BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0070.p (479 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspi... 966 0.0 gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD prot... 496 e-141 gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspi... 72 9e-14 gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE prot... 61 2e-10 >gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 486 Score = 966 bits (2496), Expect = 0.0, Method: Compositional matrix adjust. Identities = 478/479 (99%), Positives = 478/479 (99%) Query: 1 MGALQVARDVLLTPFGLDESKLLKALGSMFTHKVDYADLYFQFTKSEGWSLEEGIVKTGS 60 MGALQVARDVLLTPFGLDESKLLKALGSMFTHKVDYADLYFQFTKSEGWSLEEGIVKTGS Sbjct: 8 MGALQVARDVLLTPFGLDESKLLKALGSMFTHKVDYADLYFQFTKSEGWSLEEGIVKTGS 67 Query: 61 FSIDQGVGVRAVSGDKTAFSYSDEISESALMDAAAATRTIARQGSGKIKVAGAMTPGGGR 120 FSIDQGVGVRAVSGDKTAFSYSDEISESALMDAAAATRTIARQGSGKIKVAGAM PGGGR Sbjct: 68 FSIDQGVGVRAVSGDKTAFSYSDEISESALMDAAAATRTIARQGSGKIKVAGAMIPGGGR 127 Query: 121 ALYLPHDPLTSLDATAKVQLLERVERIARAKDPRVKQVMASLSGEYDVVLVARADGVLAA 180 ALYLPHDPLTSLDATAKVQLLERVERIARAKDPRVKQVMASLSGEYDVVLVARADGVLAA Sbjct: 128 ALYLPHDPLTSLDATAKVQLLERVERIARAKDPRVKQVMASLSGEYDVVLVARADGVLAA 187 Query: 181 DIRPLVRVSVTVIAEQNGRREMGSSGGGGRYDYGYFSDVLLEQYASEAVATALVNLESRP 240 DIRPLVRVSVTVIAEQNGRREMGSSGGGGRYDYGYFSDVLLEQYASEAVATALVNLESRP Sbjct: 188 DIRPLVRVSVTVIAEQNGRREMGSSGGGGRYDYGYFSDVLLEQYASEAVATALVNLESRP 247 Query: 241 APAGPMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTI 300 APAGPMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTI Sbjct: 248 APAGPMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTI 307 Query: 301 ADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPR 360 ADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPR Sbjct: 308 ADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPR 367 Query: 361 MTNTYMLGGDRDPAEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPV 420 MTNTYMLGGDRDPAEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPV Sbjct: 368 MTNTYMLGGDRDPAEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPV 427 Query: 421 KGATLIGNGPDVLNRVSMIGNDMRLDSGVGVCGKEGQSVPVGVGQPTLRLDGVTVGGTA 479 KGATLIGNGPDVLNRVSMIGNDMRLDSGVGVCGKEGQSVPVGVGQPTLRLDGVTVGGTA Sbjct: 428 KGATLIGNGPDVLNRVSMIGNDMRLDSGVGVCGKEGQSVPVGVGQPTLRLDGVTVGGTA 486 >gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 496 bits (1276), Expect = e-141, Method: Compositional matrix adjust. Identities = 261/477 (54%), Positives = 328/477 (68%), Gaps = 8/477 (1%) Query: 7 ARDVLLTPFGLDESKLLKALGSMFTHKVDYADLYFQFTKSEGWSLEEGIVKTGSFSIDQG 66 AR LL GLDES+LL + DYADLY + + +E W LE G V +++ QG Sbjct: 29 ARWGLLAASGLDESQLLAGVSRALRKGGDYADLYLKSSVAESWHLERGKVTRSNYNQSQG 88 Query: 67 VGVRAVSGDKTAFSYSDEISESALMDAAAATRTIARQGSGKIKVAGA----MTPGGGRAL 122 G+R V G+++ + +D + + AA +A G V+ A P G Sbjct: 89 FGLRLVEGEQSVLTSADRFDATTIGKAA----DLAADAVGHAPVSPAGLVLKLPKRGPTY 144 Query: 123 YLPHDPLTSLDATAKVQLLERVERIARAKDPRVKQVMASLSGEYDVVLVARADGVLAADI 182 Y DPL SLDA K+ LLE V+R RA+ P V +VMASLS D+V +AR DG D+ Sbjct: 145 YTLDDPLPSLDAAHKIALLESVDRACRARSPLVVEVMASLSISLDLVFLARNDGYCGGDV 204 Query: 183 RPLVRVSVTVIAEQNGRREMGSSGGGGRYDYGYFSDVLLEQYASEAVATALVNLESRPAP 242 RPLVR+ V V +NGRRE S GGR F +E A+ AV AL+ LE+R AP Sbjct: 205 RPLVRLDVAVQVVRNGRREGASRTLGGRVGLATFDHAAIESVAALAVDAALIKLEARAAP 264 Query: 243 AGPMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTIAD 302 AG MTVV+GPGW G++LHEA+GHGLEGDFNR+GSS F+GRIG+RVAA GVT+VDDGT+ Sbjct: 265 AGTMTVVIGPGWNGVMLHEAVGHGLEGDFNRRGSSAFAGRIGQRVAAPGVTIVDDGTVPG 324 Query: 303 RRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPRMT 362 RGSLNIDDEG P QCTTLIEDGIL Y+QD++NARLM + TGN RRES+ LPMPRMT Sbjct: 325 SRGSLNIDDEGTPGQCTTLIEDGILVSYMQDSLNARLMGVANTGNGRRESYDVLPMPRMT 384 Query: 363 NTYMLGGDRDPAEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPVKG 