BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0060.p (611 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310745|ref|YP_003774837.1| amidase family protein [Herbasp... 1206 0.0 gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 342 3e-95 gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 115 8e-27 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 112 7e-26 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 100 5e-22 >gi|300310745|ref|YP_003774837.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 611 Score = 1206 bits (3120), Expect = 0.0, Method: Compositional matrix adjust. Identities = 600/611 (98%), Positives = 604/611 (98%) Query: 1 MKPSQTPQQVAAPATIAATLAAYRNGSSTPGKTVASLLAQIEQADHEEVWISRVPAKQLK 60 MKP+ PQQVAAP TIA+TLAAYRNGSSTPGKTVASLLA IEQAD EEVWISRVPAKQLK Sbjct: 1 MKPAHIPQQVAAPVTIASTLAAYRNGSSTPGKTVASLLALIEQADREEVWISRVPAKQLK 60 Query: 61 QQAAALDLMWQIKGEAVLESMPLFGIPFAVKDNIDVAGMPTTAACPEFSRVAETSATVVQ 120 QQAAALDLMWQIKGE VLESMPLFGIPFAVKDNIDVAGMPTTAACPEFSRVAETSATVVQ Sbjct: 61 QQAAALDLMWQIKGETVLESMPLFGIPFAVKDNIDVAGMPTTAACPEFSRVAETSATVVQ 120 Query: 121 RLQAAGALLVGKTNLDQFATGLVGVRSPYGAVRNAIAPDYVSGGSSSGSAVAVALGQVMF 180 RLQAAGALLVGKTNLDQFATGLVGVRSPYGAVRNAIAPDYVSGGSSSGSAVAVALGQVMF Sbjct: 121 RLQAAGALLVGKTNLDQFATGLVGVRSPYGAVRNAIAPDYVSGGSSSGSAVAVALGQVMF 180 Query: 181 ALGTDTAGSGRIPAAFNHLVGLKPSRGLISARGLVPACRTLDCISIFAHDVADAWHVLHS 240 ALGTDTAGSGRIPAAFNHLVGLKPSRGLISA+GLVPACRTLDCISIFAHDVADAWHVLHS Sbjct: 181 ALGTDTAGSGRIPAAFNHLVGLKPSRGLISAQGLVPACRTLDCISIFAHDVADAWHVLHS 240 Query: 241 AAGYDAADAYARKLPMLGLKRRGYRIAIPAATEFYGDLAAQDAWDGTLARIAELPGVTLA 300 AAGYDAADAYARKLPMLGLKRRGYRIAIPAATEFYGDLAAQDAWD TLARIAELPGVTLA Sbjct: 241 AAGYDAADAYARKLPMLGLKRRGYRIAIPAATEFYGDLAAQDAWDRTLARIAELPGVTLA 300 Query: 301 RIPFAPFADAARLLYQGPWVAERRAALGDFFVEQPQAVHPVVREITAQAADYDAVAAFQG 360 RIPFAPFADAARLLYQGPWVAERRAALGDFFV+QPQAVHPVVREITAQAADYDAVAAFQG Sbjct: 301 RIPFAPFADAARLLYQGPWVAERRAALGDFFVKQPQAVHPVVREITAQAADYDAVAAFQG 360 Query: 361 QYRLAELRRQAEQLLEGADLMLVPTTTGMPTIAEVQADPVRRNSQLGYYTNFVNLMDMSA 420 QYRLAELRRQAEQLLEGADLMLVPTTTGMPTIAEVQADPVRRNSQLGYYTNFVNLMDMSA Sbjct: 361 QYRLAELRRQAEQLLEGADLMLVPTTTGMPTIAEVQADPVRRNSQLGYYTNFVNLMDMSA 420 Query: 421 LAIPGLWRNDGLPAGVTLIGPAGADHLLAEAGARLQHQLGGAAQADAVASAPLPFTEETI 480 LAIPGLWRNDGLPAGVTLIGPAGADHLLAEAGARLQHQLGGAAQADAVASAPLPFTEETI Sbjct: 421 