BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0103.p (596 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3... 1177 0.0 gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3... 294 1e-80 gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspi... 162 9e-41 gi|300310786|ref|YP_003774878.1| dihydrolipoamide acetyltransfer... 140 4e-34 gi|300312272|ref|YP_003776364.1| dihydrolipoamide succinyltransf... 64 3e-11 >gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 1177 bits (3045), Expect = 0.0, Method: Compositional matrix adjust. Identities = 591/596 (99%), Positives = 593/596 (99%) Query: 1 MSLSEVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEIK 60 MSLSEVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKE+K Sbjct: 1 MSLSEVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEVK 60 Query: 61 VKVGDKVAEGSLLLLVEAAGQATAPPAPAAAPAPAAAAAPAPAAAPAAAPTAAPTAASFG 120 VKVGDKVAEGSLLLLVEAAGQA A PAPAA PAPAAAAAPAPAAAPAAAPTAAPTAASFG Sbjct: 61 VKVGDKVAEGSLLLLVEAAGQAAAAPAPAAPPAPAAAAAPAPAAAPAAAPTAAPTAASFG 120 Query: 121 GSADVQCDVMVLGGGPGGYSAAFRAADLGLNTVIVEREATLGGVCLNVGCIPSKALLHVA 180 GSADVQCDVMVLGGGPGGYSAAFRAADLGLNTVIVEREATLGGVCLNVGCIPSKALLHVA Sbjct: 121 GSADVQCDVMVLGGGPGGYSAAFRAADLGLNTVIVEREATLGGVCLNVGCIPSKALLHVA 180 Query: 181 AVIDETAAMASHGVTFGKPEIDIDKLRAYKDKVIGTMTGGLAGMAKARKVQVVNGDGQFA 240 AVIDETAAMASHGVTFGKP+IDIDKLRAYKDKVIGTMTGGLAGMAKARKVQVVNGDGQFA Sbjct: 181 AVIDETAAMASHGVTFGKPQIDIDKLRAYKDKVIGTMTGGLAGMAKARKVQVVNGDGQFA 240 Query: 241 GPNHIEVTAADGSKKVVQFKHAIIAAGSSVVNLPFVPQDPRIVDSTGALELRQVPKRMLV 300 GPNHIEVTAADGSKKVVQFKHAIIAAGSSVVNLPFVPQDPRIVDSTGALELRQVPKRMLV Sbjct: 241 GPNHIEVTAADGSKKVVQFKHAIIAAGSSVVNLPFVPQDPRIVDSTGALELRQVPKRMLV 300 Query: 301 IGGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFDNVMVKTKTV 360 IGGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFDNVMVKTKTV Sbjct: 301 IGGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFDNVMVKTKTV 360 Query: 361 AVEALPEGIKVTFEAAEAGATAPEPQLYDLVLVAVGRSPNGKKLSADKAGVIVSDRGFIA 420 AVEALPEGIKVTFEAAEAGATAPEPQLYDLVLVAVGRSPNGKKLSADKAGVIVSDRGFIA Sbjct: 361 AVEALPEGIKVTFEAAEAGATAPEPQLYDLVLVAVGRSPNGKKLSADKAGVIVSDRGFIA 420 Query: 421 VDKQMRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAIAGEKSFFDASVIPSVAYTDPE 480 VDKQMRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAIAGEKSFFDASVIPSVAYTDPE Sbjct: 421 VDKQMRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAIAGEKSFFDASVIPSVAYTDPE 480 Query: 481 VAWVGVTEDEAKAKGIKIEKGHFPWAASGRAVANGRSEGFTKLLFDAETHRIIGGGIVGT 540 VAWVGVTEDEAKAKGIKIEKGHFPWAASGRAVANGRSEGFTKLLFDAETHRIIGGGIVGT Sbjct: 481 VAWVGVTEDEAKAKGIKIEKGHFPWAASGRAVANGRSEGFTKLLFDAETHRIIGGGIVGT 540 Query: 541 HAGDMIGEVALAIEMGADAVDIGKTIHPHPTLGESLGMAAEVAEGHCTDLPPQRKK 596 HAGDMIGEVALAIEMGADAVDIGKTIHPHPTLGESLGMAAEVAEGHCTDLPPQRKK Sbjct: 541 HAGDMIGEVALAIEMGADAVDIGKTIHPHPTLGESLGMAAEVAEGHCTDLPPQRKK 