BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0159.p (531 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312041|ref|YP_003776133.1| acyl-CoA synthetase/AMP-acid li... 260 1e-70 gi|300311658|ref|YP_003775750.1| non-ribosomal peptide synthetas... 137 2e-33 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 96 5e-21 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 92 8e-20 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 73 4e-14 >gi|300312041|ref|YP_003776133.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 539 Score = 260 bits (665), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 182/501 (36%), Positives = 245/501 (48%), Gaps = 55/501 (10%) Query: 43 GDRIAICGPKSTDLCVLMLATWGVGAVTVPIFSGLKTKQVRHILSD--------SEP--- 91 G+R+A+ K + + + G V VPI LK QV HIL D SEP Sbjct: 54 GERVAVYLEKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCAVRVLVSSEPRHP 113 Query: 92 -------------TLVFAGMRDFAMVAQAHGGTTHPLEHFGTQAHVTSAAPQCGDFYASA 138 T+V A A L A + AAP G A Sbjct: 114 ALADRLSQCPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFL-QAAPGPGSRRAHR 172 Query: 139 AAVTEAAPALIVYTSGSTGQPKGVVFSRSNLVLGSRSVARFMHLTNDDRVLCVLPFSFDA 198 +A A I+YTSGSTGQPKGVV S NLV G++SVA ++ DR+L VLP SFD Sbjct: 173 R--IDADIAAILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDY 230 Query: 199 GLNQFLSALTVGATVVLIDFVHASQIEECCATHGITSITAVPGLWSKKPYLGRTAEQGKA 258 GL+Q +A GA+V L++++ I A+ GIT + VP LW +Q Sbjct: 231 GLSQLSTAFLSGASVTLLNYLAPRDILHAVASEGITGLAGVPPLW----------QQLAG 280 Query: 259 THWQXDASRRSVRRIGNTGGHLQVELLKQLQNIFSNADVFLMYGFTEAFRATYLDPGTVA 318 W S +R + N+GG L +L QL+ A VFLMYG TEAFR+TYL P + Sbjct: 281 LPWPQGCS---LRYLTNSGGALPRSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELD 337 Query: 319 RKPHSVGRPIPFASVAVVTEEGRLCAPDEIGELVQFGPLVTLGYHNLPEENAVKFRPLPV 378 R+P S+GR IP A + VV +G CAP E+GELV G LV GY N P A +FRP P Sbjct: 338 RRPDSIGRAIPNAEIMVVRPDGSPCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAP- 396 Query: 379 GLVDELMGPRGRHYVFDPAHATAAAWSGDLVSIDEDGDIVYRGRKDDLIKVNGFRICAGD 438 GR A WSGD V +DE G + + GR DD+IKV+G+RI + Sbjct: 397 ----------GREAAL--GTPELAVWSGDSVRMDEQGYLYFVGRADDMIKVSGYRISPHE 444 Query: 439 IEDACVEAGLAIAIAIGMTGGEEEGVVVFGKCADASTGERE-IRERLRFNLPTYMIPRAI 497 +E+ GLA A+ ++G V + T E +++ R +LP+YM+P A+ Sbjct: 445 VEEVLHATGLADQFAVVGVAHPQQGQAVVAVVPSSCTASLEAVQQACRAHLPSYMLPAAL 504 Query: 498 F-LIDDFPLTSNGKFDRQALR 517 + P + NGK DR+ ++ Sbjct: 505 