BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0129.p (221 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313173|ref|YP_003777265.1| gluconolactonase [Herbaspirillu... 89 2e-19 gi|300310377|ref|YP_003774469.1| gluconolactonase [Herbaspirillu... 79 4e-16 gi|300313737|ref|YP_003777829.1| gluconolactonase [Herbaspirillu... 67 1e-12 gi|300313307|ref|YP_003777399.1| gluconolactonase [Herbaspirillu... 65 6e-12 >gi|300313173|ref|YP_003777265.1| gluconolactonase [Herbaspirillum seropedicae SmR1] Length = 302 Score = 89.4 bits (220), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 59/198 (29%), Positives = 96/198 (48%), Gaps = 24/198 (12%) Query: 30 FNDFTVDDDGSVFIGAMGFDINTFDPSRQKPPPGKLFRVSPSGEAKLLYEGVMVPNGIGF 89 FND D G + G + ++P + G + + V+ NG+ F Sbjct: 107 FNDGRCDASGRFWCGTI------YEPRDRD---GGTLYCFERNALRDAHHAVVTSNGLAF 157 Query: 90 SPDRKRLYQSDTMTHAVWVYDVN-ADRDVSDRRVFHRFADDE-------LPDGLAVDVEG 141 S D++ +Y ++T H + +YD + A S+ R+ +F D+ PDG AVD +G Sbjct: 158 SSDQRLMYHANTPAHRINLYDYDLATGATSNCRLLRQFDSDKSAPDYGGRPDGAAVDSQG 217 Query: 142 GVWVAIAKGRGEIVRLTPEGGIDRRIKVPSTFVTSLAFGGAELTDLYVVTANNSEVES-- 199 W A+ +G G ++RL+P+G I + I++P+ T +AFGG +L LY+ T N + Sbjct: 218 AYWCAMFEG-GRVLRLSPQGEILQEIRLPARCPTMVAFGGDDLRTLYITTGRNGRSPAEL 276 Query: 200 ----RGGTVFRTRVEVPG 213 G V RV+VPG Sbjct: 277 AQYPESGCVLAVRVDVPG 294 >gi|300310377|ref|YP_003774469.1| gluconolactonase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 78.6 bits (192), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 60/199 (30%), Positives = 90/199 (45%), Gaps = 17/199 (8%) Query: 26 PIPGFNDFTVDDDGSVFIGAMGFDINTFDPSRQKPPPGKLFRVSPS-GEAKLLYEGVMVP 84 P ND D G G + D + P +R++ +L + + Sbjct: 96 PSTRLNDGRCDRQGRFVFGTLNEDAG-------RAPIASFYRLNTDLTLERLSLPSIAIS 148 Query: 85 NGIGFSPDRKRLYQSDTMTHAVWVYDVN-ADRDVSDRRVFHRFADDELPDGLAVDVEGGV 143 N I FS D K +Y D+M + V D + A V +RVF A PDG +D EG + Sbjct: 149 NSICFSLDGKLMYHCDSMAGKIMVCDYDTASGAVGGQRVFADVAQPGGPDGSTIDAEGYL 208 Query: 144 WVAIAKGRGEIVRLTPEGGIDRRIKVPSTFVTSLAFGGAELTDLYVVTAN------NSEV 197 W A G +VR P+G IDR + +P++ + +AFGGA+ LYV TA+ Sbjct: 209 WNA-QWGGARVVRYAPDGRIDRVVAIPTSQPSCVAFGGAQFDTLYVTTAHEGMSAEQRAA 267 Query: 198 ESRGGTVFRTRV-EVPGLP 215 + + G +F + +V GLP Sbjct: 268 DPQAGALFAVALADVRGLP 286 >gi|300313737|ref|YP_003777829.1| gluconolactonase [Herbaspirillum seropedicae SmR1] Length = 306 Score = 67.0 bits (162), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 53/190 (27%), Positives = 85/190 (44%), Gaps = 17/190 (8%) Query: 29 GFNDFTVDDDGSVFIGAMGFDINTFDPSRQKPPPGKLFRVSPSGEAKLLYEGVMVPNGIG 88 G ND G ++ G + + P G+++R++P G+ L + PNGI Sbjct: 128 GVNDLVFGPKGEIYFTDQG-------QTGLQDPTGRVYRLAPDGQLTCLINTIPSPNGIV 180 Query: 89 FSPDRKRLYQSDTMTHAVWVYDVNADRDVSDRRVFHRFADDEL-PDGLAVDVEGGVWVAI 147 + P L + T +W ++ + V VF PDGLA+D E +++A Sbjct: 181 YDPALNHLLVAVTRAQQIWRIPLHHNGVVGKVGVFANLHGGMAGPDGLALDAESNLYIA- 239 Query: 148 AKGRGEIVRLTPEGGIDRRIKVPSTF-VTSLAFGGAELTDLYVVTANNSEVESRGGTVFR 206 G G I RL+ RI+ + T+LAFGG + L++ ES+ G++ R Sbjct: 240 HTGFGSIWRLSKFAEPLLRIRSSAGISTTNLAFGGPDGKSLFI-------TESQTGSILR 292 Query: 207 TRVEVPGLPI 216 V V GLP+ Sbjct: 293 ADVPVAGLPM 302 >gi|300313307|ref|YP_003777399.1| gluconolactonase [Herbaspirillum seropedicae SmR1] Length = 305 Score = 64.7 bits (156), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 53/198 (26%), Positives = 91/198 (45%), Gaps = 16/198 (8%) Query: 3 GTTLALVDEAGGVRDIFSRWNGSPIPGFNDFTVDDDGSVFIGAMGFDINT-FDPSRQKPP 61 G + + G + + S W G + ND V DGS++ + IN+ ++ + + Sbjct: 94 GRAVVRTEHDGSITTLASHWQGKRLNSPNDVVVKSDGSIWFTDPDYGINSDYEGYQAQSE 153 Query: 62 PG--KLFRV-SPSGEAKLLYEGVMVPNGIGFSPDRKRLYQSDTMT-------HAVWVYDV 111 G ++R+ SGE ++ + PNG+ FS D +RLY +DT H + V+DV Sbjct: 154 IGACNVYRIDGDSGEISIVASDLERPNGLAFSTDERRLYIADTGLTHRAGGPHHIRVFDV 213 Query: 112 NADRDVSDRRVFHRFADDELPDGLAVDVEGGVWVAIAKGRGEIVRLTPEGGIDRRIKVPS 171 + VF D L DG +D +G VW + G + P+G + +I +P Sbjct: 214 VDGISLKVGEVFATI-DPGLADGFRLDAQGNVWTSAGDG---VHCYAPDGTLLGKILIPE 269 Query: 172 TFVTSLAFGGAELTDLYV 189 V+++ FGG L++ Sbjct: 270 V-VSNVVFGGPRGNRLFI 286 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.140 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 826,143 Number of extensions: 38032 Number of successful extensions: 93 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 87 Number of HSP's successfully gapped: 4 Length of query: 221 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 139 Effective length of database: 1,231,600 Effective search space: 171192400 Effective search space used: 171192400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)