BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0054.p (227 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310740|ref|YP_003774832.1| isochorismatase family protein ... 460 e-131 gi|300310742|ref|YP_003774834.1| cysteine hydrolase falmily prot... 213 8e-57 gi|300310658|ref|YP_003774750.1| 2,3-dihydro-2,3-dihydroxybenzoa... 71 8e-14 gi|300313872|ref|YP_003777964.1| 2,3-dihydro-2,3-dihydroxybenzoa... 56 2e-09 >gi|300310740|ref|YP_003774832.1| isochorismatase family protein [Herbaspirillum seropedicae SmR1] Length = 227 Score = 460 bits (1183), Expect = e-131, Method: Compositional matrix adjust. Identities = 224/227 (98%), Positives = 224/227 (98%) Query: 1 MIRIDAFPYPYQFHPRSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLALA 60 MIRIDAFPYPYQFHPRSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLALA Sbjct: 1 MIRIDAFPYPYQFHPRSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLALA 60 Query: 61 RQAQMLVVHTRESHLPDLSDCPRAKRLRGNPTLGIGDVGPMGRILVRGEPGNQILPQLAP 120 RQ QMLVVHTRESHLPDLSDCPR KRLRGNPTLGIGDVGPMGRILVRGEPGNQILPQL P Sbjct: 61 RQTQMLVVHTRESHLPDLSDCPRTKRLRGNPTLGIGDVGPMGRILVRGEPGNQILPQLTP 120 Query: 121 MAGEIVIDKPGKGAFYATDLHAQLQERGITHLLVAGVTTEVCVQTSMREANDRGYECLVV 180 MAGEIVIDKPGKGAFYATDLHAQLQERGITHLLVAGVTTEVCVQTSMREANDRGYECLVV Sbjct: 121 MAGEIVIDKPGKGAFYATDLHAQLQERGITHLLVAGVTTEVCVQTSMREANDRGYECLVV 180 Query: 181 EDACASYFPAFHRATLDMLTAQGGIVGWRAPLAQLQSAVTAYAGEHP 227 EDACASYFPAFHRATLDMLTAQGGIVGWRAPLAQLQSAVTAYAGEHP Sbjct: 181 EDACASYFPAFHRATLDMLTAQGGIVGWRAPLAQLQSAVTAYAGEHP 227 >gi|300310742|ref|YP_003774834.1| cysteine hydrolase falmily protein [Herbaspirillum seropedicae SmR1] Length = 231 Score = 213 bits (543), Expect = 8e-57, Method: Compositional matrix adjust. Identities = 110/227 (48%), Positives = 147/227 (64%), Gaps = 6/227 (2%) Query: 1 MIRIDAFPYPYQ--FHPRSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLA 58 I+ + +P+PY P +TAL+VIDMQ DF GG+ +G D+ A + + +LA Sbjct: 5 FIQAEPYPWPYDGALTPANTALIVIDMQTDFCGIGGYVDKMGYDLSLTRAPIEPIRKVLA 64 Query: 59 LARQAQMLVVHTRESHLPDLSDCPRAKRLR----GNPTLGIGDVGPMGRILVRGEPGNQI 114 R ++HTRE H PDLSD P KR R G +GIGD GP GRILVRGEPG +I Sbjct: 65 AMRAGGYTIIHTREGHRPDLSDLPANKRWRSRQIGANGVGIGDAGPCGRILVRGEPGWEI 124 Query: 115 LPQLAPMAGEIVIDKPGKGAFYATDLHAQLQERGITHLLVAGVTTEVCVQTSMREANDRG 174 +P+LAP+AGEI+IDKPGKG+F ATDL L RGI ++++ G+TT+VCV T+MREANDRG Sbjct: 125 IPELAPIAGEIIIDKPGKGSFCATDLEMILHTRGIRNIVLTGITTDVCVHTTMREANDRG 184 Query: 175 YECLVVEDACASYFPAFHRATLDMLTAQGGIVGWRAPLAQLQSAVTA 221 +EC+++ D C + H A L M+ QGG+ G A A L + + A Sbjct: 185 FECVMLSDCCGATDYNNHLAALSMIKMQGGVFGAVADSAALIAVIGA 231 >gi|300310658|ref|YP_003774750.1| 2,3-dihydro-2,3-dihydroxybenzoate synthetase [Herbaspirillum seropedicae SmR1] Length = 207 Score = 70.9 bits (172), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 57/177 (32%), Positives = 80/177 (45%), Gaps = 17/177 (9%) Query: 20 LVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLALARQAQMLVVHTRESHLPDLS 79 L+++D F + FG GN + + +LLA ARQ V H+R D + Sbjct: 25 LLIVDFVNSFADPQQFGG--GN----IPQAIERTRSLLAHARQQGWPVAHSRIVFADDDA 78 Query: 80 DCPRAKRLRGNPTLGIGDVGPMGRILVRGEPGNQILPQLAPMAGEIVIDKPGKGAFYATD 139 D + + G G+ L PG+ I+PQLAP GE+V+ K AF+ T Sbjct: 79 D----RNIFGMKVPGM-------VTLKEDLPGSAIVPQLAPANGELVVRKTVPSAFFGTS 127 Query: 140 LHAQLQERGITHLLVAGVTTEVCVQTSMREANDRGYECLVVEDACASYFPAFHRATL 196 L L + G+ LLVAG T CV+ S+ +A G+ LVV D H A L Sbjct: 128 LAPWLTQHGVQTLLVAGAVTSGCVRASVVDAMSWGFRPLVVSDCVGDRALGPHEANL 184 >gi|300313872|ref|YP_003777964.1| 2,3-dihydro-2,3-dihydroxybenzoate synthetase [Herbaspirillum seropedicae SmR1] Length = 176 Score = 56.2 bits (134), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 36/108 (33%), Positives = 56/108 (51%), Gaps = 9/108 (8%) Query: 105 LVRGEPGNQILPQLAPMAGEIVIDKPGKGAFYATDLHAQLQERGITHLLVAGVTTEVCVQ 164 L RG G Q++P L ++ + K G AF+ T+L A L+ R I+ L++ G+ TE CV+ Sbjct: 56 LQRGSEGWQLMPGLHQQPEDLHLPKRGSDAFHHTELQAMLKMRHISALVICGMQTEYCVE 115 Query: 165 TSMREANDRGYECLVVEDA---------CASYFPAFHRATLDMLTAQG 203 +++R A GY ++ DA + A H ATL L + G Sbjct: 116 STVRRALALGYPVMLAADAHTTAPNGVLTVAQVIAHHHATLAGLGSYG 163 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 760,075 Number of extensions: 31841 Number of successful extensions: 96 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 94 Number of HSP's successfully gapped: 5 Length of query: 227 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 145 Effective length of database: 1,231,600 Effective search space: 178582000 Effective search space used: 178582000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)