HRWPGELPRSPNGKLDRKLMQ 525 >gi|300311658|ref|YP_003775750.1| non-ribosomal peptide synthetase [Herbaspirillum seropedicae SmR1] Length = 9160 Score = 137 bits (346), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 143/530 (26%), Positives = 224/530 (42%), Gaps = 50/530 (9%) Query: 5 EIDDGFCIVYKSEAHKGGQLRERVNAGMENLRSLGVRSGDRIAICGPKSTDLCVLMLATW 64 + D +V + ++ G+L R N +L SLGV + R+ + +S D+ V +LA Sbjct: 8274 QTPDAIALVLEEQSLSYGELNVRANRLAHHLMSLGVGAEQRVGVAVERSFDMVVGLLAVM 8333 Query: 65 GVGAVTVPIFSGLKTKQVRHILSDSEPTLVFAGMRDFAMVA-QAHGGTTHPLEHFGTQAH 123 GA VP+ +++ ++++DS ++ R ++A QA G A Sbjct: 8334 KAGAAYVPLDLDYPPERLAYMVADSRIRVLLTHARARRLLAAQAQPGLRCLALDDADLAA 8393 Query: 124 VTSAAPQCGDFYASAAAVTEAAPALIVYTSGSTGQPKGVVFSRSNLVLGSRSVARFMHLT 183 + P + AV A ++YTSGSTGQPKGV S LV+ ++ A +T Sbjct: 8394 QLAGLPA----HDPVVAVAPQQMAYVIYTSGSTGQPKGVAVSHGPLVMHCQATAALYEMT 8449 Query: 184 NDDRVLCVLPFSFDAGLNQFLSALTVGATVVLIDFVHASQIEECCATHGITSITAVPGLW 243 R L + FSFD ++++ L GA +VL S + A H AV Sbjct: 8450 PQWRELQFMSFSFDGAHERWITTLISGAALVLRGPEMWSIEQSWQALHQQRVSNAV---- 8505 Query: 244 SKKPYLGRTAEQGKATHWQXDASRRSVRRIGNTGGHLQVELLKQLQNIFSNADVFLMYGF 303 YLG+ A+ G+ DA + G G + ++++ + + YG Sbjct: 8506 FPPAYLGQLAQWGEQ---HGDAPPLELYVFG--GEAMPRASFERVRRHLAPRLLINGYGP 8560 Query: 304 TEAFRATYLDPGTVARKPH------SVGRPIPFASVAVVTEEGRLCAPDEIGELVQFGPL 357 TE + GT A +G+P+ + ++ + AP E GEL G Sbjct: 8561 TETVVTPLIWKGTSAAGVDFDGPFVPIGKPVGRRNAYILDACLQPVAPGEAGELYIGGDG 8620 Query: 358 VTLGYHNLPEENAVKFRPLPVGLVDELMGPRGRHYVFDPAHATAAAWSGDLVSIDEDGDI 417 + GY P ++A +F P G P R Y +GDL DG++ Sbjct: 8621 LARGYLERPAQSAERFVADPFG------EPGARMYR-----------TGDLARWLPDGNV 8663 Query: 418 VYRGRKDDLIKVNGFRICAGDIEDAC-VEAGLAIAIAIGMTGGEEEGVV--------VFG 468 Y GR D +K+ GFRI G+IE + G+A A + + G+ E V Sbjct: 8664 EYLGRLDHQVKIRGFRIELGEIEALLRRQPGVAEAAVVVRSDGQGEETVRQLVAYVGAPQ 8723 Query: 469 KCADASTGERE----IRERLRFNLPTYMIPRAIFLIDDFPLTSNGKFDRQ 514 A T +R+ +R LR LP YM+P L+ P+T NGK DR+ Sbjct: 8724 AVAAPETAQRDFSDSLRAALRAGLPEYMLPAHFVLLAALPVTHNGKLDRK 8773 Score = 115 bits (289), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 140/550 (25%), Positives = 210/550 (38%), Gaps = 57/550 (10%) Query: 2 QLFEIDDGFCIVYKSEAHKGGQLRERVNAGMENLRSLGVRSGDRIAICGPKSTDLCVLML 61 Q I D +V + QL N L GV DRI + P+S ++ V +L Sbjct: 5670 QAAAIPDAVAVVCGPQQMSYAQLNRAANRLAHRLLQQGVGLEDRIGLAAPRSLEMIVGLL 5729 Query: 62 ATWGVGAVTVPIFSGLKTKQV--------RHILSDSEPTLVFAGMRDFAMVAQAHGGTTH 