BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0143.p (312 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxy... 399 e-112 gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herba... 140 9e-35 gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herba... 119 3e-28 >gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 296 Score = 399 bits (1024), Expect = e-112, Method: Compositional matrix adjust. Identities = 191/283 (67%), Positives = 223/283 (78%) Query: 21 VDEFSITELVNRQIAATAPQRLADIVTALTRHLHDFAREVRLSENELMAAIQFLTQVGQT 80 ++E +IT+ V + A QRL +I+T+L +HLH FAREV+L+E E A I FLT+ GQ Sbjct: 12 LNEDTITQAVLSRHAWLTDQRLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQI 71 Query: 81 CNDKRQEFILLSDVLGLSMLVTAQNNRKPQGCTESTVFGPFHVENSPEHAMGDDIANGAP 140 C+D+RQEFILLSD LGLS LV AQ N KP GCTE+TVFGPFHVE++P++ +G DIANGA Sbjct: 72 CSDQRQEFILLSDTLGLSTLVMAQQNHKPAGCTEATVFGPFHVEDAPQYPLGADIANGAK 131 Query: 141 GEPCFVRGVVRGLGGEPVANARIEVWQADEDGFYDVQYDGMDQARARATLRTGPDGAYRF 200 GEPCFV G VRG G P+A+AR+EVWQADE+GFYDVQY +DQ RAR TL T +G Y F Sbjct: 132 GEPCFVSGSVRGRNGRPLAHARMEVWQADENGFYDVQYADLDQYRARGTLFTDAEGRYHF 191 Query: 201 RSILASSYPIPHDGPVGKLLAALGRHPWRPAHLHFLIKAEGYETLITHVFRNGDHYLDSD 260 RSILA+ YPIPHDGPVG++L LGRHPWRPAHLHF I+AEGYETLITHVFR D YLDSD Sbjct: 192 RSILANPYPIPHDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYLDSD 251 Query: 261 AVFGVRQSLIADWVRHPAGPGPDGRLLTAPFYTLDFDFALNPL 303 AVFGVR +LIADW HPAG PDG PFYTLD+DF LNPL Sbjct: 252 AVFGVRSTLIADWPCHPAGTAPDGTQCEQPFYTLDYDFVLNPL 294 >gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 303 Score = 140 bits (354), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 94/297 (31%), Positives = 142/297 (47%), Gaps = 15/297 (5%) Query: 17 DTRYVDEFSITELVNRQIAATAPQRLADIVTALTRHLHDFAREVRLSENELMAAIQFLTQ 76 D + +D ++ + + A QR+ +V L R + E+ + E AA+ +LT Sbjct: 2 DKKAID--AVLQKIESTELAEGNQRVKTVVNRLVRDMFYTIEELDVQPEEFWAAVDYLTS 59 Query: 77 VGQTCNDKRQEFILLSDVLGLSMLV-----TAQNNRKPQGCTESTVFGPFHVENSPEH-A 130 G++ EF L++ LG + A+ R QG T T+ GP +V +PE Sbjct: 60 AGKSG-----EFGLIAAGLGFEHFLDLRMDEAEARRGVQGGTPRTIEGPLYVSGAPEQKG 114 Query: 131 MGDDIANGAPGEPCFVRGVVRGLGGEPVANARIEVWQADEDGFYDVQYDGMDQARARATL 190 + PG+ F++G V G+P+ANA +EVW A+ G Y R ++ Sbjct: 115 FARMDKDPQPGDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQSPFNLRCSI 174 Query: 191 RTGPDGAYRFRSILASSYPIPHDGPVGKLLAALGRHPWRPAHLHFLIKAEGYETLITHVF 250 RT G YRFRS + Y +P G +LL LGRH RPAH+HF + GY L T + Sbjct: 175 RTDEQGRYRFRSRVPVGYSVPPGGATDRLLTKLGRHGSRPAHIHFFVTVPGYRKLTTQIN 234 Query: 251 RNGDHYLDSDAVFGVRQSLIADWVRHPAGPGPDGR-LLTAPFYTLDFDFALNPLPGD 306 GD YL D F R+ L+ + ++H P + + APFY ++F+F L P D Sbjct: 235 IAGDPYLWDDFAFATREGLVPE-LKHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQAD 290 >gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 311 Score = 119 bits (298), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 81/273 (29%), Positives = 126/273 (46%), Gaps = 16/273 (5%) Query: 41 RLADIVTALTRHLHDFAREVRLSENELMAAIQFLTQVGQTCNDKRQEFILLSDVLGLSML 100 R IV + L ++ ++ +E A + ++ ++G E LLS LGL Sbjct: 29 RTKQIVHRILSDLFKTIEDLEITSDEYWAGVAYINRLGTA-----HEAGLLSPGLGLDRF 83 Query: 101 VTAQNNRKPQ-----GCTESTVFGPFHVENSPEHAMGDDIANGA--PGEPCFVRGVVRGL 153 + + + + G T T+ GP +V +P + +G+ G+ + G V G Sbjct: 84 LDMRMDAEDAALGIAGGTPRTIEGPLYVAGAPVEQGFARLDDGSDKSGQTIIMHGTVYGA 143 Query: 154 GGEPVANARIEVWQADEDGFYDVQYDGMDQAR---ARATLRTGPDGAYRFRSILASSYPI 210 G+P+ A++EVW A+ GFY +D + + R T+ T G Y+FRSI+ ++Y Sbjct: 144 DGKPMPGAQVEVWHANTKGFYS-HFDPTGEQKPFNMRRTIITDAQGRYKFRSIMPTAYGC 202 Query: 211 PHDGPVGKLLAALGRHPWRPAHLHFLIKAEGYETLITHVFRNGDHYLDSDAVFGVRQSLI 270 P GP LL ALGRH RPAH+HF + AEG+ L T + GD + D + R+ L+ Sbjct: 203 PPAGPTQGLLNALGRHGNRPAHIHFFVTAEGHRKLTTQINIEGDPLIYDDFAYATREGLV 262 Query: 271 ADWVRHPAGPGPDGRLLTAPFYTLDFDFALNPL 303 V L PF + FD L L Sbjct: 263 PPLVERTDAASIKAEGLEGPFAEIVFDIKLTSL 295 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,116,188 Number of extensions: 51196 Number of successful extensions: 135 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 3 Length of query: 312 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 227 Effective length of database: 1,217,395 Effective search space: 276348665 Effective search space used: 276348665 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)