BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0106.p (432 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 864 0.0 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 684 0.0 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 312 4e-86 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 301 8e-83 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 293 2e-80 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 864 bits (2232), Expect = 0.0, Method: Compositional matrix adjust. Identities = 431/432 (99%), Positives = 431/432 (99%) Query: 1 MTTTTTNAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGT 60 MTTTTTNAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGT Sbjct: 1 MTTTTTNAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGT 60 Query: 61 FGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVL 120 FGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVL Sbjct: 61 FGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVL 120 Query: 121 TGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLS 180 TGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLS Sbjct: 121 TGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLS 180 Query: 181 PVQISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVV 240 PVQISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVV Sbjct: 181 PVQISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVV 240 Query: 241 AGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRV 300 AGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRV Sbjct: 241 AGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRV 300 Query: 301 GRRPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMP 360 GRRPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMP Sbjct: 301 GRRPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMP 360 Query: 361 GHVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLYR 420 HVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLYR Sbjct: 361 AHVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLYR 420 Query: 421 GKSAAATVSPTA 432 GKSAAATVSPTA Sbjct: 421 GKSAAATVSPTA 432 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 684 bits (1766), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/420 (78%), Positives = 371/420 (88%) Query: 4 TTTNAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGA 63 TT+ A SK STVLRVTSGNF+EMFDFFL+GFYA++ISK FFPSDNEF SL+LTF TFGA Sbjct: 2 TTSTASQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGA 61 Query: 64 GFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGR 123 GFLMRPLGAIILG+Y+DRVGRRQGLIVTLA+MA+GT+LIAFVP YATIG AP+LVL GR Sbjct: 62 GFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGR 121 Query: 124 LLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQ 183 LLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQ AI+ +A IGYL+S SL+P + Sbjct: 122 LLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAE 181 Query: 184 ISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVVAGM 243 +S WGWRIPFFIGC+IVP +F+IRRSLQET+EF+ RK PS G+I S+ +NWK+V+AGM Sbjct: 182 VSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIAGM 241 Query: 244 MMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRR 303 M+V MTTVSFYLITVYTPTFGKNVLKLS SLIVT CVGLSNF WLPIMGALSDRVGRR Sbjct: 242 MLVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRR 301 Query: 304 PVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPGHV 363 P+LV FT+LT+LTAYP ++WLV D SF +LI ELWLSFLYASYNGA VVALTEVMP V Sbjct: 302 PLLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADV 361 Query: 364 RTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLYRGKS 423 RT GFSLAYSLATA+FGGFTP ++T LIE + DKAAPG WMS AAVCGL+ATL+LYR K+ Sbjct: 362 RTAGFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRNKA 421 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 312 bits (799), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 166/423 (39%), Positives = 245/423 (57%), Gaps = 3/423 (0%) Query: 11 SKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGAGFLMRPL 70 +K V+ GN LE +DF +YGF+++ I++ FFP+DNE+ SL++ TFG GF MRP+ Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 Query: 71 GAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRLLQGFSA 130 G ++LG Y DR GR+ + V + +M + +IAF P YATIG+AAPI+++ R+LQGF+ Sbjct: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 Query: 131 GVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQISDWGWR 190 G E + +L E A +G Y SWQ Q A+ A +G LV+++LSP + WGWR Sbjct: 126 GGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWR 185 Query: 191 IPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEI--MSSIAQNWKIVVAGMMMVVM 248 +PF +G +I P IRR + ETD+FL P+ I + + N + V+ M V+ Sbjct: 186 LPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVIN 245 Query: 249 TTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRRPVLVA 308 TV+FY++ V PTF L L + +V +P GALSDR+GRR VL+ Sbjct: 246 GTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLLY 305 Query: 309 FTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPGHVRTVGF 368 +L LL YP+ W+ + S LL+ ++ L + + G AL+E P VR+ G Sbjct: 306 SSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGL 365 Query: 369 SLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLYRGKSAAATV 428 ++AY+LA +FGGF P + TWL +TSG AP +++ FAA G+ A + R +S AA Sbjct: 366 AVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAM-RDQSPAAKN 424 Query: 429 SPT 431 T Sbjct: 425 RQT 427 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 301 bits (770), Expect = 8e-83, Method: Compositional matrix adjust. Identities = 165/413 (39%), Positives = 247/413 (59%), Gaps = 7/413 (1%) Query: 4 TTTNAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGA 63 T A+ + +S+ + GN LE FD +YG +A I+K FFP+ ++ VSL+LTF TFG Sbjct: 13 THPQARRAIISSSI----GNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGV 68 Query: 64 GFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGR 123 F MRPLGA++LG+Y DR GR+ L++++ +M +GT +IAF+P YA+IG+ AP + G+ Sbjct: 69 SFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGK 128 Query: 124 LLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQ 183 ++QGFSAG E G + +L E A P +GF+ SWQ ASQ +++ +AL G +++ L+P Q Sbjct: 129 MIQGFSAGGEFGSSTAFLVEHA-PHRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQ 187 Query: 184 ISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVVAGM 243 ++ WGWR+PF G +I PA IRR L E EF + + + A ++ G Sbjct: 188 LASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEF-KESSEKTNAPLRDTFAHQKMRLLIGA 246 Query: 244 MMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRR 303 V+M TVS YL ++Y PT+ L L A S GL F P++GALSD++GR Sbjct: 247 GSVIMATVSVYL-SLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRT 305 Query: 304 PVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPGHV 363 P ++ + L ++ YP+ +L + F LL+ + + L Y A L ++ P Sbjct: 306 PFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVAT 365 Query: 364 RTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATL 416 R G SLAY++A IFGGF L+ TWLI+ +GDK + Y++ F AV L+A+L Sbjct: 366 RGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASL 418 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 293 bits (749), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 161/409 (39%), Positives = 233/409 (56%), Gaps = 3/409 (0%) Query: 8 AKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGAGFLM 67 + + ++ GN LE FDF +Y F+A I+K FFP+ N+ SL+L TFG GF M Sbjct: 15 SNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFM 74 Query: 68 RPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRLLQG 127 RP+G I+LG Y DRVGR+ L VT+ +MA+GT +I P Y +IG+ AP++++ RLLQG Sbjct: 75 RPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQG 134 Query: 128 FSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQISDW 187 FSAG E+G + +L E A + +Y SW AS A++ A +G V+ SL ++ W Sbjct: 135 FSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASW 194 Query: 188 GWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNW-KIVVAGMMMV 246 GWR+PF +G +I P + IR L+ET FL K + + +N+ + A MV Sbjct: 195 GWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTD--SPLKEVIRNFPRETSASFSMV 252 Query: 247 VMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRRPVL 306 ++ TV Y++ Y PT+ VLKL + I GL PI+G L+DR+GRRP+L Sbjct: 253 ILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPLL 312 Query: 307 VAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPGHVRTV 366 L L+ AYP+ +++ S S LLI +L L A+Y G + A +E+ P V + Sbjct: 313 SGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLST 372 Query: 367 GFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLAT 415 G S+AY+LA IFGGF TWLI +G AP +++ AA + T Sbjct: 373 GLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGT 421 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,272,040 Number of extensions: 50664 Number of successful extensions: 340 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 309 Number of HSP's successfully gapped: 14 Length of query: 432 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 344 Effective length of database: 1,203,190 Effective search space: 413897360 Effective search space used: 413897360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)