113 A GA VP+ ++ RH D + G R A AH + Sbjct: 5730 AILKAGAAYVPLDPDYPADRLAYMVQACRRHPGVDGRARVSLPGPRK----ACAHWRSPR 5785 Query: 114 PLEHFGTQAHVTSAAPQCGDFYASAAAVTEAAPALIVYTSGSTGQPKGVVFSRSNLVLGS 173 P AH + A I+YTSGSTG+PKG S +N++ Sbjct: 5786 PWADDAAPAHNPGVVLHPDNL------------AYIIYTSGSTGRPKGAQLSHANVLRLL 5833 Query: 174 RSVARFMHLTNDDRVLCVLPFSFDAGLNQFLSALTVGATVVLIDFVHASQIEECCATHGI 233 A + +D ++FD + + AL G +V++ F + E+ A Sbjct: 5834 GQTAAWFDFGREDVWTLFHSYAFDFSVWEIFGALCYGGRLVVVPFAVSRSPEDFLALLVE 5893 Query: 234 TSITAVPGLWSKKPYLGRTAEQGKATHWQXDASRRSVRRIGNTGGHLQVELLKQLQNIFS 293 +T + ++ P R Q A Q + ++R + G L+ E+L+ F Sbjct: 5894 QRVTVL----NQTPSAFRQLLQLPALR-QLPSEALALRTVIFGGEALEPEMLRPWITQFG 5948 Query: 294 NADVFL--MYGFTE-----AFRATYLDPGTVARKPHSVGRPIPFASVAVVTEEGRLCAPD 346 + L MYG TE +R +R P +G IP + V+ ++ L Sbjct: 5949 DQRPALINMYGITETTVHVTYRRLLRADLDASRSP--IGAAIPDLGLYVLDQQLNLAPAG 6006 Query: 347 EIGELVQFGPLVTLGYHNLPEENAVKFRPLPVGLVDELMGPRGRHYVFDPAHATAAAWSG 406 GEL G + GY N +A +F P E R Y +G Sbjct: 6007 VPGELYVSGAGLARGYLNRAALSAERFVANPFARAGE---EGSRLYR-----------TG 6052 Query: 407 DLVSIDEDGDIVYRGRKDDLIKVNGFRICAGDIEDACV-EAGLAIAIAIGMTGGEEEGVV 465 DLV DG + Y GR D +K+ GFRI G+IE + + G+ A + +V Sbjct: 6053 DLVRWRHDGQMDYLGRIDQQVKIRGFRIELGEIEAQLLAQPGVGAATVMVRQDAATPRLV 6112 Query: 466 VF----GKCADASTGEREIRERLRFNLPTYMIPRAIFLIDDFPLTSNGKFDRQALRALAL 521 + A + +R L LP YM+P A+ ++ PL NGK DR+AL A L Sbjct: 6113 GYLSPQAGIAPEALDTGALRTALGAVLPDYMVPAALVMLPALPLNINGKVDRKALPAPEL 6172 Query: 522 QQSEGLASAQ 531 A+ Q Sbjct: 6173 AARTAYAAPQ 6182 Score = 104 bits (260), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 118/395 (29%), Positives = 171/395 (43%), Gaps = 47/395 (11%) Query: 147 ALIVYTSGSTGQPKGVVFSRSNLVLGSRSVARFMHLTNDDRVLCVLPFSFDAGLNQFLSA 206 A ++YTSGSTG+PKG S L R + + L D VL PF FD + +FL Sbjct: 2796 AYVIYTSGSTGRPKGAGNSHRALYNRLRWMQQAYALQEGDAVLQKTPFGFDVSVWEFLWP 2855 Query: 207 LTVGATVVLID---FVHASQIEECCATHGITSITAVPGLWSKKPYLGRTAEQGKATHWQX 263 L GA +V+ +++ HG++++ VP + L G+A Sbjct: 2856 LMTGARLVMAPPGAHRDPAELAALIREHGVSTLHFVPSM------LQAFLASGQA----- 2904 Query: 264 DASRRSVRRIGNTGGHLQVELLKQLQNIFSNADVFLMYGFTE-AFRATYLDPGTVARKPH 322 A+ S+R++ +G L E Q + A + +YG TE A T+ R P Sbjct: 2905 -AACTSLRQVICSGEALPAEARDQFFALLPQAALHNLYGPTEAAIDVTHWTCQHGDRGPV 2963 Query: 323 SVGRPIPFASVAVVTEEGRLCAPDEIGELVQFGPLVTLGYHNLPEENAVKFRPLPVGLVD 382 +G+PI V+ + + GEL G + GY P A +F P G + Sbjct: 2964 PIGQPISGLRAVVLDADLNVLPCGVAGELYLGGIGLARGYLGRPGLTAERFVASPFGQGE 