BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0114.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 179 6e-46 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 154 2e-38 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 152 4e-38 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 138 9e-34 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 130 2e-31 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 179 bits (453), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 121/261 (46%), Positives = 162/261 (62%), Gaps = 8/261 (3%) Query: 21 GQWREGA-AASLLQDHDPYTGQLLTEIREASLGDLDAAYAAAAQAQQAWAAVLPSERAAL 79 GQW A A++L +P TG+ LT++ AS D+ A+ A AQ AW A+ P ERA + Sbjct: 19 GQWVAAAQGATVL---EPATGRPLTQVGLASPDDVSASVELAIAAQAAWVAMPPRERADI 75 Query: 80 FLRAAAIMDQRHAEIVDWLIRESGSTRIKAEIEWAAVKSGMLAATAMPARVHGRILPIDI 139 F RAA + Q E+ + RE+G K E E + A MP + G++LP Sbjct: 76 FRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQAQGQLLP-ST 134 Query: 140 AGRESRVYRRPLGVVGVISPWNWPMHLSNRSIAPALAVGNAVVVKPADDTPVTGGLLLAK 199 GR S R PLGV+GVISP+N+P+ L+ R++APALA GNAVVVKP TPV+GG +LA+ Sbjct: 135 PGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRTPVSGGFMLAR 194 Query: 200 IYEEAGLPPGLLNVVIGAVSEIGDAFTLHPTPKFISFTGSTRVGRHIGALAMTGPTIKRV 259 I+E+AGLP GLL+V+ G +E G+A P I+FTGS VGR IG LA G +K++ Sbjct: 195 IFEQAGLPAGLLHVLPGG-AETGEALVTAPGVPMIAFTGSPAVGRRIGELA--GRHLKKL 251 Query: 260 GLELGGNAPCVVLDDADLERA 280 LELGG V+L+DADL+ A Sbjct: 252 SLELGGANALVILEDADLDVA 272 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 154 bits (388), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 89/263 (33%), Positives = 149/263 (56%), Gaps = 3/263 (1%) Query: 18 YIDGQWREGAAASLLQDHDPYTGQLLTEIREASLGDLDAAYAAAAQAQQAWAAVLPSERA 77 +I+G+W A+ + +P T +++ +I A DLD A AA++ + W + ER+ Sbjct: 8 FINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFERS 67 Query: 78 ALFLRAAAIMDQRHAEIVDWLIRESGSTRIKAEIEWAAVKSGMLAATAMPARVHGRILPI 137 + RAA ++ +R E+ + E G +A++E + R +GR++P Sbjct: 68 KIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVPA 127 Query: 138 DIAGRESRVYRRPLGVVGVISPWNWPMHLSNRSIAPALAVGNAVVVKPADDTPVTGGLLL 197 + G V + P+G V +PWN+P++ R ++ ALA G +++VK ++TP + L+ Sbjct: 128 RVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELI 187 Query: 198 AKIYEEAGLPPGLLNVVIGAVSEIGDAFTLHPTPKFISFTGSTRVGRHIGALAMTGPTIK 257 + Y +AG+P G++N+V G +EI + HP K ISFTGST VG+ + ALA G +K Sbjct: 188 -RAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALA--GQHMK 244 Query: 258 RVGLELGGNAPCVVLDDADLERA 280 R +ELGG+AP +V DDAD++ A Sbjct: 245 RATMELGGHAPAMVFDDADVDVA 267 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 152 bits (385), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 110/293 (37%), Positives = 154/293 (52%), Gaps = 16/293 (5%) Query: 4 NETLPHYRQGFTGQYIDGQWREGAAASLLQDHDPYTGQLLTEIREASLGDLDAAYAAAAQ 63 N L H+ YIDG+W+ + P T + + EI + D+D A AA + Sbjct: 12 NARLDHF-------YIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARR 64 Query: 64 AQQAWAAVLPSERAALFLRAAAIMDQRHAEIVDWLIRESGSTRIKAEIEWAAVKSGMLAA 123 A AWAA P ERAAL R A++ +R L E G A I +A LAA Sbjct: 65 AIPAWAATSPLERAALLGRVHALLLERSELFAQALTLEMG-----APISYARTAHVPLAA 