LAIPGLWRNDGLPAGVTLIGPAGADHLLAEAGARLQHQLGGAAQADAVASAPLPFTEETI 480 Query: 481 RLCVVGAHLSGQPLNWQLLEAGARRLASTRTAPRYRLYALANSAPPKPGLARTAGADGAA 540 RLCVVGAHLSGQPLNWQLLEAGARRLASTRTAPRYRLYALANSAPPKPGLARTAGADGAA Sbjct: 481 RLCVVGAHLSGQPLNWQLLEAGARRLASTRTAPRYRLYALANSAPPKPGLARTAGADGAA 540 Query: 541 IAVEVWEMPLRHVGAFVAAIPAPLGIGTVELEDGSSVKGFICEPAGIADATDITHHGGWI 600 IAVEVWEMPLRHVGAFVAAIPAPLGIGTVELEDGSSVKGFICEPAGIADATDITHHGGWI Sbjct: 541 IAVEVWEMPLRHVGAFVAAIPAPLGIGTVELEDGSSVKGFICEPAGIADATDITHHGGWI 600 Query: 601 NYLNALNKAPQ 611 NYLNALNKAPQ Sbjct: 601 NYLNALNKAPQ 611 >gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 471 Score = 342 bits (878), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 208/470 (44%), Positives = 284/470 (60%), Gaps = 48/470 (10%) Query: 16 IAATLAAYRNGSSTPGKTVASLLAQIEQADHEEVWISRVPAKQLKQQAAALD-------- 67 I A AAYRNGS P R ++L++ AAAL+ Sbjct: 9 IDALTAAYRNGSLDP----------------------RTVMQRLRESAAALNPTYRLYIH 46 Query: 68 LMWQIKGEAVLE--------SMPLFGIPFAVKDNIDVAGMPTTAACPEFSRVAETSATVV 119 L+ Q + E L+ S+PL+G+PF++KDNID+AG+PTTAACP FS V SAT+V Sbjct: 47 LLSQAETEPYLQALEQQSPHSLPLYGVPFSIKDNIDLAGVPTTAACPAFSYVPRESATLV 106 Query: 120 QRLQAAGALLVGKTNLDQFATGLVGVRSPYGAVRNAIAPDYVSGGSSSGSAVAVALGQVM 179 +L A GA+ VGK NLDQFATGL G RSPYGA RN++ PDY SGGSSSGS++AVALG Sbjct: 107 AQLIALGAIPVGKCNLDQFATGLNGTRSPYGACRNSVHPDYPSGGSSSGSSLAVALGLSS 166 Query: 180 FALGTDTAGSGRIPAAFNHLVGLKPSRGLISARGLVPACRTLDCISIFAHDVADAWHVL- 238 FALGTDTAGSGR+PAA N+LVGLK ++GL+S G+VPACRTLDC++ F A+A H+L Sbjct: 167 FALGTDTAGSGRVPAALNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLA 226 Query: 239 --HSAAGYDAADAYAR-KLPM-----LGLKRRGYRIAIPAATEFYGDLAAQDAWDGTLAR 290 + D D Y+R LP +G R +P EF G + + LA+ Sbjct: 227 LCTQSGQPDERDEYSRANLPHNDASAFQPPPQGLRFGVPRQLEFLGCSESPSLFVEALAQ 286 Query: 291 IAELPGVTLARIPFAPFADAARLLYQGPWVAERRAALGDFFVEQPQAVHPVVREITAQAA 350 + + GV + I F+PF AARLLY+GPWVAER + + E+P + PV+R++ +A Sbjct: 287 MEAIGGVAVP-IDFSPFLAAARLLYEGPWVAERYSTIEALLQERPDDILPVIRDVLRKAP 345 Query: 351 DYDAVAAFQGQYRLAELRRQAEQLLEGADLMLVPTTTGMPTIAEVQADPVRRNSQLGYYT 410 Y AV F+ QYRL +L+ + +++L D ++ P T+ E++ PV+RNS LG+YT Sbjct: 346 QYSAVDTFRAQYRLQQLKAECDRILATVDCVVTPAYPRPVTLEELREQPVQRNSDLGWYT 405 Query: 411 NFVNLMDMSALAIPGLWRNDGLPAGVTLIGPAGADHLLAEAGARLQHQLG 460 NF+N++D +A+A P + +GLP G+T+ G A +D L LQ G Sbjct: 406 NFMNMLDYAAVATPMNFMKNGLPWGITVFGRAFSDQALLGVADALQRAHG 