3023 Query: 383 ELMGPRGRHYVFDPAHATAAAWSGDLVSIDEDGDIVYRGRKDDLIKVNGFRICAGDIEDA 442 L +GDLV EDG I Y GR D +K+ G RI G+IE A Sbjct: 3024 RLYR------------------TGDLVRWREDGQIDYLGRLDHQVKIRGLRIELGEIE-A 3064 Query: 443 CVEAGLAIAIAIGMTGGEEEGVVVFGKCA-------DASTGEREIRERLRFNLPTYMIPR 495 + A + A+ + G V+ G A DA+T +++ L LP YM+P Sbjct: 3065 QLLALDEVEEAVVVAHEHGAGPVLAGYVASRRWQRQDAAT----LKQALAARLPDYMVPA 3120 Query: 496 AIFLIDDFPLTSNGKFDRQALRALALQQSEGLASA 530 + L+ PL +NGK DR+AL A L SE A A Sbjct: 3121 VLTLLPTLPLNANGKIDRKALPAPRL-HSEDFAYA 3154 Score = 95.1 bits (235), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 134/519 (25%), Positives = 195/519 (37%), Gaps = 87/519 (16%) Query: 24 LRERVNAGMENLRSLGVRSGDRIAICGPKSTDLCVLMLATWGVGAVTVPIFSGLKTKQVR 83 L RV LRSLGV GD +A+ P+ +L + +LATW GA +P+ + + + Sbjct: 4594 LERRVEHLAHALRSLGVAPGDIVALQLPRCAELVLALLATWRCGAAYLPLDTDSPVARRQ 4653 Query: 84 HILSDSEPTLVF--AGMRDFAMVAQAHGGTTHPLEHFGTQAHVTSAAPQCGDFYASAAAV 141 IL ++ L+ AG + A L + QA +AP G Sbjct: 4654 QILQAAKVHLLVMEAGQSAEGLPADCTAVAWDELANSPVQA----SAPLPGS-------- 4701 Query: 142 TEAAPALIVYTSGSTGQPKGVVFSRSNLVLGSRSVARFMHLTNDDRVLCVLPFSFDAGLN 201 A A +++TSGSTG PKGV+ + L + V + + L + D G Sbjct: 4702 RMDATAYVLFTSGSTGAPKGVLIGHAQLAAYVQGVTQALRLDACRHWALTGTVAADLGNT 4761 Query: 202 QFLSALTVGATVVLI---DFVHASQIEECCATHGITSITAVPG-----LWSKKPYLGRTA 253 AL GA + + D + + HGI ++ VP L + PYL T Sbjct: 4762 ALFGALANGACLHVASAEDMRDGERFAKFLRQHGIDALKIVPSHLDALLETPAPYLPATL 4821 Query: 254 EQGKATHWQXDASRRSVRRIGNTGGHLQVELLKQLQNIFSNADVFLMYGFTE-------- 305 G G Q L +++ + VF YG TE Sbjct: 4822 VLG-----------------GEATAPAQ---LARIRALAPQCRVFNHYGPTECTVGVLVH 4861 Query: 306 -----AFRATYLDPGTVARKPHSVGRPIPFASVAVVTEEGRLCAPDEIGELVQFGPLVTL 360 A DPG A P + +P A V V+ + R IG+L G + Sbjct: 4862 RAATEAEAEAEADPG--ASLP--LTEALPHAYVRVLDAQLRPTPAGAIGQLWLGGAQLAR 4917 Query: 361 GYHNLPEENAVKFRPLPVGLVDELMGPRGRHYVFDPAHATAAAWSGDLVSIDEDGDIVYR 420 GY + ++ +D+ P R Y SGDL + G + Sbjct: 4918 GYLDAQQDRE--------AFIDDPWLPGRRLYR-----------SGDLAVLLPQGGVRLA 4958 Query: 421 GRKDDLIKVNGFRICAGDIEDAC-VEAGLAIAIAIGMTGGEEEGVVVFGKCADASTGER- 478 GR D +KV GFRI G+IE A + G+ A+ + G C A G+ Sbjct: 4959 GRADHQVKVRGFRIEPGEIEAALRTQPGVRQALVLAAPDG------ALAACLAADHGQAL 5012 Query: 479 -EIRERLRFNLPTYMIPRAIFLIDDFPLTSNGKFDRQAL 516 R L LP M P ++ + P NGK DR+AL Sbjct: 5013 PAWRAALAEVLPAQMQPSRWLVLPELPRLGNGKVDRKAL 5051 Score = 92.4 bits (228), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 128/516 (24%), Positives = 206/516 (39%), Gaps = 76/516 (14%) Query: 24 LRERVNAGMENLRSLGVRSGDRIAICGPKSTDLCVLMLATWGVGAVTVPIFSGLKTKQVR 83 L ++ N L + G + + ++ ++ V +LA GA +P+ L ++ Sbjct: 7228 LNQQANRLAHRLLAEGAGPERIVGVAARRAPEMVVALLAVLKAGAAYLPLDPDLPPARLA 7287 Query: 84 HILSDSEPTLVFAGMRDFAMVAQAHGGTTHPLEHFGTQAHVTS-AAPQCGDFYASAAAVT 142 +++DS +L+ L +A +T+ AA + + A Sbjct: 7288 DMMADSGLSLL--------------------LTQTALRAELTAPAAVRVLELDAPGQPWA 7327 Query: 143 EAAP-------------ALIVYTSGSTGQPKGVVFSRSNLVLGSRSVARFMHLTNDDRVL 189 E P A ++YTSGSTG+PKG +L + + + VL Sbjct: 7328 EGMPDHDPGIEVAPDGLAYVIYTSGSTGKPKGAGNRHVSLHNRLAWMQEAYPIGPGNTVL 7387 Query: 190 CVLPFSFDAGLNQFLSALTVGATVVLI---DFVHASQIEECCATHGITSITAVPGLWSKK 246 PF FD + +F L GA + L + + + H + ++ VP + Sbjct: 7388 QKTPFGFDVSVWEFFWPLMTGARLALAAPDEHRDPALLAARIRRHAVDTLHFVPSML--- 7444 Query: 247 PYLGRTAEQGKATHWQXDASRRSVRRIGNTGGHLQVELLKQLQNIFSNADVFLMYGFTEA 306 QG H A S+R++ +G L EL +Q + +YG TEA Sbjct: 7445 --------QGFVAH-PDSAQCHSLRQVICSGEALPAELQEQAFARLPQIALHNLYGPTEA 7495 Query: 307 -FRATYLD--PGTVARKPHSVGRPIPFASVAVVTEEGRLCAPDEI-GELVQFGPLVTLGY 362 T+ PG + +GRPI + A V + G P I GEL G + GY Sbjct: 7496 AIDVTHWTCRPG---ERSVPIGRPISH-TAAYVLDGGLNPLPHGIAGELYLGGIGLARGY 7551 Query: 363 HNLPEENAVKFRPLPVGLVDELMGPRGRHYVFDPAHATAAAWSGDLVSIDEDGDIVYRGR 422 P +A +F + D R Y +GDLV DG+I Y GR Sbjct: 7552 LGRPGLSAERF------VADPFAADGSRMYR-----------TGDLVRRRADGEIEYLGR 7594 Query: 423 KDDLIKVNGFRICAGDIEDACV-EAGLAIAIAIGMT-GGEEEGVVVFGKCADASTGEREI 480 D +K+ G RI G+IE + + G+A A+ + + G +V + + Sbjct: 7595 TDHQVKIRGLRIELGEIEAQLLAQPGVAAAVVVALVEPGRPARLVGYAADPGGMLEAEAL 7654 Query: 481 RERLRFNLPTYMIPRAIFLIDDFPLTSNGKFDRQAL 516 R L +LP YM+P+ + L+ PL +NGK DR+AL Sbjct: 7655 RAALLQSLPDYMVPQRLILLPALPLNANGKVDRKAL 7690 Score = 73.6 bits (179), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 120/492 (24%), Positives = 183/492 (37%), Gaps = 73/492 (14%) Query: 45 RIAICGPKSTDLCVLMLATWGVGAVTVPIFSGLKTKQVRHILSDSEPTLVF-AGMRDFAM 103 R+A+ + D MLA GA VP+ L +++ L DS V G A Sbjct: 1196 RVALYAGRDIDFVCGMLAVLDTGAAYVPLDPQLPAERLAWQLQDSGARWVLHEGQSAPAW 1255 Query: 104 VAQAHGGTTHPLEHFGTQAHVTSAAPQCGDFYASAAAVTEAAPALIVYTSGSTGQPKGVV 163 +AQA G LE GT A+P + V A A ++YTSGSTG+PKGV+ Sbjct: 1256 LAQA-GVPLLSLEAAGT----APASPW------TPFPVHPAQAAYLIYTSGSTGRPKGVL 1304 Query: 164 FSRSNLVLGSRSVARFMHLTNDDRVLCVLP-FSFDAGLNQFLSALTVGATVVLIDFVHAS 222 +R L +V + L R + ++ + D G