BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0088.p (477 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 970 0.0 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 474 e-135 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 373 e-104 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 354 7e-99 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 276 2e-75 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 970 bits (2507), Expect = 0.0, Method: Compositional matrix adjust. Identities = 476/477 (99%), Positives = 476/477 (99%) Query: 1 MYKDVQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRN 60 MYKDVQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRN Sbjct: 1 MYKDVQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRN 60 Query: 61 MSAFERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRT 120 MSAFERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRT Sbjct: 61 MSAFERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRT 120 Query: 121 YGRLGPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETP 180 YGRL PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETP Sbjct: 121 YGRLVPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETP 180 Query: 181 ASPAELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAG 240 ASPAELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAG Sbjct: 181 ASPAELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAG 240 Query: 241 QHMKRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFV 300 QHMKRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFV Sbjct: 241 QHMKRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFV 300 Query: 301 DKFVTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGY 360 DKFVTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGY Sbjct: 301 DKFVTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGY 360 Query: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK Sbjct: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 Query: 421 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVTQLDV 477 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVTQLDV Sbjct: 421 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVTQLDV 477 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 474 bits (1219), Expect = e-135, Method: Compositional matrix adjust. Identities = 246/475 (51%), Positives = 347/475 (73%) Query: 1 MYKDVQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRN 60 MY D+QL+I+G + R +V +PAT +V+G++ A+RAD++ A++AA + F+ WR Sbjct: 1 MYPDLQLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQ 60 Query: 61 MSAFERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRT 120 +S ERS I+RRAA L RERA+ +A +TM+ GKPLA+A E + AA+ +DW AEE RR Sbjct: 61 VSPLERSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRL 120 Query: 121 YGRLGPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETP 180 YGR+ AR P Q+V++EPVG AA +PWNFP Q ++K++AA+AAGC++++K P E+P Sbjct: 121 YGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 Query: 181 ASPAELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAG 240 A+ + + + DAGVPAGV N+V GV +EIS++LI P+++ ISFTGS PVG +LAA A Sbjct: 181 AAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAA 240 Query: 241 QHMKRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFV 300 HMKR +MELGGHAP +VFDDA+V+ AA++LA K RNAGQVCVSP+RF VQKG++ FV Sbjct: 241 LHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFV 300 Query: 301 DKFVTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGY 360 F Q+KVG G E G+ MGP+ + RR A++ LIDDA++QG++L TGG R+G +G+ Sbjct: 301 TAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQRGH 360 Query: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 F+ PT++T +P AR M EEPFGP+A + F +E I AN LP GLA+Y F +S T Sbjct: 361 FYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDTA 420 Query: 421 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVTQL 475 H ++ +++GM+ INH G+A PELPFGGI +SG+GSEGG E E+++ TK+++Q+ Sbjct: 421 HRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMISQI 475 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 373 bits (957), Expect = e-104, Method: Compositional matrix adjust. Identities = 201/469 (42%), Positives = 287/469 (61%), Gaps = 2/469 (0%) Query: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 Q F+NG+W + G + V NPA E++G + A+ RA+EAA+ + W+ +A E Sbjct: 13 QAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKE 72 Query: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLG 125 RS I+RR +L+ +++A +MT EQGKPLAEA+ E AA I+WFAEE +RTYG Sbjct: 73 RSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTI 132 Query: 126 PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAE 185 P+ P +VIKE +G AA TPWNFP + RK ALAAGC +++K E TP S Sbjct: 133 PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALA 192 Query: 186 LIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKR 245 L AG+PAGV ++V G P I + +P+++KISFTGST VGK L + +K+ Sbjct: 193 LAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKK 252 Query: 246 ATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVT 305 ++ELGG+AP +VFDDAD+D A + +K+RNAGQ CV R VQ GV++AF K V Sbjct: 253 LSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVE 312 Query: 306 YAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPT 365 ++ KVG G E G+T GP+ N++ + +E + DAV++GA++ GGKR FF+PT Sbjct: 313 AVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPT 372 Query: 366 VLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAA 425 VL ++ + EE FGP+A + F+T +EV+ AN +GLA+Y +++ +A Sbjct: 373 VLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAE 432 Query: 426 RVESGMLTINHLGLALPEL-PFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 +ESGM+ IN GL E+ PFGG+K SG G EG I+ Y+ K + Sbjct: 433 GLESGMVGIN-TGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 480 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 354 bits (909), Expect = 7e-99, Method: Compositional matrix adjust. Identities = 208/474 (43%), Positives = 288/474 (60%), Gaps = 7/474 (1%) Query: 8 FINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFERS 67 I +W +A GR +DV +PAT +V + AD A+EAA F WR A +R+ Sbjct: 17 LIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFAAWRATPAKQRA 76 Query: 68 KIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLGPA 127 I++R DLL +++ RL++ EQGKPLAEAK E AA ++WF EEA R G + PA Sbjct: 77 GIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDIIPA 136 Query: 128 RVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELI 187 V G M +KEPVG VAA TPWNFP + RK++ ALAAGC+++ K E+TP + L+ Sbjct: 137 PVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTSLALV 196 Query: 188 RAYADAGVPAGVINLVYGV---PAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMK 244 R +AGVP GVIN+V E+ + + ++KISFTGST VGK LA + +K Sbjct: 197 RLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHSADTLK 256 Query: 245 RATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFV 304 + ++ELGG+AP +VFDDADVD A + AKFRN GQ CVSP R VQ+ V++AFVDK Sbjct: 257 KLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYDAFVDKLG 316 Query: 305 TYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGY---- 360 LKVG + +GP+ N R I ++ + DA+++GA++ TGGKR+ G+ Sbjct: 317 ARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKRLQGPGFGSDN 376 Query: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 ++ PTVL ++ + EE FGP+A I F T DEVI AN P+GLAAY ++ Sbjct: 377 YYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLAAYFYSTDVRRI 436 Query: 421 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVTQ 474 H + +ESG++ +N LA PFGG+K+SGYG EG ++ Y++TK V Q Sbjct: 437 HRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDYLHTKYVCQ 490 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 276 bits (707), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 165/472 (34%), Positives = 251/472 (53%), Gaps = 12/472 (2%) Query: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 +I+G+W V+ PATEE + +IA + AD+D+A++AA + W S E Sbjct: 17 HFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLE 76 Query: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLG 125 R+ ++ R LL ER+E A+ +T+E G P++ A+ + A AE R L Sbjct: 77 RAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLA------AEHLRVARDNLA 130 Query: 126 --PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASP 183 P P +++EP+G A TPWN+P+ Q+ K+ ALAAGC++++K E +P S Sbjct: 131 DYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSA 190 Query: 184 AELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHM 243 +AGVPAGV NLV G AE+ L HP + IS TGST G +A A + Sbjct: 191 LLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTV 250 Query: 244 KRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKF 303 KR ELGG +P +V DAD++ A A RN GQ C +PTR +V + + Sbjct: 251 KRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELA 310 Query: 304 VTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGN---KGY 360 + Q+KVG+ +A T GP+AN+ + ++ +I+ + GA+L GG + +GY Sbjct: 311 LATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGY 370 Query: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 + +PT+ + + EE FGP+ I ++T++E I AN YGL A+ + Sbjct: 371 YARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQV 430 Query: 421 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLV 472 A+AAR++SG + +N+ P+ PFGG K SG G E G E +E Y+ K + Sbjct: 431 RAVAARIQSGQVHLNYPAWD-PQAPFGGYKQSGNGREYGVEGMEEYMEVKAI 481 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,412,362 Number of extensions: 59666 Number of successful extensions: 265 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 21 Length of query: 477 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 388 Effective length of database: 1,198,455 Effective search space: 465000540 Effective search space used: 465000540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)