596 >gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 294 bits (753), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 173/452 (38%), Positives = 258/452 (57%), Gaps = 13/452 (2%) Query: 139 YSAAFRAADLGLNTVIVEREATL------GGVCLNVGCIPSKALLHVAAVIDETA-AMAS 191 Y AA RAA LG NT ++ GG C NVGCIPSKALL + + + A Sbjct: 17 YIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKALLQSSEHYEHASHGFAE 76 Query: 192 HGVTFGKPEIDIDKLRAYKDKVIGTMTGGLAGMAKARKVQVVNGDGQF--AGPNHIEVTA 249 HG+ ++++K+ K+ V+ G+ + K KV +G G F N E+ Sbjct: 77 HGIEVKGLGLNLEKMLGRKNTVVKQNNDGILYLFKKNKVSFFHGRGSFVKGDANGYEIKV 136 Query: 250 ADGSKKVVQFKHAIIAAGSSVVNLPFVPQDPR-IVDSTGALELRQVPKRMLVIGGGIIGL 308 A +++ + KH I+A GS+ LP D + I+ +TGAL + +VPK++ VIG G+IGL Sbjct: 137 AGAAEETITAKHVIVATGSNARALPGAEFDEKLILSNTGALSITEVPKKLGVIGAGVIGL 196 Query: 309 EMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFDNVMVKTKTVAVEALPEG 368 EM +V+ LGA + V+E + + D + K QK K+ V + K A++A Sbjct: 197 EMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLTKQGLAVNLGVKIGAIKAGKNS 256 Query: 369 IKVTFEAAEAGATAPEPQLYDLVLVAVGRSPNGKKLSADKAGVIVSDRGFIAVDKQMRTN 428 + V + A+ A E +D ++V++GR+PN L+A+ G+ + +RGFIAVD +TN Sbjct: 257 VSVEYTDAKGDAHTGE---FDKLIVSIGRTPNTIGLNAEGVGLKLDERGFIAVDGDCKTN 313 Query: 429 VPHIFAIGDLVGQPMLAHKAVHEGHVAAEAIAGEKSFFDASVIPSVAYTDPEVAWVGVTE 488 +P+++A+GD+V PMLAHKA EG AE IAG+ + + IP V YT PE+AWVG TE Sbjct: 314 LPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVIYTSPEIAWVGKTE 373 Query: 489 DEAKAKGIKIEKGHFPWAASGRAVANGRSEGFTKLLFDAETHRIIGGGIVGTHAGDMIGE 548 + KA+G++ + G FP+ A+GRA A G + G K L DA+T I+G IVG A ++I E Sbjct: 374 QQLKAEGVQYKAGTFPFLANGRARALGDTSGMVKFLADAKTDEILGVHIVGPMASELISE 433 Query: 549 VALAIEMGADAVDIGKTIHPHPTLGESLGMAA 580 +A+E A + DI + H HP+L E+ AA Sbjct: 434 AVVAMEFRASSEDIARICHAHPSLSEATKEAA 465 >gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 162 bits (409), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 135/459 (29%), Positives = 216/459 (47%), Gaps = 25/459 (5%) Query: 128 DVMVLGGGPGGYSAAFRAADLGLNTVIVEREATLGGVCLNVGCIPSKALLHVAAVIDETA 187 D+ +GGG GG AA A+ G + E+ LGG C+N+GCIP K + + A +E A Sbjct: 7 DLFTIGGGSGGVRAARFASQAGARVGLAEK-GDLGGTCVNLGCIPKKLMSYSAHYHEEFA 65 Query: 188 AMASHGVTF-GKPEIDIDKLRAYKDKVIGTMTGGLAGMAKARKVQVVNGDGQFAGPNHIE 246 A +G T G+P D L A KD+ I + + KV + G FA Sbjct: 66 DAAGYGWTLNGQPSFDWSALMANKDREIAHLNDIYLRLLDNAKVSLHRG---FAKVEDAH 122 Query: 247 VTAADGSKKVVQFKHAIIAAGSSVVNLPFVPQDPRIVDSTGALELRQVPKRMLVIGGGII 306 DG + +H ++A G + P +P + S L+ +P+R +V+GGG I Sbjct: 123 TVNVDGQRFTA--RHILVATGGRP-DKPAIPGAELGITSDDFFHLKALPQRAVVLGGGYI 179 Query: 307 GLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFDNVMVKTKTVAVEALP 366 +E+A++ + LG + +V + L++ D D+ K+ ++ A+EA Sbjct: 180 AVELASILNGLGCEVTLVYRRERLLRNMDADLGIHLADEMAKKGIRIVFNASIEAIEAEQ 239 Query: 367 EGIKVTFEAAEAGA-----TAPEPQLYDLVLVAVGRSPNGKKLSADKAGVIVSDRGFIAV 