AL G + LI A Sbjct: 1305 VTRGGLANYVAAVLEKLALPAAARSMAMISTVAADLGHTTLYGALCSGRQLHLITHDLAF 1364 Query: 223 QIEECCA---THGITSITAVPGLWSKKPYLGRTAE---------QGKATHWQXDASRRSV 270 + A H + + VP + R + G+AT W Sbjct: 1365 DPDGFAAYLHEHAVDVLKIVPSHLQALLHAARPEQVLPRHTLVLGGEATGW--------- 1415 Query: 271 RRIGNTGGHLQVELLKQLQNIFSNADVFLMYGFTEAFRATYLDPGTVARKPHSV---GRP 327 LL+++ + V YG +E + A + +V G Sbjct: 1416 ------------PLLERIAALQPACRVLNHYGPSETTVGVLVQEAASASRRAAVLPVGSA 1463 Query: 328 IPFASVAVVTEEGRLCAPDEIGELVQFGPLVTLGYHNLPEENAVKFRPLPVGLVDELMGP 387 + V+ + R +GEL G + GYH A +F P L Sbjct: 1464 LVGVQAWVLDRQLRRAPVGAVGELYLGGAGLARGYHGRAALTAERFVAHPFASGQRLYR- 1522 Query: 388 RGRHYVFDPAHATAAAWSGDLVSIDEDGDIVYRGRKDDLIKVNGFRICAGDIEDACVE-A 446 SGD DG I + GR DD +K+ G R+ G++ + A Sbjct: 1523 -----------------SGDRARYLGDGSIEFLGRIDDQVKIRGHRVEPGEVRQQLLRLA 1565 Query: 447 GLAIAIAIGMTGGEEEGVVV---FGKCADASTGERE-IRERLRFNLPTYMIPRAIFLIDD 502 G+ A + + ++ + F CA + + R L +LP Y++P + + Sbjct: 1566 GVVDAAVLALPAADDAQRLQLHGFIVCAAGAVADPAGWRATLAADLPDYLLP-TLTPVPA 1624 Query: 503 FPLTSNGKFDRQ 514 PLT+NGK DRQ Sbjct: 1625 LPLTANGKLDRQ 1636 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 96.3 bits (238), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 129/537 (24%), Positives = 206/537 (38%), Gaps = 84/537 (15%) Query: 23 QLRERVNAGMENLRSLGVRSGDRIAICGPKSTDLCVLMLATWGVGAVTVPIFSGLKTKQV 82 ++ RV+A L GV+ GD++ + + + A + +G V VP + + Sbjct: 67 EINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWVPTNFRITPPEA 126 Query: 83 RHILSDSEPTLVFAGMRDFAMVAQAHGGTTHPLEHFGTQAHVTSAAPQCG--DFYA---- 136 + L S V R FA A + LEH V A + G D+ A Sbjct: 127 AY-LGQSSGACVMLYDRGFAQYVDAVKAASPALEHV-----VALADARAGELDYEALVRE 180 Query: 137 -SAAAVTEAA-----PALIVYTSGSTGQPKGVVFSRSNLV-LGSRSVARFMH-LTNDDRV 188 S AA EA P YTSG+TG PK + S + + + +A M LT+ R Sbjct: 181 GSGAAFREAEVDYDDPLWFFYTSGTTGHPKAGMLSHGQMAFVVTNHLADLMPGLTHQSRS 240 Query: 189 LCVLPFSFDAGLNQFLSALTVGATVVL----IDFVHASQIEECCATHGITSITAVPGLWS 244 L V P S AG++ ++ A+++L + A Q+ E + ++ + + Sbjct: 241 LVVAPLSHGAGVHAVINTARGAASILLSSERLVVEEAWQLVEKYRVDNLFTVPTIVKILV 300 Query: 245 KKPYLGRTAEQGKATHWQXDASRRSVRRIGNTGGHL----QVELLKQLQNIFSNADVFLM 300 + + R S++ + G + QV LK+L + Sbjct: 301 EDESVDRY-------------DHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQ-----Y 342 Query: 301 YGFTE-----AFRATYL---DPGTVARKPHSVGRPIPFASVAVVTEEGRLCAPDEIGELV 352 YG E F YL D + + G P +A++ ++G+ AP E GE+ Sbjct: 343 