BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0126.p (362 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312181|ref|YP_003776273.1| coenzyme F420-dependent N5,N10-... 92 6e-20 >gi|300312181|ref|YP_003776273.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 92.0 bits (227), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 62/202 (30%), Positives = 104/202 (51%), Gaps = 8/202 (3%) Query: 12 GIFDHIEKSSGSANIGDLYRERIAFVKHAESLGFWGYHLAEHHCTPLGVVPSPALFLASL 71 G+F ++E G+A D + + AE+LG+ +AEHH + + + + LA + Sbjct: 9 GVFLNVENPHGNAR--DALLQTVDHAVVAEALGYHDVWVAEHHYSSFAIGSALMVLLAQI 66 Query: 72 ASHTSRIRLGPMVFLLPFYNPLRLMLEICMLDNMSDGRFEFGIGRG---VHPYEHAYYNI 128 A+ TSRIRLG LL +PLR+ ++ LD +S+GR EFG+ RG Y HA + Sbjct: 67 AARTSRIRLGTGASLLALNDPLRVAEDVATLDLLSNGRMEFGVARGGPFPEQYRHAGLSG 126 Query: 129 PTLQSTEIFEEALDVILKGFTVDQLKHKGTHFRFDGVPVADMRPVQKPYPGLWYGAVKES 188 Q+ EALDVI + + +G F +D V + +P+Q+P P +W ++ E Sbjct: 127 AD-QARARMIEALDVIQRAWQGVPFSTEGRFFHYDRVQL-QPQPLQRPIP-VWLASMSED 183 Query: 189 SVIYAAKRGLNICTNGPVEMNK 210 S+ AA++ + ++ + Sbjct: 184 SLDLAARQDYGLMATPSADLEQ 205 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,135,976 Number of extensions: 47660 Number of successful extensions: 97 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 97 Number of HSP's successfully gapped: 1 Length of query: 362 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 276 Effective length of database: 1,212,660 Effective search space: 334694160 Effective search space used: 334694160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)