119 Query: 124 TAM-PARVHGRILPIDIAGRESRVYRRPLGVVGVISPWNWPMHLSNRSIAPALAVGNAVV 182 + AR + P + + R P+GV +I+PWNWP++ + PALA G VV Sbjct: 120 EHLRVARDNLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVV 179 Query: 183 VKPADDTPVTGGLLLAKIYEEAGLPPGLLNVVIGAVSEIGDAFTLHPTPKFISFTGSTRV 242 +KP++ +P++ LL A+I EAG+P G+ N+V G+ +E+G A + HP IS TGSTR Sbjct: 180 LKPSELSPLS-ALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRA 238 Query: 243 GRHIGALAMTGPTIKRVGLELGGNAPCVVLDDADLERAVDGAILVASCTRGRS 295 G + A T+KRV ELGG +P +VL DADLERA+ + A G+S Sbjct: 239 GVLVAQAAAV--TVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQS 289 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 138 bits (348), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 95/270 (35%), Positives = 138/270 (51%), Gaps = 7/270 (2%) Query: 18 YIDGQWREGAAASLLQDHDPYTGQLLTEIREASLGDLDAAYAAAAQAQQAWAAVLPSERA 77 +++GQW + L H+P G+LL + + A AA A AW ER+ Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 78 ALFLRAAAIMDQRHAEIVDWLIRESGS--TRIKAEIEWAAVKSGMLAATAMPARVHGRIL 135 A+ R +M ++ + E G + EI +AA A R +G + Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEG--KRTYGDTI 132 Query: 136 PIDIAGRESRVYRRPLGVVGVISPWNWPMHLSNRSIAPALAVGNAVVVKPADDTPVTGGL 195 P V + +GV I+PWN+P + R PALA G +V+KPA+ TP + L Sbjct: 133 PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFS-AL 191 Query: 196 LLAKIYEEAGLPPGLLNVVIGAVSEIGDAFTLHPTPKFISFTGSTRVGRHIGALAMTGPT 255 LA + E AG+P G+ +VV G IG T +P + ISFTGST VG+ + + + + Sbjct: 192 ALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLL--MQQSASS 249 Query: 256 IKRVGLELGGNAPCVVLDDADLERAVDGAI 285 IK++ LELGGNAP +V DDADL+ AV+GAI Sbjct: 250 IKKLSLELGGNAPFIVFDDADLDAAVEGAI 279 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 130 bits (328), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 88/244 (36%), Positives = 121/244 (49%), Gaps = 5/244 (2%) Query: 36 DPYTGQLLTEIREASLGDLDAAYAAAAQAQQ-AWAAVLPSERAALFLRAAAIMDQRHAEI 94 DP GQ I + D+D A AA A + AW + +ER+ L ++ A + E+ Sbjct: 23 DPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLSAAERSRLLMKLAYKLQDHQEEL 82 Query: 95 VDWLIRESGSTRIKAEIEWAAVKSGMLAATAMPARVHGRILPIDIAGRESRVYRRPLGVV 154 R+ G +A + AA+ ++HG +P AG R P GV Sbjct: 83 AQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHGDTIPYQ-AGYTVLTLREPHGVT 141 Query: 155 GVISPWNWPMHLSNRSIAPALAVGNAVVVKPADDTPVTGGLLLAKIYEEAGLPPGLLNVV 214 G I PWN+PM + R + ALA GN VVKPA+D ++ L +A++ E G P G LN+V Sbjct: 142 GHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLS-ILRVAELAAEVGFPEGALNIV 200 Query: 215 IGAVSEIGDAFTLHPTPKFISFTGSTRVGRHIGALAMTGPTIKRVGLELGGNAPCVVLDD 274 G E GD HP ISFTGS VG+ + A T V LELGG +P +V +D Sbjct: 201 TGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHHT--PVTLELGGKSPQIVFED 258 Query: 275 ADLE 278 AD+E Sbjct: 259 ADME 262 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.135 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,362,918 Number of extensions: 53819 Number of successful extensions: 276 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 228 Number of HSP's successfully gapped: 19 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)