YGLGEVTGNITFLPPYLHFEDDAHPQARVGTCGMPRTGMQIAILNDDGQELAPFETGEIC 402 Query: 353 QFGPLVTLGYHNLPEENAVKFRPLPVGLVDELMGPRGRHYVFDPAHATAAAWSGDLVSID 412 GP V +GYHN PE NA F+ H F +GDL +D Sbjct: 403 VRGPAVFMGYHNNPEANAKAFK----------------HDWFH---------TGDLGHVD 437 Query: 413 EDGDIVYRGRKDDLIKVNGFRICAGDIEDACVEAGLAIAIAI----GMTGGEEEGVVVFG 468 +DG + GR D+ G + +IE+A + +A+ GE V+ Sbjct: 438 QDGFLYITGRSSDMYISGGSNVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVC 497 Query: 469 KCADASTGEREIRERLRFNLPTYMIPRAIFLIDDFPLTSNGKFDRQALRALALQQSE 525 K GE + L + Y PR D P + GK ++ ++AL +Q E Sbjct: 498 KAGQQVDGE-ALLAHLESRIGKYKWPRRFIYWDSMPKSGYGKIVKKQIKALLEEQGE 553 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 92.4 bits (228), Expect = 8e-20, Method: Compositional matrix adjust. Identities = 129/510 (25%), Positives = 201/510 (39%), Gaps = 66/510 (12%) Query: 35 LRSLGVRSGDRIAICGPKSTDLCVLMLATWGVGAVTVPIFSGLKTKQVRHILSDSEPTLV 94 LR+ GV GDRI I + + + LA + +GA+TVP+F + + L +S + Sbjct: 79 LRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNSGAVAL 138 Query: 95 F---AGMRDFAMVAQAHGGTTHPLEHFGTQAHVTSAAPQCGDFYASAAAVTEAA------ 145 +GM + G L PQ DF+ A Sbjct: 139 VTDASGMEKATL-----AGELPALRLVFCVESSDLVLPQTTDFWDRLHAAPAELDPVLTL 193 Query: 146 ---PALIVYTSGSTGQPKGVVFSRSNLV--LGSRSVARFMHLTNDDRVLCVLPFSFDAGL 200 PA+I+YTSG+TG+ KG + + L+ L V+ DR +++ GL Sbjct: 194 ADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFPQEGDRFWTPADWAWIGGL 253 Query: 201 -NQFLSALTVGATVVL--IDFVHASQIEECCATHGITSITAVPGLWSKKPYLGRTAEQGK 257 + L +L G VV ++ A+++ A H I ++ P + R A + Sbjct: 254 LDVLLPSLYHGVAVVARRLEKFDAAEVFGLLARHQIRNVFFPPTALK----MLRGAATVR 309 Query: 258 ATHWQXDASRRSVRRIGNTGGHLQVELLKQLQNIFSNADVFLMYGFTEAFRATYLDPGTV 317 A Q D S RSV G T G + ++ + N YG TE Sbjct: 310 A---QADFSLRSVASGGETLGDDLIAWGREALGVTINE----FYGQTECNLVVSSSSHCY 362 Query: 318 ARKPHSVGRPIPFASVAVVTEEGRLCAPDEIGELVQFGP--LVTLGYHNLPEENAVKFRP 375 S+GR +P V +V E+G++ +G + P ++ L Y E KF Sbjct: 363 PSVSGSMGRAVPGHVVQIVDEQGQVLPHGTVGNIAIRAPDPVMFLRYWRNEEATREKFAG 422 Query: 376 LPVGLVDELMGPRGRHYVFDPAHATAAAWSGDLVSIDEDGDIVYRGRKDDLIKVNGFRIC 435 D L+ +GDL S+DE G I Y GR DD+I G+RI Sbjct: 423 ------DFLL-------------------TGDLGSMDEQGYIRYLGRNDDVITSAGYRIG 457 Query: 436 AGDIEDACV-EAGLAIAIAIGMTGG-EEEGVVVFGKCADASTGER----EIRERLRFNLP 489 IE+ + + IA +G+ E V F D T + E+++ +R L Sbjct: 458 PAAIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDGVTPDEALKAELQQHVRAQLA 517 Query: 490 