422 NTYM G DPAEI+ASVK G+Y + GGQVDI++G+F+F+ASEA++IE+G++ PVKG Sbjct: 385 NTYMRAGGVDPAEIIASVKQGIYIAHLEGGQVDISSGRFMFAASEAFLIENGRLGAPVKG 444 Query: 423 ATLIGNGPDVLNRVSMIGNDMRLDSGVGVCGKEGQSVPVGVGQPTLRLDGVTVGGTA 479 AT++GNGP L V +IGND+ +++ GVCGK GQSVPVGVGQPTLR+DG+TVGGTA Sbjct: 445 ATVLGNGPQSLKNVKLIGNDLEINTASGVCGKAGQSVPVGVGQPTLRIDGMTVGGTA 501 >gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 454 Score = 72.4 bits (176), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 108/446 (24%), Positives = 183/446 (41%), Gaps = 33/446 (7%) Query: 13 TPFGLDESKLLKALGSMFTHKVDY--ADLYFQFTKSEGWSL--EEGIVKTGSFSIDQGVG 68 TPF + +L + + + D +D ++ G S+ +G ++T + D+G+G Sbjct: 4 TPFTHSQDQLKQLAQDVLRYARDKGASDAAVDISEGSGLSVAVRKGKIETIEQNKDKGIG 63 Query: 69 VRAVSGD----KTAFSYSDEISESALMDAAAATRTIARQGSGKIKVAG----AMTPGGGR 120 V G+ + + + + S+ AL + A IAR S AG M R Sbjct: 64 VTVFLGEGRQVRRGNASTSDFSQQALKETVEAAYNIARFTSID-DCAGLPDAEMLEMAPR 122 Query: 121 ALYLPHDPLTSLDATAKVQLLERVERIARAKDPRVKQVM-ASLSGEYDVVLVARADGVLA 179 L L P ++ A V L +R E A DPR+ A + ++ + A + G + Sbjct: 123 DLKL-FSPW-NISAEEAVALAQRCETAALETDPRISNSEGAGVYVQHSHFIAANSRGFMG 180 Query: 180 ADIRPLVR--VSVTVIAEQNGRREMGSSGGGGRYDYGYFSDVLLEQYASEAVATALVNLE 237 P R +SV IA + + R + +YA+E AL L Sbjct: 181 G--YPFSRHTISVAPIAGKGASMQRDDWYSSKRDPKKLAQPEAIGRYAAE---RALARLN 235 Query: 238 SRPAPAGPMTVVL-GPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVD 296 +R V+ P G+L A + G + S+ +G++V + + +++ Sbjct: 236 ARKLSTRKCPVLFEAPLAAGLL--GAFVQAVSGGALYRKSTFLLDSLGQQVFPEHIQILE 293 Query: 297 DGTIADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHL 356 D + GS D+EG T ++ +G+++GY T +AR + M TGN+ L Sbjct: 294 DPHVIGGVGSAPFDEEGVRTLKREVVRNGVVQGYFLSTYSARKLGMQTTGNSGGSHNLTL 353 Query: 357 PMPRMTNTYMLGGDRDPAEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKI 416 T G +L + GL G V+ G + AS Y +E+G I Sbjct: 354 RSELTTRADTFKG------MLKKMGTGLLVTELMGQGVNYVTGDYSRGAS-GYWVENGVI 406 Query: 417 TYPVKGATLIGNGPDVLNRVSMIGND 442 YPV+ T+ GN D+L ++ +GND Sbjct: 407 QYPVEEITIAGNMKDMLRQIVAVGND 432 >gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 61.2 bits (147), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 76/312 (24%), Positives = 126/312 (40%), Gaps = 12/312 (3%) Query: 138 VQLLERVERIARAKDPRVKQV-MASLSGEYDVVLVARADGVLAADIRPLVRVSVTVIAEQ 196 V L+ R+ER A A V Q+ A L ++A ++G L + +S T +A + Sbjct: 141 VALVARIERAALAVSSAVPQIEQAVLFSSRTRRVLANSEGFLRSSASTRHSLSCTAVAIR 200 Query: 197 NGRREMGSSGGGGRYDYGYFSDVLLEQYASEAVATALVNLESRPAPAGPMTVVLGPGWPG 256 +G +E+ S R D E + A AL L +R V+ P Sbjct: 201 DGAKEI-ESWWDARRDPAQLPSP--ESLGACAGRRALARLGARQVATQTCPVLFEPQAAA 257 Query: 257 ILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTIADRRGSLNIDDEGNPT 316 LL+E + + SS +G+ V A + + DD + GS + D G Sbjct: 258 SLLNEFV-QAISARPLYMHSSFLRDALGQEVFAAHMNISDDPLLKGGMGSRSYDGNGAAL 316 Query: 317 QCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPRMTNTYMLGGDRDPAEI 376 + TLI G L+GY AR + + + + P M ++ D + Sbjct: 317 RPRTLIAGGRLQGYYLGAYGARRLGLPLAEHGAG------PSNVMVSSTTSAASGDLEAM 370 Query: 377 LASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPVKGATLIGNGPDVLNRV 436 + + GL G V++ G F + S + +E+G+I YPV G T+ N ++ + Sbjct: 371 IRRMGTGLVISGLNGQGVNLMTGDFSRACS-GFWVENGQIAYPVAGITVASNLREMFAGI 429 Query: 437 SMIGNDMRLDSG 448 +GND +G Sbjct: 430 VAVGNDRLTQNG 441 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,497,236 Number of extensions: 66303 Number of successful extensions: 226 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 223 Number of HSP's successfully gapped: 4 Length of query: 479 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 390 Effective length of database: 1,198,455 Effective search space: 467397450 Effective search space used: 467397450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)