BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0050.p (964 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herb... 1999 0.0 gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspi... 184 2e-47 >gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 964 Score = 1999 bits (5179), Expect = 0.0, Method: Compositional matrix adjust. Identities = 962/964 (99%), Positives = 962/964 (99%) Query: 1 MSNNESSAKPGKPEKAGKAPSKYPVNMTETAFPMRGDLAKREPQWVKQWQEKKVYERIRK 60 MSNNESSAKPGKPEKAGKAPSKYPVNMTETAFPMRGDLAKREPQWVKQWQEKKVYERIRK Sbjct: 1 MSNNESSAKPGKPEKAGKAPSKYPVNMTETAFPMRGDLAKREPQWVKQWQEKKVYERIRK 60 Query: 61 ASAGRPKFILHDGPPYANGEIHLGHAVNKILKDMIVKARNMAGFDAQYVPGWDCHGMPIE 120 ASAGRPKFILHDGPPYANGEIHLGHAVNKILKDMIVKARNMAGFDAQYVPGWDCHGMPIE Sbjct: 61 ASAGRPKFILHDGPPYANGEIHLGHAVNKILKDMIVKARNMAGFDAQYVPGWDCHGMPIE 120 Query: 121 IQIEKKYGKNLPVAEVQAKGRAYANEQIELQKKDFIRLGVLGEWDNPYKTMNFSNEADEL 180 IQIEKKYGKNLPVAEVQAK RAYANEQI LQKKDFIRLGVLGEWDNPYKTMNFSNEADEL Sbjct: 121 IQIEKKYGKNLPVAEVQAKARAYANEQIALQKKDFIRLGVLGEWDNPYKTMNFSNEADEL 180 Query: 181 RALGKILEKGYVYRGLKPVNWCFDCGSALAEAEVEYQDKRDPAIDVGFPFAEHDKVATAF 240 RALGKILEKGYVYRGLKPVNWCFDCGSALAEAEVEYQDKRDPAIDVGFPFAEHDKVATAF Sbjct: 181 RALGKILEKGYVYRGLKPVNWCFDCGSALAEAEVEYQDKRDPAIDVGFPFAEHDKVATAF 240 Query: 241 GLTDLPTRHGYIVIWTTTPWTIPANQALNVHPEFEYALVETSRNGEATLLILAKDLVASC 300 GLTDLPTRHGYIVIWTTTPWTIPANQALNVHPEFEYALVETSRNGEATLLILAKDLVASC Sbjct: 241 GLTDLPTRHGYIVIWTTTPWTIPANQALNVHPEFEYALVETSRNGEATLLILAKDLVASC 300 Query: 301 LERFGLEGKIIATTTGEKLSLIRFHHPLAKADAGYDRLSPVFLGEYVTADSGTGIVHSSP 360 LERFGLEGKIIATTTGEKLSLIRFHHPLAKADAGYDRLSPVFLGEYVTADSGTGIVHSSP Sbjct: 301 LERFGLEGKIIATTTGEKLSLIRFHHPLAKADAGYDRLSPVFLGEYVTADSGTGIVHSSP 360 Query: 361 AYGIEDFVSCKAHGMKDDDILNPVMGDGRYASWLPLFAGLTIWEASKPICDKLEEIGSLF 420 AYGIEDFVSCKAHGMKDDDILNPVMGDGRYASWLPLFAGLTIWEASKPICDKLEEIGSLF Sbjct: 361 AYGIEDFVSCKAHGMKDDDILNPVMGDGRYASWLPLFAGLTIWEASKPICDKLEEIGSLF 420 Query: 421 KLIMFSHSYMHCWRHKSPIIYRATSQWFASMDNTPKDGGPSLRQTALQGIEDTAFFPGWG 480 KLIMFSHSYMHCWRHKSPIIYRATSQWFASMDNTPKDGGPSLRQTALQGIEDTAFFPGWG Sbjct: 421 KLIMFSHSYMHCWRHKSPIIYRATSQWFASMDNTPKDGGPSLRQTALQGIEDTAFFPGWG 480 Query: 481 KARLHGMIANRPDWTLSRQRQWGVPMAFFVHKETGELHPRTPELLEQIAQRIEQGGIEAW 540 KARLHGMIANRPDWTLSRQRQWGVPMAFFVHKETGELHPRTPELLEQIAQRIEQGGIEAW Sbjct: 481 KARLHGMIANRPDWTLSRQRQWGVPMAFFVHKETGELHPRTPELLEQIAQRIEQGGIEAW 540 Query: 541 QALDPKELLGDEADQYLKNKDTLDVWFDSGSTHQTVLRGSHRQELQFPADLYLEGSDQHR 600 QALDPKELLGDEADQYLKNKDTLDVWFDSGSTHQTVLRGSHRQELQFPADLYLEGSDQHR Sbjct: 541 QALDPKELLGDEADQYLKNKDTLDVWFDSGSTHQTVLRGSHRQELQFPADLYLEGSDQHR 600 