TYMIPRAIFLIDDFPLTSNGKFDRQALRAL 519 + PR I + P T+ GK R+ L+ + Sbjct: 518 AHEYPRLISFVAALPTTATGKIMRKTLKEM 547 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 73.2 bits (178), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 120/508 (23%), Positives = 193/508 (37%), Gaps = 52/508 (10%) Query: 35 LRSLGVRSGDRIAICGPKSTDLCVLMLATWGVGAVTVPIFSGLKTKQVRHILSDSEPTLV 94 LR G++ G +A P++ +L +L T GAV P+F+ K + H L+ S +L+ Sbjct: 83 LRQQGIQPGQIVAGMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLI 142 Query: 95 FAGMRDFAMVAQAHG--------GTTHPLEHFGTQAHVTSAAPQCGDFYASAAAVTEAAP 146 + + + + + PL G + Q DF + + A Sbjct: 143 VTNVANRDKLDEIANCPQVCTVRESNDPLRA-GDIDFRAAVDAQSDDF--APVLRSGADL 199 Query: 147 ALIVYTSGSTGQPKGVVFSRSNLVLGSRSVARFMHLTNDDRVLCVLPFSFDAGLN-QFLS 205 L++ TSG+TG KGV S L S + + L D+ + + GL + Sbjct: 200 MLMMSTSGTTGAAKGVPVPLSALQAFSVYMREAVGLLPQDKFWNMADPGWAYGLYYAIIG 259 Query: 206 ALTVGATVVLIDFVHA-SQIEECCATHGITSITAVPGLWSKKPYLGRTAEQGKATHWQXD 264 L +G + + S E GITS+ P + A Sbjct: 260 PLAIGHGITFNEAAFTVSGTYETIRRLGITSLAGAPTAYRMM---------MAAGEEAAA 310 Query: 265 ASRRSVRRIGNTGGHLQVELLKQLQNIFSNADVFLMYGFTEAFRATYLDPGTVARKPHSV 324 + + +R + + G L E+++ + + + YG TE G V H++ Sbjct: 311 SVKGRLRAVSSAGEPLNAEVVRWFASAL-DVPIHDHYGQTEL--------GMVVNNHHAL 361 Query: 325 GRPIPFASVAVVTEEGRLCAPDEIGELVQFGPLVTLGYHNLPEENAVKFRPLPVGLVDEL 384 + S R+ DE + LG N P E A+ P L Sbjct: 362 RHEVVPGSAGFAMPGYRVVVLDENHQ--------ELG-ANQPGELAIDIARSP------L 406 Query: 385 MGPRGRHYVFDPAHATAAAWSGDLVSIDEDGDIVYRGRKDDLIKVNGFRICAGDIEDACV 444 G PA A +GD V ++ +G I + GR DD+I G+RI D+E A + Sbjct: 407 YWFSGYWKQDTPAIAHGYYSTGDNVELEPNGSISFIGRSDDVITSAGYRIGPFDVESALL 466 Query: 445 E-AGLAIAIAIGMTGGEEEGVV-VFGKCADASTGEREIRERL----RFNLPTYMIPRAIF 498 E +A IG+ E +V F +D +RE+L + L + PRAI Sbjct: 467 EHPAVADVAVIGLPDPERTEIVKAFVVLSDQFKASDALREQLAQHVKRRLSAHAYPRAIE 526 Query: 499 LIDDFPLTSNGKFDRQALRALALQQSEG 526 +D P T +GK R LR + + G Sbjct: 527 FLDALPKTPSGKLQRFVLRKMEADRRAG 554 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,659,590 Number of extensions: 69617 Number of successful extensions: 284 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 261 Number of HSP's successfully gapped: 12 Length of query: 531 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 441 Effective length of database: 1,193,720 Effective search space: 526430520 Effective search space used: 526430520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)