455 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 115 bits (289), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 137/455 (30%), Positives = 196/455 (43%), Gaps = 30/455 (6%) Query: 13 PATIAATLAAYRNGSSTPGKTVASLLAQIEQADHEEVWI-SRVPAKQLKQQAAALDLMWQ 71 P+T++ A G +T + LA+I E + +RV A+Q + A A D + Sbjct: 2 PSTLSRLAADLAAGHTTSLQLTEQALARIADPAGEGARVFTRVYAEQARAAARASDTLRA 61 Query: 72 IKGEAVLESMPLFGIPFAVKDNIDVAGMPTTAACPEF--SRVAETSATVVQRLQAAGALL 129 + L PL G+P +VKD DVAG T A VA A VVQ L AGA+L Sbjct: 62 AR----LCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAIL 117 Query: 130 VGKTNLDQFATGLVGVRSPYGAVRNA----IAPDYVSGGSSSGSAVAVALGQVMFALGTD 185 +GKTN+ +FA +G+ YG +NA I + GGSSSG+A++V G A+G+D Sbjct: 118 IGKTNMTEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSD 177 Query: 186 TAGSGRIPAAFNHLVGLKPSRGLISARGLVPACRTLDCISIFAHDVADAWHVLHSAAGYD 245 T GS RIP+A N L G KP+ +S +G++P LD I A V A D Sbjct: 178 TGGSVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSV-------QCCAIMD 230 Query: 246 AADAYARKLPMLGLKRRGYRIAIPAATEFYG--DLAAQDAWDGTLARIAELPGVTLARIP 303 A A + +L RG R+ P G D Q A + A +P Sbjct: 231 AVLAGEDYVAVLAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVP 290 Query: 304 FAPFADAARLLYQGPWVAERRAALGDFFVEQPQAVHP--VVREITAQAADYDAVAAFQGQ 361 F A + +G + A A + + A + VV I + D A Sbjct: 291 --AFNALAAINAKGGFTAAEAYAWHRALIAENAAGYDQRVVSRIL-RGKDMSAADLLDVL 347 Query: 362 YRLAELRRQAEQLLEGADLMLVPTTTGM-PTIAEVQADP---VRRNSQLGYYTNFVNLMD 417 + Q E L G D +++PT + P IAE+QA N ++ +N +D Sbjct: 348 HARPRWIAQVEAQLAGYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLD 407 Query: 418 MSALAIPGLWRNDGLPAGVTLIGPAGADHLLAEAG 452 AL++P P G++L G D L G Sbjct: 408 GCALSLP-CHAPGSAPVGLSLAACGGQDKRLLSIG 441 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 112 bits (281), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 133/461 (28%), Positives = 201/461 (43%), Gaps = 60/461 (13%) Query: 32 KTVASLLAQIEQADHEEVWISRVPAKQLKQQAAALDLMWQIKGEAVLESMPLFGIPFAVK 91 + V +L+A+ E ++ R P +QL+ QA LD + G PL GIP A+K Sbjct: 24 EYVDALIAETEAQWDLNCYLQRDP-EQLRSQARMLD---EQGGHG-----PLSGIPIAIK 74 Query: 92 DNIDVAGMPTTAACPEF-SRVAETSATVVQRLQAAGALLVGKTNLDQFATGLVGVRSPYG 150 DNIDVAG+ +TA + V + ATVV+ L AGAL GK N+ + A G+ +G Sbjct: 75 DNIDVAGIASTAGTAALRNNVPQRHATVVEYLFGAGALHAGKANMHELAFGITNNNGVFG 134 Query: 151 