BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0173.p (169 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 100 1e-22 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 88 5e-19 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 87 7e-19 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 87 1e-18 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 82 3e-17 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 99.8 bits (247), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 56/155 (36%), Positives = 90/155 (58%), Gaps = 11/155 (7%) Query: 14 EQYDFTMNVNAKGIWLGSKYAITQFMKQEPITTSTGAKLRGRVVNIAYIGGLVGLALEPA 73 E + TMNVN G+ L ++A +KQ+ GR+VNI+ I G+ A A Sbjct: 107 ENWLQTMNVNVTGVLLAGQHAARLMVKQQ----------WGRIVNISSISGIRAGAGRTA 156 Query: 74 YCASKGAVVNLTRELALDFAAERINVNAVCPGFLATAMVRSFLEKEDTNKLLHDLTPWPR 133 Y SK AV+ LTR++A++ A I VN+V PG + T M ++ + E T + + L P R Sbjct: 157 YGTSKAAVIGLTRQMAIELAQYGITVNSVAPGPVDTPMTQA-MHSEQTRESYYRLVPMRR 215 Query: 134 VGSAEDVARAVTFLASDDAEWMTGSMLTVDGAFTA 168 G+ +++A AV +LA++++ ++TG ++ VDG F A Sbjct: 216 YGTTDEIASAVCYLAAEESAYITGHVIPVDGGFVA 250 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 87.8 bits (216), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 58/171 (33%), Positives = 93/171 (54%), Gaps = 20/171 (11%) Query: 7 NIIEETEEQYDFTMNVNAKGIWLGSKYAITQFMKQEPITTSTGAKLRGRVVNIAYIGGLV 66 NI+E ++ +DF+ N+NAK ++ + + + AK G +VNIA V Sbjct: 93 NILECDDKAWDFSFNLNAKAMFHTIRAVLPGML----------AKKAGSIVNIASAASSV 142 Query: 67 -GLALEPAYCASKGAVVNLTRELALDFAAERINVNAVCPGFLATAMV--RSFLEKEDTNK 123 G+A AY ASK AVV LT+ +A DF A+ I NA+CPG + + + R + ++T K Sbjct: 143 KGVANRFAYGASKAAVVGLTKSVAADFVAQGIRCNAICPGTIESPSLNQRISTQAKETGK 202 Query: 124 LLHDL-------TPWPRVGSAEDVARAVTFLASDDAEWMTGSMLTVDGAFT 167 ++ P R+G AE+VA +LASD++ + TGS+ +DG ++ Sbjct: 203 SEEEVRAAFVGRQPMGRIGKAEEVAALALYLASDESNFTTGSIHMIDGGWS 253 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 87.0 bits (214), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 58/165 (35%), Positives = 79/165 (47%), Gaps = 17/165 (10%) Query: 7 NIIEETEEQYDFTMNVNAKGIWLGSKYAITQFMKQEPITTSTGAKLRGRVVNIAYIGGLV 66 N+ E + E + N G++ G++ A+ K RG +VN+A + GL Sbjct: 104 NVTEVSPEDWHRVQATNVNGVFYGAREALPHL-----------EKTRGCIVNVASVSGLA 152 Query: 67 GLALEPAYCASKGAVVNLTRELALDFAAERINVNAVCPGFLATAMVRSFLEKEDTNKLLH 126 AY ASKGAV NLTR +ALDF + + +NAVCP TAM + D LL Sbjct: 153 ADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVCPSLTHTAMT---ADMADDPPLLD 209 Query: 127 DLTPWPRVGSAED---VARAVTFLASDDAEWMTGSMLTVDGAFTA 168 +G D +A +TFLAS DA ++ G L VDG A Sbjct: 210 KFAERIALGRGADPLEIAAVITFLASPDASFVNGVNLPVDGGLMA 254 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 86.7 bits (213), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 47/114 (41%), Positives = 66/114 (57%) Query: 53 RGRVVNIAYIGGLVGLALEPAYCASKGAVVNLTRELALDFAAERINVNAVCPGFLATAMV 112 RG +VN A + G L P Y ASKG V LT+ LA+ +AA+ I VNAV PG++AT + Sbjct: 125 RGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLT 184 Query: 113 RSFLEKEDTNKLLHDLTPWPRVGSAEDVARAVTFLASDDAEWMTGSMLTVDGAF 166 ++ + + TP R G EDV + V FL S A +MTG+++ VDG + Sbjct: 185 QALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGGY 238 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 81.6 bits (200), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 49/154 (31%), Positives = 79/154 (51%), Gaps = 12/154 (7%) Query: 13 EEQYDFTMNVNAKGIWLGSKYAITQFMKQEPITTSTGAKLRGRVVNIAYIGGLVGLALEP 72 +E++D + N + S+ + MK GR++NI + G G + Sbjct: 107 DEEWDSVIATNLTAVGRLSRAVLRGMMKART----------GRIINITSVVGEAGNPGQM 156 Query: 73 AYCASKGAVVNLTRELALDFAAERINVNAVCPGFLATAMVRSFLEKEDTNKLLHDLTPWP 132 Y A+K V ++R LA + + I VN V PGF+ T M R+ E++ +L + P Sbjct: 157 NYAAAKAGVAGMSRALAREIGSRNITVNCVAPGFIDTDMTRALNEQQSA-AILQQI-PLG 214 Query: 133 RVGSAEDVARAVTFLASDDAEWMTGSMLTVDGAF 166 R+G+AE++A AV FLAS A ++TG+ L V+G Sbjct: 215 RLGAAEEIAYAVAFLASPQAAYITGTTLNVNGGM 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 482,795 Number of extensions: 17123 Number of successful extensions: 128 Number of sequences better than 1.0e-08: 28 Number of HSP's gapped: 110 Number of HSP's successfully gapped: 28 Length of query: 169 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 90 Effective length of database: 1,245,805 Effective search space: 112122450 Effective search space used: 112122450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)