Query: 601 GWFHSSLLTSSMLNGRAPYKALLTHGFVVDGEGRKMSKSLKNGVEPQKVADSLGAEILRL 660 GWFHSSLLTSSMLNGRAPYKALLTHGFVVDGEGRKMSKSLKNGVEPQKVADSLGAEILRL Sbjct: 601 GWFHSSLLTSSMLNGRAPYKALLTHGFVVDGEGRKMSKSLKNGVEPQKVADSLGAEILRL 660 Query: 661 WVASSDYSGEISISEEILKRVTEAYRRIRNTLRFLLANTSDFDPAQHAVPMAELLEIDRY 720 WVASSDYSGEISISEEILKRVTEAYRRIRNTLRFLLANTSDFDPAQHAVPMAELLEIDRY Sbjct: 661 WVASSDYSGEISISEEILKRVTEAYRRIRNTLRFLLANTSDFDPAQHAVPMAELLEIDRY 720 Query: 721 AIANMARLQGEVLAHFETYEFHPVVAKLQMYCSEDLGGFYLDILKDRLYTSGVDSAARRS 780 AIANMARLQGEVLAHFETYEFHPVVAKLQMYCSEDLGGFYLDILKDRLYTSGVDSAARRS Sbjct: 721 AIANMARLQGEVLAHFETYEFHPVVAKLQMYCSEDLGGFYLDILKDRLYTSGVDSAARRS 780 Query: 781 AQTAVWHITQSLLRLMAPILSFTAEEAWSFFAGKEAFEASGETIFSQVYYALPEVADAQG 840 AQTAVWHITQSLLRLMAPILSFTAEEAWSFFAGKEAFEASGETIFSQVYYALPEVADAQG Sbjct: 781 AQTAVWHITQSLLRLMAPILSFTAEEAWSFFAGKEAFEASGETIFSQVYYALPEVADAQG 840 Query: 841 LLAKFAVLHEVRAAVTKQLEEVRIAGSIGSSLQAEVELKVAGEKAALLESLGDDLKFVLI 900 LLAKFAVLHEVRAAVTKQLEEVRIAGSIGSSLQAEVELKVAGEKAALLESLGDDLKFVLI Sbjct: 841 LLAKFAVLHEVRAAVTKQLEEVRIAGSIGSSLQAEVELKVAGEKAALLESLGDDLKFVLI 900 Query: 901 TSAARVTRVASVEEEAVVVTPSAHQKCERCWHYRADVGSHAEHAGLCGRCVSNLFGQGEK 960 TSAARVTRVASVEEEAVVVTPSAHQKCERCWHYRADVGSHAEHAGLCGRCVSNLFGQGEK Sbjct: 901 TSAARVTRVASVEEEAVVVTPSAHQKCERCWHYRADVGSHAEHAGLCGRCVSNLFGQGEK 960 Query: 961 RQFA 964 RQFA Sbjct: 961 RQFA 964 >gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 971 Score = 184 bits (468), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 213/923 (23%), Positives = 372/923 (40%), Gaps = 172/923 (18%) Query: 39 AKREPQWVKQWQEKKVYERIRKASAGRPKFILHDGPPYANGEIHLGHAVNKILKDMIVKA 98 A+ E W ++W+++ + A +P F + PP G +H+GHA N+ + D + + Sbjct: 45 AEIEKFWREEWEKRGYFAAT--TDAEKPSFSIQLPPPNVTGTLHMGHAFNQTIMDGLTRY 102 Query: 99 RNMAGFDAQYVPGWDCHGMPIEIQIEKKYGKNLPVAEVQAKGRAYANEQIELQKKD---- 154 M G++ ++PG D G+ +I +E++ V+ + + E ++K Sbjct: 103 HRMRGYNTAWIPGTDHAGIATQIVVERQLDAQ-KVSRHDLGREKFVEKVWEWKEKSGSTI 161 Query: 155 ---FIRLGVLGEWDNPYKTMNFSNEADELRALGKILEKGYVYRGLKPVNWCFDCGSALAE 211 R+G +W Y TM+ ++ E+G +YRG + VNW G+A+++ Sbjct: 162 TGQMRRMGASTDWAREYFTMDPKMSKVVTEVFVRLYEQGLIYRGKRLVNWDPVLGTAVSD 221 Query: 212 AEVEYQDKRDPAIDVGFPFAEHDKVATAFGLTDLPTRHGYIVIWTTTPWTIPANQALNVH 271 EV +++ + +P A+ G+IV+ TT P T+ + A+ V Sbjct: 222 LEVVSEEEDGSMWHIKYPLADGS---------------GHIVVATTRPETMLGDVAVAVD 266 Query: 272 PEFE-YALVETSRNGEATLLILAKDLVASCLERFGLEGKIIATTTGEKLSLIRFHHPLAK 330 P E YA L GK+I KL L+ Sbjct: 267 PTDERYA---------------------------ALVGKMI------KLPLV-------- 285 Query: 331 ADAGYDRLSPVFLGEYVTADSGTGIVHSSPAYGIEDFVSCKAHGMKDDDILNPVMGDGRY 