AVRNAIAPDYVSGGSSSGSAVAVALGQVMFALGTDTAGSGRIPAAFNHLVGLKPSRGLIS 210 RN + ++GGSS G +A A+GTDT GS RIPAA +VGL+P+ S Sbjct: 135 PCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCGVVGLRPTSRRYS 194 Query: 211 ARGLVPACRTLDCISIFAHDVADAWHVLHSAAG-YDAADAYARKLPMLGLKRRGYRIAIP 269 G+VP T D A VAD + AG D+A P+L L R+ + Sbjct: 195 QHGVVPISHTRDTPGPMARSVADVALLDQIMAGSRDSA-------PLLALPPSQLRLGVL 247 Query: 270 AATEFYG-DLAAQDAWDGTLARIAELPGVTLARI---PFAPFADAARLLYQGPWVAERRA 325 + G + + + L ++ E GV L + AP + AA Sbjct: 248 RNPSWQGLEEEVEANANAALMQLRE-AGVELVDVDLPTLAPLSHAAGFAI---------- 296 Query: 326 ALGDFFVEQPQAVH-----PVVREITAQAADYDAVAAFQGQ---------YRLAELRRQA 371 L +F ++ P + V++ + A+ D Q YRLA +R+ Sbjct: 297 VLHEFLIDLPAYLQASDARDVIKRVLAEVGSPDVARIIQATLDQPVPEHAYRLALEQRRT 356 Query: 372 EQLL-------EGADLMLVPTTT------GMPTIAEVQADPVRRNSQLGYYTNFVNLMDM 418 Q L +G D ++ PTT G ++ + V + ++ + Sbjct: 357 MQALYRDCFRSQGVDALIFPTTALTARPLGEDDTVALRGERVPTFPAFARNADGASIAGL 416 Query: 419 SALAIPGLWRNDGLPAGVTLIGPAGADHLLAEAGARLQHQL 459 +++IP GLP G+ L GP G+D L + A L+ L Sbjct: 417 PSISIPVGLSEYGLPVGLELDGPEGSDRRLLQVAASLETVL 457 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 100 bits (248), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 69/169 (40%), Positives = 92/169 (54%), Gaps = 1/169 (0%) Query: 82 PLFGIPFAVKDNIDVAGMPTTAACPEFSRV-AETSATVVQRLQAAGALLVGKTNLDQFAT 140 PL GIP VKDN D MPTT + + A+ A V+RL+ AGA+++GKT + + A Sbjct: 118 PLHGIPILVKDNYDTVDMPTTGGTLALATLQAQADAFQVKRLREAGAVILGKTTMHELAA 177 Query: 141 GLVGVRSPYGAVRNAIAPDYVSGGSSSGSAVAVALGQVMFALGTDTAGSGRIPAAFNHLV 200 G+ V S G RN P GGSS G+ AVA +G+DT GS RIPAA +L Sbjct: 178 GVTTVSSLTGFTRNPYDPRRAPGGSSGGTGAAVAASFAAAGMGSDTCGSIRIPAAHQNLF 237 Query: 201 GLKPSRGLISARGLVPACRTLDCISIFAHDVADAWHVLHSAAGYDAADA 249 GL+ +RGL S G++P T D + A V D +L + G D D+ Sbjct: 238 GLRTTRGLASRSGVMPLSSTQDVAAPLARSVEDLAIMLDATVGSDPQDS 286 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,773,671 Number of extensions: 71179 Number of successful extensions: 291 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 278 Number of HSP's successfully gapped: 11 Length of query: 611 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 520 Effective length of database: 1,188,985 Effective search space: 618272200 Effective search space used: 618272200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)