BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0154.p (438 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 311 7e-86 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 306 2e-84 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 301 1e-82 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 290 2e-79 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 276 2e-75 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 311 bits (797), Expect = 7e-86, Method: Compositional matrix adjust. Identities = 190/420 (45%), Positives = 261/420 (62%), Gaps = 11/420 (2%) Query: 10 SDADAADKVVRKSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLM 69 S+ A + +A+ R I + ++GNALE FD I+ FAV+I KQFFP SLL+ Sbjct: 3 SNTAAPGADITHPQAR-RAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLL 61 Query: 70 TMATFGAGFVMRPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAP 129 T ATFG F MRPLG +V+GAY+DR GRK A+ML LM GT +I PSY SIG+LAP Sbjct: 62 TFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAP 121 Query: 130 LLIVTGRLLQGFSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLS 189 I G+++QGFS GGE G+STA L E A + RGF SWQ+ASQG + L+ AL G +L+ Sbjct: 122 AGIALGKMIQGFSAGGEFGSSTAFLVEHA-PHRRGFFSSWQVASQGISLLLAALFGAVLN 180 Query: 190 TFLSQQELDSWGWRLPFLFGLMLGPLGYAIRSMLHETPPIE--SRKGHA-VRLVFSRFYH 246 L+ ++L SWGWR+PF+FGL++ P G IR L E P + S K +A +R F+ Sbjct: 181 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKM 240 Query: 247 PLITGVLLMTGGTASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLS 306 L+ G + T S+++ + Y+PTY V L +P SF +AG I+ +GSP G LS Sbjct: 241 RLLIGAGSVIMATVSVYLSL-YIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALS 299 Query: 307 DHLARRKPFVIFSRLLGASLIYPAFWLLNHTAQPAF-ALVAIGTLAGCL-TLGSGASFLL 364 D + R PF+I S L L YP F L ++ P F L+ + T+ G L T+ A L Sbjct: 300 DKIG-RTPFMITSSALYIVLTYPMFVFLTNS--PGFLQLLLLQTVIGVLMTMYFAAMPAL 356 Query: 365 LVESFPQAVRATGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIA 424 L + FP A R TG SL Y+I V+IFGGFA ++TWLI+ +GD +S ++++I +++SLIA Sbjct: 357 LADIFPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIA 416 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 306 bits (784), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 178/423 (42%), Positives = 247/423 (58%), Gaps = 11/423 (2%) Query: 15 ADKVVRKS-EAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLMTMAT 73 AD +V S + + R I A +GN LE FDFT++SFFAV+I K FFP L SLL+ +AT Sbjct: 8 ADGIVSVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVAT 67 Query: 74 FGAGFVMRPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIV 133 FG GF MRP+GG+++G YADR GRK A+ + LMA GT +IG+ P+Y+SIG+ APL+IV Sbjct: 68 FGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIV 127 Query: 134 TGRLLQGFSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFLS 193 RLLQGFS GGE+G++TA L E A R + SW AS G A L+GA G ++ L Sbjct: 128 VARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLD 187 Query: 194 QQELDSWGWRLPFLFGLMLGPLGYAIRSMLHETPPIESRKGHA---VRLVFSRFYHPLIT 250 L SWGWRLPFL G+++GP+GY IR L ETP K ++ V F Sbjct: 188 AAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSA 247 Query: 251 GVLLMTGGTASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHLA 310 ++ T ++++FYMPTY V L +P + F+ + G ++V +P G L+D + Sbjct: 248 SFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIG 307 Query: 311 RRKPFVIFSRLLGASLIYPAFWLLNHTAQPAFALV---AIGTLAGCLTLGSGASFLLLVE 367 RR P + S L L YP F +N + L+ GTL T G E Sbjct: 308 RR-PLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYT---GPILAAFSE 363 Query: 368 SFPQAVRATGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIP 427 FP V +TG S+ Y++ V+IFGGFA F +TWLI +G M+PA Y+++ + +S + Sbjct: 364 LFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRF 423 Query: 428 MKE 430 ++E Sbjct: 424 VRE 426 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 301 bits (770), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 172/417 (41%), Positives = 251/417 (60%), Gaps = 13/417 (3%) Query: 25 KFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLMTMATFGAGFVMRPLG 84 K R + A VGNALE +DF ++ FF+ +I + FFPA SLL+ +ATFG GF MRP+G Sbjct: 7 KQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPVG 66 Query: 85 GLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRLLQGFSMG 144 G+++G YADR GRK AM + LM II TP+Y +IG+ AP++IV R+LQGF+ G Sbjct: 67 GVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFATG 126 Query: 145 GEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFLSQQELDSWGWRL 204 GE +STA L E+A + RG SWQ+ Q A GA G L++ LS + LDSWGWRL Sbjct: 127 GEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWRL 186 Query: 205 PFLFGLMLGPLGYAIRSMLHETPP-IES-RKGHAVRLVFSRFYHPLITGVLLMTG----G 258 PF+ GL++GP+G IR +HET +ES K +A + + GVL+ G G Sbjct: 187 PFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVING 246 Query: 259 TASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHLARRKPFVIF 318 T + ++V+ MPT+ L +P +F+ +A A+L PFAG LSD L RR +++ Sbjct: 247 TVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRT-VLLY 305 Query: 319 SRLLGASLIYPAF-WLLNHTAQPAFALVAIGTLAGCLTLGS--GASFLLLVESFPQAVRA 375 S L ++YP F W+ +A P+ + + + C+ +G+ G L E FP VR+ Sbjct: 306 SSLAFLLMVYPLFVWV---SAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRS 362 Query: 376 TGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIPMKEHS 432 TG ++ Y++ +FGGFAPF+VTWL + SG P++PA Y++ + + + A M++ S Sbjct: 363 TGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAMRDQS 419 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 290 bits (742), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 168/416 (40%), Positives = 236/416 (56%), Gaps = 7/416 (1%) Query: 22 SEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLMTMATFGAGFVMR 81 S++KF + VT GN +EMFDF +F F+A I K FFP+ ASL++T TFGAGF+MR Sbjct: 7 SQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGFLMR 66 Query: 82 PLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRLLQGF 141 PLG +++GAY DR GR+ +++ +LMA GT +I PSY +IG LAPLL++ GRLLQGF Sbjct: 67 PLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGF 126 Query: 142 SMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFLSQQELDSWG 201 S G E+G + LAE A ++GF +SWQ ASQ A +V A GYLLST L+ E+ WG Sbjct: 127 SAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWG 186 Query: 202 WRLPFLFGLMLGPLGYAIRSMLHETPPIESRK-----GHAVRLVFSRFYHPLITGVLLMT 256 WR+PF G ++ P+ + IR L ET RK G R + + +I G++L++ Sbjct: 187 WRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIEN-WKLVIAGMMLVS 245 Query: 257 GGTASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHLARRKPFV 316 T S +++ Y PT+ L + T S + G + P G LSD + RR V Sbjct: 246 MTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRPLLV 305 Query: 317 IFSRLLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLTLGSGASFLLLVESFPQAVRAT 376 F+ +L YPA L H A A L+ L+ +GA + L E P VR Sbjct: 306 TFT-VLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVRTA 364 Query: 377 GFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIPMKEHS 432 GFSL YS+ ++FGGF P + T LIE + D +P +M ++ LIA + + + Sbjct: 365 GFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRNK 420 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 276 bits (706), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 160/410 (39%), Positives = 232/410 (56%), Gaps = 7/410 (1%) Query: 20 RKSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLMTMATFGAGFV 79 +K + VT GN LEMFDF ++ F+A I K FFP+ SL++T TFGAGF+ Sbjct: 7 NAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGAGFL 66 Query: 80 MRPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRLLQ 139 MRPLG +++G+Y DR GR+ +++ ++MA GT +I P Y +IGV AP+L++TGRLLQ Sbjct: 67 MRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRLLQ 126 Query: 140 GFSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFLSQQELDS 199 GFS G E+G + LAE A ++GF +SWQ ASQ AA + AL GYL+S LS ++ Sbjct: 127 GFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQISD 186 Query: 200 WGWRLPFLFGLMLGPLGYAIRSMLHETPPIESRKGH----AVRLVFSRFYHPLITGVLLM 255 WGWR+PF G M+ P + IR L ET +RK H + ++ + ++ G++++ Sbjct: 187 WGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVVAGMMMV 246 Query: 256 TGGTASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHLARRKPF 315 T S +++ Y PT+ L + S S + G P G LSD + RR Sbjct: 247 VMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRRPVL 306 Query: 316 VIFSRLLGASLIYPAF-WLLNHTAQPAFALVAIGTLAGCLTLGSGASFLLLVESFPQAVR 374 V F+ LL YP WL++ + + + L+ +GA+ + L E P VR Sbjct: 307 VAFT-LLTLLTAYPVMSWLVSDISFKNLLITELW-LSFLYASYNGAAVVALTEVMPAHVR 364 Query: 375 ATGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIA 424 GFSL YS+ +IFGGF P V TWLIE SGD +P ++M ++ L+A Sbjct: 365 TVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLA 414 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,330,816 Number of extensions: 58689 Number of successful extensions: 360 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 328 Number of HSP's successfully gapped: 16 Length of query: 438 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 350 Effective length of database: 1,203,190 Effective search space: 421116500 Effective search space used: 421116500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)