421 G EA+EA T E VL A GR+ N L +AGV + G I V Sbjct: 240 AG-----EASEASVKTVRLTNGEALSAQCVLFATGRTANTAGLGLQEAGVKLKANGAIEV 294 Query: 422 DKQMRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAI--AGEKSFFDASVIPSVAYTDP 479 ++ ++VP I A+GD++ + L A+ E + GE+ A+ IP+ ++ P Sbjct: 295 NQDFESSVPSILAVGDVIDRVALTPVALAEAMAVVSRLFGKGERGMSYAN-IPTAVFSHP 353 Query: 480 EVAWVGVTEDEAKAK--GIKIEKGHFPWAASGRAVANGRSEGFTKLLFDAETHRIIGGGI 537 V VG++E+EA+ + ++I K F + ++ F KL+ DA T R++G + Sbjct: 354 NVGTVGLSEEEARQQFGELRIFKTDFKPLKN--TLSRNTERTFMKLVVDARTDRVLGVHM 411 Query: 538 VGTHAGDMIGEVALAIEMGADAVDIGKTIHPHPTLGESL 576 VG AG++I A+A++ GA TI HPT E Sbjct: 412 VGGDAGEVIQGFAVALQCGATKAQFDTTIGIHPTSAEEF 450 >gi|300310786|ref|YP_003774878.1| dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex [Herbaspirillum seropedicae SmR1] Length = 554 Score = 140 bits (352), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 74/80 (92%), Positives = 76/80 (95%) Query: 1 MSLSEVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEIK 60 MS EVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEIK Sbjct: 1 MSQVEVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEIK 60 Query: 61 VKVGDKVAEGSLLLLVEAAG 80 VKVGDKVAEGSLL++VE G Sbjct: 61 VKVGDKVAEGSLLVIVEGEG 80 Score = 117 bits (292), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 56/73 (76%), Positives = 69/73 (94%) Query: 5 EVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEIKVKVG 64 E++VPDIGDFKEVEVIEVMVKVGDT+K +QSL+TVESDKASMEIPSS AGV+KE+KVK+G Sbjct: 121 EIEVPDIGDFKEVEVIEVMVKVGDTVKAEQSLLTVESDKASMEIPSSHAGVIKELKVKLG 180 Query: 65 DKVAEGSLLLLVE 77 DKV++GS++ +E Sbjct: 181 DKVSKGSIIATIE 193 >gi|300312272|ref|YP_003776364.1| dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 63.9 bits (154), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 49/143 (34%), Positives = 72/143 (50%), Gaps = 6/143 (4%) Query: 1 MSLSEVKVPDIGD-FKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEI 59 M+ EVKVP + + E +++ KVG+ + D++LI +E+DK +E+PS AGV+ +I Sbjct: 1 MAQIEVKVPQLSESVAEATLLQWHKKVGEPVSRDENLIDIETDKVVLELPSPDAGVITQI 60 Query: 60 KVKVGDKVAEGSLLLLVEA-AGQATAPPAPAAAPAPAAAAAPAPAAAPAAAP---TAAPT 115 G V G ++ +++ A AP AAPAP A P P AAP A A P Sbjct: 61 IKADGATVVAGEVIAILDTDASAQVAPTEVKAAPAPQATNEPTPVAAPELASKGDVAMPA 120 Query: 116 AASFGGSADVQCDVMVLGGGPGG 138 AA ++ V G G G Sbjct: 121 AAKLLADNNLSTS-QVTGTGKDG 142 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,720,156 Number of extensions: 72415 Number of successful extensions: 371 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 359 Number of HSP's successfully gapped: 7 Length of query: 596 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 505 Effective length of database: 1,188,985 Effective search space: 600437425 Effective search space used: 600437425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)