLKQGDGGNIVNIVSTAGLQGHPKQSVYNISKGAAANLTRCLAIEYG 185 G Q A + +KQ G IVNI S +G++ ++ Y SK A LTR +AIE Sbjct: 118 TGVLLAGQHAARLMVKQ-QWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELA 176 Query: 186 QNKIRVNGICPTYAKTSLTRALFDDKDFDKVFTEAIPLKRWGEVDDIASLAVFLASDESS 245 Q I VN + P T +T+A+ ++ + + +P++R+G D+IAS +LA++ES+ Sbjct: 177 QYGITVNSVAPGPVDTPMTQAMHSEQTRESYY-RLVPMRRYGTTDEIASAVCYLAAEESA 235 Query: 246 YIHGDLIRIDGG 257 YI G +I +DGG Sbjct: 236 YITGHVIPVDGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 816,494 Number of extensions: 33746 Number of successful extensions: 268 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 164 Number of HSP's successfully gapped: 47 Length of query: 265 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 181 Effective length of database: 1,222,130 Effective search space: 221205530 Effective search space used: 221205530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)