390 R P+ EYV + GTG V +PA+ D+ H ++ + Sbjct: 286 -----GREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEMISVFTLKATINEN 340 Query: 391 ASWLPLFAGLTIWEASKPICDKLEEIGSLFKLIMFSHSYMHCWRHKSPIIYRA--TSQWF 448 A + GL +EA K I L+ +G L ++ H M ++ ++ T QWF Sbjct: 341 AP--EKYQGLDRFEARKQIVADLDALGLLEQVK--PHKLMVPRGDRTGVVIEPMLTDQWF 396 Query: 449 ASMDNTPKDG----GPSLRQTALQGIE--DTAFFPGWGKARLHGMIANRPDWTLSRQRQW 502 +M +G G S+ +TAL+ + + F P + + N DW +SRQ W Sbjct: 397 VAMSKPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNNIQDWCISRQLWW 456 Query: 503 GVPMAFFVHKETGELHPRTPELLEQIAQRIEQGGIEAWQALDPKELLGDEADQYLKNKDT 562 G + + ++ G RT E R + GG +A+ +++D Sbjct: 457 GHQIPAWYDEDGGIYVARTEE-----EARAQAGG----RAVK-------------RDEDV 494 Query: 563 LDVWFDSGSTHQTVL---RGSHRQELQFPADLYLEGSDQHRGWFHSSLLTSSMLNGRAPY 619 LD WF S + L + +L P+ + + G D W ++ ++ G+ P+ Sbjct: 495 LDTWFSSALVPFSTLGWPEETPDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKVPF 554 Query: 620 KALLTHGFVVDGEGRKMSKSLKNGVEPQKVADSLGAE--ILRLWVASSDYSGEISISEEI 677 + HG V D G+KMSKS N ++P + D +G + + + V + SI + Sbjct: 555 DTVYVHGLVRDASGQKMSKSKGNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKAT 614 Query: 678 LK--------------RVT------------------EAYRRIRNTL----RFLLANT-- 699 K R T E YR N L RF+L NT Sbjct: 615 RKEFADGIPAFGTDALRFTFASLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMNTEG 674 Query: 700 --SDFDPAQHAVPMAELLEI---DRYAIANMARLQGEVLAHFETYEFHPVVAKLQMYCSE 754 FD + V E L+ DR+ ++ + R + EV F Y F + + + + Sbjct: 675 QDCGFDGHEKGVCDKEKLQFSKADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKFVWD 734 Query: 755 DLGGFYLDILKDRLYTSGVDSAARRSAQTAVWHITQSLLRLMAPILSFTAEEAWSFFA-- 812 + +YL++ K ++ + + A +R+ + + + +S+LRL P+L F E+ W A Sbjct: 735 EYCDWYLEMAKAQIQSG--NEAQQRATRRTLLRVLESVLRLAHPVLPFITEQLWQSVAPL 792 Query: 813 GKEAFEASGETIFSQVYYALPEV----ADAQGLLAKFAVLHEVRAAVTKQLEEVRIAGSI 868 + +G++I Q Y P++ A+A+ +A+F A T +R + Sbjct: 793 TDRKPDPAGDSIMRQA-YPRPDLEKIDAEAEAWVAQF-------KAYTDACRNLRGEMQL 844 Query: 869 GSSLQAEVELKVAGEKAALLESL 891 SL+ V L V A+ +ES Sbjct: 845 APSLR--VPLIVEAADASAVESF 865 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,947,684 Number of extensions: 126143 Number of successful extensions: 357 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 350 Number of HSP's successfully gapped: 5 Length of query: 964 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 870 Effective length of database: 1,174,780 Effective search space: 1022058600 Effective search space used: 1022058600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)