BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0140.p (167 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313235|ref|YP_003777327.1| 2,3-dihydroxybiphenyl dioxygena... 54 5e-09 >gi|300313235|ref|YP_003777327.1| 2,3-dihydroxybiphenyl dioxygenase [Herbaspirillum seropedicae SmR1] Length = 165 Score = 54.3 bits (129), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 40/122 (32%), Positives = 59/122 (48%), Gaps = 10/122 (8%) Query: 11 HVGIHVTDMDRSRAFYRDVLKLTITDMDPVSGATFFSSRPEEEHHELLL----APGRTVK 66 H+ + V D S +Y DVL +T+T F S ++HH++ L P + V Sbjct: 23 HLALFVKDPHGSAPWYEDVLGMTVTAR--AERWVFLSF--GKKHHDIALIRVEEPSQAV- 77 Query: 67 RGEVLVQQVSFRC-NTLEEVIYFYKRLLEADAEIEQVVTHGNAIGVYFFDPDGNRCEVYW 125 RGEV +Q +EE Y LL EI ++ H G+YF DPDG+R E ++ Sbjct: 78 RGEVNMQHYGLEVEGDMEEFRRLYGMLLAKGIEIFKITDHKVGYGIYFHDPDGHRFEFFF 137 Query: 126 DT 127 +T Sbjct: 138 ET 139 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 458,173 Number of extensions: 16188 Number of successful extensions: 54 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 53 Number of HSP's successfully gapped: 1 Length of query: 167 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 88 Effective length of database: 1,245,805 Effective search space: 109630840 Effective search space used: 109630840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)